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| 1 | 禾谷镰孢菌高产毒菌株的构建显示文摘为研究禾谷镰孢菌Fusarium graminearum Schw.单端孢霉烯族毒素生物合成基因(产毒基因)在寄主体内的表达,作者构建了带报告基因GUS((-葡糖苷酸酶基因)的质粒pGUSTRI6P5,并通过对野生型菌株的转化获得禾谷镰孢高产毒菌株。该质粒含有由TRI5(禾谷镰孢单端孢霉二烯合酶基因)启动子(TRI5 Prom)驱动的GUS基因编码区、潮霉素B抗性基因和拟枝孢镰孢F. sporotrichioides的产毒调控基因TRI6(FSTRI6)。用pGUSTRI6P5转化野生型菌株GZ3639后,在含潮霉素 B的培养基上选取抗性菌落,单孢分离获单孢菌株(转化子)。在GYEP(葡萄糖-酵母粉-蛋白胨)液体培养基上,转化子B4-1和B16-1的GUS比活力强,15-AcDON(15-乙酰脱氧雪腐镰刀菌烯醇)产量高,且两者呈正相关(相关系数(r)分别为0.9839和0.9523)。B4-1和B16-1两个转化子可作为研究禾谷镰孢与其寄主相互作用的工具菌株。 | 陈利锋 Thomas M HOHN | 2001 | 菌物系统2001，20，3： | 8 |
| 2 | Antifibrogenic effects of vitamin D derivatives on mouse pancreatic stellate cells显示文摘AIM To study the molecular effects of three different D-vitamins, vitamin D2, vitamin D3 and calcipotriol, in pancreatic stellate cells(PSCs).METHODS Quiescent PSCs were isolated from mouse pancreas and activated in vitro by seeding on plastic surfaces. The cells were exposed to D-vitamins as primary cultures(early-activated PSCs) and upon re-culturing(fullyactivated cells). Exhibition of vitamin A-containing lipid droplets was visualized by oil-red staining. Expression of α-smooth muscle actin(α-SMA), a marker of PSC activation, was monitored by immunofluorescence and immunoblot analysis. The rate of DNA synthesis was quantified by 5-bromo-2'-deoxyuridine(Brd U) incorporation assays. Real-time PCR was employed to monitor gene expression, and protein levels of interleukin-6(IL-6) were measured by ELISA. Uptake of proline was determined using 18 F-proline.RESULTS Sustained culture of originally quiescent PSCs inducedcell proliferation, loss of lipid droplets and exhibition of stress fibers, indicating cell activation. When added to PSCs in primary culture, all three D-vitamins diminished expression of α-SMA(to 32%-39% of the level of control cells; P < 0.05) and increased the storage of lipids(scores from 1.97-2.15 on a scale from 0-3; controls: 1.49; P < 0.05). No such effects were observed when D-vitamins were added to fully-activated cells, while incorporation of Brd U remained unaffected under both experimental conditions. Treatment of re-cultured PSCs with D-vitamins was associated with lower expression of IL-6(-42% to-49%; P < 0.05; also confirmed at the protein level) and increased expression of the vitamin D receptor gene(209%-321% vs controls; P < 0.05). There was no effect of D-vitamins on the expression of transforming growth factor-β1 and collagen type 1(chain α1). The lowest uptake of proline, a main component of collagen, was observed in calcipotriol-treated PSCs.CONCLUSION The three D-vitamins inhibit, with similar efficiencies, activation of PSCs in vitro, but cannot reverse the phenotype once the cells are fully activated. | Peter Wallbaum Sarah Rohde Luise Ehlers Falko Lange Alexander Hohn Carina Bergner Sarah Marie Schwarzenbock Bernd Joachim Krause Robert Jaster | 2018 | World Journal of Gastroenterology2018，24，2： | 3 |
| 3 | Evolutionary history and phylogeography of Scots pine (Pinus sylvestris L.) in Europe based on molecular markers显示文摘In this review we summarized recent historical records and molecular studies on evolutionary history and phylogeography of Scots pine with focus on the European highly fragmented distribution area of the species. Fossilized pollen, plant micro-and macrofossil records provided evidences on the large-scale species' range shifts and demographic changes during the Quaternary. Populations of Scots pine were documented both in the glacial(incl.full glaciation) and interglacial periods. Recolonization of Europe after the glaciation originated from the(Sub)Mediterranean areas like the Balkan Peninsula but also from around the Eastern Alps and the surroundings of the Danube plain. Fennoscandia and northern European Baltic regions were most probably colonized from two main directions, from Western Europe and from the Russian Plain. Modern history of Scots pine was hardly affected by anthropogenic activities that started to strengthen in the Bronze and Iron Age. Along with the fossil records,molecular genetic tools were used to infer the origin and putative history including migration, differentiation and demography of the species. In this paper we compiled the major publications(30) of molecular genetic studies of the past 20 years derived from distinctly inherited organelle genomes(mitochondrial, chloroplast, nuclear) revealed by different marker systems(mt DNA-cox1,-nad1,-nad3,-nad7, ISSR, cp SSR, n SSR, B-SAP, SNP). It is important to consider that different phylogeographic patterns can be drawn by the analysis of different DNA marker types.Accordingly the use of more than one marker simultaneously outlines the most sophisticated phylogeographical pattern on the genetic lineages and can reveal high differentiation of the European distribution. Combined marker systems and markers derived from coding sequences have also been used to detect species' phylogeographic patterns,but these were rarely applied to Scots pine. Although new molecular techniques can provide higher resolution data for populations, the reviewed results can shape the direction of further studies. | Endre Gy. To′th Zolta′n A. Kobolkuti Andrzej Pedryc Ma′ria Hohn | 2017 | Journal of Forestry Research2017，28，4： | 3 |
| 4 | Regional distribution of brain-derived neurotrophic factor mRNA in the adult mouse brain显示文摘 | Hofer M Pagliusi SR Hohn A | 1990 | EM BO1990，9，： | 2 |
| 5 | Size and shape dependence of the exchange-bias field in exchange-coupled ferrimgnetic bilayers显示文摘 | ZIESE M HoHNE R BOLLERO A | 2005 | The European Physical Journal B2005，45，2： | 1 |
| 6 | A randomized,trial of continuous intravenous versus hepatic intraarterial floxaridine in patients with colorectal cancer metastatic to the liver:Northern California Oncology Group trial显示文摘 | Hohn DC Stagg RJ Friedman MA | 1989 | J Clin Oncol1989，7，11： | 1 |
| 7 | An all chemical solution deposition approach for the growth of highly textured CeO2 cap layers on La2Zr2OT-buffer long lengths of biaxially textured Ni-W substrates for YBCO coated conductors 显示文摘 | ENGEL S KNOTH K HOHNE R | 2005 | Super Sci Tech2005，18，10： | 1 |
| 8 | Blood flow and leukocyte mobilization in infection:A comparison between ischemic and wellperfused flaps显示文摘 | Feng L J Price DC Hohn D | 1983 | Plast Surg Forum1983，86，： | 1 |
| 9 | Identification of differentially expressed genes in human trophoblast cells by differential display RT-PCR 显示文摘 | Huch G Hohn HP Denker HW | 1998 | Placenta1998，19，8： | 1 |
| 10 | Interactive 3D segmentation of MRI and CT volumes using morphological operations 显示文摘 | HOHNE K HANSON W | 1992 | Journal of Computer Assisted Tomography1992，16，2： | 1 |
| 11 | Human pharmcokinetics and safety evaluation of sonovue, a newcontrast agent for ultrasound imaging显示文摘 | Morel DR Sehwieger I Hohn L | 2000 | Invest Radiol2000，35，： | 1 |
| 12 | Ontogeny of fear-, anxiety- and depressionrelated behavior across adolescence in C57BL/6J mice显示文摘 | Hefner K Hohnes A | 2007 | Behav Brain Res2007，17，6： | 1 |
| 13 | The haemody-namic and catecholamine response to xenon/remifentanil an-aesthesia in Beagle dogs显示文摘 | Francis R C Reyle-Hahn M S Hohne C | 2008 | Lab Anim2008，42，3： | 1 |
| 14 | single lung transplantation for adult respiratory distress syndrome after paraquat poisoning显示文摘 | Licker M Schweizer A Hohn L | 1998 | Thorax1998，53，7： | 1 |
| 15 | Catalytic oxidation of methanol on nanoscale copper oxide and nickel oxide显示文摘 | Gu Zheng Hohn Keith L | 2004 | Ind Eng Chem Res2004，43，： | 1 |
| 16 | Procaleitonin- guided algorithm to reduce length of antibiotic therapy in patients with severe sepsis and septic shock 显示文摘 | Hohn A Schroeder S Gehrt A | 2013 | BMC Infect Dis2013，，13： | 1 |
| 17 | A novel 9-class auditory ERP paradigm driving a predictive text entry system 显示文摘 | HOHNE J SCHREUDER M BLANKERTZ B | 2011 | Frontiers in Neuroscience2011，99，5： | 1 |
| 18 | Use of Fusarium graminearum transformed with gfp to follow infection patterns in barley and Arabidopsis 显示文摘 | Skadsen RW Hohn TM | 2004 | Physiological and Molecular Plant Pathology2004，64，1： | 1 |
| 19 | Mucins expression by transitional cell carcinomas of the bladder显示文摘 | Walsh MD Hohn BG Thong W | 1994 | Br J Urol1994，73，2： | 1 |
| 20 | Prevention of central venous catheterrelated infections by using maximal sterile barrier precautions during insertion显示文摘 | Raad H Hohn DC Gilbreath BJ | | 0，，04： | 1 |
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