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| 1 | Isolation and characterisation of human gingival margin-derived STRO-1/MACS^+ and MACS^-cell populations显示文摘Recently,gingival margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting(MACS) showed remarkable periodontal regenerative potential in vivo.As a second-stage investigation,the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive(MACS^+) and STRO-1-negative(MACS^-) cell populations from the human free gingival margin.Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies.Subsequently,the MACS^+ and MACS^- cell fractions were characterized by flow cytometry for expression of CD14,CD34,CD45,CD73,CD90,CD105,CD146/MUC18 and STRO-1.Colony-forming unit(CFU) and multilineage differentiation potential were assayed for both cell fractions.Mineralisation marker expression was examined using real-time polymerase chain reaction(PCR).MACS^+ and MACS- cell fractions showed plastic adherence.MACS^+ cells,in contrast to MACS- cells,showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs(P<0.01).More than 95%of MACS^+ cells expressed CD105,CD90 and CD73;lacked the haematopoietic markers CD45,CD34 and CD14,and expressed STRO-1 and CD146/MUC18.MACS- cells showed a different surface marker expression profile,with almost no expression of CD14 or STRO-1,and more than 95%of these cells expressed CD73,CD90 and CD146/MUC18,as well as the haematopoietic markers CD34 and CD45 and CD105.MACS^+ cells could be differentiated along osteoblastic,adipocytic and chondroblastic lineages.In contrast,MACS- cells demonstrated slight osteogenic potential.Unstimulated MACS^+ cells showed significantly higher expression of collagen I(P<0.05) and collagen III(P<0.01),whereas MACS^- cells demonstrated higher expression of osteonectin(P<0.05;MannWhitney).The present study is the first to compare gingival MACS^+ and MACS- cell populations demonstrating that MACS^+ cells,in contrast to MACS- cells,harbour stem/progenitor cell characteristics.This study also validates the effectiveness of the STRO-l/MACS^+technique for the isolation of gingival stem/progenitor cells.Human free gingival margin-derived STRO-1/MACS^+ cells are a unique renewable source of multipotent stem/progenitor cells. | Karim M Fawzy El-Sayed Sebastian Paris Christian Graetz Neemat Kassem Mohamed Mekhemar Hendrick Ungefroren Fred Fndrich Christof Drfer | 2015 | International Journal of Oral Science2015，7，2： | 6 |
| 2 | X-ray measurement of laser fusion targets using least squares fitting显示文摘 | Singleton R M Weinstein B W Hendricks C D | 1979 | Appl Opt1979，18，24： | 2 |
| 3 | Amercian college of radiology guideline for breast cancer screening 显示文摘 | Feig SA D'Orsi CJ Hendrick KE | 1998 | AJR1998，171，1： | 1 |
| 4 | Feedback Control of Von Karman Vortex Shedding Behind a Circular Cylinder at Low Reynolds Numbers显示文摘 | Park D S Ladd D M Hendricks E W | 1994 | Physics of Fluids1994，6，7： | 1 |
| 5 | Homing of hatchery-reared American shad to the Lehigh River, a tributary to the Delaware River) 显示文摘 | Hendricks M L Hoopes R 1 Arnold D A | 2002 | North American Journal of Fisheries Management2002，22，： | 1 |
| 6 | Wound healing after laser surgery显示文摘 | HENDRICK D A MEYERS A | 1995 | Otolaryngol Clin North Am1995，5，： | 1 |
| 7 | Fine root branch orders respond differentially to carbon source-sink manipulations in a longleafpine forest 显示文摘 | GUO D L MITCHELL R J HENDRICKS J J | 2004 | Oecologia2004，140，： | 1 |
| 8 | Protective effect of dietary tomatine against dibenzopyrene (OBP)-induced liver and stomach tumors in rainbow trout 显示文摘 | Friedman M McQuistan T Hendricks J D | 2007 | Molecular Nutrition and Food Research2007，51，12： | 1 |
| 9 | American College of Radiology guidelines for breast caneer screening 显示文摘 | Feig SA D'Orsi CJ Hendrick RE | 1998 | AJR Am J Roentgenol1998，171，1： | 1 |
| 10 | Assessing the role of fine roots in carbon and nutrient cycling显示文摘 | Hendricks J J Nadelhoffer K J Aber J D | 1993 | Trends in Ecology & Evolution1993，8，5： | 1 |
| 11 | Dynamic inversion: an evolving methodology for flight control design显示文摘 | Enns D Bugajski D Hendrick R | 1994 | International Journal of Control1994，59，1： | 1 |
| 12 | Mesenchymal stem cells as vehicles for gene delivery显示文摘 | Mosca JD Hendricks JK Buyaner D | 2000 | Clin Orthop Relat Res2000，，： | 1 |
| 13 | Clinical comparison of full-field digital mammography and screen-film mammography for detection of breast cancer显示文摘 | Lewin JM D'Orsi CJ Hendrick RE | | 0，，03： | 1 |
| 14 | Mathematical programming and the location of fire companies for the Denver fire department显示文摘 | Plane D Hendrick T | 1977 | Operations Research1977，25，4： | 1 |
| 15 | Abnormal Fhit expression in malignant and premalignant lesions of the cervix 显示文摘 | Birrer M J Hendricks D Farley J | 1999 | Cancer Res1999，59，20： | 1 |
| 16 | Biogeochemical response of forest ecosystems to simulated chronic nitrogen deposition显示文摘 | MAGILL A H ABER J D HENDRICKS J J | 1997 | Ecological Applications1997，7，2： | 1 |
| 17 | The rationality struggle: Illustration,s from financial markets 显示文摘 | Patel J Richard Z Hendricks D | 1991 | American Economic Review1991，81，2： | 1 |
| 18 | Fine root branch orders respond differentially to carbon source-sink manipulations in a longleafpine forest显示文摘 | Guo D L Mitchell R J Hendricks J J | 2004 | Oecologia2004，140，3： | 1 |
| 19 | Digital mammography显示文摘 | Lewin JM D'Orsi CJ Hendrick RE | 2004 | Radiol Clin North Am2004，42，5： | 1 |
| 20 | Prescribing practices of primarcare veterinary practitioners in dogs diagnosed with bacterial pyoderma显示文摘 | Summers J F Hendricks A Brodbelt D C | 2014 | BMC Vet Res2014，10，： | 1 |
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