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| 1 | Enhanced expression of interleukin-18 in serum and pancreas of patients with chronic pancreatitis显示文摘AIM: To investigate interleukin-18 (IL-18) in patients with chronic panreatitis (CP). METHODS: We studied 29 patients with CP and 30 healthy controls. Peripheral blood mononuclear cells (PBMC) were isolated and incubated with 50 mmol/L ethanol, lipopolysaccharide (LPS) (doses 25 g/L, 250 g/L, 2500 g/L) and both agents for 24 h. Levels of IL-18 in the supernatants, and levels of IL-18, IL-12, interferon (IFN)-γ and soluble CD14 in the serum were analysed by ELISA technique. Expression of IL-18 in PBMC was investigated by reverse-transcription (RT)-PCR. IL-18 protein levels in CP tissue and in normal pancreas were studied by ELISA technique. IL-18 levels in PBMC and pancreatic tissue were determined by Westernblot. Im-munohistochemistry for pancreatic IL-18 expression was performed. RESULTS: In patients, IL-18 serum levels were signifi-cantly enhanced by 76% (mean: 289.9 ± 167.7 ng/L) compared with controls (mean: 165.2 ± 43.6 ng/L; P < 0.0005). IL-12 levels were enhanced by 25% in patients (18.3 ± 7.3 ng/L) compared with controls (14.7 ± 6.8ng/L, P = 0.0576) although not reaching the statistical significance. IFN-γ and soluble CD14 levels were not in-creased. In vitro, LPS stimulated significantly and dose-dependently IL-18 secretion from PBMC. Incubation with ethanol reduced LPS-stimulated IL-18 secretion by about 50%. The mRNA expression of IL-18 in PBMC and the response of PBMC to ethanol and LPS was similar in CP patients and controls. In PBMC, no significant differences in IL-18 protein levels were detected between patients and controls. IL-18 protein levels were increased in CP tissues compared to normal pancreatic tissues. IL-18 was expressed by pancreatic acinar cells and by infiltrating inflammatory cells within the pancreas. CONCLUSION: IL-18 originates from the chronically inflammed pancreas and appears to be involved in the fibrotic destruction of the organ. | Alexander Schneider Stephan L Haas Ralf Hildenbrand Sren Siegmund Iris Reinhard Helmut Nakovics Manfred V Singer Peter Feick | 2006 | World Journal of Gastroenterology2006，12，40： | 4 |
| 2 | Challenges for land system science显示文摘 | Mark D.A. Rounsevell Bas Pedroli Karl-Heinz Erb Marc Gramberger Anne Gravsholt Busck Helmut Haberl S?ren Kristensen Tobias Kuemmerle Sandra Lavorel Marcus Lindner Hermann Lotze-Campen Marc J. Metzger David Murray-Rust Alexander Popp Marta Pérez-Soba Anett | 2012 | Land Use Policy2012，，4： | 2 |
| 3 | Glutathione and its role in cellular functions显示文摘 | Helmut S | 1999 | Free Radical Biology & Medicine1999，27，： | 1 |
| 4 | Unstiffened steel plate shear wall performance under cyclic loading显示文摘 | ADAM S L HELMUT G L P | 2000 | Journal of Structural Engineering2000，126，4： | 1 |
| 5 | MDCT angiography of the pulmonary arteries: influence of iodine flow concentrationon vesell attenuation and visualization显示文摘 | Helmut S Hannes AD Gerald AF | 2005 | AJR2005，184，6： | 1 |
| 6 | MDCT angiography of the pulmonary arteries:influence of iodine flow concentration on vesell attenuation and visualization 显示文摘 | Helmut S Hannes AD Gerald AF | 2005 | A JR2005，184，6： | 1 |
| 7 | Infraredspectroscopic investigations of selective catalytic reduction catalysts poisoned with arsenic oxide显示文摘 | FRIEDERIKE C L HELMUT S HELMUT K | 1996 | Appl Catal B1996，8，24： | 1 |
| 8 | Development and evaluation of an online CO2 evolution test and a multicomponent biodegradation test system显示文摘 | Uwe S Peter R Helmut S | 2004 | Applied and environmental microbiology2004，70，8： | 1 |
| 9 | Human CD4^+ CD25^+ regulatory T cells and infectious tolerance显示文摘 | Michael S Edga S Helmut J | 2004 | Transplantation2004，77，1： | 1 |
| 10 | An X-ray photo electron spectroscopy study of oxides of arsenic supported on Ti O2显示文摘 | FRIEDERIKE C L HELMUT S HELMUT K | 1991 | J Electron Spectrosc1991，57，30： | 1 |
| 11 | Renin-angiotensin system and atherosclerosis显示文摘 | Karsten G Helmut D Bernhard S | 2004 | Nephrol Dial Transplant2004，19，： | 1 |
| 12 | Effect of diabetes on low-erurinary tract symptoms in patients with benign prostatic hyperplasia 显示文摘 | Martin CM Ludwig M Helmut S | 2000 | J Urol2000，163，： | 1 |
| 13 | Response of endophytic bacterial communities in potato plant to infection with Erwinia carotovora subsp, atroseptica显示文摘 | BIRGIT R ULRIKE P HELMUT S | 2002 | Applied and Environment Microbiology2002，，： | 1 |
| 14 | Enzymology of the degradation of (di)chlorobenzenes by Xanthobacter flavus 14pl显示文摘 | CLAUDIA S HELMUT G | 1997 | Archives of Microbiology1997，167，6： | 1 |
| 15 | Life-stage-dependent sensitivity of zebrafish (Danio rerio) to estrogen exposure显示文摘 | Gerd M Helmut S | 2004 | Comparative Biochemistry and Physiology(Part C)2004，139，13： | 1 |
| 16 | Chemistry of aiologically important synthetic organoselenium compounds 显示文摘 | Govindasamy M Wolf Walther D M Helmut S | 2001 | Chem Rev2001，101，7： | 1 |
| 17 | Parametric airfoils and wings显示文摘 | HELMUT S | 1998 | Notes on Nu- merical Fluid Mechanics1998，68，： | 1 |
| 18 | Supercritical water oxidation: state of the art显示文摘 | Helmut S Johannes A | 1999 | Chem Eng Technol1999，22，11： | 1 |
| 19 | Up-converting nanoparticles显示文摘 | Markus H Helmut S | | 0，，50： | 1 |
| 20 | Prognostic value of E-cadherin expression in 413 gastric carcinomas显示文摘 | Helmut E G Wolfram M Ank S | 1996 | Int J Cancer1996，69，： | 1 |
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