|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Rice RING protein OsBBI1 with E3 ligase activity confers broad-spectrum resistance against Magnaporthe oryzae by modifying the cell wall defence显示文摘新兴的证据建议 E3 ligases 在多样的生物过程起关键作用,包括在植物的天生的有免疫力的回答。然而,在植物的 E3 ligase 参与的机制天生的免疫是不清楚的。我们报导那米饭基因, OsBBI1,与 E3 ligase 活动编码戒指手指蛋白质,调停用途广泛的疾病抵抗。OsBBI1 的表示被米饭强风真菌 Magnaporthe oryzae,以及化学 inducers 导致, benzothiadiazole 和水杨酸酸。生物化学的分析表明那 OsBBI1 蛋白质在 vitro 拥有 E3 ubiquitin ligase 活动。基因分析表明在一根 Tos17 插入线的 OsBBI1 功能的损失增加了危险性,当在转基因的植物的 OsBBI1 的 overexpression 授与提高的抵抗到 M 的多重赛跑时。oryzae。这显示 OsBBI1 对强风真菌调制用途广泛的抵抗。OsBBI1-overexpressing 植物由 M 在感染地点在房间墙中在酉分的混合物的房间和蛋白质的 cross-linking 显示出 H2O2 累积的高水平。oryzae 与相比野类型(WT ) 植物。房间墙在 OsBBI1-overexpressing 植物是更厚的并且在变异的植物更薄比在 WT 植物。我们的结果建议 OsBBI1 由修改房间墙防卫回答调制用途广泛的抵抗到强风真菌。OsBBI1 的功能的描述为在米饭对最破坏的疾病构造用途广泛的抵抗提供卓见进 E3 调停 ligase 的天生的免疫,和一个实际工具。 | Wei Li Sihui Zhong Guojun Li Qun Li Bizeng Mao Yiwen Deng Huijuan Zhang Longjun Zeng Fengming Song Zuhua He | 2011 | Cell Research2011，21，5： | 30 |
| 2 | BEAK-SHAPED GRAIN 1/TRIANGULAR HULL 1,a DUF640 gene,is associated with grain shape,size and weight in rice显示文摘Grain shape and size both determine grain weight and therefore crop yield.However,the molecular mechanisms controlling grain shape and size are still largely unknown.Here,we isolated a rice mutant,beak-shaped grain1(bsg1),which produced beak-shaped grains of decreased width,thickness and weight with a loosely interlocked lemma and palea that were unable to close tightly.Starch granules were also irregularly packaged in the bsg1 grains.Consistent with the lemma and palea shapes,the outer parenchyma cell layers of these bsg1 tissues developed fewer cells with decreased size.Map-based cloning revealed that BSG1 encoded a DUF640 domain protein,TRIANGULAR HULL 1,of unknown function.Quantitative PCR and GUS fusion reporter assays showed that BSG1 was expressed mainly in the young panicle and elongating stem.The BSG1 mutation affected the expression of genes potentially involved in the cell cycle and GW2,an important regulator of grain size in rice.Our results suggest that BSG1 determines grain shape and size probably by modifying cell division and expansion in the grain hull. | YAN DaWei ZHOU Ya YE ShengHai ZENG LongJun ZHANG XiaoMing HE ZuHua | 2013 | Science China(Life Sciences)2013，56，3： | 20 |
| 3 | Control of Rice Embryo Development, Shoot Apical Meristem Maintenance, and Grain Yield by a Novel Cytochrome P450显示文摘被子植物种子通常由二主要部分组成:胚胎和内乳。然而,分子的机制 underlyingembryo 和内乳发展仍然保持大部分未知,特别地在米饭,模型谷物。这里,我们报导 identificationand 米饭巨人胚胎(GE ) 基因的功能的描述。GE 的变化在种子导致了一个大胚胎,它被胚鳞房间的过多的扩大引起。embryonic 以后 growthof ge 幼苗严重地由于有缺点的射击被禁止顶端的分裂组织(SAM ) 维护。基于地图的克隆表明 thatGE 编码对 endoplasmic 蜂窝胃局部性的 CYP78A 亚科 P450 monooxygenase。GE 在 scutellar 上皮主要被表示,在胚胎和内乳之间的接口区域。Overexpressionof GE 支持了细胞增长并且提高了米饭植物生长和谷物产量,但是减少了胚胎尺寸,建议 GE 为协调米饭胚胎和内乳开发是批评的。而且,转基因的 Arabidopsis 植物 overexpressing AtCYP78A10,一个 GE 相当或相同的事物,也生产的更大的种子,在一起调整种子 development.Taken 为 P450s 的 CYP78A 亚科暗示一个保存角色,我们的结果显示 GE 在调整胚胎发展和 SAM maintenance.Key 词起关键作用: | Weibing Yang Mingjun Gao Xin Yin Jiyun Liu Yonghan Xu Longjun Zeng Qun Li Shubiao Zhang Junmin Wang Xiaoming Zhang Zuhua He | 2013 | Molecular Plant2013，6，6： | 16 |
| 4 | Isoliquiritigenin inhibits TGF-β1-induced fibrogenesis through activating autophagy via PI3K/AKT/mTOR pathway in MRC-5 cells显示文摘Isoliquiritigenin(ISL),a natural flavonoid derived from the root of liquorice,has been reported to possess anti-inflammatory and antioxidant activities.Previous studies have found that ISL plays a crucial role in anti-fibrosis of adipose tissue and renal tissue;however,its effect on pulmonary fibrogenesis has not been demonstrated.In this study,we aimed to explore the roles and the underlying mechanisms of ISL in TGF-β1-induced fibrogenesis using human lung fibroblast-derived MRC-5 cells.Cell proliferation and migration were determined by MTT and wound healing assay,respectively.The expression levels of alpha-smooth muscle actin(α-SMA),collagen type I alpha 1(COLIA1)and fibronectin(FN),microtubule-associated protein light chain 3(LC3)and related signaling molecules were detected by quantitative real-time PCR,western blot and immunofluorescence assay,correspondingly.EGFP-LC3 transfection was used for autophagy analysis.The results showed that ISL inhibited the TGF-β1-induced proliferation and migration,and down-regulated the expressions ofα-SMA,COLIA1 and FN.ISL treatment led to up-regulation of LC3 in TGF-β1-treated MRC-5 cells,accompanied by significant decrease in the phosphorylation levels of phosphatidylinositol 3-kinase(PI3K),protein kinase B(AKT),and mammalian target of rapamycin(mTOR).In addition,the inhibitory effects of ISL on TGF-β1-induced fibrogenic features in MRC-5 cells were enhanced by pretreatment with autophagy activator Rapmycin and PI3K/AKT inhibitor LY294002 and reversed by autophagy inhibitor 3-methyladenine and PI3K/AKT activator IGF-1.Taken together,our results demonstrated that ISL could attenuate the fibrogenesis of TGF-β1-treated MRC-5 cells by activating autophagy via suppressing the PI3K/AKT/mTOR pathway.Therefore,ISL holds a great potential to be developed as a novel therapeutic agent for the treatment of pulmonary fibrosis. | Jinjuan He Hao Peng Meifang Wang Ying Liu Xingrong Guo Bin Wang Longjun Dai Xueqin Cheng Zhongji Meng Leyong Yuan Fenglin Cai Yijun Tang | 2020 | Acta Biochimica et Biophysica Sinica2020，52，8： | 16 |
| 5 | A DELLA protein complex controls the arbuscular mycorrhizal symbiosis in plants显示文摘 | Nan Yu Dexian Luo Xiaowei Zhang Junzhong Liu Wanxiao Wang Yue Jin Wentao Dong Jiyun Liu Huan Liu Weibing Yang Longjun Zeng Qun Li Zuhua He Giles E D Oldroyd Ertao Wang | 2014 | Cell Research2014，24，1： | 9 |
| 6 | Characterization and mapping of a novel mutant sms1 (senescence and male sterility 1) in rice显示文摘Plant senescence plays diverse important roles in development and environmental responses.However,the molecular basis of plant senescence is remained largely unknown.A rice spontaneous mutant with the character of early senescence and male sterility (sms) was found in the breeding line NT10-748.In order to identify the gene SMS1 and the underlying mechanism,we preliminarily analyzed physiological and biochemical phenotypes of the mutant.The mutant contained lower chlorophyll content compared with the wild type control and was severe male sterile with lower pollen viability.Genetic analysis showed that the mutant was controlled by a single recessive gene.By the map-based cloning approach,we fine-mapped SMS1 to a 67 kb region between the markers Z3-4 and Z1-1 on chromosome 8 using 1,074 F2 recessive plants derived from the cross between the mutant sms1 (japonica) × Zhenshan 97 (indica),where no known gene involved in senescence or male sterility has been identified.Therefore the SMS1 gene will be a novel gene that regulates the two developmental processes.The further cloning and functional analysis of the SMS1 gene is under way. | Wenyi Yan Shenghai Ye Qingsheng Jin Longjun Zeng Yu Peng Dawei Yan Weibing Yang Donglei Yang Zuhua He Yanjun Dong Xiaoming Zhang | 2010 | Journal of Genetics and Genomics2010，37，1： | 6 |
| 7 | Prevalence, risk factors and impact of gastroesophageal reflux disease symptoms: A population-based study in South China显示文摘 | Minhu Chen Lishou Xiong Huixin Chen Angao Xu Longjun He Pinjin Hu | 2005 | Scandinavian Journal of Gastroenterology2005，，7： | 2 |
| 8 | Enumeration and molecular characterization of circulating tumor cell using an in vivo capture system in squamous cell carcinoma of head and neck显示文摘Objective: Detection rate and isolation yield of circulating tumor cell(CTC) are low in squamous cell carcinoma of head and neck(SCCHN) with in vitro approaches due to limited sample volumes. In this study, we applied the Cell Collector to capture CTC in vivo from peripheral blood.Methods: In total, the study included 22 cases with 37 times of detection. All of the patients were newly diagnosed with locally advanced or metastatic SCCHN, including laryngocarcinoma(40.9%, 9/22) and hypopharyngeal carcinoma(59.1%, 13/22). All patients received CTC analysis before treatment. Three patients received induction chemotherapy. Sixteen patients received surgical therapy, of which 13 patients received postoperative detection. Two patients received both induction chemotherapy and surgery treatment. Patients underwent two successive Cell Collector applications 24 h before and 7 d after surgical therapy. Nine healthy volunteers were enrolled as the control group. Epidermal growth factor receptor variant type Ⅲ(EGFRⅧ)expression was analyzed with fluorescent dye labeled antibody.Results: With Cell Collector isolation, 72.7%(16/22) of the patients were positive for ≥1 CTC(CTC; range,1–17 cells) before treatments and 46.7%(7/15) of patients were CTC positive for ≥1 CTC(CTC; range, 1–29 cells) after surgical therapy. Moreover, the detection rate of Cell Collector(82.4%, 14/17; CTC count range, 0–17)in advanced SCCHN(stage Ⅲ–Ⅳ) was much higher than that in early stages(stage Ⅰ–Ⅱ, 40.0%, 2/5; CTC count range, 0–2)(P<0.05). EGFRⅧ expression of CTC was also analyzed with fluorescence staining. One CTCEGFRⅧ-positive patient was detected from six CTC-positive patients, and the positive expression of EGFRⅧ was also found in the tumor tissue of this patient.Conclusions: In vivo detection of CTCs had high sensitivity in SCCHN, which might improve CTC application in clinic. | Haidong Zhang Shanchun Gong Yaqun Liu Longjun Liang Shuangba He Qingxiang Zhang Mingyuan Si Zhenkun Yu | 2017 | Chinese Journal of Cancer Research2017，29，3： | 1 |
| 9 | Shipwreck statistical analysis and suggestions for ships carrying liquefiable solid bulk cargoes in China显示文摘 | Cheng Xiaonan Liu Minyan He Longjun | 2014 | Procedia Engineering2014，，84： | 1 |
| 10 | Follow-Up of High-Intensity Focused Ultrasound Treatment for Patients with Hepatocellular Carcinoma显示文摘 | Guoliang Xu Guangyu Luo Longjun He Jianjun Li Hongbo Shan Rong Zhang Yin Li Xiaoyan Gao Shiyong Lin Guobao Wang | 2011 | Ultrasound in Medicine & Biology2011，，12： | 1 |
| 11 | Chlorophyllide-a Oxygenase 1(OsCAO1) Over-Expression Affects Rice Photosynthetic Rate and Grain Yield显示文摘Leaf color and photosynthesis are important factors for rice growth and development.Hence,improving the photosynthetic rate is an effective approach for increasing rice yield.We isolated a gene,chlorophyllide-a oxygenase 1(OsCAO1),which characterized a rice near-isogenic line named fgl(faded green leaf). | HU Ping MA Jie KANG Shujing LI Sanfeng WU Xianmei ZENG Longjun LU Caolin HE Rui HE Huiying SHANG Lianguang RAO Yuchun ZHU Xudong XIONG Guosheng QIAN Qian GUO Longbiao WANG Yuexing | 2023 | Rice science2023，30，2： | 1 |
| 12 | Amphibian-derived peptide homodimer OA-GL17d promotes skin wound regeneration through the miR-663a/TGF-β1/Smad axis显示文摘Background:Amphibian-derived peptides exhibit considerable potential in the discovery and development of new therapeutic interventions for clinically challenging chronic skin wounds.MicroRNAs(miRNAs)are also considered promising targets for the development of effective therapies against skin wounds.However,further research in this field is anticipated.This study aims to identify and provide a new peptide drug candidate,as well as to explore the underlying miRNA mechanisms and possible miRNA drug target for skin wound healing.Methods:A combination of Edman degradation,mass spectrometry and cDNA cloning were adopted to determine the amino acid sequence of a peptide thatwas fractionated from the secretion of Odorrana andersonii frog skin using gel-filtration and reversed-phase high-performance liquid chromatography.The toxicity of the peptide was evaluated by Calcein-AM/propidium iodide(PI)double staining against human keratinocytes(HaCaT cells),hemolytic activity against mice blood cells and acute toxicity against mice.The stability of the peptide in plasma was also evaluated.The prohealing potency of the peptide was determined by MTS,scratch healing and a Transwell experiment against HaCaT cells,full-thickness injury wounds and scald wounds in the dorsal skin of mice.miRNA transcriptome sequencing analysis,enzyme-linked immunosorbent assay,real-time polymerase chain reaction and western blotting were performed to explore the molecular mechanisms.Results:A novel peptide homodimer(named OA-GL17d)that contains a disulfide bond between the 16th cysteine residue of the peptide monomer and the sequence‘GLFKWHPRCGEEQSMWT’was identified.Analysis showed that OA-GL17d exhibited no hemolytic activity or acute toxicity,but effectively promoted keratinocyte proliferation and migration and strongly stimulated the repair of full-thickness injury wounds and scald wounds in the dorsal skin of mice.Mechanistically,OA-GL17d decreased the level of miR-663a to increase the level of transforming growth factor-β1(TGF-β1)and activate the subsequent TGF-β1/Smad signaling pathway,thereby resulting in accelerated skin wound re-epithelialization and granular tissue formation.Conclusions:Our results suggest that OA-GL17d is a new peptide drug candidate for skin wound repair.This study emphasizes the importance of exogenous peptides as molecular probes for exploring competing endogenous RNA mechanisms and indicates that miR-663a may be an effective target for promoting skin repair. | Yue Zhang Ying Wang Lin Zeng Yixiang Liu Huiling Sun Shanshan Li Siyu Wang Longjun Shu Naixin Liu Saige Yin Junsong Wang Dan Ni Yutong Wu Ying Yang Li He Buliang Meng Xinwang Yang | 2022 | Burns & Trauma2022，10，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员