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95篇 您的检索式:作者名="Haydock"
    题名 作者 年代 出处 被引量
1Molecular diagnostics in a teacup:Non-Instrumented Nucleic Acid Amplification(NINA) for rapid,low cost detection of Salmonella enterica显示文摘We report on the use of a novel non-instrumented platform to enable a Loop Mediated isothermal Amplification(LAMP) based assay for Salmonella enterica.Heat energy is provided by addition of a small amount(<150 g) of boiling water,and the reaction temperature is regulated by storing latent energy at the melting temperature of a lipid-based engineered phase change material.Endpoint classification of the reaction is achieved without opening the reaction tube by observing the fluorescence of sequence-specific FRET-based assimilating probes with a simple handheld fluorometer.At or above 22℃ ambient temperature the non-instrumented devices could maintain reactions above a threshold temperature of 61℃ for over 90 min-significantly longer than the 60 min reaction time.Using the simple format,detection limits were less than 20 genome copies for reactions run at ambient temperatures ranging from 8 to 36℃.When used with a pre-enrichment step and non-instrumented DNA extraction device,trace contaminations of Salmonella in milk close to 1 CFU/mL could be reliably detected.These findings illustrate that the non-instrumented amplification approach is a simple,viable,low-cost alternative for field-based food and agricultural diagnostics or clinical applications in developing countries.KUBOTA Ryo LABARRE Paul WEIGL Bernhard H LI Yong HAYDOCK Paul JENKINS Daniel M 2013Chinese Science Bulletin2013,58,10:4
2Fluid Therapy in Acute Pancreatitis: Anybody?s Guess显示文摘Matthew D. Haydock Anubhav Mittal Heath R. Wilms Anthony Phillips Maxim S. Petrov John A. Windsor 2013Annals of Surgery2013,,2:2
3Radiotoxicity of 75Se and 35S:theory and application to a cellular model显示文摘Kassis AI Adelstein SJ Haydock C 0,,03:1
4Management of dysfunction in the transplanted lung: experience with 7 clinical cases 显示文摘Haydock DA Trulock EP Kaiser LR 1992Ann Thorae Surg1992,53,2:1
5An unusually large multifunctional polypeptide in the erythromycin- producing polyketide synthase of Saecharopolyspora erythraea显示文摘Cortes J Haydock S F Roberts G A 1990Nature1990,348,:1
6An unusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythraea 显示文摘Cortes J Haydock S F Roberts G A 1990Nature1990,348,6297:1
7Effects of a serotonin 5-HT ( 4 ) receptor antagonist SB-207266 on gastrointestinal motor and sensory function in humans显示文摘Bharucha AE Camilleri M Haydock S 2000Gut2000,47,5:1
8Some observations on using meta-heuristics for efficient location management in mobile computing networks显示文摘Albert Y Zomaya Michael Haydock Stephan Olariu 2003Journal of Parallel and Distributed Computing2003,63,1:1
9Fluid therapy in acute pancreatitis: anybody's guess显示文摘Haydock MD Mittal A Wilms HR 2013Ann Surg2013,257,2:1
10The biosynthetic gene cluster for the polyketide immunosuppressant rapamycin 显示文摘Schwecke T Khsw L E Haydock S F 1995Natl Acad1995,92,:1
11Electronic structure based on local atomic environment for tight-binding bands: Ⅱ 显示文摘Haydock R Heine V Kelly M J 1975J Phys Solid Sate Phys1975,8,:1
12The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233: characterisafion of an aminotransferase involved in the formation of 2-deoxystreptamine 显示文摘HUANG F HAYDOCK SF MIRONENKO T 2005Org Biomol Chem2005,3,8:1
13Arotic valve replacement for active infectious endocarditis in 108 patients显示文摘Haydock D Barratt Boyes B Macedo T 1992J Thorac Cardiovasc Surg1992,103,1:1
14An anusually large multifunctional polypeptide in the erythromycin-producing polyketide synthase of Saccharopolyspora erythraea显示文摘Cortes J Haydock SF Roberts CA 1990Nature1990,348,6297:1
15Management of dysfunction in the transplanted lung:experience with 7 clinical cases显示文摘 Trulock EP Kaiser LR 1992Ann Thoroc Surg1992,53,:1
16Organisation of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: Analysis of genes flanking the polyketide synthase显示文摘Molnr I Aparicio J F Haydock S F 1996Gene1996,169,1:1
17Modelling and Simulation of Avariable Speed Standalone Generator System显示文摘Haydock L 2000Proceeding of lEE Conference on the Power Electronics and Variable Speed Drives2000,,:1
18Organisation of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: analysis of genes flanking the polyketide synthase显示文摘Molnar I Aparicio JF Haydock SF 1996Gene1996,169,1:1
19The neomycin biosynthetic gene cluster of Streptomyees fradiae NCIMB 8233: characterisation of an aminotransferase involvedin the formation of 2-deoxystreptamine显示文摘Huang F L Haydock S F Mironenko T 2005Org Biomol Chem2005,3,:1
20The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233: characterisation of an aminotransferase involved in the formation of 2-deoxystreptamine 显示文摘Huang F Haydock S F Mironenko T 2005Org Biomol Chem2005,3,8:1
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