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| 1 | Exosomal S100A4 derived from highly metastatic hepatocellular carcinoma cells promotes metastasis by activating STAT3显示文摘Intercellular cross-talk plays important roles in cancer progression and metastasis.Yet how these cancer cells interact with each other is still largely unknown.Exosomes released by tumor cells have been proved to be effective cell-to-cell signal mediators.We explored the functional roles of exosomes in metastasis and the potential prognostic values for hepatocellular carcinoma(HCC).Exosomes were extracted from HCC cells of different metastatic potentials.The metastatic effects of exosomes derived from highly metastatic HCC cells(HMH)were evaluated both in vitro and in vivo.Exosomal proteins were identified with iTRAQ mass spectrum and verified in cell lines,xenograft tumor samples,and functional analyses.Exosomes released by HMH significantly enhanced the in vitro invasion and in vivo metastasis of low metastatic HCC cells(LMH).S100 calcium-binding protein A4(S100A4)was identified as a functional factor in exosomes derived from HMH.S100A4r,ch exosomes significantly promoted tumor metastasis both in vitro and in vivo compared with S100A4^(rich) exosomes or controls.Moreover,exosomal S100A4 could induce expression of osteopontin(OPN),along with other tumor metastasis/stemness-related genes.Exosomal S100A4 activated OPN transcription via STAT3 phosphorylation.HCC patients with high exosomal S100A4 in plasma also had a poorer prognosis.In conclusion,exosomes from HMH could promote the metastatic potential of LMH,and exosomal S100A4 is a key enhancer for HCC metastasis,activating STAT3 phosphorylation and up-regulating OPN expression.This suggested exosomal S100A4 to be a novel prognostic marker and therapeutic target for HCC metastasis. | Haoting Sun Chaoqun Wang Beiyuan Hu Xiaomei Gao Tiantian Zou Qin Luo Mo Chen Yan Fu Yuanyuan Sheng Kaili Zhang Yan Zheng Xudong Ren Shican Yan Yan Geng Luyu Yang Qiongzhu Dong Lunxiu Qin | 2021 | Signal Transduction and Targeted Therapy2021，6，6： | 1 |
| 2 | Fully Developed Turbulence of Power⁃Law Fluids in Circular Pipe Based on Large Eddy Simulation显示文摘In this study,Large eddy simulation(LES)of the fully developed turbulence of power⁃law fluids in a circular pipe was performed using the dynamic subgrid⁃scale model.Under a specific Reynolds number,the flow information of three fluids with a range of power⁃law indexes was obtained.The trends of the mean axial velocity and the normalized apparent viscosity were analyzed.Simulation results show that shearing⁃thinning fluid displayed more noticeable non⁃Newtonian characteristics than shear⁃thickening fluid.The predicted friction factors were approximately equal to the Dodge and Metzner correlation and Gomes correlation.The peak values of root mean squares(RMS)and Reynolds stress increased as the power⁃law index increased.The turbulence statistics(skewness and flatness)from the wall to the pipe center were calculated.From the calculated results,the velocity fluctuation near the wall had strong intermittent and asymmetry.As demonstrated by the contours of the normalized instantaneous axial velocity and viscosity,the turbulence was more developed as the power⁃law index increased.It is concluded that the LES is feasible to predict the turbulence of pipe flow under higher Reynolds numbers. | Shuyan Wang Miao Li Shuqing Wang Haoting Li Yujia Chen Yimei Ma Qiji Sun | 2021 | Journal of Harbin Institute of Technology(New Series)2021，28，4： | 0 |
| 3 | Osteopontin targeted theranostic nanoprobes for laser-induced synergistic regression of vulnerable atherosclerotic plaques显示文摘Vulnerable atherosclerotic plaque(VASPs)is the major pathological cause of acute cardiovascular event.Early detection and precise intervention of VASP hold great clinical significance,yet remain a major challenge.Photodynamic therapy(PDT)realizes potent ablation efficacy under precise manipulation of laser irradiation.In this study,we constructed theranostic nanoprobes(NPs),which could precisely regress VASPs through a cascade of synergistic events triggered by local irradiation of lasers under the guidance of fluorescence/MR imaging.The NPs were formulated from human serum albumin(HSA)conjugated with a high affinity-peptide targeting osteopontin(OPN)and encapsulated with photosensitizer IR780 and hypoxia-activatable tirapazamine(TPZ).After intravenous injection into atherosclerotic mice,the OPN-targeted NPs demonstrated high specific accumulation in VASPs due to the overexpression of OPN in activated foamy macrophages in the carotid artery.Under the visible guidance of fluorescence and MR dual-model imaging,the precise near-infrared(NIR)laser irradiation generated massive reactive oxygen species(ROS),which resulted in efficient plaque ablation and amplified hypoxia within VASPs.In response to the elevated hypoxia,the initially inactive TPZ was successively boosted to present potent biological suppression of foamy macrophages.After therapeutic administration of the NPs for 2 weeks,the plaque area and the degree of carotid artery stenosis were markedly reduced.Furthermore,the formulated NPs displayed excellent biocompatibility.In conclusion,the developed HSA-based NPs demonstrated appreciable specific identification ability of VASPs and realized precise synergistic regression of atherosclerosis. | Mengqi Xu Cong Mao Haoting Chen Lu Liu Yabin Wang Abid Hussain Sulei Li Xu Zhang Ruslan G.Tuguntaev Xing-Jie Liang Weisheng Guo Feng Cao | 2022 | Acta Pharmaceutica Sinica B2022，12，4： | 0 |
| 4 | A review of solid-state lithium metal batteries through in-situ solidification显示文摘High-energy-density lithium metal batteries are the next-generation battery systems of choice,and replacing the flammable liquid electrolyte with a polymer solid-state electrolyte is a prominent conduct towards realizing the goal of high-safety and high-specific-energy devices.Unfortunately,the inherent intractable problems of poor solid-solid contacts between the electrode/electrolyte and the growth of Li dendrites hinder their practical applications.The in-situ solidification has demonstrated a variety of advantages in the application of polymer electrolytes and artificial interphase,including the design of integrated polymer electrolytes and asymmetric polymer electrolytes to enhance the compatibility of solid–solid contact and compatibility between various electrolytes,and the construction of artificial interphase between the Li anode and cathode to suppress the formation of Li dendrites and to enhance the high-voltage stability of polymer electrolytes.This review firstly elaborates the history of in-situ solidification for solid-state batteries,and then focuses on the synthetic methods of solidified electrolytes.Furthermore,the recent progress of in-situ solidification technology from both the design of polymer electrolytes and the construction of artificial interphase is summarized,and the importance of in-situ solidification technology in enhancing safety is emphasized.Finally,prospects,emerging challenges,and practical applications of in-situ solidification are envisioned. | Pan Xu Zong-Yao Shuang Chen-Zi Zhao Xue Li Li-Zhen Fan Aibing Chen Haoting Chen Elena Kuzmina Elena Karaseva Vladimir Kolosnitsyn Xiaoyuan Zeng Peng Dong Yingjie Zhang Mingpei Wang Qiang Zhang | 2024 | Science China Chemistry2024，67，1： | 0 |
| 5 | Inactivated SARS-CoV-2 booster vaccine enhanced immune responses in patients with chronic liver diseases显示文摘Chronic liver disease(CLD)entails elevated risk of COVID-19 severity and mortality.The effectiveness of the booster dose of inactivated SARS-CoV-2 vaccine in stimulating antibody response in CLD patients is unclear.Therefore,we conducted a cross-sectional study involving 237 adult CLD patients and 170 healthy controls(HC)to analyze neutralizing antibodies(NAbs)against SARS-CoV-2 prototype and BA.4/5 variant,anti-receptor binding domain(RBD)IgG,and total anti-SARS-CoV-2 antibodies.Serum levels of the total anti-SARS-CoV-2 antibodies,anti-RBD IgG and inhibition efficacy of NAbs were significantly elevated in CLD patients after the booster dose compared with the pre-booster dose,but were relatively lower than those of HCs.Induced humoral responses decreased over time after booster vaccination.The neutralization efficiency of the serum against BA.4/5 increased but remained below the inhibition threshold.All four SARS-CoV-2 antibodies,including total anti-SARS-CoV-2 antibodies,anti-RBD IgG and NAbs against prototype and BA.4/5,were lower in patients with severe CLD than those with non-severe CLD.After booster shot,age and time after the last vaccine were the risk factors for seropositivity of NAb against BA.4/5 in CLD patients.Additionally,white blood cell counts and hepatitis B core antibodies were the protective factors,and severe liver disease was the risk factor associated with seropositivity of total anti-SARS-CoV-2 antibodies.Overall,our data uncovered that antibody responses were improved in CLD patients and peaked at 120 days after the booster vaccines.All antibodies excepting total anti-SARS-CoV-2 antibodies declined after peak.CLD patients exhibited impaired immunologic responses to vaccination and weakened NAbs against BA.4/5,which hindered the protective effect of the booster shot against Omicron prevalence.Cellular immune responses should be further evaluated to determine the optimal vaccine regimen for CLD patients. | Yongmei Liu Jianhua Lu Haoting Zhan Wenfang Yuan Xiaomeng Li Haiyan Kang Haolong Li Yongliang Chen Linlin Cheng Xingli Sun Haojie Zheng Wei Wang Erhei Dai Yongzhe Li | 2023 | Virologica Sinica2023，38，5： | 0 |
| 6 | Stimuli-responsive polymeric nanomaterials for rheumatoid arthritis therapy显示文摘Rheumatoid arthritis(RA)is a long-term inflammatory disease derived from an autoimmune disorder of the synovial membrane.Current therapeutic strategies for RA mainly aim to hamper the macrophages’proliferation and reduce the production of pro-inflammatory cytokines.Therefore,the accumulation of therapeutic agents targeted at the inflammatory site should be a crucial therapeutic strategy.Nowadays,the nanocarrier system incorporated with stimuli-responsive property is being intensively studied,showing the potentially tremendous value of specific therapy.Stimuli-responsive(i.e.,pH,temperature,light,redox,and enzyme)polymeric nanomaterials,as an important component of nanoparticulate carriers,have been intensively developed for various diseases treatment.A survey of the literature suggests that the use of targeted nanocarriers to deliver therapeutic agents(nanotherapeutics)in the treatment of inflammatory arthritis remains largely unexplored.The lack of suitable stimuli-sensitive polymeric nanomaterials is one of the limitations.Herein,we provide an overview of drug delivery systems prepared from commonly used stimuli-sensitive polymeric nanomaterials and some inorganic agents that have potential in the treatment of RA.The current situation and challenges are also discussed to stimulate a novel thinking about the development of nanomedicine. | Yingsi Xie Ruslan G.Tuguntaev Cong Mao Haoting Chen Ying Tao Shixiang Wang Bin Yang Weisheng Guo | 2020 | Biophysics Reports2020，6，5： | 0 |
| 7 | Inflammation responsive tofacitinib loaded albumin nanomedicine for targeted synergistic therapy in ulcerative colitis显示文摘Patients with ulcerative colitis(UC)often loss responses over long term usage of conventional therapies.Tofacitinib,a pan-Janus kinases(JAK)inhibitor is approved for moderate to severe UC treatment,while dose-limiting systemic side effects including infections,cancers and lymphoma limit its popularity of clinical application.This study sought to construct an anti-mucosal vascular addressin cell-adhesion molecule-1(anti-MAdCAM-1)antibody modified reactive oxygen species(ROS)responsive human serum albumin-based nanomedicine denoted as THM,to improve the therapeutic efficacy of tofacitinib for UC treatment.THM has the drug releasing properties in response to ROS stimulation.In vitro studies show that THM selectively adhered to the endothelial cells and had obvious anti-inflammatory effect on macrophages.Meanwhile,the nanomedicine can inhibit the phenotypic switching of M1 macrophages and promote M2 polarization to produce anti-inflammatory medicators during wound healing.In addition,in vivo fluorescence imaging verified that THM exhibited enhanced preferential accumulation and extended retention in inflamed colon.Moreover,THM significantly reduced the production of proinflammatory cytokines in the colon and suppressed the homing of T cells to the gut in dextran sodium sulfate induced experimental colitis.This work elucidates that the inflamed colon-targeted delivery of tofacitinib by nanomedicine is promising for UC treatment and sheds light on addressing the unmet medical need. | Bang Li Xiaoyan Liu Qi Long Xiaoduan Zhuang Yanfei Gao Barkat Ali Haoting Chen Dongyang Zhang Xinying Wang Weisheng Guo | 2023 | Nano Research2023，16，7： | 0 |
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