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| 1 | A complete sequence and comparative analysis of a SARS-associated virus(Isolate BJ01)显示文摘The genome sequence of the Severe Acute Respiratory Syndrome (SARS)-associated virus provides essential information for the identification of pathogen(s), exploration of etiology and evolution, interpretation of transmission and pathogenesis, development of diagnostics, prevention by future vaccination, and treatment by developing new drugs. We report the complete genome sequence and comparative analysis of an isolate (BJ01) of the coronavirus that has been recognized as a pathogen for SARS. The genome is 29725 nt in size and has 11 ORFs (Open Reading Frames). It is composed of a stable region encoding an RNA-dependent RNA polymerase (composed of 2 ORFs) and a variable region representing 4 CDSs (coding sequences) for viral structural genes (the S, E, M, N proteins) and 5 PUPs (putative uncharacterized proteins). Its gene order is identical to that of other known coronaviruses. The sequence alignment with all known RNA viruses places this virus as a member in the family of Coronaviridae. Thirty putative substitutions have been identified by comparative analysis of the 5 SARS- associated virus genome sequences in GenBank. Fifteen of them lead to possible amino acid changes (non-synonymous mutations) in the proteins. Three amino acid changes, with predicted alteration of physical and chemical features, have been detected in the S protein that is postulated to beinvolved in the immunoreactions between the virus and its host. Two amino acid changes have been detected in the Mprotein, which could be related to viral envelope formation. Phylogenetic analysis suggests the possibility of non-human origin of the SARS-associated viruses but provides noevidence that they are man-made. Further efforts should focus on identifying the etiology of the SARS-associated virus and ruling out conclusively the existence of otherpossible SARS-related pathogen(s). | QIN E'de ZHU Qingyu YU Man FAN Baochang CHANG Guohui SI Bingyin YANG Bao PENG Wenming JIANG Tao LIU Bohua DENG Yongqiang LIU Hong ZHANG Yu WANG Cui LI Yuquan GAN Yonghua LI Xiaoyu L Fushuang TAN Gang CAO Wuchun, YANG Ruifu Institute of Microbiology and Epidemiology, Chinese Academy of Military Medical Sciences, Beijing 100071, China WANG Jian, LI Wei, XU Zuyuan, LI Yan, WU Qingfa, LIN Wei, CHEN Weijun, TANG Lin, DENG Yajun, HAN Yujun, LI Changfeng, LEI Meng, LI Guoqing, LI Wenjie, L Hong, SHI Jianping, TONG Zongzhong, ZHANG Feng, LI Songgang, LIU Bin, LIU Siqi, DONG Wei, WANG Jun, Gane K-S Wong, YU Jun & YANG Huanming* Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300 National Center for Genome Information, Beijing 101300, China | 2003 | Chinese Science Bulletin2003，48，10： | 121 |
| 2 | Prevalence and evolution of drug resistance HIV-1 variants in Henan, China显示文摘To understand the prevalence and evolution of drug resistant HIV strains in Henan China after the implementation of free antiretroviral therapy for AIDS patients. 45 drug nave AIDS patients, 118 AIDS patients who received three months antiretroviral therapy and 124 AIDS patients who received six months antiretroviral treatment were recruited in the southern part of Henan province. Information on general condition, antiretroviral medicines, adherence and clinical syndromes were collected by face to face interview. Meanwhile, 14ml EDTA anticoagulant blood was drawn. CD4/CD8 T cell count, viral load and genotypic drug resistance were tested. The rates of clinical improvement were 55.1% and 50.8% respectively three months and six months after antiretroviral therapy. The mean CD4 cell count after antiretroviral therapy was significantly higher than in drug nave patients. The prevalence rate of drug resistant HIV strains were 13. 9%, 45.4% and 62.7% in drug nave patients, three month treatment patients and six month treatment patients, respectively. The number of resistance mutation codons and the frequency of mutations increased significantly with continued antiretroviral therapy. The mutation sites were primarily at the 103, 106 and 215 codons in the three-month treatment group and they increased to 15 codon mutations in the six-month treatment group. From this result, the evolution of drug resistant strains was inferred to begin with the high level NNRTI resistant strain, and then develop low level resistant strains to NRTIs. The HIV strains with high level resistance to NVP and low level resistance to AZT and DDI were highly prevalent because of the AZT+DDI+NVP combination therapy. These HIV strains were also cross resistant to DLV, EFV, DDC and D4T. Poor adherence to therapy was believed to be the main reason for the emergence and prevalence of drug resistant HIV strains. The prevalence of drug resistant HIV strains was increased with the continu- ation of antiretroviral therapy in the southern part of Henan province. Measures, that could promote high level adherence, provide new drugs and change ART regimens in failing patients, should be implemented as soon as possible. | Jing Yun LI Han Ping LI Lin LI Hong LI Zhe WANG Kun YANG Zuo Yi BAO Dao Min ZHUANG Si Yang LIU Yong Jian LIU Hui XING Yi Ming SHAO | 2005 | Cell Research2005，15，11： | 34 |
| 3 | Induction of the LRP16 gene by estrogen promotes the invasive growth of Ishikawa human endometrial cancer cells through the downregulation of E-cadherin显示文摘LRP16 以前在乳癌房间作为导致雌激素的基因被识别。到在子宫内膜的癌症(EC ) 的雌激素和它的功能的效果的 LRP16 的应答的海角房间仍然是不清楚的。这里,我们证明 LRP16 基因的信使 rna 水平和倡导者活动被 17beta-estradiol (E2 ) 显著地在雌激素受体高山增加哈(嗯高山哈)-positiveIshikawa 人 EC 房间。尽管 Ishikawa 细胞的生长率没被 LRP16 的宫外的表示显然影响, Transwell 试金的结果显示出 LRP16-overexpressing 细胞的侵略能力的近似 one-thirdincrease。由于分子的屏蔽,我们观察到 E-cadherin 的表示,与肿瘤转移联系的一个必要粘附分子,被 LRP16 镇压。进一步的倡导者分析以一种剂量依赖者方式表明了那 LRP16 inhibitedE-cadherin transactivation。然而,抑制被雌激素剥夺废除,显示由 LRP16requires 的 E-cadherin 抄写的 down 规定嗯高山哈调停。染色质免疫降水分析表明到 E-cadherin 倡导者的 ERalpha 的绑定被 LRP16 反对,建议那 LRP16 能防碍嗯调停 alpha 的抄写。这些结果建议起来由雌激素的 LRP16 的规定能被在人的 EC 调整 E-cadherin 的 down 涉及侵略生长。 | Yuan Guang Meng Wei Dong Han Ya Li Zhao Ke Huang Yi Ling Si Zhi Qiang Wu Yi Ming Mu | 2007 | Cell Research2007，17，10： | 27 |
| 4 | Benefit of neoadjuvant concurrent chemoradiotherapy for locally advanced perihilar cholangiocarcinoma显示文摘AIM To clarify the role of neoadjuvant concurrent chemoradiotherapy(NACCRT) followed by surgical resection for localized or locally advanced perihilar cholangiocarcinoma(CCA).METHODS We retrospectively reviewed 57 patients who underwent surgical resection with or without NACCRT for perihilar CCA; 12 patients received NACCRT and 45 patients did not received NACCRT. Patients with locally advanced perihilar CCA requiring NACCRT were defined as follows:(1) a mass involving unilateral branches of the portal vein or hepatic artery with insufficient volume of the anticipated remnant lobe; or(2) an infiltrating mass in the main portal vein that was too long for reconstruction, identified at preoperative staging. RESULTS The median disease-free survival(DFS) durations of the neoadjuvant and non-neoadjuvant CCRT groups were26.0 and 15.1 mo, respectively(P = 0.91). The median overall survival(OS) durations of the neoadjuvant and non-neoadjuvant CCRT groups were 32.9 and 27.1 mo, respectively(P = 0.26). The NACCRT group showed a downstaging tendency compared to the non-NACCRT group as compared with the tumor stage confirmed by histological examination after surgery and the tumor stage confirmed by imaging test at the time of diagnosis(P = 0.01). CONCLUSION NACCRT does not prolong DFS and OS in localized or locally advanced perihilar CCA. However, NACCRT may allow tumor downstaging and improve tumor resectability. | Jang Han Jung Hyun Jik Lee Hee Seung Lee Jung Hyun Jo In Rae Cho Moon Jae Chung Jeong Youp Park Seung Woo Park Si Young Song Seungmin Bang | 2017 | World Journal of Gastroenterology2017，23，18： | 21 |
| 5 | GPER agonist G1 suppresses neuronal apoptosis mediated by endoplasmic reticulum stress after cerebral ischemia/reperfusion injury显示文摘Studies have confirmed a strong association between activation of the endoplasmic reticulum stress pathway and cerebral ischemia/reperfusion(I/R) injury.In this study,three key proteins in the endoplasmic reticulum stress pathway(glucose-regulated protein 78,caspase-12,and C/EBP homologous protein) were selected to examine the potential mechanism of endoplasmic reticulum stress in the neuroprotective effect of G protein-coupled estrogen receptor.Female Sprague-Dawley rats received ovariectomy(OVX),and then cerebral I/R rat models(OVX+ I/R) were established by middle cerebral artery occlusion.Immediately after I/R,rat models were injected with 100 μg/kg E2(OVX + I/R +E2),or 100 μg/kg G protein-coupled estrogen receptor agonist G1(OVX + I/R + G1) in the lateral ventricle.Longa scoring was used to detect neurobehavioral changes in each group.Infarct volumes were measured by 2,3,5-triphenyltetrazolium chloride staining.Morphological changes in neurons were observed by Nissl staining.Terminal dexynucleotidyl transferase-mediated nick end-labeling staining revealed that compared with the OVX + I/R group,neurological function was remarkably improved,infarct volume was reduced,number of normal Nissl bodies was dramatically increased,and number of apoptotic neurons in the hippocampus was decreased after E2 and G1 intervention.To detect the expression and distribution of endoplasmic reticulum stress-related proteins in the endoplasmic reticulum,caspase-12 distribution and expression were detected by immunofluorescence,and mRNA and protein levels of glucose-regulated protein 78,caspase-12,and C/EBP homologous protein were determined by polymerase chain reaction and western blot assay.The results showed that compared with the OVX+ I/R group,E2 and G1 treatment obviously decreased mRNA and protein expression levels of glucose-regulated protein 78,C/EBP homologous protein,and caspase-12.However,the G protein-coupled estrogen receptor antagonist G15(OVX + I/R + E2 + G15) could eliminate the effect of E2 on cerebral I/R injury.These results confirm that E2 and G protein-coupled estrogen receptor can inhibit the expression of endoplasmic reticulum stress-related proteins and neuronal apoptosis in the hippocampus,thereby improving dysfunction caused by cerebral I/R injury.Every experimental protocol was approved by the Institutional Ethics Review Board at the First Affiliated Hospital of Shihezi University School of Medicine,China(approval No.SHZ A2017-171) on February 27,2017. | Zi-Wei Han Yue-Chen Chang Ying Zhou Hang Zhang Long Chen Yang Zhang Jun-Qiang Si Li Li | 2019 | Neural Regeneration Research2019，14，7： | 16 |
| 6 | Double role of Fas ligand in the apoptosis of intervertebral disc cells in vitro显示文摘船边交货 ligand (FasL ) 可以在维持 intervertebral 圆盘(IVD ) 的有免疫力的特权起一个重要作用。而且,它是仔细与退化圆盘房间的 apoptosis 有关。现在,大量报告关于 FasL 的矛盾的效果描述了,尽管 IVD 房间上的 FasL 的效果仍然在争论下面。在这研究,我们试着在船边交货表示上并且在原子核 pulposus (NP ) 的 apoptosis 上调查 FasL 的效果在 SpragueDawley 老鼠的房间。结果证明在 NP 房间的船边交货的表示被 recombinant FasL 显著地增加。同时, NP 房间的 apoptosis 以一种 FasL 剂量依赖者方式显著地增加了。有趣地, RNA 干扰结果显示船边交货表示和以前描述的 NP 房间 apoptosis 的增加被船边交货 siRNA 禁止,建议 RNA 干扰可能是阻止 IVD 房间 apoptosis 的新奇策略之一。 | Dunfu Han Yue Ding Shang-Li Liu Gang Wang In-Cheong Si Xinguang Wang Liyang Cui Dongshang Huang | 2009 | Acta Biochimica et Biophysica Sinica2009，41，11： | 14 |
| 7 | Eleven COVID-19 Outbreaks with Local Transmissions Caused by the Imported SARS-CoV-2 Delta VOC-China,July-August,2021显示文摘Starting from December 2019,Wuhan,China,encountered the first outbreak of coronavirus disease 2019(COVID-19)(1-2).The epidemic was successfully suppressed by strict containment so that the number of infected people was reduced to 0 on April 8,2020(3–4).After that,China experienced roughly 3 dozen outbreaks with local transmission caused by imported severe acute respiratory syndrome coronavirus 2(SARS-CoV-2). | Lei Zhou Kai Nie Hongting Zhao Xiang Zhao Bixiong Ye Ji Wang Cao Chen Hong Wang Jiangli Di Jinsong Li Chao Li Zhixiao Chen Ruiqi Ren Peihua Niu Hui Chen Tao Chen Yali Wang Lili Li Bingxue Han Ruhan A Si Chen Dan Li Dan Li Chunxia Jin Xin Zhang Jiangmei Liu Chunyu Xu Yecheng Yao Yenan Feng Zhili Li Bike Zhang Yang Song Peter Hao Yanping Zhang Hao Li Qun Li George F.Gao Guoqing Shi Wenbo Xu | 2021 | China CDC weekly2021，3，41： | 13 |
| 8 | Oil–water pre-separation with a novel axial hydrocyclone显示文摘A novel hydrocyclone with guide vanes, named as axial hydrocyclone(AHC), is designed to tackle the problem of oil–water separation faced by most mature oilfields. Optimal design of the AHC is carried out by using numerical methods. The effects of guide vanes, cone angle, tapered angle and overflow pipe on the oil–water separation are discussed in this paper. The results show that a double swirling flow is generated in the tapered section where oil–water separation occurs. Both the cylindrical and the tapered section have important influences on AHC performance. On the basis of single factor results, response surface methodology is employed to optimize the AHC design. The experimental results indicate that the novel AHC has an excellent performance for the oil–water separation. | Meili Liu Jiaqing Chen Xiaolei Cai Yanhe Han Si Xiong | 2018 | Chinese Journal of Chemical Engineering2018，26，1： | 12 |
| 9 | Primary intestinal lymphangiectasia diagnosed by capsule endoscopy and double balloon enteroscopy显示文摘Primary intestinal lymphangiectasia(PIL)is a rare disorder characterized by dilated intestinal lymphatics and the development of protein-losing enteropathy.Patients with PIL develop hypoalbuminemia,hypocalcemia,lymphopenia and hypogammaglobulinemia,and present with bilateral lower limb edema,fatigue,abdominal pain and diarrhea.Endoscopy reveals diffusely elongated,circumferential and polypoid mucosae covered with whitish enlarged villi,all of which indicate intestinal lymphangiectasia.Diagnosis is conf irmed by characteristic tissue pathology,which includes dilated intestinal lymphatics with diffusely swollen mucosa and enlarged villi.The prevalence of PIL has increased since the introduction of capsule endoscopy.The etiology and prevalence of PIL remain unknown.Some studies have reported that several genes and regulatory molecules for lymphangiogenesis are related to PIL.We report the case of a patient with PIL involving the entire small bowel that was confirmed by capsule endoscopy and double-balloon enteroscopy-guided tissue pathology who carried a deletion on chromosome 4q25.The relationship between this deletion on chromosome 4 and PIL remains to be investigated. | Tak Geun Oh Joo Won Chung Hee Man Kim Seok-Joo Han Jin Sung Lee Jung Yeob Park Si Young Song | 2011 | World Journal of Gastrointestinal Endoscopy2011，3，11： | 12 |
| 10 | Endogenously produced FGF2 is essential for the survival and proliferation of cultured mouse spermatogonial stem cells显示文摘 | Yan Zhang Si Wang Xiuxia Wang Shangying Liao Yujian Wu Chunsheng Han | 2012 | Cell Research2012，22，4： | 10 |
| 11 | Development of the personalized criteria for microscopic review following four different series of hematology analyzer in a Chinese large scale hospital显示文摘 | CUI Wei WU Wei WANG Xin WANG Geng HAO Ying-ying CHEN Yu LUO Dan SHOU Wei-ling ZHANG Shuo XIANG Xue-fang SI Yong-zhen CHEN Qian CAI Hao LI Tan SHEN Han SHANG Kun ZHANG Yong-qiang | 2010 | Chinese Medical Journal2010，，22： | 10 |
| 12 | Huangjing(Polygonati rhizoma)is an emerging crop with great potential to fight chronic and hidden hunger显示文摘According to an annual flagship report produced jointly by the FAO and several other organizations,almost 690 million people,or 8.9 percent of the world’s population,were still suffering from chronic hunger in 2019(FAO et al.,2020). | Donghong Chen Zhigang Han Jinping Si | 2021 | Science China(Life Sciences)2021，64，9： | 10 |
| 13 | Pediculate Brachiopod Diandongia pista from the Lower Cambrian of South China显示文摘The Chengjiang Lagerstatte has been celebrated for prolific soft-bodied fossils. Based on specimens recentlyexcavated in the Chengjiang Lagerstatte by the Early Life Institute, Northwest University, Diandongiapista Rong, 1974, isdirectly revealed to be a pediculate brachiopod, assigned to the Family Botsfordiidae, as is further confirmed by theexceptionally preserved vascular system including dorsal and ventral mantle canals. These specimens described hereinexhibit some peculiarities, notably the extremely thin and long pedicles, which suggest that Diandongia is epifaunal ratherthan burrowing brachiopod. A study of this group of animals indicates that they may be vital to understand the relationshipbetween the lingulids and the remaining brachiopods, and the character evolution of the early Cambrian brachiopods. | ZHANG Zhifei, HAN Jian, ZHANG Xingliang, LIU Jianni and SHU DeganEarly Life Institute and Key Laboratory for Continental Dynamics of the Ministry of Education,Northwest University, Xi’an, Shaanxi 710069 E-mail: zhangelle@sina.com.cn Wang Si’en and Xie Guanglian | 2003 | Acta Geologica Sinica(English Edition)2003，77，3： | 9 |
| 14 | Association between Helicobacter pylori status and metachronous gastric cancer after endoscopic resection显示文摘AIM To investigate the effect of Helicobacter pylori(H. pylori) status test and H. pylori eradication on the occurrence of metachronous gastric cancer(MGC) after endoscopic submucosal dissection(ESD) of early gastric cancer(EGC) and risk factors of MGC. METHODS The authors retrospectively reviewed the medical records of 433 patients(441 lesions) who underwent ESD for EGC from January 2005 to January 2015 in Yeungnam University Hospital. Patients were categorized into two groups; the H. pylori tested group(n = 257) and the H. pylori non-tested group(n = 176) based on performance of H. pylori status test after ESD of EGC. The H. pylori tested group was further categorized into three subgroups based on H. pylori status; the H. pylori-eradicated subgroup(n = 120), the H. pylori-persistent subgroup(n = 42), and the H. pylori-negative subgroup(n = 95). Incidences of MGC and risk factors of MGC were identified.RESULTS Median follow-up duration after ESD was 30.00 mo(range, 6-107 mo). Total 15 patients developed MGC during follow-up. MGC developed in 11 patients of the H. pylori tested group(7 in the H. pylori-negative subgroup, 3 in the H. pylori-eradicated subgroup, and 1 in the H. pylori-persistent subgroup) and 4 patients of the H. pylori non-tested group(P > 0.05). The risk factors of MGC were endoscopic mucosal atrophy in the H. pylori tested group and intestinal metaplasia in all patients. CONCLUSION H. pylori eradication and H. pylori status test seems to have no preventive effect on the development of MGC after ESD for EGC. The risk factors of MGC development were endoscopic mucosal atrophy in the H. pylori tested group alone and intestinal metaplasia in all patients. | Sung Bum Kim Si Hyung Lee Seung Il Bae Yo Han Jeong Se Hoon Sohn Kyeong Ok Kim Byung Ik Jang Tae Nyeun Kim | 2016 | World Journal of Gastroenterology2016，22，44： | 8 |
| 15 | The future of remote ECG monitoring systems显示文摘监视系统的遥远的 ECG 为遥远的心脏监测器正在成为平凡医药设备。在最近的年里,监视系统的遥远的 ECG 在监视心疾病的各种各样的类型被使用了,并且 ECG 的传播和接收的质量在保留的遥远的过程期间发信号进展。然而,在那里留下伴随挑战。这份报告集中于监视系统的遥远的 ECG 的三个部件:病人(终端用户),医生工作站,和遥远的服务器,在便携系统,在遥远的传播的包损失,便携式的 ECG 监视系统,耐心的 ECG 数据收集系统,和 ECG 上考察并且评估逼近的挑战包括即时处理表明传播圣片断, R 波浪, RR 间隔和 QRS 飘动,等等。这篇论文试着澄清未来 ECG 的发展策略遥远的监视,它能在指导监视的遥远的 ECG 的研究和发展是有用的。 | Shu-LI GUO Li-Na HAN Hong-Wei LIU Quan-Jin SI De-Feng KONG Fu-Su GUO | 2016 | Journal of Geriatric Cardiology2016，13，6： | 8 |
| 16 | Effect of yellow rice wine on anti-aging ability in aged mice induced by D-galactose显示文摘The present study is focused on investigation of the health protective effect of yellow rice wine(YRW)in mice and the potential underlying mechanism.Symptoms of aging were induced in mice by using Dgalactose(D-gal),and different doses of YRW(4,8,and 12 mL/kg BW)were orally administrated to D-galtreated mice for a period of six weeks.The in vivo data obtained in the present study preliminary indicated that YRW markedly increased the activity of antioxidant enzymes and reduced the concentration of MDA both in the brain and liver.Furthermore,a forced swim test showed that moderate intake of YRW(8 mL/kg BW)significantly decreased the duration of immobility,reduced the blood content of urea nitrogen and lactic acid,and increased hepatic glycogen storage.Besides,results from the Morris water maze test suggested that YRW significantly reversed cognitive impairment and also alleviated neuroapoptosis in the experimental mice by regulating the gene expressions of Bax/Bcl-2 and caspase-3.The above results indicated YRW has a potential anti-aging effect in mice and provided us certain molecular evidence for this action. | Rong Liu Zukang Fu Fengjie Zhang Qingzhong Mao Chunguang Luan Xinglin Han Jie Xue Deliang Wang Si Qin Feike Hao | 2020 | Food Science and Human Wellness2020，9，2： | 8 |
| 17 | 863 genomes reveal the origin and domestication of chicken显示文摘Despite the substantial role that chickens have played in human societies across the world,both the geographic and temporal origins of their domestication remain controversial.To address this issue,we analyzed 863 genomes from a worldwide sampling of chickens and representatives of all four species of wild jungle fowl and each of the five subspecies of red jungle fowl(RJF).Our study suggests that domestic chickens were initially derived from the RJF subspecies Gallus gallus spadiceus whose present-day distribution is predominantly in southwestern China,northern Thailand and Myanmar.Following their domestication,chickens were translocated across Southeast and South Asia where they interbred locally with both RJF subspecies and other jungle fowl species.In addition,our results show that the White Leghorn chicken breed possesses a mosaic of divergent ancestries inherited from other subspecies of RJF.Despite the strong episodic gene flow from geographically divergent lineages of jungle fowls,our analyses show that domestic chickens undergo genetic adaptations that underlie their unique behavioral,morphological and reproductive traits.Our study provides novel insights into the evolutionary history of domestic chickens and a valuable resource to facilitate ongoing genetic and functional investigations of the world's most numerous domestic animal. | Ming-Shan Wang Mukesh Thakur Min-Sheng Peng Yu Jiang Laurent Alain Francois Frantz Ming Li Jin-Jin Zhang Sheng Wang Joris Peters Newton Otieno Otecko Chatmongkon Suwannapoom Xing Guo Zhu-Qing Zheng Ali Esmailizadeh Nalini Yasoda Hirimuthugoda Hidayat Ashari Sri Suladari Moch Syamsul Arifin Zein Szilvia Kusza Saeed Sohrabi Hamed Kharrati-Koopaee Quan-Kuan Shen Lin Zeng Min-Min Yang Ya-Jiang Wu Xing-Yan Yang Xue-Mei Lu Xin-Zheng Jia Qing-Hua Nie Susan Joy Lamont Emiliano Lasagna Simone Ceccobelli Humpita Gamaralalage Thilini Nisanka Gunwardana Thilina Madusanka Senasige Shao-Hong Feng Jing-Fang Si Hao Zhang Jie-Qiong Jin Ming-Li Li Yan-Hu Liu Hong-Man Chen Cheng Ma Shan-Shan Dai Abul Kashem Fazlul Haque Bhuiyan Muhammad Sajjad Khan Gamamada Liyanage Lalanie Pradeepa Silva Thi-Thuy Le Okeyo Ally Mwai Mohamed Nawaz Mohamed Ibrahim Megan Supple Beth Shapiro Olivier Hanotte Guojie Zhang Greger Larson Jian-Lin Han Dong-Dong Wu Ya-Ping Zhang | 2020 | Cell Research2020，30，8： | 7 |
| 18 | FHL2 inhibits the Id3-promoted proliferation and invasive growth of human MCF-7 breast cancer cells显示文摘 | CHEN Yi-hong WU Zhi-qiang ZHAO Ya-li SI Yi-ling GUO Ming-zhou HAN Wei-dong | 2012 | Chinese Medical Journal2012，，13： | 6 |
| 19 | Effects of antiviral agents and HBV genotypes on intrahepaticcovalently closed circular DNA in HBeAg-positive chronichepatitis B patients显示文摘AIM:To evaluate the effects of antiviral agents and HBV genotypes on intrahepatic covalently closed circular DNA (ccc DNA) in HBeAg-positive chronic hepatitis B patients. METHODS:Seventy-one patients received lamivudine (n = 35), or sequential therapy with lamivudine-interferon alpha 2b (IFN-α 2b, n = 24) for 48 wk, or IFN-α 2b (n = 12) for 24 wk. All subjects were followed up for 24 wk. Intrahepatic ccc DNA was measured quantitatively by PCR. HBV genotypes were analyzed by PCR-RFLP. RESULTS:Sequential lamivudine-INF-α therapy, lamivudine and INF-α monotherapy reduced ccc DNA of 1.7 log, 1.4 log and 0.8 log, respectively (P < 0.05). Seventeen out of the 71 patients developed HBeAg seroconversion, the reduction of ccc DNA in the HBeAg seroconversion patients was more significant than that in the HBeAg positive patients (3.0 log vs 1.6 log, P = 0.0407). Twenty-four weeks after antiviral therapy withdrawal, 16 patients had a sustained virological response, the baseline intrahepatic ccc DNA in the patients with a sustained virological response was significantly lower than that in the patients with virological rebound (4.6 log vs 5.4 log, P = 0.0472). HBV genotype C accounted for 85.9% (n = 61), and genotype B for 14.1% (n = 10), respectively, in the 71 patients. There was no significant difference in the change of ccc DNA level between HBV genotypes C and B (2.1 log vs 1.9 log). CONCLUSION:Forty-eight week sequential lamivudine-INF-α therapy and lamivudine monotherapy reduce ccc DNA more significantly than 24-wk INF-α monotherapy. Low baseline intrahepatic ccc DNA level may predict the long-term efficacy of antiviral treatment. HBV genotypes C and B have no obvious influence on ccc DNA load. | Hai-Ying Lu Li-Wei Zhuang Yan-Yan Yu Chong-Wen Si Jun Li Jian-Jun Zhang Zheng Zeng Xin-Yue Chen Zhong-Hou Han Yong Chen | 2008 | World Journal of Gastroenterology2008，14，8： | 6 |
| 20 | Comparative genomic and transcriptomic analyses uncover the molecular basis of high nitrogen-use efficiency in the wheat cultivar Kenong 9204显示文摘Studying the regulatory mechanisms that drive nitrogen-use efficiency(NUE)in crops is important for sustainable agriculture and environmental protection.In this study,we generated a high-quality genome assembly for the high-NUE wheat cultivar Kenong 9204 and systematically analyzed genes related to nitrogen uptake and metabolism.By comparative analyses,we found that the high-affinity nitrate transporter gene family had expanded in Triticeae.Further studies showed that subsequent functional differentiation endowed the expanded family members with saline inducibility,providing a genetic basis for improving the adaptability of wheat to nitrogen deficiency in various habitats.To explore the genetic and molecular mechanisms of high NUE,we compared genomic and transcriptomic data from the high-NUE cultivar Kenong 9204(KN9204)and the low-NUE cultivar Jing 411 and quantified their nitrogen accumulation under high-and low-nitrogen conditions.Compared with Jing 411,KN9204 absorbed significantly more nitrogen at the reproductive stage after shooting and accumulated it in the shoots and seeds.Transcriptome data analysis revealed that nitrogen deficiency clearly suppressed the expression of genes related to cell division in the young spike of Jing 411,whereas this suppression of gene expression was much lower in KN9204.In addition,KN9204 maintained relatively high expression of NPF genes for a longer time than Jing 411 during seed maturity.Physiological and transcriptome data revealed that KN9204 was more tolerant of nitrogen deficiency than Jing 411,especially at the reproductive stage.The high NUE of KN9204 is an integrated effect controlled at different levels.Taken together,our data provide new insights into the molecular mechanisms of NUE and important gene resources for improving wheat cultivars with a higher NUE trait. | Xiaoli Shi Fa Cui Xinyin Han Yilin He Long Zhao Na Zhang Hao Zhang Haidong Zhu Zhexin Liu Bin Ma Shusong Zheng Wei Zhang Jiajia Liu Xiaoli Fan Yaoqi Si Shuiquan Tian Jianqing Niu Huilan Wu Xuemei Liu Zhuo Chen Deyuan Meng Xiaoyan Wang Liqiang Song Lijing Sun Jie Han Hui Zhao Jun Ji Zhiguo Wang Xiaoyu He Ruilin Li Xuebin Chi Chengzhi Liang Beifang Niu Jun Xiao Junming Li Hong-Qing Ling | 2022 | Molecular Plant2022，15，9： | 6 |
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