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| 1 | Development of mutants with varying flowering times by targeted editing of multiple SVP gene copies in Brassica napus L.显示文摘Manipulation of flowering time to develop cultivars with desired maturity dates is fundamental in plant breeding.It is desirable to generate polyploid rapeseed(Brassica napus L.)germplasm with varying flowering time controlled by a few genes.In the present study,Bna SVP,a rapeseed homolog of the Arabidopsis SVP(Short Vegetative Phase)gene,was characterized and a set of mutants was developed using a CRISPR/Cas9-based gene-editing tool.A single construct targeting multiple sites was successfully applied to precisely mutate four copies of Bna SVP.The induced mutations in these copies were stably transmitted to subsequent generations.Homozygous mutants with loss-of-function alleles and free transgenic elements were generated across the four Bna SVP homologs.All mutant T_(1)lines tested in two environments(summer and winter growing seasons)showed early-flowering phenotypes.The decrease in flowering time was correlated with the number of mutated Bna SVP alleles.The quadruple mutants showed the shortest flowering time,with a mean decrease of 40.6%–50.7%in length relative to the wild type under the two growth conditions.Our study demonstrates the quantitative involvement of Bna SVP copies in the regulation of flowering time and provides valuable resources for rapeseed breeding. | Sunny Ahmar Yungu Zhai Huibin Huang Kaidi Yu Muhammad Hafeez Ullah Khan Muhammad Shahid Rana Abdul Samad Shahid Ullah Khan Olalekan Amoo Chuchuan Fan Yongming Zhou | 2022 | The Crop Journal2022，10，1： | 1 |
| 2 | An Automated and Real-time Approach of Depression Detection from Facial Micro-expressions显示文摘Depression is a mental psychological disorder that may cause a physical disorder or lead to death.It is highly impactful on the socialeconomical life of a person;therefore,its effective and timely detection is needful.Despite speech and gait,facial expressions have valuable clues to depression.This study proposes a depression detection system based on facial expression analysis.Facial features have been used for depression detection using Support Vector Machine(SVM)and Convolutional Neural Network(CNN).We extracted micro-expressions using Facial Action Coding System(FACS)as Action Units(AUs)correlated with the sad,disgust,and contempt features for depression detection.A CNN-based model is also proposed in this study to auto classify depressed subjects from images or videos in real-time.Experiments have been performed on the dataset obtained from Bahawal Victoria Hospital,Bahawalpur,Pakistan,as per the patient health questionnaire depression scale(PHQ-8);for inferring the mental condition of a patient.The experiments revealed 99.9%validation accuracy on the proposed CNN model,while extracted features obtained 100%accuracy on SVM.Moreover,the results proved the superiority of the reported approach over state-of-the-art methods. | Ghulam Gilanie Mahmood ul Hassan Mutyyba Asghar Ali Mustafa Qamar Hafeez Ullah Rehan Ullah Khan Nida Aslam Irfan Ullah Khan | 2022 | Computers, Materials & Continua2022，，11： | 0 |
| 3 | A Robust Method of Bipolar Mental Illness Detection from Facial Micro Expressions Using Machine Learning Methods显示文摘Bipolar disorder is a serious mental condition that may be caused by any kind of stress or emotional upset experienced by the patient.It affects a large percentage of people globally,who fluctuate between depression and mania,or vice versa.A pleasant or unpleasant mood is more than a reflection of a state of mind.Normally,it is a difficult task to analyze through physical examination due to a large patient-psychiatrist ratio,so automated procedures are the best options to diagnose and verify the severity of bipolar.In this research work,facial microexpressions have been used for bipolar detection using the proposed Convolutional Neural Network(CNN)-based model.Facial Action Coding System(FACS)is used to extract micro-expressions called Action Units(AUs)connected with sad,happy,and angry emotions.Experiments have been conducted on a dataset collected from Bahawal Victoria Hospital,Bahawalpur,Pakistan,Using the Patient Health Questionnaire-15(PHQ-15)to infer a patient’s mental state.The experimental results showed a validation accuracy of 98.99%for the proposed CNN modelwhile classification through extracted featuresUsing SupportVectorMachines(SVM),K-NearestNeighbour(KNN),and Decision Tree(DT)obtained 99.9%,98.7%,and 98.9%accuracy,respectively.Overall,the outcomes demonstrated the stated method’s superiority over the current best practices. | Ghulam Gilanie Sana Cheema Akkasha Latif AnumSaher Muhammad Ahsan Hafeez Ullah Diya Oommen | 2024 | Intelligent Automation & Soft Computing2024，39，1： | 0 |
| 4 | Structural and spectroscopic analysis of wurtzite(ZnO)_(1-x)(Sb_2O_3)_x composite semiconductor显示文摘The structural, vibrational and impedance analysis for(Zn O)1 x(Sb2O3)xcomposite synthesized by solid state reaction technique were carried out in the present investigation. X-ray diffraction(XRD) study showed that(Zn O)1 x(Sb2O3)xcomposite has hexagonal(wurtzite) crystal structure. Variation in lattice constants with Sb-doping indicated the proper incorporation of Sb dopant in Zn O host matrix. The results of Raman spectroscopy test suggested the signature of E2(high) and E1(TO) Raman modes, and verified the wurtzite structure of(Zn O)1 x(Sb2O3)x composite. Two additional phonon bands(671, 712) cm 1appeared in Raman spectra of composite samples due to the existence of the lattice defects caused by Sb doping or may be other intrinsic lattice defects formed during the synthesis of(Zn O)1 x(Sb2O3)xcomposite. The frequency dependent on the electrical characteristics, such as, impedance(Z), dielectric constant(ε) and AC conductivity(σ) have been studied in a range of frequencies for different Sb concentration at room temperature. The electrical measurement results showed that the impedance increased with Sb dopant concentration, while dielectric constant and AC conductivity decreased with Sb dopant concentration. | Hafeez ullah Amjid Iqbal M. Zakria Arshad Mahmood | 2015 | Progress in Natural Science:Materials International2015，25，2： | 0 |
| 5 | 巴基斯坦北方南部地区狩猎活动对鹤类的影响(英文)显示文摘我们研究了巴基斯坦北方南部两个地区的狩猎活动对蓑羽鹤(Anthropoides virgo)和灰鹤(Grus grus)的影响。数据主要来自于野外调查、访谈及问卷调查。于2008年秋季和2009年春季分别调查了165个和85个狩猎营地。这些营地的大部分狩猎者持有野生动物狩猎许可证且狩猎活动是以休闲为目的。根据我们的调查,由于受到过度狩猎、自然栖息地的破坏、人类活动及地理因素的影响,这两种鹤在该地区的种群数量正逐渐下降。为此,我们提出了应对策略以保护这两种鹤。 | Farzana PERVEEN Hafeez Ullah KHAN | 2010 | Chinese Birds2010，，4： | 0 |
| 6 | Response of Contrasting Rice Genotypes to Zinc Sources under Saline Condition显示文摘Abiotic stresses are among the major limiting factors for plant growth and crop productivity.Among these,salinity is one of the major risk factors for plant growth and development in arid to semi-arid regions.Cultivation of salt tolerant crop genotypes is one of the imperative approaches to meet the food demand for increasing population.The current experiment was carried out to access the performance of different rice genotypes under salinity stress and Zinc(Zn)sources.Four rice genotypes were grown in a pot experiment and were exposed to salinity stress(7 dS m^(−1)),and Zn(15 mg kg^(-1)soil)was applied from two sources,ZnSO4 and Zn-EDTA.A control of both salinity and Zn was kept for comparison.Results showed that based on the biomass accumulation and K^(+)/Na^(+)ratio,KSK-133 and BAS-198 emerged as salt tolerant and salt sensitive,respectively.Similarly,based on the Zn concentration,BAS-2000 was reported as Zn-in-efficient while IR-6 was a Zn-efficient genotype.Our results also revealed that plant growth,relative water content(RWC),physiological attributes including chlorophyll contents,ionic concentrations in straw and grains of all rice genotypes were decreased under salinity stress.However,salt tolerant and Zn-in-efficient rice genotypes showed significantly higher shoot K^(+)and Zn concentrations under saline conditions.Zinc application significantly alleviates the harmful effects of salinity by improving morpho-physiological attributes and enhancing antioxidant enzyme activities,and the uptake of K and Zn.The beneficial effect of Zn was more pronounced in salt-tolerant and Zn in-efficient rice genotypes as compared with salt-sensitive and Zn-efficient genotypes.In sum,our results confirmed that Zn application increased overall plant’s performance under saline conditions,particularly in Zn in-efficient and tolerant genotypes as compared with salt-sensitive and Zn efficient rice genotypes. | Muhammad Jan Muhammad Anwar-Ul-Haq Talha Javed Sadam Hussain Ilyas Ahmad Muhammad Ashraf Sumrah Javed Iqbal Babar Hussain Babar Aqsa Hafeez Muhammad Aslam Muhammad Tahir Akbar Marjan Aziz Khadiga Alharbi Izhar Ullah | 2023 | Phyton-International Journal of Experimental Botany2023，92，5： | 0 |
| 7 | Molecular dynamics simulation of nanoscale surface diffusion of heterogeneous adatoms clusters显示文摘Molecular dynamics simulation employing the embedded atom method potential is utilized to investigate nanoscale surface diffusion mechanisms of binary heterogeneous adatoms clusters at 300 K, 500 K, and 700 K. Surface diffusion of heterogeneous adatoms clusters can be vital for the binary island growth on the surface and can be useful for the formation of alloy-based thin film surface through atomic exchange process. The results of the diffusion process show that at 300 K, the diffusion of small adatoms clusters shows hopping, sliding, and shear motion; whereas for large adatoms clusters(hexamer and above), the diffusion is negligible. At 500 K, small adatoms clusters, i.e., dimer, show almost all possible diffusion mechanisms including the atomic exchange process; however no such exchange is observed for adatoms clusters greater than dimer. At 700 K, the exchange mechanism dominates for all types of clusters, where Zr adatoms show maximum tendency and Ag adatoms show minimum or no tendency toward the exchange process. Separation and recombination of one or more adatoms are also observed at 500 K and 700 K. The Ag adatoms also occupy pop-up positions over the adatoms clusters for short intervals. At 700 K, the vacancies are also generated in the vicinity of the adatoms cluster,vacancy formation, filling, and shifting can be observed from the results. | Muhammad Imran Fayyaz Hussain Muhammad Rashid Muhammad Ismail Hafeez Ullah Yongqing Cai M Arshad Javid Ejaz Ahmad S A Ahmad | 2016 | Chinese Physics B2016，25，7： | 0 |
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