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| 1 | Preparation of an anti-diethylstilbestrol monoclonal antibody and development of an indirect competitive ELISA to detect diethylstilbestrol in biological samples显示文摘Based on the preparation of an anti-diethylstilbestrol(DES) monoclonal antibody,a simple and convenient indirect competitive enzyme-linked immunosorbent assay(ELISA) method for DES detection has been developed.The monoclonal antibody demonstrated high sensitivity to DES with an IC50 value of 275 pg mL-1 and detection limit(LOD) of 90 pg mL-1.The specificity of the assay was studied by measuring cross-reactivity of the antibody with structurally related compounds of ethinyl estradiol(<7%),estrone(<0.1%),estriol(<0.1%),and diethylstilbestrol benzoate(<0.1%).Chicken,fish,shrimp,urine and bile spiked with different concentration of DES were detected by the developed method,and the recovery rates were greater than 79.5%.Intra-and inter-assay variations were about 6%.This method exhibited high stability with a coefficient of variation less than 10% in buffer and in real samples.The LODs in fish/shrimp,liver,feed and urine spiked with DES were 600,600,4800 and 600 pg mL-1,respectively.These results confirmed that the antibody to DES was successfully produced and could be used to establish ELISA methods for DES detection in food producing animals. | LI WeiHua MENG Meng HE FangYang WAN YuPing XUE HuYin LIU Wei YIN WeiWei XU Jing FENG CaiWei WANG ShanLiang LU Xiao LIU JinTing XI RiMo | 2011 | Chinese Science Bulletin2011，56，8： | 4 |
| 2 | Cesium-rubidium mineralization in Himalayan leucogranites显示文摘This paper presents a study of a newly discovered pollucite-lepidolite-albite granite(PLAG)in the Himalayan leucogranite belt,which marks the first occurrence of pollucite,a major cesium silicate mineral,in the Himalayan orogenic belt(China).The rock appears at the northern part of the Gyirong pluton,coexisting with the tourmaline-bearing two-mica granite(TMG).Primary rare-metal minerals include lepidolite(Li),spodumene(Li),pollucite(Cs),cassiterite(Sn),and microlite(Ta).Micas,mainly lithian muscovite to lepidolite,contain 4.07 wt.%Li_2O and 0.76 wt.%Rb_2O on average.The average Li_2O content of the spodumene is 7.95 wt.%.Pollucite not only has an average Cs_2O content of 34 wt.%,but also has an elevated Rb_2O content of about 0.16 wt.%.Notably,this granite attains industrial grades for rare metals,specifically with Li_2O,Rb_2O,and Cs_2O contents of 0.49–1.19 wt.%,0.12–0.24 wt.%,and 0.69–2.33 wt.%,respectively.Dating results of magmatic accessory cassiterite and monazite indicated that the PLAG was formed at 19–18 Ma,slightly later than the TMG(22–20 Ma)of the Gyirong pluton.Thus,these two types of granites may form within the same magmatic system considering their pulsating intrusive contact,formation ages,and whole-rock and mineral chemical compositions.Furthermore,the abundant presence of pollucite suggests that the PLAG experienced high degrees of magmatic fractionation.In comparison to the Pusila spodumene pegmatite in the Himalaya and the Yashan topaz-lepidolite granite in Jiangxi,South China,the Gyirong PLAG exhibits different whole-rock and mineral compositions,resulting from differences in source materials and fractionation processes.Notably,the difference in fluorine(F)content may determine the degree of rare-metal element enrichment.The discovery of Gyirong PLAG highlights multiple stages and types of rare-metal mineralization in the Himalayan leucogranite belt,which is controlled by the South Tibetan Detachment System.The Cs-bearing geyserite deposit exposed along the Yarlung-Zangbo River,together with Himalayan leucogranites,constitutes two systems of rare-metal elements migration and enrichment.These two systems reflect the interaction among Earth systems across time and space,emphasizing how the Himalayan orogeny controls mineralization.As a result,the Himalayan leucogranite belt has considerable prospecting potential for cesium and rubidium resources and may be a crucial area for future exploration and resource utilization. | Fangyang HU Xiaochi LIU Shaoxiong HE Jiamin WANG Fuyuan WU | 2023 | Science China Earth Sciences2023，66，12： | 3 |
| 3 | Study on Colloidal Gold Immunochromatography Assay for Rapid Detection of Spectinomycin显示文摘[Objectives] This study was conducted to establish a rapid detection method for spectinomycin in pork,chicken,fish,shrimp flesh and water.[Methods]A test strip for rapid detection of spectinomycin in milk was developed by colloidal gold immunochromatography assay. [Results]The test strip had a detection limit of 50 μg/kg to milk with a detection time of 15 min,and the false positive rate and false negative rate were both 0. [Conclusions]The method is accurate,simple,reliable and convenient,and is suitable for rapid on-site spectinomycin detection. | Zhaoqin WANG Yuping WAN Xiaosheng WU Yu ZHANG Fangfang JIA Guangyao HAN Zhengxue PENG Fangyang HE | 2019 | Agricultural Biotechnology2019，8，1： | 3 |
| 4 | Generation of anti-trenbolone monoclonal antibody and establishment of an indirect competitive enzyme-linked immunosorbent assay for detection of trenbolone in animal tissues, feed and urine显示文摘 | Yuanyang Zhang Fangyang He Yuping Wan Meng Meng Jing Xu Jian Yi Yabin Wang Caiwei Feng Shanliang Wang Rimo Xi | 2010 | Talanta2010，，3： | 1 |
| 5 | Kelunteluo monoclonal antibody preparation and appraisal 显示文摘 | HE FANGYANG QIU YANGSHENG YANG GENHAI | 2001 | Chinese vet science and technology2001，31，6： | 1 |
| 6 | Generation of anti - trenbolone monoclonal antibody and establishment of an indirect competitive enzyme - linked immunosorbent assay for detection of trenbolone in animal tissues, feed and urine显示文摘 | Yuanyang Zhang Fangyang He | 2011 | Talanta2011，83，： | 1 |
| 7 | Study on Rapid Detection of Tetracyclines,Fluoroquinolones and Sulfonamides in Milk显示文摘Aiming at the market demand for rapid detection of tetracyclines,fluoroquinolones and sulfonamides in milk,a golloidal gold immunochromatography test strip for simultaneous detection of tetracyclines,fluoroquinolones and sulfonamides in milk was prepared based on the principle of competitive inhibition immunochromatography. The performance indicators of the test strip were verified. The results showed that the test strip can simultaneously detect 4 tetracyclines,13 fluoroquinolones and 13 sulfonamides,and the detection limits all can meet the national residue limits; the tests strip exhibited false positive rate≤5% and false negative rate = 0; and no cross-reaction with other drugs was commonly found in milk,indicating good specificity. The method is simple,rapid,and has low cost and easy popularization. It provides a means for realizing on-site rapid detection and is of important practical significance to guarantee of safety of milk and dairy products in China. | Yuping WAN Xiaosheng WU Tingting CUI Dongshan CAO Zhaoqin WANG Linchen WANG Chun-yan YANG Fangyang HE | 2019 | Agricultural Biotechnology2019，8，1： | 1 |
| 8 | Study on the Production of Pentachloronitrobenzene Monoclonal Antibody and Its ELISA Kit for Rapid Detection显示文摘[Objectives]This study was conducted to develop an enzyme-linked immunoassay kit that can detect the residual amount of pentachloronitrobenzene in Penaeus vannamei.[Methods]This study was conducted to develop an enzyme-linked immunoassay kit that can detect the residual amount of pentachloronitrobenzene in P.vannamei.[Results]The standard curve range of the kit was 0-8.1μg/L;the detection limit for P.vannamei was 0.912μg/kg;the recovery was 80.6%-103.5%;and the relative standard deviation range within batches was 5.3%-10.1%,and the relative standard deviation range between batches was 6.7%-8.1%.The specificity of the pentachloronitrobenzene monoclonal antibody was relatively good,and the cross-reaction rates with pentachlorophenol,hexachlorobenzene,tetrachlorophthalide,and chlorothalonil were low,all of which did not exceed 30%.The ELISA kit could be stored at 4℃for 12 months,showing good stability.[Conclusions]The detection kit has low cost,short time and small deviation,and is an ideal preliminary screening method. | Yuhua MA Kuo ZHANG Jianxiong ZHANG Fangfang JIA Fangyang HE Yanan CUI Mingyang LI Yuping WAN | 2021 | Agricultural Biotechnology2021，10，5： | 0 |
| 9 | Colloidal Gold Test Strip for the Detection of Fluoroquinolones in Foods Using an Analyzer显示文摘A test strip was developed to rapidly detect fluoroquinolones in foods by colloidal gold immunochromatography method in combination with a food safety analyzer. The results showed that the test strip has the detection sensitivity of 20 μg/L for enrofloxacin, sarafloxacin, difloxacin, ofloxacin, norfloxacin, ciprofloxacin, pefloxacin, flumequine and danofloxacin, and the detection sensitivity of 40 μg/L for enoxacin and oxolinic acid. The test strip consumes only 10 min for detection, and the false positive rate and the false negative rate are both zero. The test strip can accurately, reliably and easily detect residual fluoroquinolones in foods, and is suitable for on-site detection of a large number of samples. | Fangfang JIA Yuping WAN Linchen WANG Xiping WANG Zhaoqin WANG Fangyang HE Haifeng CUI Jing LI | 2019 | Agricultural Biotechnology2019，8，2： | 0 |
| 10 | Dual-layer vermiculite nanosheet based hybrid film to suppress dendrite growth in lithium metal batteries显示文摘Lithium metal anode has become a favorable candidate for next-generation rechargeable batteries.However, the unstable interface between lithium metal and electrolyte leads to the growth of dendrites,resulting in the low Coulombic efficiency and even the safety concerns. Herein, a rigid-flexible dual-layer vermiculite nanosheet(VN) based organic-inorganic hybrid film on lithium metal anode is proposed to suppress dendrite growth and relieve volume fluctuations. The inner mechanically robust VN layer(3 μm thick) enhances the mechanical properties of the protective layer, while the outer polymer(4 μm thick) can enhance the flexibility of the hybrid layer. The Li | Li symmetric cell with protected lithium shows an extended life of over 670 h. The full cell with Li anode protected by dual-layer interface exhibits a better capacity retention of 80% after 174 cycles in comparison to bare Li anode with 94 cycles.This study provides a novel approach and a significant step towards prolonging lifespan of lithium metal batteries. | Xiang-Qun Xu Feng-Ni Jiang Shi-Jie Yang Ye Xiao He Liu Fangyang Liu Lei Liu Xin-Bing Cheng | 2022 | Journal of Energy Chemistry2022，31，6： | 0 |
| 11 | Development of a Kind of Immunomagnetic Bead Kit to Separate and Enrich Salbutamol显示文摘An immunomagnetic bead kit to separate and enrich salbutamol was prepared through the reaction between salbutamol and 4-aminobenzoic acid.The kit had a catch of 20 ng/ml to salbutamol in samples,and showed no cross reaction with competitive drugs with structure and function similar to salbutamol: clenbuterol,ractopamine,phenylethanolamine A, bromchlorbuterol, brombuterol, terbutaline, hydroxymethyl salbutamol,cimaterol,tulobuterol,mapenterol,cimbuterol,clenpenterol,zilpaterol,penbutolol,clenproperol,mabuterol and clorprenaline. | Yuping WAN Xiaosheng WU Fangfang JIA Jing LI Zhaoqin WANG Yuejun FENG Meihong DU Fangyang HE | 2018 | Agricultural Biotechnology2018，7，6： | 0 |
| 12 | Preparation of a Salmonella LAMP Detection Kit显示文摘[Objectives] This study was conducted to establish a method for rapid detection of Salmonella at the grassroots level, and a corresponding product was developed. [Methods] By analyzing the invA gene of Salmonella, two pairs of efficient, rapid and specific amplification primers were designed and screened for the six conserved domains of the gene, and the LAMP detection kit for Salmonella based on this method was produced. [Results] The method is simple, sensitive, rapid and specific. The minimum detection concentration is 10~2-10~3 CFU/ml. [Conclusions] It can be used in the self-inspection of food production enterprises and the on-site sampling inspection and rapid detection of large quantities of samples by grassroots food safety inspection and supervision departments. | Yuping WAN Xiaosheng WU Yu ZHANG Meihong DU Zhaoqin WANG Caiwei FENG Zhengmiao ZHAO Fangyang HE | 2019 | Agricultural Biotechnology2019，8，6： | 0 |
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