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| 1 | Induction of matrix metalloproteinase-9 and-2 activity in mouse blastocyst by fibronectin-integrin interaction显示文摘Fibronectin, a major extracellular matrix, plays an important role in embryo implantation by mediating embryo adhesion and outgrowth. In this work, mouse blastocysts produced pro-matrix metalloproteinase-9, pro-matrix metalloproteinase-2 and 64 ku matrix metalloproteinase-2 when they were co-cultured with fibronectin. In contrast, mouse blastocysts did not produce these proteinases without fibronectin. Focal adhesion kinase is a fundamental molecule of integrin signaling pathway and its antisense oligodeoxynucleiotide inhibited blastocyst matrix metalloproteinases expression induced by fibronectin. The results indicated that fibronectin triggered matrix metalloproteinase-9 and -2 expression in mouse blastocyst through its integrin receptors and subsequent signaling pathway, which enhanced the synchronization of blastocyst invasiveness and uterine receptivity and ensured the accuracy of events relative to implantation in timing and spatiality. | TIE Guodong CAO Yujing ZHAO Xingxu Duan Enkui | 2000 | Chinese Science Bulletin2000，45，14： | 7 |
| 2 | Effect of fibronectin and leukaemia inhibitory factor on matrix metalloproteinases in mouse blastocyst显示文摘This study was designed to detect the effects of fibronectin (FN) and leukaemia inhibitory factor (LIF) on matrix metallopoteinases (MMPs) of mouse blastocysts. The experiments comprised four groups: first, blastocysts grew on the dishes with FN-coated; the second, without FN-coated; the third group, without FN coated, but with 20 ng/μL LIF added to culture medium; and the fourth group, with both FN-coated and 20 ng/μL LIF added. Using MMP-2 and MMP-9 primers respectively, the expressions of MMP-2 and MMP-9 were detected by RT-PCR and cloning identification. The results showed that in the first group, MMP-2 and MMP-9 were produced after 12 and 24 h culturing; in the third group, there were both MMP-2 and MMP-9 bands when blastocysts were cultured for 24 h; and in the fourth group, MMP-2 and MMP-9 bands appeared when blastocysts were cultured for 6, 12 and 24 h. But in the second group no MMP-2 or MMP-9 band appeared. These results show that FN may initiate the transcription of MMP-2 and MMP-9 genes | Jian Zhang Guodong Tie Yujing Cao Enkui Duan | 2001 | Chinese Science Bulletin2001，46，15： | 4 |
| 3 | IGF-Ⅱ and IGFBP-1 reversely regulate blastocyst implantation in mouse显示文摘Insulin-like growth factor (IGF)-Ⅱ and IGF binding protein (IGFBP)-1, members of IGF family, are important in the cyclic development of endometrium and the blastocyst implantation. In the present study, the indirect immunofluorescence showed that IGF- Ⅱ and IGFBP-1 were specifically expressed at the maternal-fetal interface. In a co-culture system, IGF-Ⅱ significantly enhanced the attachment and outgrowth of the blastocyst on monolayer of uterine epithelial cells, while IGFBP-1 did not affect the blastocyst attachment, but markedly inhibited the blastocyst outgrowth. The results of zymography showed that IGF- Ⅱ enhanced the activities of MMP-2 and MMP-9, while IGFBP-1 did not affect the activities of MMP-2 and MMP-9. In conclusion, the equilibrium between the invasion of tro-phoblast and the inhibition of deciduas may be regulated by the interaction between the IGF- Ⅱ -expressing invading cy-totrophoblast and maternal deciduas-derived IGFBP-1. | TIAN Yongqiang, TIE Guodong, CAO Yujing, ZHAO Xingxu & DUAN Enkui1. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China 2. College of Vet Medicine, Gansu Agricultural University, Lanzhou 730070, China | 2002 | Chinese Science Bulletin2002，47，21： | 3 |
| 4 | Regulation of mouse blastocyst adhesion,outgrowth and matrix metalloproteinase-2 by focal adhesion kinase显示文摘The interaction of extracellular matrix-integrin markedly influences the adhesion, outgrowth, differentiation and expression of serine proteinases by the blastocyst, so it is regarded as a vital factor in blastocyst implantation. Al- though the mechanism of extracellular interactions between extracellular matrix and integrins has been well elucidated, the roles of the signaling molecules in the extracellular ma- trix-integrin signal transduction pathway in blastocyst im- plantation are unknown. This limits the understanding of blastocyst implantation and ECM-integrin signal transduc- tion pathway. In the present study, in vitro blastocyst culture and indirect immunocytochemistry, matrix metallopro- teinases (MMPs) zymography and antisense oligodeoxynu- cleotide (ODN) were used to investigate the expression of a fundamental molecule of integrin-dependent signal trans- duction pathways, focal adhesion kinase (FAK), in mouse blastocysts and its influence on mouse blastocyst adhesion, outgrowth and MMP-2. The results showed that mouse blas- tocysts expressed FAK. FAK protein was clustered in the peripheral migrating trophoblast cells and dispersed in the central area of blastocyst outgrowth. Fibronectin triggered pro-MMP-2 and 64 kD MMP-2 activities. The antisense ODN to FAK attenuated pro-MMP-2 and 64 kD MMP-2 activities which decreased abruptly and tended to disappear with increasing concentrations of the antisense ODN. Both mouse blastocyst adhesion and outgrowth on fibronectin were also influenced by the antisense ODN. Up to 20 mg/mL of the antisense ODN concentration, the adhesion and out- growth rates were decreased in a dose-dependent manner. The results indicated that FAK influenced mouse blastocyst adhesion, outgrowth and MMP-2 activity by intracellular signal transduction. In other words, FAK regulates mouse implantation in terms of blastocyst adhesive and invasive abilities. | TIE Guodong TIAN Yongqiang CHEN Shuyi CAO Yujing LIU Zelong DUAN Enkui ZHAO Xingxu | 2003 | Chinese Science Bulletin2003，48，5： | 0 |
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