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| 1 | Efficient generation of transgenic chickens using the spermatogonial stem cells in vivo and ex vivo transfection显示文摘The highly efficient novel methods to produce transgenic chickens were established by directly in-jecting the recombinant plasmid containing green fluorescent protein (GFP) gene into the cock's testis termed as testis-medianted gene transfer (TMGT), and transplanting transfected spermatogonial stem cells (TTSSCs). For the TMGT approach,four dosages of pEGFP-N1 DNA/cationic polymer complex were injected intratesticularly. The results showed: (1) 48 h after the injection,the percentages of testis cells expressing GFP were 4.0%, 8.7%, 10.2% and 13.6% in the 50, 100, 150 and 200 μg/mL group, re-spectively. The difference from the four dosage groups was significant (P<0.05). On day 25 after the injection, a dosage-dependent and time-dependent increase in the number of transgenic sperm was observed. The percentages of gene expression reached the summit and became stable from day 70 to 160, being 12.7%, 12.8%, 15.9% and 19.1%, respectively. The difference from the four dosage groups was also significant (P<0.05). (2) 70 d after the injection, strong green fluorescent could be observed in the seminiferous tubules by whole-mount in-situ hybridization. (3) 70 d after the injection, the semen was collected and used to artificially inseminate wild-type females. The blastoderms of F1 and F2 transgenic chicken expressed GFP were 56.2% (254/452) and 53.2% (275/517), respectively. The detec-tion of polymerase chain reaction (PCR) of F1 and F2 transgenic chicken blood genomic DNA showed that 56.5% (3/23) of F1 and 52.9% (9/17) of F2 were positive. Southern blot showed GFP DNA was in-serted in their genomic DNAs. (4) Frozen whole mount tissue sections of F1 and F2 transgenic chicken liver, heart, kidney and muscle showed that the rates of green fluorescent positive were between 50.0% and 66.7%. (5) With the TTSSCs method, SSCs ex vivo transfected with GFP were transplanted into recipient roosters whose endogenic SSCs had been resoluted. The donor SSCs settled and GFP ex-pression became readily detectable in the frozen whole mount tissue sections of recepient testes. Moreover, sperms carrying GFP could be produced normally. The results of artificially inseminating wild-type females with these sperms showed 12.5% (8/64) of offspring embryo expressed GFP and 11.1% (2/18) hatched chicks were tested transgenic. Our data therefore suggest TMGT and TTSSCs are the feasible methods for the generation of transgenic chickens. | LI BiChun SUN GuoBo SUN HuaiChang XU Qi GAO Bo ZHOU GuanYue ZHAO WenMing WU XinSheng BAO WenBin YU Fei WANG KeHua CHEN GuoHong | 2008 | Science China(Life Sciences)2008，51，8： | 13 |
| 2 | Cytotoxic Effect on Osteosarcoma MG-63 Cells by Degradation of Magnesium显示文摘Biocompatible and biodegradable magnesium(Mg)based metals have attracted great interest for use in orthopedic implants and devices.Based on our previous study that Mg with and without micro-arc oxidation(MAO)coating showed obvious cytotoxic effect on tumor cells due to the increase of pH value during the degradation of Mg,this study further evaluated the cytotoxic effect of Mg and MAO coated Mg on osteosarcoma(MG-63)cells by analyzing the cell adhesion,morphology and number through observation of scanning electron microscope,as well as live/dead staining,lactate dehydrogenase(LDH)activity and 4,6-diamidino-2-phenylindole(DAPI)assay.The results indicated that,compared to titanium,Mg could strongly inhibit the cell adherence,morphology and number of MG-63 on the surface of the naked Mg,whereas the MAO coated Mg showed relative weak cytotoxic effect on MG-63 cells,expecting that magnesium based metals with suitable coating can be designed to be applied as tumor prosthesis in the clinical practice. | Mei Li Ling Ren LiHua Li Peng He GuoBo Lan Yu Zhang Ke Yang | 2014 | Journal of Materials Science & Technology2014，30，9： | 7 |
| 3 | Sirtuin 5:a review of structure,known inhibitors and clues for developing new inhibitors显示文摘Sirtuins(SIRTs) are nicotinamide adenine dinucleotide(NAD^+)-dependent protein deacetylases,which regulate important biological processes ranging from apoptosis,age-associated pathophysiologies,adipocyte and muscle differentiation,and energy expenditure to gluconeogenesis.Very recently,sirtuin 5(SIRT5) has received considerable attention due to that it was found to have weak deacetylase activity but strong desuccinylase,demalonylase and deglutarylase activities,and it was also found to be associated with several human diseases such as cancer,Alzheimer's disease,and Parkinson's disease.In this review,we for the first time summarized the structure characteristics,known peptide and small-molecule inhibitors of SIRT5,extracted some clues from current available information and introduced some feasible,practical in silico methods,which might be useful in further efforts to develop new SIRT5 inhibitors. | Lingling Yang Xiaobo Ma Yanying He Chen Yuan Quanlong Chen Guobo Li Xianggui Chen | 2017 | Science China(Life Sciences)2017，60，3： | 7 |
| 4 | Promoting Bone Mesenchymal Stem Cells and Inhibiting Bacterial Adhesion of Acid-Etched Nanostructured Titanium by Ultraviolet Functionalization显示文摘Titanium(Ti) and its alloys are used extensively in orthopedic implants because of their excellent biocompatibility,mechanical properties and corrosion resistance. However,titanium-based implant materials face many severe complications,such as implant loosening due to poor osseointegration and bacterial infections,which may lead to implant failure. Hence,preparing a biomaterial surface,which enhances the interactions with host cells and inhibits bacterial adhesion,may be an optimal strategy to reduce the incidence of implant failure. This study aims to improve osseointegration and confer antibacterial properties on Ti through a combination of two surface modifications including nanostructuring generated by acid etching and ultraviolet(UV) light treatment.Our results showed that without UV treatment,the acid etching treatment of Ti surface was effective at both improving the adhesion of bone mesenchymal stem cells(BMSCs) and increasing bacterial adhesion. A further UV treatment of the acid-etched surface however,not only significantly improved the cell adhesion but also inhibited bacterial adhesion. The acid-etched nanostructured titanium with UV treatment also showed a significant enhancement on cell proliferation,alkaline phosphatase(ALP) activity and mineralization. These results suggest that such nanostructured materials with UV treatment can be expected to have a good potential in orthopedic applications. | Guobo Lan Mei Li Ying Tan Lihua Li Xiaoming Yang Limin Ma Qingshui Yin Hong Xia Yu Zhang Guoxin Tan Chengyun Ning | 2015 | Journal of Materials Science & Technology2015，31，2： | 7 |
| 5 | Quantitativegeneticanalysisstationforthegeneticanalysisofcomplextraits显示文摘The Quantitative Genetic Analysis Station (QGAStation) is a software package that has been developed to perform statistical analysis for complex traits.It consists of five domains for handling data from diallel crosses,regional trials,core germplasm collections,QTL mapping,and microarray experiments.The first domain contains genetic models for diallel cross analysis,in which genetic variance components and genetic-by-environment interactions can be estimated,and genetic effects can be predicted.The second domain evaluates the performance of varieties in regional trials by implementing a general statistical method that outperforms ANOVA in tackling unbalanced data that arises frequently in trials across multiple locations and over a number of years.The third domain,using predicted genotypic values as proxy,constructs core germplasm collections covering sufficient genetic diversity with lower redundancy.The fourth domain manages genotypic and phenotypic data for QTL mapping.Linkage maps can be constructed and genetic distances can be estimated;the statistical methods that have been implemented apply to both chiasmatic and achiasmatic organisms.Another part of this domain can filter systematic noises in phenotypic data.The fifth domain focuses on the cDNA expression data that is generated by microarray experiments.A two-step strategy has been implemented to detect differentially expressed genes and to estimate their effects.Except in the fourth domain,the major statistical methods that have been used are mixed linear model approaches that have been implemented in the C language.Computational efficiency is further boosted for computers that are equipped with graphics processing units (GPUs).A user friendly graphic interface is provided for Microsoft Windows and Apple Mac operating systems.QGAStation is available at http://gffzzbe43f054107d49d7hc555xbqccqu96uuk.ffgz.tsg.suse.edu.cn/software/qga/. | CHEN GuoBo ZHU ZhiXiang ZHANG FuTao ZHU Jun | 2012 | Chinese Science Bulletin2012，57，21： | 6 |
| 6 | NAD+metabolism:pathophysiologic mechanisms and therapeutic potential显示文摘Nicotinamide adenine dinucleotide(NAD^(+))and its metabolites function as critical regulators to maintain physiologic processes,enabling the plastic cells to adapt to environmental changes including nutrient perturbation,genotoxic factors,circadian disorder,infection,inflammation and xenobiotics.These effects are mainly achieved by the driving effect of NAD^(+)on metabolic pathways as enzyme cofactors transferring hydrogen in oxidation-reduction reactions.Besides,multiple NAD^(+)-dependent enzymes are involved in physiology either by post-synthesis chemical modification of DNA,RNA and proteins,or releasing second messenger cyclic ADPribose(cADPR)and NAADP^(+).Prolonged disequilibrium of NAD^(+)metabolism disturbs the physiological functions,resulting in diseases including metabolic diseases,cancer,aging and neurodegeneration disorder.In this review,we summarize recent advances in our understanding of the molecular mechanisms of NAD^(+)-regulated physiological responses to stresses,the contribution of NAD^(+)deficiency to various diseases via manipulating cellular communication networks and the potential new avenues for therapeutic intervention. | Na Xie Lu Zhang Wei Gao Canhua Huang Peter Ernst Huber Xiaobo Zhou Changlong Li Guobo Shen Bingwen Zou | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 5 |
| 7 | Global satisfactory control for nonlinear integrator processes with long delay显示文摘有长延期的综合者过程是困难的控制。致动器的 Nonlinearcharacteristics 使控制问题更挑战性。一种技术与继电器类型非线性为如此的进程的全球令人满意的控制(GSC ) 在这篇论文被建议。Anoscillatory 控制信号被注入非线性的进程;摆动的信号的振幅和频率被设计排队产生在泛音分析的意义的非线性的过程;并且一个州的反馈控制器被设置在 linearisedprocess 上实现 GSC。一个解说性的例子被给表明建议方法的有效性。 | Yiqun YANG Guobo XIANG | 2007 | 控制理论与应用(英文版)2007，5，2： | 4 |
| 8 | Structural basis of the Norrin-Frizzled 4 interaction显示文摘 | Guobo Shen Jiyuan Ke Zhizhi Wang Zhihong Cheng Xin Gu Yuquan Wei Karsten Melcher H Eric Xu Wenqing Xu | 2015 | Cell Research2015，25，9： | 3 |
| 9 | Closed-form solution of beam on Pasternak foundation under inclined dynamic load显示文摘The dynamic response of an infinite Euler–Bernoulli beam resting on Pasternak foundation under inclined harmonic line loads is developed in this study in a closed-form solution.The conventional Pasternak foundation is modeled by two parameters wherein the second parameter can account for the actual shearing effect of soils in the vertical direction.Thus,it is more realistic than the Winkler model,which only represents compressive soil resistance.However,the Pasternak model does not consider the tangential interaction between the bottom of the beam and the foundation;hence,the beam under inclined loads cannot be considered in the model.In this study,a series of horizontal springs is diverted to the face between the bottom of the beam and the foundation to address the limitation of the Pasternak model,which tends to disregard the tangential interaction between the beam and the foundation.The horizontal spring reaction is assumed to be proportional to the relative tangential displacement.The governing equation can be deduced by theory of elasticity and Newton’s laws,combined with the linearly elastic constitutive relation and the geometric equation of the beam body under small deformation condition.Double Fourier transformation is used to simplify the geometric equation into an algebraic equation,thereby conveniently obtaining the analytical solution in the frequency domain for the dynamic response of the beam.Double Fourier inverse transform and residue theorem are also adopted to derive the closed-form solution.The proposed solution is verified by comparing the degraded solution with the known results and comparing the analytical results with numerical results using ANSYS.Numerical computations of distinct cases are provided to investigate the effects of the angle of incidence and shear stiffness on the dynamic response of the beam.Results are realistic and can be used as reference for future engineering designs. | Yu Miao Yang Shi Guobo Wang Yi Zhong | 2017 | Acta Mechanica Solida Sinica2017，30，6： | 2 |
| 10 | Histones released by NETosis enhance the infectivity of SARS-CoV-2 by bridging the spike protein subunit 2 and sialic acid on host cells显示文摘Neutrophil extracellular traps(NETs)can capture and kill viruses,such as influenza viruses,human immunodeficiency virus(HIV),and respiratory syncytial virus(RSV),thus contributing to host defense.Contrary to our expectation,we show here that the histones released by NETosis enhance the infectivity of SARS-CoV-2,as found by using live SARS-CoV-2 and two pseudovirus systems as well as a mouse model.The histone H3 or H4 selectively binds to subunit 2 of the spike(S)protein,as shown by a biochemical binding assay,surface plasmon resonance and binding energy calculation as well as the construction of a mutant S protein by replacing four acidic amino acids.Sialic acid on the host cell surface is the key molecule to which histones bridge subunit 2 of the S protein.Moreover,histones enhance cell-cell fusion.Finally,treatment with an inhibitor of NETosis,histone H3 or H4,or sialic acid notably affected the levels of sgRNA copies and the number of apoptotic cells in a mouse model.These findings suggest that SARS-CoV-2 could hijack histones from neutrophil NETosis to promote its host cell attachment and entry process and may be important in exploring pathogenesis and possible strategies to develop new effective therapies for COVID-19. | Weiqi Hong Jingyun Yang Jun Zou Zhenfei Bi Cai He Hong Lei Xuemei He Xue Li Aqu Alu Wenyan Ren Zeng Wang Xiaohua Jiang Kunhong Zhong Guowen Jia Yun Yang Wenhai Yu Qing Huang Mengli Yang Yanan Zhou Yuan Zhao Dexuan Kuang Junbin Wang Haixuan Wang Siyuan Chen Min Luo Ziqi Zhang Tiangi Lu Li Chen Haiying Que Zhiyao He Qiu Sun Wei Wang Guobo Shen Guangwen Lu Zhiwei Zhao Li Yang Jinliang Yang Zhenling Wang Jiong Li Xiangrong Song Lunzhi Dai Chong Chen Jia Geng Maling Gou Lu Chen Haohao Dong Yong Peng Canhua Huang Zhiyong Qian Wei Cheng Changfa Fan Yuquan Wei Zhaoming Su Aiping Tong Shuaiyao Lu Xiaozhong Peng Xiawei Wei | 2022 | Cellular & Molecular Immunology2022，19，5： | 2 |
| 11 | Numerical study on the seismic response of the underground subway station-surrounding soil mass-ground adjacent building system显示文摘 | Guobo WANG Mingzhi YUAN Xianfeng MA Jun WU | 2017 | Frontiers of Earth Science2017，11，4： | 2 |
| 12 | Neoantigens:promising targets for cancer therapy显示文摘Recent advances in neoantigen research have accelerated the development and regulatory approval of tumor immunotherapies,including cancer vaccines,adoptive cell therapy and antibody-based therapies,especially for solid tumors.Neoantigens are newly formed antigens generated by tumor cells as a result of various tumor-specific alterations,such as genomic mutation,dysregulated RNA splicing,disordered post-translational modification,and integrated viral open reading frames.Neoantigens are recognized as non-self and trigger an immune response that is not subject to central and peripheral tolerance.The quick identification and prediction of tumor-specific neoantigens have been made possible by the advanced development of next-generation sequencing and bioinformatic technologies.Compared to tumor-associated antigens,the highly immunogenic and tumor-specific neoantigens provide emerging targets for personalized cancer immunotherapies,and serve as prospective predictors for tumor survival prognosis and immune checkpoint blockade responses.The development of cancer therapies will be aided by understanding the mechanism underlying neoantigen-induced anti-tumor immune response and by streamlining the process of neoantigen-based immunotherapies.This review provides an overview on the identification and characterization of neoantigens and outlines the clinical applications of prospective immunotherapeutic strategies based on neoantigens.We also explore their current status,inherent challenges,and clinical translation potential. | Na Xie Guobo Shen Wei Gao Zhao Huang Canhua Huang Li Fu | 2023 | Signal Transduction and Targeted Therapy2023，8，2： | 2 |
| 13 | Low levels of neutralizing antibodies against XBB Omicron subvariants after BA.5 infection显示文摘The COVID-19 response strategies in Chinese mainland were recently adjusted due to the reduced pathogenicity and enhanced infectivity of Omicron subvariants.In Chengdu,China,an infection wave was predominantly induced by the BA.5 subvariant.It is crucial to determine whether the hybrid anti-SARS-CoV-2 immunity following BA.5 infection. | Jingyun Yang Weiqi Hong Hong Lei Cai He Wenwen Lei Yanan Zhou Tingmei Zhao Aqu Alu Xuelei Ma Jiong Li Li Yang Zhenling Wang Wei Wang Guangwen Lu Guobo Shen Shuaiyao Lu Guizhen Wu Huashan Shi Xiawei Wei | 2023 | Signal Transduction and Targeted Therapy2023，8，7： | 1 |
| 14 | Modeling China' Demand f or International Reserves显示文摘 | Guobo Huang | 1995 | Applied Financial Economies1995，，5： | 1 |
| 15 | Open-loop Matrix A,Optimum Construction and Influence of Nonlinearity on Stability of Optimum System显示文摘 | Guobo Xiang | 1982 | Scientia Sinica(Series A)1982，25，5： | 1 |
| 16 | How can graphene reduce the flammability of polymer nanocomposites显示文摘 | Huang Guobo Gao Jianrong Wang Xu | 2012 | Materials Letters2012，66，1： | 1 |
| 17 | A novel intumescent flame retardant-functionalized graphene:Nano- composite synthesis, characterization, and flammability properties显示文摘 | Huang Guobo Chen Suqing Tang Shouwan | 2012 | Materials Chemistry and Physics2012，135，23： | 1 |
| 18 | Adsorption and enrichment of alkalids from areca nut by means of macroporous resins显示文摘 | DING Yan YANG Xiaoming PAN Guobo | 2012 | Adsorption Science & Technology2012，30，3： | 1 |
| 19 | Combination effect of organics-modified montmorillonite with intumescent flame retardants on thermal stability and fire behavior of po- lyethylene nanocomposites 显示文摘 | HUANG Guobo ZHU Bingchun SHI Haibo | 2011 | J Appl Polym Sci2011，131，3： | 1 |
| 20 | New techniques and strategies in drug discovery显示文摘Great success has been witnessed in last decades,some new techniques and strategies have been widely used in drug discovery.In this roadmap,several representative techniques and strategies are highlighted to show recent advances in this filed.(A)A DOX protocol has been developed for accurate protein-ligand binding structure prediction,in which first principle method was used to rank the binding poses.Validation against crystal structures have found that DOX prediction achieved an impressive success rate of 99%,indicating significant improvement over molecular docking method.(B)Virtual target profiling is a compound-centric strategy enabling a parallel implementation of interrogating compounds against various targets in a single screen,which has been used in hit/lead identification,drug repositioning,and mechanism-of-action studies.Current and emerging methods for virtual target profiling are briefly summarized herein.(C)Research on targeted autophagy to treat diseases has received encouraging progress.However,due to the complexity of autophagy and disease,experimental and in silico methods should be performed synergistically for the entire process.This part focuses on in silico methods in autophagy research to promote their use in medicinal research.(D)Histone deacetylases(HDACs)play important roles in various biological functions through the deacetylation of lysine residues.Recent studies demonstrated that HDACs,which possess low deacetylase activities,exhibited more efficient defatty-acylase activities.Here,we review the defatty-acylase activity of HDACs and describe examples for the design of isoform selective HDAC inhibitor.(E)The FDA approval of three kinase allosteric inhibitors and some others entering clinical study has spurred considerable interests in this targeted drug discovery area.(F)Recent advances are reviewed in structure-based design of novel antiviral agents to combat drug resistance.(G)Since nitric oxide(NO)exerts anticancer activity depending on its concentration,optimal levels of NO in cancer cells is desirable.In this minireview,we briefly describe recent advances in the research of NO-based anticancer agents by our group and present some opinions on the future development of these agents.(H)The field of photoactivation strategies have been extensively developed for controlling chemical and biological processes with light.This review will summarize and provide insight into recent research advances in the understanding of photoactivatable molecules including photoactivatable caged prodrugs and photoswitchable molecules. | Jintong Du Jing Guo Dongwei Kang Zhihong Li Guan Wang Jianbing Wu Zhen Zhang Hao Fang Xuben Hou Zhangjian Huang Guobo Li Xiaoyun Lu Xinyong Liu Liang Ouyang Li Rao Peng Zhan Xiaojin Zhang Yihua Zhang | 2020 | Chinese Chemical Letters2020，31，7： | 1 |
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