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| 1 | Karyotype Stability and Unbiased Fractionation in the Paleo-Allotetraploid Cucurbita Genomes显示文摘 | Honghe Sun Shan WU Guoyu Zhang Chen Jiao Shaogui Guo Yi Ren Jie Zhang Haiying Zhang Guoyi Gong Zhangcai Jia Fan Zhang Jiaxing Tian William J. Lucas Jeff J. Doyle Haizhen Li Zhangjun Fei Yong Xu | 2017 | Molecular Plant2017，10，10： | 33 |
| 2 | Overexpression of the Watermelon Ethylene Response Factor ClERF069 in Transgenic Tomato Resulted in Delayed Fruit Ripening显示文摘Watermelon fruit undergoes distinct development stages with dramatic changes during fruit ripening.To date,the molecular mechanics of watermelon ripening remain unclear.Genetic and transcriptome evidences suggested that the ethylene response factor(ERF)gene ClERF069 may be an important candidate factor affecting watermelon fruit ripening.To dissect the roles of ClERF069 in fruit ripening,structure and phylogenetic analysis were performed using the amplified full-length sequence.Normal-ripening watermelon 97103,non-ripening watermelon PI296341-FR and the RIL population were used to analyze ClERF069 expression dynamics and the correlation with fruit ripening indexs.The results indicated that ClERF069 belongs to ERF family group VI and show high homology(83%identity)to melon ERF069-like protein.ClERF069 expression in watermelon flesh was negatively correlated with fruit lycopene content and sugar content during fruit ripening progress.Further transgenic evidences indicated that overexpression of 35S:ClERF069 in tomato noticeably delayed the ripening process up to 5.2 days.Lycopene,β-carotenoid accumulation patterns were altered and ethylene production patterns in transgenic fruits was significantly delayed during fruit ripening.Taken together,watermelon ethylene response factor ClERF069 was concluded to be a negative regulator of fruit ripening. | Ming Zhou Shaogui Guo Shouwei Tian Jie Zhang Yi Ren Guoyi Gong Changbao Li Haiying Zhang Yong Xu | 2020 | Horticultural Plant Journal2020，6，4： | 9 |
| 3 | Modulation of Sex Expression in Four Forms of Watermelon by Gibberellin, Ethephone and Silver Nitrate显示文摘There has been no systematic research on the effect of plant growth regulators and silver nitrate treatments on the control of sex expression in watermelon. In this study, we tested sex expression responses of four watermelon forms(monoecism, gynoecism, andromonoecism,and hermaphrodite) to gibberellin, ethephon and silver nitrate treatments. Results have shown that, in monoecious plants, gibberellins(GA_3)and ethephon treatments reduced the percentage of female flowers and delayed the occurrence of the first female flower, while silver nitrate induced the formation of bisexual flowers. In gynoecious plants, both ethephon and silver nitrate treatments transformed some female flowers into bisexual flowers, and treatment with ethephon resulted in a mass of abnormal flowers, while no obvious effect of treatment with GA_3 was observed. In andromonoecious plants, ethephon and GA_3 treatments delayed the occurrence of the first bisexual flower, and GA_3 reduced the percentage of bisexual flowers, while no distinct effect for silver nitrate treatment was observed. In hermaphroditic plants, ethephon treatment induced the appearance of numerous abnormal flowers, while no obvious effects for GA_3 and silver nitrate treatments were observed.We analyzed the transcription levels of all the expressed aminocyclopropane-1-carboxylic acid synthase(ACS) homologues in two gynoecious mutants and their wild types. We also tested the gene expression of CitACS4 which had been recognized as the andromonoecious gene in all treatments. All these results suggested that the best masculinizing treatment for breeding of the gynoecious line is silver nitrate, which repressed the expression of CitACS4 and induced many bisexual flowers for use in self-fertilization subsequently. | ZHANG Jie SHI Jianting JI Gaojie ZHANG Haiying GONG Guoyi GUO Shaogui REN Yi FAN Jianguang TIAN Shouwei XU Yong | 2017 | Horticultural Plant Journal2017，3，3： | 6 |
| 4 | Correction: The NAC transcription factor ClNAC68 positively regulates sugar content and seed development in watermelon by repressing ClINV and ClGH3.6显示文摘In this article 1,author name Yanping Wang was incorrectly tagged as corresponding author.Prof.Wang should be the co-first author,instead of the corresponding author.The original article has been corrected. | Jinfang Wang Yanping Wang Jie Zhang Yi Ren Maoying Li Shaowei Tian Yongtao Yu Yi Zuo Guoyi Gong Haiying Zhang Shaogui Guo Yong Xu | 2021 | Horticulture Research2021，8，1： | 2 |
| 5 | The ieasibility of enhanced soil washing ofp-nitrochlorobenzene(pNCB)with SDBS/Tween80 mixed surfactants显示文摘 | GUO Huiqin LIU Zhenyu YANG Shaogui | 2009 | Journal of Hazardous Materials2009，170，23： | 1 |
| 6 | Identification and validation of a core set of microsatellite markers for genetic diversity analysis in watermelon, Citrullus lanatus Thunb. Matsum. & Nakai显示文摘 | Haiying Zhang Hui Wang Shaogui Guo Yi Ren Guoyi Gong Yiqun Weng Yong Xu | 2012 | Euphytica2012，，2： | 1 |
| 7 | Transcriptome sequenc- ing and comparative analysis of cucumber flowers with different sex types显示文摘 | Guo Shaogui Zheng Yi Joung J G | 2010 | BMC Genomics2010，11，： | 1 |
| 8 | Characterization of tran- scriptome dynamics during watermelon fruit development : sequenc- ing, assembly,annotation and geneexpression profiles显示文摘 | Guo Shaogui Liu Jingan Zheng Yi | 2011 | BMC Ge- nomics2011，12，： | 1 |
| 9 | CRISPR/Cas9-mediated mutagenesis of CiBG1 decreased seed size and promoted seed germination in watermelon显示文摘Abscisic acid(ABA)is a critical regulator of seed development and germination.β-glucosidases(BGs)have been suggested to be contributors to increased ABA content because they catalyze the hydrolysis of ABA-glucose ester to release free ABA.However,whether BGs are involved in seed development is unclear.In this study,a candidate gene,CiBG1,in watermelon was selected for targeted mutagenesis via the CRISPR/Cas9 system.Seed size and weight were significantly reduced in the Clbg1-mutant watermelon lines,which was mainly attributed to decreased cell number resulting from decreased ABA levels.A transcriptome analysis showed that the expression of 1015 and 1429 unique genes was changed 10 and 18 days after pollination(DAP),respectively.Cytoskeleton-and cell cycle-related genes were enriched in the differentially expressed genes of wild type and Clbg1-mutant lines during seed development.Moreover,the expression of genes in the major signaling pathways of seed size control was also changed.In addition,seed germination was promoted in the Cibg1-mutant lines due to decreased ABA content.These results indicate that ClBG1 may be critical for watermelon seed size regulation and germination mainly through the modulation of ABA content and thereby the transcriptional regulation of cytoskeleton-,cell cycle-and signaling-related genes.Our results lay a foundation for dissecting the molecular mechanisms of controlling watermelon seed size,a key agricultural trait of significant economic importance. | Yanping Wang Jinfang Wang Shaogui Guo Shouwei Tian Jie Zhang Yi Ren Maoying Li Guoyi Gong Haiying Zhang Yong Xu | 2021 | Horticulture Research2021，8，1： | 1 |
| 10 | Natural variation in the NAC transcription factor NONRIPENING contributes to melon fruit ripening显示文摘ThThe NAC transcription factor NONRIPENING(NOR)is a master regulator of climacteric fruit ripening.Melon(Cucumis melo L.)has climacteric and nonclimacteric fruit ripening varieties and is an ideal model to study fruit ripening.Two natural CmNAC-NOR variants,the climacteric haplotype CmNAC-NOR^(S,N) and the non-climacteric haplotype CmNAC-NOR^(A,S),have effects on fruit ripening;however,their regulatory mechanisms have not been elucidated.Here,we report that a natural mutation in the transcriptional activation domain of CmNAC-NORS,Ncontributes to climacteric melon fruit ripening.CmNAC-NOR knockout in the climacteric-type melon cultivar“BYJH”completely inhibited fruit ripening,while ripening was delayed by 5-8 d in heterozygous cmnac-nor mutant fruits.CmN AC-NOR directly activated carotenoid,ethylene,and abscisic acid biosynthetic genes to promote fruit coloration and ripening.Furthermore,CmNAC-NOR mediated the transcription of the“CmNAC-NOR-CmNAC73-CmCWINV2”module to enhance flesh sweetness.The transcriptional activation activity of the climacteric haplotype CmNAC-NORS,Non these target genes was significantly higher than that of the nonclimacteric haplotype CmNAC-NOR^(A,S).Moreover,CmNAC-NORS,Ncomplementation fully rescued the non-ripening phenotype of the tomato(Solanum lycopersicum)cr-nor mutant,while CmNAC-NOR^(A,S) did not.Our results provide insight into the molecular mechanism of climacteric and non-climacteric fruit ripening in melon. | Jinfang Wang Shouwei Tian Yongtao Yu Yi Ren Shaogui Guo Jie Zhang Maoying Li Haiying Zhang Guoyi Gong Min Wang Yong Xu | 2022 | Journal of Integrative Plant Biology2022，64，7： | 1 |
| 11 | Identification and validation of a core set of microsatellite markers for genetic diversity analysis in watermelon, Citrullus lanatus Thunb. Matsum. & Nakai显示文摘 | Haiying Zhang Hui Wang Shaogui Guo Yi Ren Guoyi Gong Yiqun Weng Yong Xu | 2012 | Euphytica2012，，2： | 1 |
| 12 | Photolytic destruction of endocrine disruptor atrazine in aqueous solution under UV irradiation:products and pathways显示文摘 | Chen Cheng Yang Shaogui Guo Yaping | 2009 | Journal of Hazardous Materials2009，172，： | 1 |
| 13 | Mutation in the gene encoding 1-aminocyclopropane-1-carboxylate synthase 4 (CitACS4) led to andromonoecy in watermelon显示文摘Although it has been reported previously that ethylene plays a critical role in sex determination in cucurbit species, how the andromonoecy that carries both the male and hermaphroditic flowers is determined in watermelon is still unknown. Here we showed that the watermelon gene 1-aminocyclopropane-1-carboxylate synthase 4(Cit ACS4), expressed specifically in carpel primordia, determines the andromonoecy in watermelon. Among four single nucleotide polymorphism(SNPs) and one InDel identified in the coding region of Cit ACS4, the C364 W mutation located in the conserved box 6 was cosegregated with andromonoecy. Enzymatic analyses showed that the C364 W mutation caused a reduced activity in Cit ACS4. We believe that the reduced Cit ACS4 activity may hamper the programmed cell death in stamen primordia, leading to the formation of hermaphroditic flowers. | Gaojie Ji Jie Zhang Haiying Zhang Honghe Sun Guoyi Gong Jianting Shi Shouwei Tian Shaogui Guo Yi Ren Huolin Shen Junping Gao Yong Xu | 2016 | Journal of Integrative Plant Biology2016，58，9： | 1 |
| 14 | Dissipative particle dynamics simulation study of the bilayer-vesicle transition显示文摘A bilayer structure is an important immediate for the vesicle formation. However,the mechanism for the bilayer-vesicle transition remains unclear. In this work,a dissipative particle dynamics(DPD) simulation method was employed to study the mechanism of the bilayer-vesicle transition. A coarse-grained model was built based on a lipid molecule termed dimyristoylphosphatidylcholine(DMPC). Simulations were performed from two different initial configurations:a random dispersed solution and a tensionless bilayer. It was found that the bilayer-vesicle transition was driven by the minimization of the water-tail hydrophobic interaction energy,and was accompanied with the increase of the position entropy due to the redistribution of water molecules. The bulk pressure was reduced during the bilayer-vesicle transition,suggesting the evolved vesicle morphology was at the relatively low free energy state. The membrane in the product vesicle was a two-dimensional fluid. It can be concluded that the membrane of a vesicle is not interdigitated and most of the bonds in lipid chains are inclined to orient along the radical axis of the vesicle. | WU ShaoGui GUO HongXia | 2008 | Science China Chemistry2008，51，8： | 1 |
| 15 | ClSnRK2.3 negatively regulates watermelon fruit ripening and sugar accumulation显示文摘Watermelon(Citrullus lanatus) as non-climacteric fruit is domesticated from the ancestors with inedible fruits. We previously revealed that the abscisic acid(ABA) signaling pathway gene ClSnRK2.3 might infuence watermelon fruit ripening. However,the molecular mechanisms are unclear. Here,we found that the selective variation of ClSnRK2.3 resulted in lower promoter activity and gene expression level in cultivated watermelons than ancestors, which indicated ClSnRK2.3 might be a negative regulator in fruit ripening. Overexpression (OE) of ClSnRK2.3 significantly delayed watermelon fruit ripening and suppressed the accumulation of sucrose, ABA and gibberellin GA4. Furthermore,we determined that the pyrophosphate-dependent phosphofructokinase(ClPFP1) in sugar metabolism pathway and GA biosynthesis enzyme GA20 oxidase(Cl GA20ox) could be phosphorylated by ClSnRK2.3 and thereby resulting in accelerated protein degradation in OE lines and finally led to low levels of sucrose and GA4. Besides that, ClSnRK2.3 phosphorylated homeodomain-leucine zipper protein(ClHAT1) and protected it from degradation to suppress the expression of the ABA biosynthesis gene 9’-cis-epoxycarotenoid dioxygenase 3(Cl NCED3). These results indicated that ClSnRK2.3 negatively regulated watermelon fruit ripening by manipulating the biosynthesis of sucrose, ABA and GA4. Altogether, these findings revealed a novel regulatory mechanism in non-climacteric fruit development and ripening. | Jinfang Wang Yanping Wang Yongtao Yu Jie Zhang Yi Ren Shouwei Tian Maoying Li Shengjin Liao Shaogui Guo Guoyi Gong Haiying Zhang Yong Xu | 2023 | Journal of Integrative Plant Biology2023，65，10： | 0 |
| 16 | The NAC transcription factor CINAC68 positively regulates sugar content and seed development in watermelon by repressing CIINV and CIGH3.6显示文摘NAC(NAM,ATAF1/2,and CUC2)transcription factors play important roles in fruit ripening and quality.The watermelon genome encodes 80 NAC genes,and 21 of these NAC genes are highly expressed in both the flesh and vascular tissues.Among these genes,CINAC68 expression was signi fi cantly higher in flesh than in rind.However,the intrinsic regulatory mechanism of CINAC68 in fruit ripening and quality is still unknown.In this study,we found that ClNAC68 is a transcriptional repressor and that the repression domain is located in the C-terminus.Knockout of CINAC68 by the CRISPR-Cas9 system decreased the soluble solid content and sucrose accumulation in mutant flesh.Development was delayed,germination was inhibited,and the IAA content was signi ficantly decreased in mutant seeds.Transcriptome analysis showed that the invertase gene CUNV was the only gene involved in sucrose metabolism that was upregulated in mutant flesh,and expression of the indole-3-acetic acid-amido synthetase gene CIGH3.6 in the IAA signaling pathway was also induced in mutant seeds.EMSA and dual-luciferase assays showed that CINAC68 directly bound to the promoters of CUNV and CIGH3.6 to repress their expression.These results indicated that CINAC68 positively regulated sugar and IAA accumulation by repressing CUNV and CIGH3.6.Our findings provide new insights into the regulatory mechanisms by which NAC transcription factors affect fruit quality and seed development. | Jinfang Wang Yanping Wang Jie Zhang Yi Ren Maoying Li Shaowei Tian Yongtao Yu Yi Zuo Guoyi Gong Haiying Zhang Shaogui Guo Yong Xu | 2021 | Horticulture Research2021，8，1： | 0 |
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