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| 1 | Neural EGF-like protein 1(NELL-1):Signaling crosstalk in mesenchymal stem cells and applications in regenerative medicine显示文摘Bone tissue regeneration holds the potential to solve both osteoporosis and large skeletal defects,two problems associated with significant morbidity.The differentiation of mesenchymal stem cells into the osteogenic lineage requires a specific microenvironment and certain osteogenic growth factors.Neural EGF Like-Like molecule 1(NELL-1)is a secreted glycoprotein that has proven,both in vitro and in vivo,to be a potent osteo-inductive factor.Furthermore,it has been shown to repress adipogenic differentiation and inflammation.NELL-1 can work synergistically with other osteogenic factors such as Bone Morphogenic Protein(BMP)2 and9,and has shown promise for use in tissue engineering and as a systemically administered drug for the treatment of osteoporosis.Here we provide a comprehensive up-to-date review on the molecular signaling cascade of NELL-1 in mesenchymal stem cells and potential applications in bone regenerative engineering. | Mikhail Pakvasa Alex Alverdy Sami Mostafa Eric Wang Lucy Fu Alexander Li Leonardo Oliveira Aravind Athiviraham Michael J.Lee Jennifer Moriatis Wolf Tong-Chuan He Guillermo A.Ameer Russell R.Reid | 2017 | Genes & Diseases2017，4，3： | 13 |
| 2 | 3-D bioprinting technologies in tissue engineering and regenerative medicine:Current and future trends显示文摘Advances in three-dimensional(3D)printing have increased feasibility towards the synthesis of living tissues.Known as 3D bioprinting,this technology involves the precise layering of cells,biologic scaffolds,and growth factors with the goal of creating bioidentical tissue for a variety of uses.Early successes have demonstrated distinct advantages over conventional tissue engineering strategies.Not surprisingly,there are current challenges to address before 3D bioprinting becomes clinically relevant.Here we provide an overview of 3D bioprinting technology and discuss key advances,clinical applications,and current limitations.While 3D bioprinting is a relatively novel tissue engineering strategy,it holds great potential to play a key role in personalized medicine. | Elliot S.Bishop Sami Mostafa Mikhail Pakvasa Hue H.Luu Michael J.Lee Jennifer Moriatis Wolf Guillermo A.Ameer Tong-Chuan He Russell R.Reid | 2017 | Genes & Diseases2017，4，4： | 10 |
| 3 | Stem cell therapy for chronic skin wounds in the era of personalized medicine:From bench to bedside显示文摘With the significant financial burden of chronic cutaneous wounds on the healthcare system,not to the personal burden mention on those individuals afflicted,it has become increasingly essential to improve our clinical treatments.This requires the translation of the most recent benchtop approaches to clinical wound repair as our current treatment modalities have proven insufficient.The most promising potential treatment options rely on stem cellbased therapies.Stem cell proliferation and signaling play crucial roles in every phase of the wound healing process and chronic wounds are often associated with impaired stem cell function.Clinical approaches involving stem cells could thus be utilized in some cases to improve a body’s inhibited healing capacity.We aim to present the laboratory research behind the mechanisms and effects of this technology as well as current clinical trials which showcase their therapeutic potential.Given the current problems and complications presented by chronic wounds,we hope to show that developing the clinical applications of stem cell therapies is the rational next step in improving wound care. | Elam Coalson Elliot Bishop Wei Liu Yixiao Feng Mia Spezia Bo Liu Yi Shen Di Wu Scott Du Alexander J.Li Zhenyu Ye Ling Zhao Daigui Cao Alissa Li Ofir Hagag Alison Deng Winny Liu Mingyang Li Rex C.Haydon Lewis Shi Aravind Athiviraham Michael J.Lee Jennifer Moriatis Wolf Guillermo A.Ameer Tong-Chuan He Russell R.Reid | 2019 | Genes & Diseases2019，6，4： | 6 |
| 4 | Single capillary oximetry and tissue ultrastructural sensing by dual-band dual-scan inverse spectroscopic optical coherence tomography显示文摘Measuring capillary oxygenation and the surrounding ultrastructure can allow one to monitor a microvascular niche and better understand crucial biological mechanisms.However,capillary oximetry and pericapillary ultrastructure are challenging to measure in vivo.Here we demonstrate a novel optical imaging system,dual-band dual-scan inverse spectroscopic optical coherence tomography(D2-ISOCT),that,for the first time,can simultaneously obtain the following metrics in vivo using endogenous contrast:(1)capillary-level oxygen saturation and arteriolar-level blood flow rates,oxygen delivery rates,and oxygen metabolic rates;(2)spatial characteristics of tissue structures at length scales down to 30 nm;and(3)morphological images up to 2mm in depth.To illustrate the capabilities of D2-ISOCT,we monitored alterations to capillaries and the surrounding pericapillary tissue(tissue between the capillaries)in the healing response of a mouse ear wound model.The obtained microvascular and ultrastructural metrics corroborated well with each other,showing the promise of D2-ISOCT for becoming a powerful new non-invasive imaging tool. | Rongrong Liu James A.Winkelmann Graham Spicer Yunxiao Zhu Aya Eid Guillermo A.Ameer Vadim Backman Ji Yi | 2018 | Light(Science & Applications)2018，7，1： | 4 |
| 5 | Stem cells, growth factors and scaffolds in craniofacial regenerative medicine显示文摘Current reconstructive approaches to large craniofacial skeletal defects are often complicated and challenging.Critical-sized defects are unable to heal via natural regenerative processes and require surgical intervention,traditionally involving autologous bone(mainly in the form of nonvascularized grafts)or alloplasts.Autologous bone grafts remain the gold standard of care in spite of the associated risk of donor site morbidity.Tissue engineering approaches represent a promising alternative that would serve to facilitate bone regeneration even in large craniofacial skeletal defects.This strategy has been tested in a myriad of iterations by utilizing a variety of osteoconductive scaffold materials,osteoblastic stem cells,as well as osteoinductive growth factors and small molecules.One of the major challenges facing tissue engineers is creating a scaffold fulfilling the properties necessary for controlled bone regeneration.These properties include osteoconduction,osteoinduction,biocompatibility,biodegradability,vascularization,and progenitor cell retention.This review will provide an overview of how optimization of the aforementioned scaffold parameters facilitates bone regenerative capabilities as well as a discussion of common osteoconductive scaffold materials. | Viktor Tollemar Zach J.Collier Maryam K.Mohammed Michael J.Lee Guillermo A.Ameer Russell R.Reid | 2016 | Genes & Diseases2016，3，1： | 3 |
| 6 | Growth factor release from a chemically modified elastomeric poly(1,8‐octanediol‐co‐citrate) thin film promotes angiogenesis in vivo显示文摘 | Arun K.Sharma Matthew I.Bury Natalie J.Fuller Dorota I.Rozkiewicz Partha V.Hota David M.Kollhoff Matthew J.Webber NatalieTapaskar Jay W.Meisner Patrick J.Lariviere SamanthaDeStefano DeliWang Guillermo A.Ameer Earl Y.Cheng | 2012 | J Biomed Mater Res2012，，3： | 1 |
| 7 | Bone morphogenetic protein-9 effectively induces osteogenic differentiation of reversibly immortalized calvarial mesenchymal progenitor cells显示文摘Critical-sized craniofacial defect repair represents a significant challenge to reconstructive surgeons.Many strategies have been employed in an effort to achieve both a functionally and cosmetically acceptable outcome.Bone morphogenetic proteins(BMPs)provide a robust osteoinductive cue to stimulate bony growth and remodeling.Previous studies have suggested that the BMP-9 isoform is particularly effective in promoting osteogenic differentiation of mesenchymal progenitor cells.The aim of this study is to characterize the osteogenic capacity of BMP-9 on calvarial mesenchymal progenitor cell differentiation.Reversibly immortalized murine calvarial progenitor cells(iCALs)were infected with adenoviral vectors encoding BMP-9 or GFP and assessed for early and late stages of osteogenic differentiation in vitro and for osteogenic differentiation via in vivo stem cell implantation studies.Significant elevations in alkaline phosphatase(ALP)activity,osteocalcin(OCN)mRNA transcription,osteopontin(OPN)protein expression,and matrix mineralization were detected in BMP-treated cells compared to control.Specifically,ALP activity was elevated on days 3,7,9,11,and 13 post-infection and OCN mRNA expression was elevated on days 8,10,and 14 in treated cells.Additionally,treatment groups demonstrated increased OPN protein expression on day 10 and matrix mineralization on day 14 post-infection relative to control groups.BMP-9 also facilitated the formation of new bone in vivo as detailed by gross,microcomputed tomography,and histological analyses.Therefore,we concluded that BMP-9 significantly stimulates osteogenic differentiation in iCALs,and should be considered an effective agent for calvarial tissue regeneration. | Chad M.Teven Michael T.Rossi Deana S.Shenaq Guillermo A.Ameer Russell R.Reid | 2015 | Genes & Diseases2015，2，3： | 1 |
| 8 | Bioresorbable Multilayer Photonic Cavities as Temporary Implants for Tether-Free Measurements of Regional Tissue Temperatures显示文摘Objective and Impact Statement.Real-time monitoring of the temperatures of regional tissue microenvironments can serve as the diagnostic basis for treating various health conditions and diseases.Introduction.Traditional thermal sensors allow measurements at surfaces or at near-surface regions of the skin or of certain body cavities.Evaluations at depth require implanted devices connected to external readout electronics via physical interfaces that lead to risks for infection and movement constraints for the patient.Also,surgical extraction procedures after a period of need can introduce additional risks and costs.Methods.Here,we report a wireless,bioresorbable class of temperature sensor that exploits multilayer photonic cavities,for continuous optical measurements of regional,deep-tissue microenvironments over a timeframe of interest followed by complete clearance via natural body processes.Results.The designs decouple the influence of detection angle from temperature on the reflection spectra,to enable high accuracy in sensing,as supported by in vitro experiments and optical simulations.Studies with devices implanted into subcutaneous tissues of both awake,freely moving and asleep animal models illustrate the applicability of this technology for in vivo measurements.Conclusion.The results demonstrate the use of bioresorbable materials in advanced photonic structures with unique capabilities in tracking of thermal signatures of tissue microenvironments,with potential relevance to human healthcare. | Wubin Bai Masahiro Irie Zhonghe Liu Haiwen Luan Daniel Franklin Khizar Nandoliya Hexia Guo Hao Zang Yang Weng Di Lu Di Wu Yixin Wu Joseph Song Mengdi Han Enming Song Yiyuan Yang Xuexian Chen Hangbo Zhao Wei Lu Giuditta Monti Iwona Stepien Irawati Kandela Chad R.Haney Changsheng Wu Sang Min Won Hanjun Ryu Alina Rwei Haixu Shen Jihye Kim Hong-Joon Yoon Wei Ouyang Yihan Liu Emily Suen Huang-yu Chen Jerry Okina Jushen Liang Yonggang Huang Guillermo A.Ameer Weidong Zhou John A.Rogers | 2021 | Biomedical Engineering Frontiers2021，2，1： | 0 |
| 9 | Reversibly immortalized keratinocytes(iKera)facilitate re-epithelization and skin wound healing:Potential applications in cell-based skin tissue engineering显示文摘Skin injury is repaired through a multi-phase wound healing process of tissue granulation and re-epithelialization.Any failure in the healing process may lead to chronic non-healing wounds or abnormal scar formation.Although significant progress has been made in developing novel scaffolds and/or cell-based therapeutic strategies to promote wound healing,effective management of large chronic skin wounds remains a clinical challenge.Keratinocytes are critical to re-epithelialization and wound healing.Here,we investigated whether exogenous keratinocytes,in combination with a citrate-based scaffold,enhanced skin wound healing.We first established reversibly immortalized mouse keratinocytes(iKera),and confirmed that the iKera cells expressed keratinocyte markers,and were responsive to UVB treatment,and were non-tumorigenic.In a proof-of-principle experiment,we demonstrated that iKera cells embedded in citrate-based scaffold PPCN provided more effective re-epithelialization and cutaneous wound healing than that of either PPCN or iKera cells alone,in a mouse skin wound model.Thus,these results demonstrate that iKera cells may serve as a valuable skin epithelial source when,combining with appropriate biocompatible scaffolds,to investigate cutaneous wound healing and skin regeneration. | Jiamin Zhong Hao Wang Ke Yang Huifeng Wang Chongwen Duan Na Ni Liqin An Yetao Luo Piao Zhao Yannian Gou Shiyan Sheng Deyao Shi Connie Chen William Wagstaff b Bryce Hendren-Santiago b Rex C.Haydon b Hue H.Luu b Russell R.Reid Sherwin HHo Guillermo A.Ameer Le Shen Tong-Chuan He Jiaming Fan | 2022 | Bioactive Materials2022，7，3： | 0 |
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