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| 1 | Transplantation of collagen scaffold with autologous bone marrow mononuclear cells promotes functional endometrium reconstruction via downregulating ΔNp63 expression in Asherman's syndrome显示文摘Asherman's syndrome(AS) is a common disease that presents endometrial regeneration disorder. However, little is known about its molecular features of this aregenerative endometrium in AS and how to reconstruct the functioning endometrium for the patients with AS. Here, we report that ΔNp63 is significantly upregulated in residual epithelial cells of the impaired endometrium in AS; the upregulated-ΔNp63 induces endometrial quiescence and alteration of stemness. Importantly, we demonstrate that engrafting high density of autologous bone marrow mononuclear cells(BMNCs) loaded in collagen scaffold onto the uterine lining of patients with AS downregulates ΔNp63 expression, reverses ΔNp63-induced pathological changes, normalizes the stemness alterations and restores endometrial regeneration. Finally, five patients achieved successful pregnancies and live births. Therefore, we conclude that ΔNp63 is a crucial therapeutic target for AS. This novel treatment significantly improves the outcome for the patients with severe AS. | Guangfeng Zhao Yun Cao Xianghong Zhu Xiaoqiu Tang Lijun Ding Haixiang Sun Juan Li Xinan Li Chenyan Dai Tong Ru Hui Zhu Jingjie Lu Caimei Lin Jingmei Wang Guijun Yan Huiyan Wang Lei Wang Yimin Dai Bin Wang Ruotian Li Jianwu Dai Yan Zhou Yali Hu | 2017 | Science China(Life Sciences)2017，60，4： | 39 |
| 2 | Monitoring Maize Lodging Grades via Unmanned Aerial Vehicle Multispectral Image显示文摘Lodging is one of the main factors affecting the quality and yield of crops.Timely and accurate determination of crop lodging grade is of great significance for the quantitative and objective evaluation of yield losses.The purpose of this study was to analyze the monitoring ability of a multispectral image obtained by an unmanned aerial vehicle(UAV)for determination of the maize lodging grade.A multispectral Parrot Sequoia camera is specially designed for agricultural applications and provides new information that is useful in agricultural decision-making.Indeed,a near-infrared image which cannot be seen with the naked eye can be used to make a highly precise diagnosis of the vegetation condition.The images obtained constitute a highly effective tool for analyzing plant health.Maize samples with different lodging grades were obtained by visual interpretation,and the spectral reflectance,texture feature parameters,and vegetation indices of the training samples were extracted.Different feature transformations were performed,texture features and vegetation indices were combined,and various feature images were classified by maximum likelihood classification(MLC)to extract four lodging grades.Classification accuracy was evaluated using a confusion matrix based on the verification samples,and the features suitable for monitoring the maize lodging grade were screened.The results showed that compared with a multispectral image,the principal components,texture features,and combination of texture features and vegetation indices were improved by varying degrees.The overall accuracy of the combination of texture features and vegetation indices is 86.61%,and the Kappa coefficient is 0.8327,which is higher than that of other features.Therefore,the classification result based on the feature combinations of the UAV multispectral image is useful for monitoring of maize lodging grades. | Qian Sun Lin Sun Meiyan Shu Xiaohe Gu Guijun Yang Longfei Zhou | 2019 | Plant Phenomics2019，1，1： | 5 |
| 3 | Accounting for the China-US Trade Imbalance : An Ownership-Based Approach 显示文摘 | Yiping Xn Guijun Lin Huayu Sun | 2010 | Re- view of International Economics2010，18，3： | 1 |
| 4 | Unbiased Quasi-regression显示文摘伪回归,由在计算机实验产生的问题激发了,主要加快评估的焦点。然而,它的理论性质是未经勘探的全身的联盟者。这篇论文证明伪回归是不偏的,为参数评价而是它的强壮的会聚的 andasymptotic 正常为试穿被偏导弯。而且,调用不偏的伪回归的一个新方法被建议。除了保留参数评价的 aboveasymptotic 行为,不偏的伪回归为曲线的试穿是不偏的。 | Guijun YANG Lu LIN Runchu ZHANG | 2007 | Chinese Annals of Mathematics,Series B2007，28，2： | 1 |
| 5 | SJES14110700175297显示文摘 | Jingcheng Zhang Lin yuan Ruiliang Pu Rebecca W. Loraamm Guijun Yang Jihua Wang | 2014 | Computers and Electronics in Agriculture2014，，： | 1 |
| 6 | Magnetic field calculation and dynamic behavior analysis of the permanent magnetic actuator 显示文摘 | Lin Xin Gao Guijun Cai Zhiyuan | 2000 | International symposium on discharges and Electrical Insulation in Vacuum2000，，6： | 1 |
| 7 | A fluorescent film sensor for high-performance detection of Listeria monocytogenes via vapor sampling显示文摘Foodborne Listeria monocytogenes poses serious threats to public health.Fast and sensitive detection of the pathogen at the point of contamination is thus crucial to halt the spread of bacteria-related diseases.Herein,we report for the first time a fluorescent film sensor to detect the biomarker of L.monocytogenes,3-hydroxy-2-butanone.The sensor demonstrated unprecedented sensing performance for the analyte with a detection limit lower than 0.05 mg/m^(3),response time less than 1 s,full reversibility,and excellent selectivity.Further study showed that the sensor can be used to monitor the growth of L.monocytogenes with much-improved sensitivity.The superior performance of the sensor is ascribed to the specific binding,efficient charge transfer emission,and porous adlayer structure of the specially designed sensing fluorophore-based film.Our work paves the way to develop a portable detector to meet the needs for on-site and real-time detection of foodborne pathogens. | Rongrong Huang Meiqi Li Dehui Lin Yangtao Shao Congdi Shang Qianqian Liu Guijun Liu Nan Li Rong Miao Haonan Peng Yanli Tang Yu Fang | 2023 | Aggregate2023，4，1： | 0 |
| 8 | Three-dimensional-printed collagen/chitosan/secretome derived from HUCMSCs scaffolds for efficient neural network reconstruction in canines with traumatic brain injury显示文摘The secretome secreted by stem cells and bioactive material has emerged as a promising therapeutic choice for traumatic brain injury(TBI).We aimed to determine the effect of 3D-printed collagen/chitosan/secretome derived from human umbilical cord blood mesenchymal stem cells scaffolds(3D-CC-ST)on the injured tissue regeneration process.3D-CC-ST was performed using 3D printing technology at a low temperature(20C),and the physical properties and degeneration rate were measured.The utilization of low temperature contributed to a higher cytocompatibility of fabricating porous 3D architectures that provide a homogeneous distribution of cells.Immediately after the establishment of the canine TBI model,3D-CC-ST and 3D-CC(3D-printed collagen/chitosan scaffolds)were implanted into the cavity of TBI.Following implantation of scaffolds,neurological examination and motor evoked potential detection were performed to analyze locomotor function recovery.Histological and immunofluorescence staining were performed to evaluate neuro-regeneration.The group treated with 3D-CC-ST had good performance of behavior functions.Implanting 3D-CC-ST significantly reduced the cavity area,facilitated the regeneration of nerve fibers and vessel reconstruction,and promoted endogenous neuronal differentiation and synapse formation after TBI.The implantation of 3D-CC-ST also markedly reduced cell apoptosis and regulated the level of systemic inflammatory factors after TBI. | Xiaoyin Liu Guijun Zhang Pan Wei Lin Zhong Yaxing Chen Jianyong Zhang Xuyi Chen Liangxue Zhou | 2022 | Regenerative Biomaterials2022，9，1： | 0 |
| 9 | Changes of cerebral blood flow in rats with acute cerebral ischemia and the effect of nitric oxide donor S-nitroso-N-acetyl-penicillamine显示文摘BACKGROUND: Previous studies show that nitric oxide donor can increase cerebral blood flow and improve the function of neurons in cerebral ischemia, but the change does not happen in all the models of cerebral ischemia. OBJECTIVE: To observe the effects of nitric oxide donor S-nitroso-N-acetyl-penicillamine (SNAP) on the cerebral blood flow, cyclic guanosine monophosphate (cGMP) content in cerebral cortex, infarct volume and blood pressure in acute ischemic rat brain. DESIGN: A randomized and control animal experiment. SETTING: Department of Neurosurgery, Aerospace Central Hospital, Peking University. MATERIALS: Twenty-eight male Wistar rats of SPF grade, weighing 250-300 g, aged 10-12 weeks were randomly divided into control group (n =14) and SNAP-treated group (n =14). SNAP (5 mg/bottle) was provided by Beijing Chemical Reagent Company. Laser Doppler Flowmeter (FLO C1; Omegawave Inc., Tokyo, Japan) and immunoassay kit (Amersham Pharmacia Biotech, UK) were applied. METHODS: ① Model establishment: In the control group, models of cerebral ischemia were induced by ligating right common, internal and external carotid arteries; In the SNAP-treated group, models of cerebral ischemia were induced by ligating right common and external carotid arteries, followed by occluding middle cerebral artery and ligating internal carotid artery. ② Administration: In the SNAP-treated group, SNAP (100 μg/kg) was intravenously infused within 2 minutes, whereas in the control group, phosphate buffered saline (PBS, 1 mL) was intravenously infused (0.5 mL per minute). Six rats were used to measure the volume of cerebral infarction, and the other 8 rats were used to determine other indexes in each group respectively. ③ Determination of indexes: Regional cerebral blood flow (rCBF) was continuously measured by laser-Doppler flowmetry in the ischemic penumbra and contralateral cortex under the continuous monitoring of blood pressure. cGMP concentrations in brain tissue were determined using the enzyme immunoassay 20 minutes after administration. SNAP and PBS were infused in the SNAP-treated group and control group respectively at 10 minutes and 2 hours after ischemia, and the infarct volumes were estimated by 2,3,5-triphenyltetrazolium chloride staining. The differences of the measurement data were compared with the t test. MAIN OUTCOME MEASURES: The change of the cGMP and rCBF in acute ischemic rat brain were investigated and recorded after SNAP intravenous infusion. RESULTS: All the 28 rats were involved in the final analysis of results. ① rCBF: The rCBF in the ischemic side at the median part of the right frontoparietal cortex decreased to a similar extent between the control group and SNAP-treated group [(58.5±15.5)%, (53.0±11.2)%,t =1.345,P =0.10], which suggested that the severity of ischemia was comparative between the two groups. No significant change was observed in rCBF after the administration of PBS, and there was no obvious difference between the left and right cortices (t =0.896, P > 0.05). The rCBF in cortex of the ischemic side decreased at 15 and 20 minutes after administration of SNAP, and it was significantly different in the ischemic (right) and the contralateral (left) cortices (t =2.298, P =0.01; t =3.499, P < 0.01). ② cGMP levels in bilateral cortices: The cGMP levels in the ischemic and contralateral cortices in the SNAP-treated group were not significantly different [(163.02±40.7) and (162.47±43.98) pmol/g, P > 0.05], but were obviously higher than those in the control group [(105.86±29.4), (112.21±20.64) pmol/g, t =2.977, 2.560, P < 0.01]. ③ Volume of cerebral infarction: The infarction lesions were found mainly focused in the caudate nucleus and cerebral cortices in the right middle cerebral artery distribution field after 4 hours. The infarct volume in the control group was close to that in the SNAP-treated group [(123.35±55.08), (130.25±68.32) mm3, t =1.998, P < 0.05]. ④ Changes of blood pressure: The mean arterial blood pressure in the SNAP-treated group 5 minutes after SNAP infusion was obviously lower than that in the control group (t =1.985, P < 0.05). CONCLUSION: The intravenous infusion of SNAP therapy for cerebral ischemia can cause cGMP increase through acting in the upstream of the nitric oxide-cGMP reaction chain. SNAP did not decrease the volume of the cerebral infarction, and it did not protect the brain. However, SNAP can play a role in vasodilation and decompression. | Feng Gao Zhiqiang Yi Guijun Lin | 2006 | Neural Regeneration Research2006，1，5： | 0 |
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