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| 1 | Effect of casein glycomacropeptide on subunit p65 of nuclear transcription factor-B in lipopolysaccharide-stimulated human colorectal tumor HT-29 cells显示文摘Objective:The effect of casein glycomacropeptide(CGMP)on the expression of NF-B subunit p65 in human colorectal cancer HT-29 cells induced by lipopolysaccharide(LPS)was investigated to explore the therapeutic efficacy of CGMP for human colorectal cancer.Methods:HT-29 cells cultured in 96-well plates were stimulated with LPS for 30 min at the concentrations of 0,0.001,0.01,0.1,1 and 10g/mL.After stimulation,the expression of p65 in HT-29 cells was evaluated by immunofluorescence method.Similarly,HT-29 cells were incubated with 0,0.001,0.01,0.1,1,10 and 100g/mL CGMP for 24 h at the optimal LPS concentration.After treatment with CGMP under the incubation with optimal LPS concentration for 30 min,the protein expression of p65 was analyzed by Western blotting.The optimum culture time was evaluated by incubating HT-29 cells with the optimal CGMP concentration for 6,12,24,48 and 72 h.Results:NF-B-p65 revealed that the highest protein expression was achieved with 1g/mL LPS treatment.Meanwhile,CGMP could inhibit the protein expression of NF-B-p65 in LPS-stimulated HT-29 cells,and the optimal inhibitory effect was observed at a CGMP concentration of 0.01g/mL with 48 h incubation.Conclusion:CGMP can regulate NF-B signaling pathway through inhibiting the expression of its subunit p65,which is beneficial for the further improvement of human colorectal cancer treatment. | Jianmiao Gong Qingsen Chen Yali Yan Guangchang Pang | 2014 | Food Science and Human Wellness2014，3，2： | 5 |
| 2 | How functional foods play critical roles in human health显示文摘How do functional foods affect human health?To answer this question it is important to understand what happens when food is digested and taken up by the gastrointestinal(GI)tract.The gut is a selective nutrient absorption system and the most important signal transduction and information exchange system within the body.It acts as a signal transducer,a neuroendocrine sensor,and an immunological recognition and presentation system.It is also a complex information exchange system comprising a number of signaling networks involving GI cells and cells immobilized in organs or transported in blood.The bioactivity of functional foods in vivo may be due to their effects on such networks,but this raises the question of what signaling pathways are used by non-nutrients that cannot be absorbed by the gut.The purpose of this review is to describe intestinal nutrient transportation,identify a number of widely expressed receptors and signal transduction pathways,and outline our current understanding of the mechanisms involved in health and disease.At the end of the review,a method for developing a cell communication network is described.This network is convenient for investigating the effects of oral administration of experimental medicines,drugs,or functional foods on cytokines of interest.Because cytokines and chemokines are transported via the circulatory system,a simple 2–3 mL blood sample from a volunteer is a rich source of information.This method may become the gold standard for evaluating the effects of functional foods or medicines in vivo.©2012 Production and hosting by Elsevier B.V.on behalf of Beijing Academy of Food Sciences. | Guangchang Pang Junbo Xie Qingsen Chen Zhihe Hu | 2012 | Food Science and Human Wellness2012，1，1： | 4 |
| 3 | A novel pungency biosensor prepared with fixing taste-bud tissue of rats显示文摘 | QIAO Lixin JIAO Lihua PANG Guangchang | 2015 | Biosensors and Bioelectronics2015，68，： | 1 |
| 4 | Mg,Ti-base surface integrated layer and bulk doping to suppress lattice oxygen evolution of Ni-rich cathode material at a high cut-off voltage显示文摘The Nickel-rich layered cathode materials charged to 4.5 V can obtain a specific capacity of more than 200 m Ah g^(-1).However,the nickel-rich layered cathode materials suffer from the severe capacity fade during high-voltage cycling,which is related to the phase transformation and the surface sides reactions caused by the lattice oxygen evolution.Here,the simultaneous construction of a Mg,Ti-based surface integrated layer and bulk doping through Mg,Ti surface treatment could suppress the lattice oxygen evolution of Nirich material at deep charging.More importantly,Mg and Ti are co-doped into the particles surface to form an Mg_(2)TiO_(4) and Mg_(0.5–x)Ti_(2–y)(PO_(4))_(3) outer layer with Mg and Ti vacancies.In the constructed surface integrated layer,the reverse electric field in the Mg_(2)TiO_(4) effectively suppressed the outward migration of the lattice oxygen anions,while Mg_(0.5–x)Ti_(2–y)(PO_(4))_(3) outer layer with high electronic conductivity and good lithium ion conductor could effectively maintained the stability of the reaction interface during highvoltage cycling.Meanwhile,bulk Mg and Ti co-doping can mitigate the migration of Ni ions in the bulk to keep the stability of transition metal–oxygen(M-O)bond at deep charging.As a result,the NCM@MTP cathode shows excellent long cycle stability at high-voltage charging,which keep high capacity retention of 89.3%and 84.3%at 1 C after 200 and 100 cycles under room and elevated temperature of 25 and 55°C,respectively.This work provides new insights for manipulating the surface chemistry of electrode materials to suppress the lattice oxygen evolution at high charging voltage. | Fan Peng Youqi Chu Yu Li Qichang Pan Guangchang Yang Lixuan Zhang Sijiang Hu Fenghua Zheng Hongqiang Wang Qingyu Li | 2022 | Journal of Energy Chemistry2022，31，8： | 1 |
| 5 | Genomes of 12 fig wasps provide insights into the adaptation of pollinators to fig syconia显示文摘Figs and fig pollinators are one of the few classic textbook examples of obligate pollination mutualism.The specific dependence of fig pollinators on the relatively safe living environment with sufficient food sources in the enclosed fig syconia implies that they are vulnerable to habitat changes.However,there is still no extensive genomic evidence to reveal the evolutionary footprint of this long-term mutually beneficial symbiosis in fig pollinators.In fig syconia,there are also non-pollinator species.The non-pollinator species differ in their evolutionary and life histories from pollinators.We conducted comparative analyses on 11 newly sequenced fig wasp genomes and one previously published genome.The pollinators colonized the figs approximately 66.9 million years ago,consistent with the origin of host figs.Compared with nonpollinators,many more genes in pollinators were subject to relaxed selection.Seven genes were absent in pollinators in response to environmental stress and immune activation.Pollinators had more streamlined gene repertoires in the innate immune system,chemosensory toolbox,and detoxification system.Our results provide genomic evidence for the differentiation between pollinators and nonpollinators.The data suggest that owing to the long-term adaptation to the fig,some genes related to functions no longer required are absent in pollinators. | Jinhua Xiao Xianqin Wei Yi Zhou Zhaozhe Xin Yunheng Miao Hongxia Hou Jiaxing Li Dan Zhao Jing Liu Rui Chen Liming Niu Guangchang Ma Wenquan Zhen Shunmin He Jianxia Wang Xunfan Wei Weihao Dou Zhuoxiao Sui Haikuan Zhang Shilai Xing Miao Shi Dawei Huang | 2021 | Journal of Genetics and Genomics2021，48，3： | 1 |
| 6 | Effects of casein glycomacropeptide on the early development of primary colorectal cancer in rats显示文摘In this paper,we utilized dimethylhydrazine(DMH)-induced colorectal cancer(CC)model rats to explore the effects of casein glycomacropeptide(CGMP)on colorectal cancer.Rats with CC were orally administrated with 10,50,or 100 mg/kg bw d CGMP,or the same volume of phosphatebuffered saline for 15 weeks.The total numbers of aberrant crypt foci(ACF)and crypts per focus in colon were scored using a light microscope at low magnification after the colon was stained with methylene blue solutions.The methylation level of DNA extracted from colon was detected using methylation-specific PCR.The expression of p16 and mucin 2(MUC2)proteins were measured by immunohistochemistry.The results showed that although ACF were found in rats treated with CGMP,their number was significantly decreased compared to that of model rats.In addition,methylation and expression levels of p16 and MUC2 were also inhibited by CGMP,which were more obvious in rats treated with 50 mg/kg bw d CGMP.In conclusion,CGMP has potential application as nutritional therapy for preventing colorectal cancer.©2013 Beijing Academy of Food Sciences.Production and hosting by Elsevier B.V.All rights reserved. | Qingsen Chen Yun Liang Chenchen Zhu Yali Yan Guangchang Pang | 2013 | Food Science and Human Wellness2013，2，3： | 1 |
| 7 | Effects of the polysaccharide from Pholiota nameko on human cytokine network in serum显示文摘 | Haiping Li Xiaojuan Liu Yang Li Yanjun Hua Dexian Zhi Guangchang Pang | 2011 | International Journal of Biological Macromolecules2011，，1： | 1 |
| 8 | Modulation effect of Semen Ziziphi Spinosae extracts on IL-1β, IL-4, IL-6, IL-10, TNF-α and IFN-γ in mouse serum显示文摘 | Junbo Xie Li Guo Guangchang Pang Xin Wu Mingchun Zhang | 2011 | Natural Product Research2011，，4： | 1 |
| 9 | Fabrication of Bacillus cereus electrochemical immunosensor based on double-layer gold nanoparticles and chitosan显示文摘 | KANG Xiaobin PANG Guangchang CHEN Qingsen | 2013 | Sensors and Actuators B2013，177，： | 1 |
| 10 | Remodeling of the ryanodine receptor isoform 1 channel regulates the sweet and umami taste perception of Rattus norvegicus显示文摘Sweet and umami tastes are elicited by sweet and umami receptors on the tongue and palate epithelium,respectively.However,the molecular machinery allowing the taste reaction remains incompletely understood.Through a phosphoproteomic approach,we identified the key proteins that trigger taste mechanisms based on phosphorylation cascades.Ryanodine receptor isoform 1(RYR1)was further verified by sensory and behavioral assays.We propose a model of RYR1-mediated sweet/umami signaling in which the RYR1 channel,which mediates Ca^(2+)release from the endoplasmic reticulum,is closed by dephosphorylation in bud tissue after sweet/umami treatment.The alteration in Ca^(2+)content in the cytosol induces transient membrane depolarization and generates a cell current for taste signal transduction.We demonstrate that RYR1 is a new channel involved in the regulation of sweet/umami signal transduction and propose a“metabolic clock”notion based on sweet/umami sensing.Our study provides a valuable foundation for a system-level understanding of the taste perception mechanism. | Wenli Wang Dingqiang Lu Qiuda Xu Yulian Jin Guangchang Pang Yuan Liu | 2023 | Fundamental Research2023，3，3： | 1 |
| 11 | Energy intake,metabolic homeostasis,and human health显示文摘The energy substances(mainly carbohydrates and fats)are the basis and guarantee of life activity,especially the oxidative phosphorylation for energy supply.However,excessive absorption and accumulation of these substances can lead to metabolic diseases such as obesity,hyperlipidemia,diabetes,and cancers.A large amount of studies demonstrate that G protein-coupled receptors(GPCRs)play a key role in identification and absorption of energy substances,and the signaling network of nerves,immune,and endocrine regulates their storage and utilization.The gastrointestinal mucus layer not only identifies these substances through identification in diet components but also transfers immune,metabolic,and endocrine signals of hormones,cytokines,and chemokines by promoting interactions between receptors and ligands.These signaling molecules are transferred to corresponding organs,tissues,and cells by the circulatory system,and cell activity is regulated by amplifying of cell signals that constitute the wireless communication network among cells in the body.Absorption,accumulation,and utilization of energy substances in the body obey the law of energy conservation.Energy is stored in the form of fat,and meets the demand of body via two coupled mechanisms:catabolism and oxidative phosphorylation.Under normal physiological conditions,fat consumption involves ketone body metabolism through the circulatory system and glucose consumption requires blood lactic acid cycle.Accumulation of excessive energy leads to the abnormal activation of mammalian target of rapamycin(mTOR),thus promoting the excretion of glucose or glycogen in the form of blood glucose and urine glucose.Alternatively,the body cancels the intercellular contact inhibition and promotes cell proliferation to induce carcinogenesis,which can induce the consumption of large amounts of glucose.Intercellular communication is performed by signaling molecules via sensing,absorption,accumulation,and utilization of energy substances,and anabolism and catabolism are controlled by the central metabolic pathway.Therefore,slower catabolism will result in longer life expectancy,whereas faster catabolism results in shorter life expectancy.Energy substances in diet influence the balance between energy and metabolism in the body through the sensing function of the gastrointestinal system at two levels:cellular communication network and metabolic network.The present review of studies aims to strengthen our knowledge on cellular communication and metabolic networks to offer a dietary guidance on the metabolism and communication role of various foods. | Guangchang Pang Junbo Xie Qingsen Chen Zhihe Hu | 2014 | Food Science and Human Wellness2014，3，3： | 0 |
| 12 | An explicit fairing indicator for 2D curves显示文摘Resorting to cubic spline function instead of parametric spline representation,an explicit fairness indicator and an efficient fairing algorithm for 2D curves are presented.The input point sequence is firstly partitioned into several overlapped function segments.For each segment,a cubic spline function is used as the representation tool which entails a polyline approximation of curvature plot.Based on the extrinsic relationship between the polyline and the positions of data points,a coarse-to-fine faring method is proposed which efficiently identifies and eliminates the unnecessary inflection points.Our algorithm generates the best results to date,which is validated by numerous practical examples. | Han Xiaoguang Zhang Qing Dong Guangchang Liu Ligang | 2016 | Computer Aided Drafting,Design and Manufacturing2016，26，1： | 0 |
| 13 | WiFi6 Dynamic Channel Optimization Method for Fault Tolerance in Power Communication Network显示文摘As the scale of power networks has expanded,the demand for multi-service transmission has gradually increased.The emergence of WiFi6 has improved the transmission efficiency and resource utilization of wireless networks.However,it still cannot cope with situations such as wireless access point(AP)failure.To solve this problem,this paper combines orthogonal fre-quency division multiple access(OFDMA)technology and dynamic channel optimization technology to design a fault-tolerant WiFi6 dynamic resource optimization method for achieving high quality wireless services in a wirelessly covered network even when an AP fails.First,under the premise of AP layout with strong coverage over the whole area,a faulty AP determination method based on beacon frames(BF)is designed.Then,the maximum signal-to-interference ratio(SINR)is used as the principle to select AP reconnection for the affected users.Finally,this paper designs a dynamic access selection model(DASM)for service frames of power Internet of Things(IoTs)and a schedul-ing access optimization model(SAO-MF)based on multi-frame transmission,which enables access optimization for differentiated services.For the above mechanisms,a heuristic resource allocation algorithm is proposed in SAO-MF.Simulation results show that the method can reduce the delay by 15%and improve the throughput by 55%,ensuring high-quality communication in power wireless networks. | Hong Zhu Lisha Gao Lei Wei Guangchang Yang Sujie Shao | 2023 | Computers, Materials & Continua2023，，6： | 0 |
| 14 | Seismic behavior of outrigger truss-wall shear connections using multiple steel angles显示文摘An experimental investigation on the seismic behavior of a type of outrigger truss-reinforced concrete wall shear connection using multiple steel angles is presented. Six large-scale shear connection models, which involved a portion of reinforced concrete wall and a shear tab welded onto a steel endplate with three steel angles, were constructed and tested under combined actions of cyclic axial load and eccentric shear. The effects of embedment lengths of steel angles, wall boundary elements, types of anchor plates, and thicknesses of endplates were investigated. The test results indicate that properly detailed connections exhibit desirable seismic behavior and fail due to the ductile fracture of steel angles. Wall boundary elements provide beneficial confinement to the concrete surrounding steel angles and thus increase the strength and stiffness of connections. Connections using whole anchor plates are prone to suffer concrete pry-out failure while connections with thin endplates have a relatively low strength and fail due to large inelastic deformations of the endplates. The current design equations proposed by Chinese Standard 04G362 and Code GB50011 significantly underestimate the capacities of the connection models. A revised design method to account for the influence of previously mentioned test parameters was developed. | Li Xian Wang Wei Lü Henglin Zhang Guangchang | 2016 | Earthquake Engineering and Engineering Vibration2016，15，2： | 0 |
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