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| 1 | Danggui-shaoyao-san, a traditional Chinese medicine prescription, alleviates the orthodontic pain and inhibits neuronal and microglia activation显示文摘 | Li Hongshi Gu Zexu Wu Li'an Xia Liang Zhou Kecheng E Lingling Wang Dongsheng Kou Junping Liu Hongchen | 2014 | Chinese Medical Journal2014，，20： | 4 |
| 2 | Osteogenic and antibacterial dual functions of a novel levofloxacin loaded mesoporous silica microspheres/nano-hydroxyapatite/ polyurethane composite scaffold显示文摘Lev/MSNs/n-HA/PU has been proved to be a novel scaffold material to treat bone defect caused by chronic osteomyelitis.We have previously identified that this material can effectively treat chronic osteomyelitis caused by Staphylococcus aureus in vivo.However,the potential mechanisms of antibacterial and osteogenic induction properties remain unclear.Thus,for osteogenesis property,immunohistochemistry,PCR,and Western blot were performed to detect the expression of osteogenic markers.Furthermore,flow cytometry and TUNEL were applied to analyze MC3T3-E1 proliferation and apoptosis.For antibacterial property,the material was co-cultivated with bacteria,bacterial colony forming units was counted and the release time of the effective levofloxacin was assayed by agar disc-diffusion test.Moreover,scanning electron microscope was applied to observe adhesion of bacteria.In terms of osteogenic induction,we found BMSCs adherently grew more prominently on Lev/MSNs/n-HA/PU.Lev/MSNs/n-HA/PU also enhanced the expression of osteogenic markers including OCN and COL1a1,as well as effectively promoted the transition from G1 phase to G2 phase.Furthermore,Lev/MSNs/n-HA/PU could reduce apoptosis of MC3T3-E1.Besides,both Lev/MSNs/n-HA/PU and n-HA/PU materials could inhibit bacterial colonies,while Lev/MSNs/n-HA/PU possessed a stronger antibacterial activities,and lower bacterial adhesion than n-HA/PU.These results illustrated that Lev/MSNs/n-HA/PU composite scaffold possess favorable compatibility in vitro,which induce osteogenic differentiation of MSCs,promote proliferation and differentiation of MC3T3-E1,and inhibit apoptosis.Moreover,clear in vitro antibacterial effect of Lev/MSNs/n-HA/PU was also observed.In summary,this study replenishes the potential of Lev/MSNs/n-HA/PU composite scaffold possess dual functions of anti-infection and enhanced osteogenesis for future clinical application. | Zhiping Kuang Guangming Dai Ruijie Wan Dongli Zhang Chen Zhao Cheng Chen Jidong Li Hongchen Gu Wei Huang | 2021 | Genes & Diseases2021，8，2： | 4 |
| 3 | Control of Aggregate Size of Polyethyleneimine-Coated Magnetic Nanoparticles for Magnetofection显示文摘Using magnetic nanoparticles to enhance gene transfection,a recently developed technique termed magnetofection,has been shown to be a powerful technology in gene delivery.The most widely used magnetic nanoparticles in this area are those coated with polyethyleneimine,which is a well known non-viral transfection agent.In this article,we report methods to control the aggregate size of polyethyleneimine-coated magnetite particles.These particles were then used to enhance transfection of green fluorescent protein(GFP)into NIH 3T3 cells in vitro.We find that the aggregate size of the particles has a great effect on their performance in magnetofection,with less aggregated magnetic particles being more effective in enhancing the gene transfection. | Xiaoliang Wang Linzhu Zhou Yongjie Ma Xu Li Hongchen Gu | 2009 | Nano Research2009，2，5： | 4 |
| 4 | Silver nanoparticles-decorated and mesoporous silica coated single-walled carbon nanotubes with an enhanced antibacterial activity for killing drug-resistant bacteria显示文摘The mounting threat of antibiotic-resistant bacterial infections has made it imperative to develop innovative antibacterial strategies.Here we propose a novel antibacterial nanoplatform of silver nanoparticles-decorated and mesoporous silica coated single-walled carbon nanotubes constructed via a N-[3-(trimethoxysiltyl)propyl]ethylene diamine(TSD)-mediated method(SWCNTs@mSiO2-TSD@Ag).In this system,the outer mesoporous silica shells are able to improve the dispersibility of SWCNTs,which will increase their contact area with bacteria cell walls.Meanwhile,the large number of mesopores in silica layers act as microreactors for in situ synthesis of Ag NPs with controlled small size and uniform distribution,which induces an enhanced antibacterial activity.Compared with TSD modified mesoporous silica coated single-walled carbon nanotubes(SWCNTs@mSiO2-TSD)and commercialAg NPs,this combination nanosystem of SWCNTs@mSiO2-TSD@Ag exhibits much stronger antibacterial performance against multi-drug-resistant bacteria Escherichia coli(E.coli)and Staphylococcus aureus(S.aureus)in vitro through damaging the bacterial cell membranes and a fast release of silver ions.Furthermore,the in vivo rat skin infection model verifies that SWCNTS@mSiO2-TSD@Ag have remarkably improved abilities of bacterial clearance,wound healing promoting as well as outstanding biocompatibility.Therefore,this novel nanoplatform indicates promising potentials as a safe and powerful tool for the treatment of clinical drug-resistant infections. | Yu Zhu Jia Xu Yanmao Wang Cang Chen Hongchen Gu Yimin Chai Yao Wang | 2020 | Nano Research2020，13，2： | 2 |
| 5 | Extraction, detection, and profiling of serum biomarkers using designed Fe3O4@SiO2@HA core-shell particles显示文摘 | Chandrababu Rejeeth Xuechao Pang Ru Zhang Wei Xu Xuming Sun Bin Liu Jiatao Lou Jingjing Wan Hongchen Gu Wei Yan Kun Qian | 2018 | Nano Research2018，11，1： | 2 |
| 6 | Enhancement of the efficiency of PEI/liposome transfection by magnetofectins formed via electrostatic self-assembly显示文摘One of the major challenges for successful gene therapy is improving the transfection efficiency of non-viral vectors. Magnetic nanoparticles (MNPs) have been developed as enhancers of non-viral vehicles. We prepared MNPs and modified them with polyethyleneimine (PEI), citric acid (CA) or carboxylmethyl-dextran (CMD). Both positively charged MNPs (MNPs@PEI) and negatively charged MNPs (MNPs@CA, MNPs@CMD) could spontaneously form transfection complexes (magnetofectins) with plasmid DNA and PEI/liposome via electrostatic self-assembly. Our results showed as-prepared magnetofectins apparently enhanced PEI/liposome transfection efficiency and/or gene expression level into COS-7 cells with reduced transfection time from 4 h to 15 min under a magnetic field in vitro. Meanwhile, the effect of magnetofection was cell line-dependant. These results suggest that charged MNPs could improve transfection efficiency for non-viral vectors by simply mixing with them and by exerting a magnetic force. Thus such MNPs provide a convenient platform for further applications of gene delivery. | MA YongJie WANG XiaoLiang GU HongChen | 2012 | Chinese Science Bulletin2012，57，31： | 2 |
| 7 | The interaction of GSSG modified magnetic nanoparticles with SPC-A1 cells in vitro显示文摘Magnetic nanoparticles(MNPs) are promising materials for various biomedical applications,including magnetic resonance imaging,stem cell tracking,gene/drug delivery,and cancer treatment.To increase the effectiveness of MNPs,high capture efficiency and controlled uptake of the particles by cells is required.In this paper we report the cytotoxicity and cellular uptake into SPC-A1 cells of oxidized glutathione(GSSG)-modified MNPs(GSSG@Fe3O4).Experimental findings indicated that GSSG@Fe3O4 were biocompatible,and could be efficiently taken up by SPC-A1 cells(up to 160 pg iron per cell).The internalized GSSG@Fe3O4 was retained in the cell cytoplasm for 6 generations.The uptake of GSSG@Fe3O4 into SPC-A1 cells was energy-,concentration-and time-dependent.Pinocytosis may be involved in the internalization process of GSSG@Fe3O4 into SPC-A1 cells,but this mechanism remains to be elucidated.The controlled and efficient localization of GSSG@Fe3O4 into the cytosol and long intracellular retention provides theoretical and experimental insight into the biomedical applications for these molecules. | MA YongJie YAN ZhuBing XU Hong GU HongChen | 2012 | Chinese Science Bulletin2012，57，26： | 2 |
| 8 | Magnetite fer- rofluid with high specific absorption rate for application in hyperthermia显示文摘 | Zhang Liying Gu Hongchen Wang Xuman | 2007 | Journal of Magnetism and Magnetic Mate- rials2007，311，： | 1 |
| 9 | Synthesis and kinetic shape and size evolution of magnetite nanoparticles显示文摘 | ZHANG Ling HE Rong GU Hongchen | 2006 | Materials Research Bulletin2006，41，： | 1 |
| 10 | Preparation of nanosized Mo powder by microwave plasma chemical vapor deposition method 显示文摘 | Gu Hongchen Chen Qiling | 1999 | Materials Chemistry and Physice1999，59，： | 1 |
| 11 | Glimepiride induces proliferation and differentiation of rat osteoblasts via the PI3-kinase/Akt pathway显示文摘 | Pan Ma Bin Gu Junli Ma Lingling E Xia Wu Junkai Cao Hongchen Liu | 2010 | Metabolism2010，，3： | 1 |
| 12 | Preparation and crystal- lization of poly ( ethylene ter ephthalate )/SiO2 nanocomposites by in-situ polymerization 显示文摘 | Yongzhe Yang Hong Xu Hongchen Gu | 2006 | Journal of Applied Polymer Seience2006，102，1： | 1 |
| 13 | Development of lateral flow immunoassay system based on superparamagnetic nanobeads as labels for rapid quantitative detection of cardiac troponin I显示文摘 | Xu Quanfu Xu Hong Gu Hongchen | 2009 | Materials Science and Engineering C2009，29，： | 1 |
| 14 | Dendrimer modified magnetite nanoparticles for protein immobilizeation显示文摘 | PAN Bifeng FENG Gao GU Hongchen | 2005 | Journal of Colloid and Interface Science2005，，284： | 1 |
| 15 | Development of lateral flow immunoassay system based on superparamagnetic nanobeads as labels for rapid quantitative detection of cardiac troponin I显示文摘 | QuanFu Xu Hong Xu Hongchen Gu JingBo Li Yanyan Wang Meng Wei | 2009 | Materials Science & Engineering C2009，，3： | 1 |
| 16 | Magnetic polymer nanospheres with high and uniform magnetite content显示文摘 | Gao Feng Gu Hongchen | 2005 | Journal of Magnetism and Magnetic Materials2005，288，： | 1 |
| 17 | Preparation of Nanosized Mo Powder by Microwave Plasma Chemical Vapot Deposition Method显示文摘 | Liu Binghai Gu Hongchen Chen Qiling | 1999 | Materials Chemistry and Physics1999，59，3： | 1 |
| 18 | Preparation of Nanosized Mo Powder by Microwave Plasma Chemical Vapor Deposition Method显示文摘 | Binghai Liu Hongchen Gu Qilin Chen | 1999 | Material Chemistry and Physics1999，59，3： | 1 |
| 19 | Magnetite Ferrofluid With High Specific Absorption Rate for Application in Hyperthermia 显示文摘 | Zhang Liying Gu Hongchen Wang Xuman | 2007 | Journal of Magnetism and Magnetic Materials2007，311，： | 1 |
| 20 | Dendrimer modified magnetite nanop articles for protein immobiliz-ation显示文摘 | Pan Bifeng Gao Feng Gu Hongchen | 2005 | Journal of Colloid and Interface Science2005，，284： | 1 |
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