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| 1 | Fabrication of PEI-Liposome Loaded Fish Gelatin Nanofibers Mats by Green Electrospinning显示文摘Polyethyleneimine( PEI) and cationic liposomes were widely used for gene delivery and the combination of PEI and liposome was reported to result in a higher efficiency of cell transfection in vitro.In recent years, better transfection was observed for the drug-loaded liposome fixed on the tissue engineering scaffolds via embedding,surface adsorption or covalent grafting thus protected and bound by the scaffolds.In the present study,a novel PEI-liposome loaded fish gelatin composite nanofiber was successfully fabricated by a green electrospinning process.The existence of PEIliposome in the composite nanofibers was determined by Fourier transform infrared( FTIR) spectra, transmission electron microscopy( TEM), and confocal laser scanning microscopy( CLSM).As shown by scanning electron microscopy( SEM),the electrospun composite nanofibers with uniform diameter were smooth and round,and the morphology of the fish gelatin fibers did not change significantly after the incorporation of PEI-liposomes.The transfection results in vitro suggest PEI-liposome loaded fish gelatin material may have a promising application in non-viral gene delivery systems. | 陈梦霞 林思 王红声 | 2014 | Journal of Donghua University(English Edition)2014，31，5： | 2 |
| 2 | Effect of Polyethylenimine Content on Liposome/Polyethylenimine Complexes-Mediated Gene Delivery显示文摘Cationic liposome(Lipo) and polyethylenimine(PEI) are widely applied for nonviral gene transfection.In this study,in order to combine the favorable properties of Lipo and PEI systems for gene delivery,Lipo/PEI complexes with different contents of PEI(5%,10%,20% and 40% relative to phosphatidyl choline in reaction system) were prepared.The physicochemical properties of Lipo/PEI complexes,as well as the influences of PEI content on the storage stability,cytotoxicity and transfection efficiency were investigated.The transmission electron microscopy(TEM) images showed that Lipo/PEI complexes had smaller size compared to pure Lipo.The zeta potential values decreased with the increasing content of PEI.After storaged for 3 months at 4 ℃,obvious aggregation was observed when the addition of PEI content was up to 20%.In vitro cytotoxicity assay showed that Lipo/PEI complexes had decreased cytotoxicity over pure PEI,while the cytotoxicity was enhanced as the PEI content increased.Importantly,the luciferase activity assay and confocal microscope observation revealed that Lipo/PEI complexes prepared with the lowest amount of PEI(Lipo/PEI-5%)possessed the highest transfection efficiency.Thus,these results suggest that feeding the appropriate content of PEI in Lipo/PEI complexes allows them to be excellent vehicle for gene delivery. | 周小军 秦明 陈良 聂伟 莫秀梅 何创龙 | 2017 | Journal of Donghua University(English Edition)2017，34，2： | 0 |
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