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| 1 | Development and optimization of thermal contrast amplification lateral flow immunoassays for ultrasensitive HIV p24 protein detection显示文摘Detection of human immunodeficiency virus(HIV)p24 protein at a single pg/ml concentration in point-of-care(POC)settings is important because it can facilitate acute HIV infection diagnosis with a detection sensitivity approaching that of laboratory-based assays.However,the limit of detection(LOD)of lateral flow immunoassays(LFAs),the most prominent POC diagnostic platform,falls short of that of laboratory protein detection methods such as enzyme-linked immunosorbent assay(ELISA).Here,we report the development and optimization of a thermal contrast amplification(TCA)LFA that will allow ultrasensitive detection of 8 pg/ml p24 protein spiked into human serum at POC,approaching the LOD of a laboratory test.To achieve this aim,we pursued several innovations as follows:(a)defining a new quantitative figure of merit for LFA design based on the specific to nonspecific binding ratio(BR);(b)using different sizes and shapes of gold nanoparticles(GNPs)in the systematic optimization of TCA LFA designs;and(c)exploring new laser wavelengths and power regimes for TCA LFA designs.First,we optimized the blocking buffer for the membrane and running buffer by quantitatively measuring the BR using a TCA reader.The TCA reader interprets the thermal signal(i.e.,temperature)of GNPs within the membrane when irradiated by a laser at the plasmon resonance wavelength of the particle.This process results in higher detection and quantitation of GNPs than in traditional visual detection(i.e.,color intensity).Further,we investigated the effect of laser power(30,100,200 mW),GNP size and shape(30 and 100 nm gold spheres,150 nm gold-silica shells),and laser wavelength(532,800 nm).Applying these innovations to a new TCA LFA design,we demonstrated that 100nm spheres with a 100 mW 532 nm laser provided the best performance(i.e.,LOD=8pg/ml).This LOD is significantly better than that of the current colorimetric LFA and is in the range of the laboratory-based p24 ELISA.In summary,this TCA LFA for p24 protein shows promise for detecting acute HIV infection in POC settings. | Li Zhan Timothy Granade Yilin Liu Xierong Wei Ae Youngpairoj Vickie Sullivan Jeff Johnson John Bischof | 2020 | Microsystems & Nanoengineering2020，6，1： | 3 |
| 2 | Chemical investigations of the toxins produced by marine dinoflagellates显示文摘 | Schantz Granade H R | 1996 | Biochemisty1996，5，： | 1 |
| 3 | Characterizing the factor structure of parent reported executive function in autism spectrum disorders:the impact of cognitive inflexibility 显示文摘 | Granader Y Wallace GL Hardy KK | 2014 | J Autism Dev Disord2014，44，12： | 1 |
| 4 | Evaluation of rapid pre- natal human inmmnodeficiency virus testing in rural Cameroon 显示文摘 | Granade TC Parekh BS Tih PM | 2005 | Clin Diagn Lab hnmunol2005，12，7： | 1 |
| 5 | Evaluation of rapid prenatal human immunodeficiency virus testing in rural Cameroon显示文摘 | Granade TC Parekh BS Tih PM | 2005 | ClinDiagn Lab Immunol2005，12，7： | 1 |
| 6 | Modification of Rapid Human Immunodeficiency Virus(HIV)Antibody Assay Protocols for Detecting Recent HIV Seroconversion显示文摘 | Stephen D Soroka Timothy C Granade Debra Candal | 2005 | Clinical and Diagnostic Laboratory Immunology2005，8，： | 1 |
| 7 | Performance of theOraQuick and Hema-Strip rapid HIV antibody detection assaysby non-laboratorian显示文摘 | Granade TC Perekh BS Philips SK | 2004 | J Clin Virol2004，30，3： | 1 |
| 8 | Bench- and field-scale evaluation of chromium and cadmium extraction by electrokinetics显示文摘 | David B Gent R.Mark Bricka Akram N Alshawabkeh Steven L Larson Gene Fabian Steve Granade | 2004 | Journal of Hazardous Materials2004，，1： | 1 |
| 9 | MRI and mammography surveillance of women at increased risk for breast cancer: recommendations using an evidence - based approach 显示文摘 | Granader E J Dwamena B Carlos R C | 2008 | Acad Radiol2008，15，12： | 1 |
| 10 | Rapid detection of HIV-1 p24 antigen using magnetic immuno-chromatography (MICT)显示文摘 | Shon Workman Susan K. Wells Chou-Pong Pau S. Michele Owen X. Fan Dong Ron LaBorde Timothy C. Granade | 2009 | Journal of Virological Methods2009，，1： | 1 |
| 11 | Ciguatera I: brine shrimp (Artemia salina L) larval assay for ciguatera toxins显示文摘 | GRANADE H R CHENG P C DOORENBOS N J | 1976 | J Pharm Sci1976，65，： | 1 |
| 12 | Field evaluation of rapid HIV serologic tests for screening and confirming HIV-1 infection in Honduras显示文摘 | Stetler H C Granade T C Nunez C A | 1997 | AIDS1997，11，： | 1 |
| 13 | Liquid chromatographicmass spectrometric methods for the determination of marine polyether toxins 显示文摘 | DICKEY R W BENCSATH F A GRANADE H R | 1992 | Bull Soc Pathol Experiment1992，85，： | 1 |
| 14 | Scientific research activity and communication measured with cybermetrics indicators 显示文摘 | Aguillo I F Granad ino B Ortega J L | 2006 | Journal of the American Society for Information Science and Technology2006，57，10： | 1 |
| 15 | All-Optical Production of a Degenerate Fermi Gas 显示文摘 | GRANADE S R GEHM M E THOMAS J E | 2002 | Phys Rev Lett2002，88，12： | 1 |
| 16 | Enhancing emo- tion recognition in children with autism spectrum condi- tions: An intervention using animated vehicles with real emotional faces显示文摘 | Golan O Ashwin E Granader Y | 2010 | Journal of Autism and Developmental Disorder2010，40，3： | 1 |
| 17 | Field evaluation of rapid HIV serologic tests for screening and confirming HIV-1 infection in Honduras显示文摘 | Stetler H C Granade T C Nunez C A Meza R Terrell S Amador L George J R | | 0，，03： | 1 |
| 18 | Enhancing emotion recognition in children with autism spectrum conditions:an intervention using animated vehicles with real emotional faces显示文摘 | Golan O Ashwin E Granader Y | 2010 | J Autism Dev Disord2010，40，3： | 1 |
| 19 | Enhancing Emotion Recognition in Children with Autism Spectrum Conditions:An Intervention Using Animated Vehicles with Real Emotional Faces显示文摘 | Golan O Ashwin E Granader Y | | 0，，03： | 1 |
| 20 | 查看详情显示文摘 | K M O'Hara S L IIemmer M E Gehm S R Granade and J E Thomas | | 0，，5601： | 1 |
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