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| 1 | Oxidative stress in humans following the Pringle manoeuvre显示文摘BACKGROUND: Oxidative stress is induced in the liver by application of the Pringle manoeuvre. Malondialdehyde is a carbonyl compound formed during lipid peroxidation and prostaglandin biosynthesis, which combines with DNA to form a number of adducts. Among them is the DNA ad-duct; 3-(2-deoxybeta-dierythropentafuranosyl) pyr [1,2-alpha]-purin-10(3H) one or M1G. This study was undertaken to determine the suitability of M1G as a novel marker of ischemia-reperfusion injury in the liver and its correlation with both the length of Pringle clamp application and the overall length of the operation. METHODS: Normal and colorectal liver metastatic tissues were obtained in 12 patients before and after application of the Pringle manoeuvre. All samples were snap-frozen in liquid nitrogen at -80 ℃. DNA was extracted and M1G quantification was performed by immunoslotblot analysis. RESULTS: M1G levels in normal liver tissue were 4.0 + 1.0 per 107 nucleotides before the Pringle manoeuvre and 7.4 ± 1.0 per 10 nucleotides after the Pringle manoeuvre (mean ± standard deviation) (P<0.05 by ANOVA). M1G levels in malignant liver tissue were 2.5 ±1.4 per 107 nucleotides before the Pringle manoeuvre and 6. 5 ±1.9 adducts per 10 nucleotides after the Pringle manoeuvre (P <0. 05). Ad-duct levels in normal liver tissue showed a significant correlation with cumulative period of Pringle application. CONCLUSIONS: This is the first time that the tissue levels of M1G before and after application of the Pringle manoeuvre have been studied. The results show that the Pringle manoeuvre exerts significant oxidative stress in human hepa-tocytes, which is Pringle-time dependent. The results highlight the potential for oxidative DNA adducts levels as a tool for measuring the severity of ischemia-reperfusion injury. | Giuseppe Garcea Andreas Gescher William Steward Ashley Dennison David Berry | 2006 | Hepatobiliary & Pancreatic Diseases International2006，5，2： | 4 |
| 2 | The diagnosis and management of Sphincter of Oddi dysfunction: a systematic review显示文摘 | Thomas Hall Ashley Dennison Giuseppe Garcea | 2012 | Langenbeck’s Archives of Surgery2012，，6： | 2 |
| 3 | Preoperative biliary drainage for distal biliary obstruction and post‐operative infectious complications显示文摘 | Wee Ngu Michael Jones Chrisopher P. Neal Ashley R. Dennison Matthew S. Metcalfe Giuseppe Garcea | 2012 | ANZ J Surg2012，，4： | 1 |
| 4 | Surgical management of cystic lesions in the liver显示文摘 | Giuseppe Garcea Arumugam Rajesh Ashley R. Dennison | 2013 | ANZ J Surg (-)2013，，7： | 1 |
| 5 | Liver failure after major hepatic resection显示文摘 | Giuseppe Garcea G. J. Maddern | 2009 | Journal of Hepato - Biliary - Pancreatic Surgery2009，，2： | 1 |
| 6 | Outcomes of Pringle maneuver in patients undergoing hepatic resection for colorectal liver metastases显示文摘To the Editor:The Pringle maneuver(PM)was initially described more than a century ago to control bleeding associated with hepatic trauma and it remains the most common method to block hepatic inflow and minimize blood loss during hepatic resections[1]. | Li Lian Kuan Christopher P Neal Vaux Robertson Michael Jones Ashley R Dennison Giuseppe Garcea | 2021 | Hepatobiliary & Pancreatic Diseases International2021，20，6： | 0 |
| 7 | Assessment of long-term graft function following total pancreatectomy and autologous islet transplantation:the Leicester experience显示文摘Background:Total pancreatectomy and islet autotransplantation(TPIAT)is a recognised treatment for chronic pancreatitis(CP)with the potential to mitigate or prevent pancreatogenic diabetes.We present our 10-year follow-up of TPIAT patients.Methods:The University Hospitals of Leicester performed 60 TPIAT procedures from September 1994 to May 2011.Seventeen patients completed their 10-year assessment and were grouped using the modified Auto-Igls criteria;good response,n=5(insulin-independent for first 5 years post-TPIAT);partial response,n=6(insulin requirements<20 iU/day post-TPIAT)and poor response,n=6(insulin requirements≥20 iU/day post-TPIAT).C-peptide,haemoglobin A1c(HbA1c)and oral glucose tolerance test(OGTT)were undertaken preoperatively(baseline),then at 3,6 months and then yearly for 10 years.Data was analysed using analysis of variance(ANOVA).Results:Median C-peptide levels were significantly higher,120 minutes following OGTT,in the“good response”compared to“partial”and“poor”groups(two-way ANOVA test,P<0.0001).All groups demonstrated preservation of C-peptide release.HbA1c levels were significantly lower in the“good response”compared to“partial”and“poor”groups(two-way ANOVA test,P<0.0003 and P<0.0001).Median fasting glucose levels at 30 and 120 min following OGTT,were significantly lower in the“good response”compared to“partial”and“poor”groups(two-way ANOVA test,P<0.0001 and P<0.0001).Conclusions:TPIAT preserves long-term islet graft functions in 10-year follow up.Even in patients in the poor response group,there is evidence of C-peptide release(>0.5 ng/mL)after OGTT stimulation potentially preventing long-term diabetes-related complications. | Cristina APollard Wen Yuan Chung Giuseppe Garcea Ashley R.Dennison | 2023 | Hepatobiliary Surgery and Nutrition2023，12，5： | 0 |
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