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| 1 | Pericyte-like differentiation of human adipose-derived mesenchymal stem cells:An in vitro study显示文摘BACKGROUND Adipose-derived mesenchymal stem cells(ASCs)are characterized by long-term self-renewal and a high proliferation rate.Under adequate conditions,they may differentiate into cells belonging to mesodermal,endodermal or ectodermal lineages.Pericytes support endothelial cells and play an important role in stabilizing the vessel wall at the microcirculation level.The loss of pericytes,as occurs in diabetic retinopathy,results in a breakdown of the blood-retina barrier(BRB)and infiltration of inflammatory cells.In this context,the use of pericytelike differentiated ASCs may represent a valuable therapeutic strategy for restoring BRB damage.AIM To test in vitro strategies to obtain pericyte-like differentiation of human ASCs(hASCs).METHODS Different culture conditions were tested:hASCs cultured in a basal medium supplemented with transforming growth factorβ1;and hASCs cultured in a specific pericyte medium(PM-hASCs).In a further sample,pericyte growth supplement was omitted from the PM.In addition,cultures of human retinal pericytes(hRPCs)were used for comparison.Pericyte-like differentiation of hASCs was tested by immunocytochemical staining and western blotting to evaluate the expression ofα-smooth muscle actin(α-SMA)and neural/glial antigen 2(NG2).Interactions between human retinal endothelial cells(hRECs)and different groups of hASCs were investigated in co-culture experiments.In these cases,the expression of typical junctional proteins such as vascular endothelial-Cadherin,zonula occludens-1 and Occludin were assessed in hRECs.In an in vitro model of the BRB,values of trans-endothelial electrical resistance were measured when hRECs were co-cultured with various groups of pretreated hASCs.The values observed were compared with co-cultures of hRECs and hRPCs as well as with cultures of hRECs alone.Three-dimensional co-cultures of hRECs and hRPCs or pericyte-like hASCs in Matrigel were designed to assess their reciprocal localization.RESULTS After 3-6 d of culture,α-SMA and NG2 immunocytochemistry showed that the closest pericyte-like phenotype was observed when hASCs were cultured in Pericyte Medium(PM-hASCs).In particular,α-SMA immunoreactivity,already visible at the basal level in pericytes and ASCs,was strongly increased only when transforming growth factor was added to the culture medium.NG2 expression,almost undetectable in most conditions,was substantially increased only in PMhASCs.Immunocytochemical results were confirmed by western blot analysis.The presence of pericyte growth supplement seems to increase NG2 expression rather thanα-SMA,in agreement with its role in maintaining pericytes in the proliferative state.In co-culture experiments,immunoreactivity of vascular endothelial-Cadherin,zonula occludens-1 and Occludin was considerably increased in hRECs when hRPCs or PM-hASCs were also present.Supporting results were found by trans-endothelial electrical resistance measurements,gathered at 3 and 6 d of co-culture.The highest resistance values were obtained when hRECs were co-cultured with hRPCs or PM-hASCs.The pericyte-like phenotype of PM-hASCs was also confirmed in three-dimensional co-cultures in Matrigel,where PM-hASCs and hRPCs similarly localized around the tubular formations made by hRECs.CONCLUSION PM-hASCs seem able to strengthen the intercellular junctions between hRECs,likely reinforcing the BRB;thus,hASC-based therapeutic approaches may be developed to restore the integrity of retinal microcirculation. | Giuliana Mannino Florinda Gennuso Giovanni Giurdanella Federica Conti Filippo Drago Salvatore Salomone Debora Lo Furno Claudio Bucolo Rosario Giuffrida | 2020 | World Journal of Stem Cells2020，12，10： | 3 |
| 2 | Potential therapeutic applications of mesenchymal stem cells for the treatment of eye diseases显示文摘Stem cell-based treatments have been extensively explored in the last few decades to develop therapeutic strategies aimed at providing effective alternatives for those human pathologies in which surgical or pharmacological therapies produce limited effects.Among stem cells of different sources,mesenchymal stem cells(MSCs)offer several advantages,such as the absence of ethical concerns,easy harvesting,low immunogenicity and reduced tumorigenesis risks.Other than a multipotent differentiation ability,MSCs can release extracellular vesicles conveying proteins,mRNA and microRNA.Thanks to these properties,new therapeutic approaches have been designed for the treatment of various pathologies,including ocular diseases.In this review,the use of different MSCs and different administration strategies are described for the treatment of diabetic retinopathy,glaucoma,and retinitis pigmentosa.In a large number of investigations,positive results have been obtained by in vitro experiments and by MSC administration in animal models.Most authors agree that beneficial effects are likely related to MSC paracrine activity.Based on these considerations,many clinical trials have already been carried out.Overall,although some adverse effects have been described,promising outcomes are reported.It can be assumed that in the near future,safer and more effective protocols will be developed for more numerous clinical applications to improve the quality of life of patients affected by eye diseases. | Giuliana Mannino Cristina Russo Anna Longo Carmelina Daniela Anfuso Gabriella Lupo Debora Lo Furno Rosario Giuffrida Giovanni Giurdanella | 2021 | World Journal of Stem Cells2021，13，6： | 2 |
| 3 | Management of patients with melanoma and non-melanoma skin cancers in the coronavirus disease 2019 era显示文摘The recent coronavirus disease 2019(COVID-19)pandemic has completely disrupted hospital and outpatient activities.[1,2]It has been reorganized to allow a lower inflow of patients,to reduce non-urgent out-patient visits and to concentrate public health forces in the fight against the COVID-19 outbreak.In this reorganization,dermatology departments have also adapted their activities suspending routine patient visits and providing only urgent surgical removal,urgent visits,consultations,and oncological examinations. | Claudio Conforti Nicola di Meo Roberta Giuffrida Iris Zalaudek | 2020 | Chinese Medical Journal2020，，17： | 2 |
| 4 | Quantification of bioactive acylethanolamides in rat plasma by electrospray mass spectrometry 显示文摘 | Giuffrida A Rodriguez de Fonseca F Piomelli D | 2000 | Anal Biochem2000，280，1： | 1 |
| 5 | Sarcoidosis显示文摘 | Giuffrida TJ Kerdel FA | 2002 | Dermatol Clin2002，20，3： | 1 |
| 6 | Productivity and efficiency changes in primary care: a Malmquist index approach 显示文摘 | Giuffrida A | 1999 | Health Care Management Sci- ence1999，，2： | 1 |
| 7 | Contrala- teral stimulation,using TENS,of phantom limb pain: two con- firmatory cases显示文摘 | GIUFFRIDA O SIMPSON L HALLIGAN P W | 2010 | Pain Med2010，11，1： | 1 |
| 8 | Release of fatty acid amides in a patient with hemispheric stroke: a microdialysis study 显示文摘 | Schabitz W R Giuffrida A Berger C | 2002 | Stroke2002，3，8： | 1 |
| 9 | Acid catalyzed transesterification as a route to poly(3-hydroxybutyrate-co-ε-caprolactone) copolymers from their homopolymers显示文摘 | IMPALLOMENI G GIUFFRIDA M BARBUZZI T | 2002 | Biomacromolecules2002，3，4： | 1 |
| 10 | The recurrent goiter:prevention and management显示文摘 | Cappel ani A Di Vita M Zanghì A Lo Menzo E Caval aro A Alfano G Giuffrida D | | 0，，04： | 1 |
| 11 | The new adipose tissue and adipocytokines显示文摘 | BULCAO C FERREIRA S GIUFFRIDA F | 2006 | Current Diabetes Reviews2006，，2： | 1 |
| 12 | A neural network approach for the differentiation of numerical solutions of 3-D electromagnetic problems 显示文摘 | Capizzi G Coco S Giuffrida C | 2004 | IEEE Transactions on Magnetics2004，40，2： | 1 |
| 13 | Botulinum toxin a,evidence-based exercise therapy,and constraint-induced movement therapy for upper-limb hemiparesis attributable to stroke:a preliminary study显示文摘 | Levy CE Giuffrida C Richards L | 2007 | Am JPhys Med Rehabil2007，86，9： | 1 |
| 14 | An investigation on the use of liquid phase photo-deposition for the preparation of supported Pt catalysts显示文摘 | CRISAFULLI C SCIRE'S GIUFFRIDA S VENTIMIGLIA G LONIGRO R | 2006 | Appl Catal A2006，306，： | 1 |
| 15 | Chondrosarcoma in the United States (1973 to 2003):an analysis of 2890 cases from the SEER database显示文摘 | Giuffrida A Y Burgueno J E Koniaris L G | 2009 | J Bone Joint Surg Am2009，91，5： | 1 |
| 16 | Effects of carbon dioxide pneumoperitoneum, air pneumopeitoneum,and gasless laparoscopy on body weight and tumor growth显示文摘 | Bouvy ND Giuffrida MC Tseng LN | 1998 | Arch Surg1998，133，： | 1 |
| 17 | Memory disturb ances in migraine with and without aura:a strategy prob- lem? 显示文摘 | Le Pira F Zappala G Giuffrida S | 2000 | Cephalaigia2000，20，47： | 1 |
| 18 | Is it worth investigating splenic function in patients with celiac disease?显示文摘Celiac disease, an immune-mediated enteropathy induced in genetically susceptible individuals by the ingestion of gluten, is the most frequent disorder associated with splenic hypofunction or atrophy. Defective splenic function affects more than one-third of adult patients with celiac disease, and it may predispose to a higher risk of infections by encapsulated bacteria and thromboembolic and autoimmune complications, particularly when celiac patients have concomitant pre-malignant and malignant complications (refractory celiac disease, ulcerative jejunoileitis and enteropathy-associated T-cell lymphoma). However, the clinical management of patients with celiac disease does not take into account the evaluation of splenic function, and in patients with high degree of hyposplenism or splenic atrophy the prophylactic immunization with specific vaccines against the polysaccharide antigens of encapsulated bacteria is not currently recommended. We critically re-evaluate clinical and diagnostic aspects of spleen dysfunction in celiac disease, and highlight new perspectives in the prophylactic management of infections in this condition. | Antonio Di Sabatino Laura Brunetti Gabriella Carnevale Maffè Paolo Giuffrida Gino Roberto Corazza | 2013 | World Journal of Gastroenterology2013，19，15： | 1 |
| 19 | Changes in end tidal CO2 and arterial blood gas levels after release of tourniquet显示文摘 | Choi CS Giuffrida JG | 1987 | South Med J1987，80，2： | 1 |
| 20 | A case of ARDS associated with influenza A-H1N1 infection treated with extracorporeal respiratory support显示文摘 | Grasseli G Patroniti N Giuffrida A | 2009 | Minerva Anestesiol2009，75，12： | 1 |
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