| 2 | HLA class Ⅱ alleles and risk for peripheral neuropathy in type 2 diabetes patients显示文摘The potential impact of human leukocyte antigen(HLA) genotype variations on development of diabetic peripheral neuropathy(DPN) is not well determined.This study aimed to identify the association of HLA class II alleles with DPN in type 2 diabetes(T2D) patients.Totally 106 T2 D patients,49 with DPN and 57 without DPN,and 100 ethnic-matched healthy controls were analyzed.Both groups of the patients were matched based on sex,age,body mass index(BMI) and duration of T2 D.Polyneuropathy was diagnosed using electrodiagnostic methods.HLA-DRB1 and DQB1 genotyping was performed in all subjects by the polymerase chain reaction with sequence-specific primers(PCR-SSP) method.T2 D patients with DPN showed higher frequencies of HLA-DRB1*10 and DRB1*12 alleles compared to control group(P = 0.04).HLA-DQB1*O2 allele and HLA-DRB1*O7-DQB1*O2 haplotype were associated with a decreased risk for developing DPN in T2 D patients(P = 0.02 and P = 0.05 respectively).Also,patients with severe neuropathy showed higher frequencies of DRB1*O7(P = 0.003) and DQB1*O2(P = 0.02) alleles than those with mild-to-moderate form of neuropathy.The distribution of DRB1 and DQB1 alleles and haplotypes were not statistically different between all patients and healthy controls.Our findings implicate a possible protective role of HLA-DQB1*O2 allele and HLA-DRB1*O7-DQB1*O2 haplotype against development of peripheral neuropathy in T2 D patients.Therefore,variations in HLA genotypes might be used as genetic markers for prediction and potentially management of neuropathy in T2 D patients. | Ahmad Marzban Javad Kiani Mehrdad Hajilooi Hamzeh Rezaei Zohreh Kahramfar Ghasem Solgi | 2016 | Neural Regeneration Research2016，11，11： | 2 |
| 3 | Promising Effects of Zerumbone on the Regulation of Tumor-promoting Cytokines Induced by TNF-α-activated Fibroblasts显示文摘Inflammation plays an important role in the development of several cancers.Inflammatory cytokines,including tumor necrosis factor-α(TNF-α),are associated with the induction of inflammation.Chronic inflammation contributes to the progression of cancer through several mechanisms,including increased cytokine production and activation of transcription factors,such as nuclear factor-κB(NF-κB).Zerumbone(ZER),a component of subtropical ginger(Zingiber zerumbet Smith),seems to have anti-inflammatory,anti-cancer,and antioxidant activities.In this study,we aimed to explore the protective function and mechanisms of ZER against TNF-α-induced cancer-promoting cytokines.We found that the viability of stimulated human fibroblast cell lines was reduced after treatment with ZER(IC50=18µmol/L),compared to un-stimulated fibroblasts(IC50=40µmol/L).Besides,ZER inhibited mRNA expression and protein secretion of transforming growth factor-β(TGF-β),interleukin-33(IL-33),monocyte chemoattractant protein-1(MCP-1),and stromal cell-derived factor 1(SDF-1),which were produced by TNF-α-induced fibroblasts,as measured by quantitative real time-PCR(qRT-PCR)and ELISA assays.The mRNA expression levels of TGF-β,IL-33,SDF-1,and MCP-1 showed 8,5,2.5,and 4-fold reductions,respectively.Moreover,secretion of TGF-β,IL-33,SDF-1,and MCP-1 was reduced to 3.65±0.34 ng/mL,6.3±0.26,1703.6±295.2,and 5.02±0.18 pg/mL,respectively,compared to the untreated group.In addition,the conditioned media(CM)of TNF-α-stimulated fibroblasts increased the NF-κB expression in colorectal cancer cell lines(HCT-116 and Sw48),while in the vicinity of ZER,the expression of NF-κB was reversed.Considering the significant effects of ZER,this component can be used as an appropriate alternative herbal treatment for cancer-related chronic inflammation. | Zahra Radaei Alireza Zamani Rezvan Najafi Massoud Saidijam Farid Azizi Jalilian Razieh Ezati Ghasem Solgi Razieh Amini | 2020 | Current Medical Science2020，40，6： | 1 |
| 4 | Evaluation of interleukin 8 +2767 A/T polymorphism in visceral leishmaniasis显示文摘Objective:To evaluated the relationship between the genetic variations at IL-8 +2767 position with VL pathogenesis among Iranian patients.Methods:Three groups including patients with VL clinical presentation and leishmania seropositive(n=124).patients seropositive but without clinical presentation(n=82) and healthy controls(n=63) were selected to conduct this cross-sectional study.Polymorphism at +2767 position of IL-8 was investigated using PCR-RPLP techniques.Anti-leishmania antibody titration was evaluated by the immunoflorescence technique.Results:We observed higher significant frequencies +2767 A/A and A/T genotypes in groups I compared to group 2 and healthy controls(P=0.001).Also,patients in Group 1 carriyng A/A genotype showed higher liter of antileshmania antibody than patients with A/T and T/T genotypes(P=0.05).The validity of the data was analyzed using Hardy-Weinberg equilibrium and one way analysis of variance(ANOVA),as well as x^2tests.Conclusions:Our findings indicate that the IL-8 +2767 polymorphism is significantly involved in impaired immune responses against VL and it could be considered as a risk factor for the VL progress. | Alireza Ahmadi Mehrdad Hajilooi Ghasem Solgi Mohammad Abasi Ahad Bazmani Alireza Khalilian Mohammad Matini Khosro Sardarian | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，11： | 0 |
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