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| 1 | Cytokine production in patients with cirrhosis and TLR4 polymorphisms显示文摘AIM:To analyze the cytokine production by peripheral blood cells from cirrhotic patients with and without TLR4 D299G and/or T399I polymorphisms.METHODS:The study included nine patients with cirrhosis and TLR4 D299G and/or T399I polymorphisms,and 10 wild-type patients matched for age,sex and degree of liver failure.TLR4 polymorphisms were determined by sequence-based genotyping.Cytokine production by peripheral blood cells was assessed spontaneously and also after lipopolysaccharide(LPS)and lipoteichoic acid(LTA)stimulation.RESULTS:Patients with TLR4 polymorphisms had a higher incidence of previous hepatic encephalopathy than wild-type patients(78%vs 20%,P=0.02).Spontaneous production of interleukin(IL)-6 and IL-10 was lower in patients with TLR4 polymorphisms than in wild-type patients[IL-6:888.7(172.0-2119.3)pg/m L vs 5540.4(1159.2-26053.9)pg/m L,P<0.001;IL-10:28.7(6.5-177.1)pg/m L vs 117.8(6.5-318.1)pg/m L,P=0.02].However,the production of tumor necrosis factor-α,IL-6 and IL-10 after LPS and LTA stimulation was similar in the two groups.CONCLUSION:TLR4 polymorphisms were associated with a distinctive pattern of cytokine production in cirrhotic patients,suggesting that they play a role in the development of cirrhosis complications. | Juan Camilo Nieto Elisabet Sánchez Eva Román Silvia Vidal Laia Oliva Carlos Guarner-Argente Maria Poca Xavier Torras Cándido Juárez Carlos Guarner German Soriano | 2014 | World Journal of Gastroenterology2014，20，46： | 5 |
| 2 | Effects of cellulase and xylanase enzymes mixed with increasing doses of Salix babylonica extract on in vitro rumen gas production kinetics of a mixture of corn silage with concentrate显示文摘An in vitro gas production(GP) technique was used to investigate the effects of combining different doses of Salix babylonica extract(SB) with exogenous fibrolytic enzymes(EZ) based on xylanase(X) and cellulase(C), or their mixture(XC; 1:1 v/v) on in vitro fermentation characteristics of a total mixed ration of corn silage and concentrate mixture(50:50, w/w) as substrate. Four levels of SB(0, 0.6, 1.2 and 1.8 m L g–1 dry matter(DM)) and four supplemental styles of EZ(1 μL g–1 DM; control(no enzymes), X, C and XC(1:1, v/v) were used in a 4×4 factorial arrangement. In vitro GP(m L g–1 DM) were recorded at 2, 4, 6, 8, 10, 12, 24, 36, 48 and 72 h of incubation. After 72 h, the incubation process was stopped and supernatant p H was determined, and then filtered to determine dry matter degradability(DMD). Fermentation parameters, such as the 24 h gas yield(GY24), in vitro organic matter digestibility(OMD), metabolizable energy(ME), short chain fatty acid concentrations(SCFA), and microbial crude protein production(MCP) were also estimated. Results indicated that there was a SB?EZ interaction(P<0.0001) for the asymptotic gas production(b), the rate of gas production(c), GP from 6 to 72 h, GP2(P=0.0095), and GP4(P=0.02). The SB and different combination of enzymes supplementation influenced(P<0.001) in vitro GP parameters after 12 h of incubation; the highest doses of SB(i.e., 1.8 m L g–1 DM), in the absence of any EZ, quadratically increased(P<0.05) the initial delay before GP begins(L) and GP at different incubation times, with lowering b(quadratic effect, P<0.0001) and c(quadratic effect, P<0.0001; linear effect, P=0.0018). The GP was the lowest(P<0.05) when the highest SB level was combined with cellulose. There were SB?EZ interactions(P<0.001) for OMD, ME, the partitioning factor at 72 h of incubation(PF72), GY24, SCFA, MCP(P=0.0143), and p H(P=0.0008). The OMD, ME, GY24 and SCFA with supp lementation of SB extract at 1.8 m L g–1 DM were higher(P<0.001) than the other treatments, however,PF72 was lower(quadratic effect, P=0.0194) than the other levels. Both C and X had no effect(P>0.05) on OMD, p H, ME, GY24, SCFA and MP. The combination of SB with EZ increased(P<0.001) OMD, ME, SCFA, PF72 and GP24, whereas there was no impact on p H. It could be concluded that addition of SB extract, C, and X effectively improved the in vitro rumen fermentation, and the combination of enzyme with SB extract at the level of 1.2 m L g–1 was more effective than the other treatments. | Abdelfattah Z M Salem German Buendía-Rodríguez Mona M M Elghandour María A Mariezcurrena Berasain Francisco J Pea Jiménez Alberto B Pliego Juan C V Chagoyán María A Cerrillo Miguel A Rodríguez | 2015 | Journal of Integrative Agriculture2015，14，1： | 4 |
| 3 | 酪氨酸羟化酶在C型尼曼-匹克病小鼠小脑中的异常表达显示文摘目的 探讨C型尼曼 匹克病 (NPC)小脑中酪氨酸羟化酶 (TH)异常表达的时间、部位及其与临床表现的关系。 方法 应用TH抗体 ,对不同周龄NPC小鼠和正常小鼠的脑组织切片进行免疫组织化学染色 ,部分相邻切片以抗维生素D依赖性钙结合蛋白抗体染色。 结果 1~ 3周NPC小鼠小脑的浦肯野细胞 (PC)未见明显减少 ,TH染色阴性。 5周时PC开始减少 ,8~ 11周期间PC显著丢失 ,但小脑蚓小结和蚓垂有较多的PC存活。 8周后 ,部分存活的PC及其树突树出现TH免疫反应。其轴突局部球形膨大 ,树突不规则 ,弯曲、断裂、局部膨大 ,树突树萎缩。 结论 NPC1基因突变引起小脑PC严重丢失 ,存活的PC有形态改变和异常基因活动 ,提示其功能不正常 。 | 宋天保 王亚利 梁长林 German D C | 2001 | 解剖学报2001，32，4： | 2 |
| 4 | A Collaborative Study to Develop a Standardized Food Protein Solubility Procedure 显示文摘 | MORt~ C V GERMAN B KINSELLA J E | 1985 | Journal of Food Science1985，50，： | 1 |
| 5 | Geochemistry of a hydrothermal sediment core from the OBS vent- field, 21°N East Pacific rise 显示文摘 | German C R Palmer M Edmond J M | 1999 | Chemical Geology1999，155，： | 1 |
| 6 | Accuracy Assessment and Validation of Classified Satellite Imagery of Texas Coastal Wetlands 显示文摘 | Hinson J M German C D Pulich W J | 1994 | Marine Technology Society Journal1994，，28： | 1 |
| 7 | The Rainbow hydrothermal plume, 36°1S'N, MAR显示文摘 | German C Klinkhammer C P Rudnicki M D | 1996 | Geophysical Re- search Letters1996，23，21： | 1 |
| 8 | First evidence for high-temperature off-axis venting of deep crustal/mantle heat: The Nibelungen hydrothermal field, southern Mid-Atlantic Ridge 显示文摘 | Melchert B Devey C W German C R | 2008 | Earth Planetary Science Letters2008，275，： | 1 |
| 9 | Effect of lubrication mode and compaction temperature on the properties of Fe-Ni-Cu-Mo-C显示文摘 | Degoix C N Griffo A German R M | 1998 | The International Journal of powder Metallurgy1998，34，2： | 1 |
| 10 | Hydrothermal scavenging of rare earthelements in the ocean 显示文摘 | German C R Klinkhammer G P Edmond J M Mura A Elderfield H | 1990 | Nature1990，345，： | 1 |
| 11 | Rituximab in high-grade lymphoma显示文摘 | Zwick C Murawski N Pfreundschuh M German High-Grade Non-Hodgkin Lymphoma Study Group | | 0，，02： | 1 |
| 12 | Effect of lubrication mode and compaction temperature on the properties of Fe-Ni-Cu-Mo-C显示文摘 | GRIF C A GERMAN R M | 1998 | The International Journal of Powder Metallurgy1998，34，2： | 1 |
| 13 | Application of the ceanomaly as a paleoredox indicator:the groud rules显示文摘 | GERMAN C R ELDERFIELD H | 1990 | Paleoceanography1990，5，： | 1 |
| 14 | Hydrothermal activity along the southwest Indian ridge显示文摘 | GERMAN C R BAKER E T MEVEL C et aI | 1998 | Nature1998，395，6701： | 1 |
| 15 | The Rainbow hydrothermal plume, 36° 15' N, MAR 显示文摘 | German C Klinkhammer C P Rudnicki M D | 1996 | Geophysical Research Letters1996，23，21： | 1 |
| 16 | Geochemistry of a hydrothermal sediment core from theOBS vent-fleld, 21 ° N East Pacific Rise 显示文摘 | German C R Hergt J Palmer M R Edmond J M | 1999 | ChemicalGeology1999，155，： | 1 |
| 17 | Bidirectionally Coupled Network and Road Traffic Simulation for Improved IVC Analysis显示文摘 | Sommer C German R Dressler F | 2010 | IEEE Transactions on Mobile Computing2010，10，1： | 1 |
| 18 | Rare earthelement distributions in anoxic waters of the CariacoTrench显示文摘 | DE B H GERMAN C R ELDERFIELD H | 1988 | Geochim Cosmochim Acta1988，52，5： | 1 |
| 19 | Testing community velocity models for southern california using the ambient seismic field显示文摘 | Shuo M German A P Gregory C B | 2008 | Bulletin of the Seismological Society of America2008，98，6： | 1 |
| 20 | Rare earth elements inSaanich Inlet, British Columbia, a seasonally anoxic basin显示文摘 | GERMAN C R ELDERFIELD H | 1989 | Geochim Cosmochim Acta1989，53，10： | 1 |
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