|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Alpha-fetoprotein triggers hepatoma cells escaping from immune surveillance through altering the expression of Fas/FasL and tumor necrosis factor related apoptosis-inducing ligand and its receptor of lymphocytes and liver cancer cells显示文摘AIM: To investigate the mechanism of α-fetoprotein (AFP)in escaping from the host immune surveillance of hepatocellular carcinoma.METHODS: AFP purified from human umbilical blood was administrated into the cultured human lymphoma Jurkat T cell line or hepatoma cell line, Bel7402 in vitro. The expression of tumor necrosis factor related apoptosisinducing ligand (TRAIL) and its receptor (TRAILR) mRNA were analyzed by Northern blot and Western blot wasused to detect the expression of Fas and Fas ligand (FasL)protein.RESULTS: AFP (20 mg/L) could promote the expression of FasL and TRAIL, and inhibit the expression of Fas and TRAILR of Bel7402 cells. For Jurkat cell line, AFP could suppress the expression of FasL and TRAIL, and stimulate the expression of Fas and TRAILR. AFP also could synergize with Bel7402 cells to inhibit the expression of FasL protein and TRAIL mRNA in Jurkat cells. The monoclonal antibody against AFP (anti-AFP) could abolish these functions of AFP.CONCLUSION: AFP is able to promote the expression of FasL and TRAIL in hepatoma cells and enhance the expression of Fas and TRAILR in lymphocytes. These could elicit the escape of hepatocellular carcinoma cells from the host's lymphocytes immune surveillance. | Meng-SenLi Qiu-LingMa QianChen Xin-HuaLiu Ping-FengLi Guo-GuangDu GangLi | 2005 | World Journal of Gastroenterology2005，11，17： | 35 |
| 2 | Alpha-fetoprotein stimulated the expression of some oncogenes in human hepatocellular carcinoma Bel 7402 cells显示文摘AIM:To investigate the molecular mechanism of alphafetoprotein (AFP) on regulating the proliferation of human hepatocellular carcinoma cells.METHODS: Alpha-fetoprotein purified from human umbilical blood was added to cultured human hepatocellular carcinoma Bel 7402 cells in vitro for various treatment periods. The expression of c-fos, c-jun,and N-ras mRNA involved in proliferation and differentiation of cells was analyzed by Northern blot,and the expression of mutative p53 and p21^ras proteins was determined by Western blot.RESULTS:The results showed that AFP (20mg/L) stimulated mRNA expression of these oncogenes in Bel 7402 cells.The expression of c-fos mRNA increased by 51.1%,60.9%,96.0%,and 25.5% at 2, 6, 12, and 24 h, respectively.The expression of c-jun and N-ras mRNA reached to the maximum which increased by 81.3% and 59.9% as compared with the control alter 6h and 24h incubation with AFP, respectively.Western blot assay also demonstrated that AFP promoted the expression of mutative p53 and p21^ras proteins, and the increased rate of those proteins was 13.0%,39.9%, and 70.9%, as well as 35.2%, 102.6%, and 46.8% at 6, 12, and 24h,respectively, as compared with the control.Both human serum albumin (the same dosage as AFP) and monoclonal anti-AFP antibody failed to stimulate the expression of these oncogenes,but anti-AFP antibody could block the functions of AFP.CONCLUSION:The data indicate that AFP can stimulate the expression of some oncogenes to enhance the proliferation of human hepatocellular carcinoma Bel 7402 cells. | Meng-SenLi Ping-FengLi QianChen Guo-GuangDu GangLi | 2004 | World Journal of Gastroenterology2004，10，6： | 19 |
| 3 | Prevalence of hepatitis G virus infection and homology of different viral strains in Southern China显示文摘AIM:To investigate the prevalence of hepatitis G virus(HGV)infection and to andalyse the homology of different HGV strains in Southem Chian. | GangLi Hui-HuiMa GerogeKKLau Yin-KitLeung Chun-LanYao Yu-TianC | 2002 | World Journal of Gastroenterology2002，8，6： | 7 |
| 4 | A new cytokine:the possible effect pathway of methionine enkephalin显示文摘AIM: To investigate experimentally the effects of methionineenkephalin on signal transduction of mouse myeloma NS-1cells.METHODS: The antigen determinate of delta opioidreceptor was designed in this lab and the polypeptidefragment of antigen determinate with 12 amino acidsresidues was synthesized. Monoclonal antibody against thispeptide fragment was prepared. Proliferation of Mouse NS-L cells treated with methionine enkephalin of 1x10-6 mol.L-1was observed. The activities of protein kinase A (PKA) andprotein kinase C (PKC) were measured and thereby themechanism of effect of methionine enkephalin was postulated.RESULTS: The results demonstrated that methionineenkephalin could enhance the proliferation of NS-1 cells andthe effect of methionine enkephalin could be particularlyblocked by monoclonal antibody. The activity of PKA wasincreased in both cytosol and cell membrane. With referenceto PKC, the intracellular activity of PKC in NS-1 cells waselevated at 1x10-7 mol.L-1 and then declined gradually asthe concentration of methionine enkephalin was raised. Theeffects of methionine enkephalin might be reversed by bothnaloxone and monoclonal antibody.CONCLUSION: Coupled with the findings, it in-dicates thatthe signal transduction systems via PKA and PKC are involvedin the effects of methionine enkephalin by binding with thetraditional opioid receptors, and therefore resulting in differentbiological effects. | Xin-HuaLiu Dong-AiHuang Fei-YiYang Yan-ShengHao Guo-GuangDu Ping-FengLi GangLi | 2003 | World Journal of Gastroenterology2003，9，1： | 7 |
| 5 | Discovery of agglutinated foraminifers from the Longzhaogou Group in eastern Heilongjiang Province显示文摘The low diversity agglutinated foraminifers are recovered from the Qihulin Formation of the Longzhaogou Group in eastern Heilongjiang, China. The foraminiferal fauna consists of 9 species of 5 genera. The common members are Cribrostomoides nonioninoides (Reuss), Haplophragmoides concavus (Chapman), H. gigas minor Nauss. Although the diagnostic zonal taxa are absent in the agglutinated fauna, according to the global stratigraphic distribution of the above-mentioned species, and the associated Pseudohaploceras ammonite fauna, the foraminiferal fauna may be of a Barremian-Aptian (Early Cretaceous) age. | LI Gang & YU Shanmao1. State Key Laboratory of Paleobiology Stratigraphy, Nanjing Institute of Geology and Paleontology, Chinese Academy of Sciences, Nanjing 210008, China 2.108th Coal-prospecting Team of Geological Bureau, NE China and Inner Mongolia Coal Company, Jixi 158100, China Correspondence should be addressed to Li Gang (email: gangli@nigpas.ac.cn) | 2004 | Science China Earth Sciences2004，47，2： | 5 |
| 6 | Mechanisms of Apigenin-7-glucoside As a Hepatoprotective Agent显示文摘Ixeris chinesis (Thunb.) Ankai has been used as a Chinese folk medicine, but only scanty information is available on the physiological and biochemical functions of the compounds extracted from I. chinesis. In the present study the effects of apigenin -7-glucoside (APIG) isolated from I. chinesis against liver injury caused by carbon tetrachloride (CCl4) were investigated. Methods The contents of malondialdehyde (MDA), glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT), and reduced glutathione (GSH) were evaluated by spectrophotography. The content of 8-Hydroxydeoxyguanosine (8-OHdG) was measured with high-performance liquid chromatography (HPLC) equipped with electrochemical and UV detection methods. The antioxidant activity of APIG was evaluated using chemiluminescence single photon counting technology. Results CCl4 significantly increased the enzyme activities of GPT and GOT in blood serum, as well as the level of MDA and 8-OHdG in liver tissue, and decreased the levels of GSH. Pretreatment with APIG was able not only to suppress the elevation of GPT, GOT, MDA and 8-OHdG, and inhibit the reduction of GSH in a dose-dependent manner in vivo, but also to reduce the damage of hepatocytes in vitro. On the other hand, we also found that APIG had strong antioxidant activity against reactive oxygen species (ROS) in vitro in a concentration-dependent manner. Conclusion The hepatoprotective activity of APIG is possibly due to its antioxidant properties, acting as scavengers of ROS. These results obtained in vivo and in vitro suggest that APIG has protective effects against hepatic oxidative injury induced by chemicals. Further studies on the pharmaceutical functions and immunological responses of APIG may help its clinical application. | QIU-SHENGZHENG XI-LINGSUN BoXU GANGLI MENGSONG | 2005 | Biomedical and Environmental Sciences2005，18，1： | 4 |
| 7 | Preparation of Pt/C Catalyst with a New and Simple Organic Sol Method显示文摘It is reported for the first time that the Pt/C catalyst can be prepared with a new and simple organic sol method using SnCl2 as the reductant. It was found that the average size of the Pt particles in the Pt/C catalysts could be controlled with controlling the preparation conditions. The effect of the average sizes of the Pt particles in the Pt/C catalysts obtained with this method on the electrocatalytical activity of the oxidation of methanol was investigated. | YaWenTANG GangLI ChangPengLIU WeiX1NG TianHongLU | 2004 | Chinese Chemical Letters2004，15，7： | 4 |
| 8 | Effects of c-myb antisense RNA on TGF-β1 and α1-I collagen expression in cultured hepatic stellate cells显示文摘AIM: To investigate the effects of c-myb antisense RNA on cell proliferation and the expression of c-myb, TGF-β1 and α1-Ⅰ collagen in cultured hepatic stellate cells (HSC) from rats.METHODS: Recombinant retroviral vector of c-myb antisense gene (pDOR-myb) was constructed, and then transfected into retroviral package cell line PA317 by means of DOTAP.The pseudoviruses produced from the resistant PA317 cells were selected with G418 to infect HSCs isolated from rat livers. The cell proliferation was measured by 3-[4,5-Dimethylthiazolzyl]-2, 5-diphenyl tetrazo-dium bromide (MTT) method.The expression of c-myb, α1-Ⅰ collagen and TGF-β1 rnRNA, and c-myb protein in HSCs was detected with semi-quantitive reverse transeription-polymerase chain reaction (RT-PCR) and Western-blot respectively.RESULTS: HSCs from rats were isolated successfully with the viability >98%. In the pDOR-myb infected HSCs, the cmyb protein expression, cell proliferation,and α1-Ⅰ collagen and TGF-β1 mRNA expression were repressed significantly compared with their corresponding control groups (P<0.01).CONCLUSION: c-myb plays a key role in activation and proliferation of HSC. c-myb antisense RNA can inhibit cell proliferation, α1-Ⅰ collagen and TGF-β1 mRNA expression,suggesting that inhibition of c-myb gene expression might be a potential way for the treatment of liver fibrosis. | Hui-HuiMa Ji-LuYao GangLi Chun-LanYao Xue-JuanChen Shao-JiYang | 2004 | World Journal of Gastroenterology2004，10，24： | 3 |
| 9 | CORRECTION FACTORS IN THE DETERMINATION OF MEAN VELOCITY OF OVERLAND FLOW显示文摘 | GANGLI ATHOL D.ABRAHAMS JOSEPH F.ATKINSON | 1998 | Landforms1998，，6： | 2 |
| 10 | Ceria supported Ru^(0)-Ru^(δ+) clusters as efficient catalyst for arenes hydrogenation显示文摘Selective hydrogenation of aromatic amines,especially chemicals such as aniline and bis(4-aminocyclohexyl)methane for non-yellowing polyurethane,is of particular interests due to the extensive applications.To conquer the existing difficulties,in selective hydrogenation,,the Ru^(0)-Ru^(δ+)/CeO2 catalyst with solid frustrated Lewis pairs was developed for aromatic amines hydrogenation with excellent activity and selectivity under relative milder conditions.The morphology,electronic and chemical properties,especially the Ru^(O)-Ru^(δ+) clusters and reducible ceria were characterized by X-ray diffraction(XRD),transmission electron microscopy(TEM),sca nning electronic microscopy(SEM),X-ray photoelectron sp ectroscopy(XPS),CO_(2) tempe rature programmed deso rption(CO_(2)-TPD),H_(2) tempe rature programmed reduction(H_(2)-TPR),H_(2) diffuse reflectance Fourier transform infrared spectroscopy(H_(2)-DRIFT),Raman,etc.The 2% Ru/CeO_(2) catalyst exhibited good conversion of 95% and selectivity greater than 99% toward cyclohexylamine.The volcano curve describing the activity and Ru state was found.Owning to the 'acidic site isolation' by surrounding alkaline sites,condensation between the neighboring amine molecules could be effectively suppressed.The catalyst also showed good stability and applicability for other aromatic amines and heteroarenes containing different functional groups. | Yanwei Cao Huan Zheng Gangli Zhu Haihong Wu Lin He | 2021 | Chinese Chemical Letters2021，32，2： | 2 |
| 11 | Controlled assembly of monolayer-protected gold clusters by dissolved DNA显示文摘 | WANG Gangli MURRAY R W | 2004 | Nano Lett2004，4，1： | 1 |
| 12 | Study on Stability of Nateglinide Polymorphism显示文摘The stability of three forms of nateglinide, especially S-form and H-form, was determined. S-form was a new crystal structure1 of nateglinide. Three forms of nateglinide were treated in different conditions, such as in various temperatures, humidity, light and so on. Analysis of their crystal structures was performed by X-ray powder diffraction ( XRD ) and their particle shapes were observed with scanning electron microscope ( SEM ). The results indicated that the stability of S-form of nateglinide is the best among the three forms and their particle shapes are quite difference. S-form is the sheet structure of layer upon layer, H-form looks like a hank of silk lines and B-form is the clubbed shape. | GuoQiangSU GangLI QunWeiXU XiangYinMO JiaYingCHEN | 2003 | Chinese Chemical Letters2003，14，7： | 1 |
| 13 | For the Bright Future—Bulk Heterojunction Polymer Solar Cells with Power Conversion Efficiency of 7.4%显示文摘 | YongyeLiang ZhengXu JiangbinXia Szu‐TingTsai YueWu GangLi ClaireRay LupingYu | 2010 | Adv Mater2010，，20： | 1 |
| 14 | For the Bright Future—Bulk Heterojunction Polymer Solar Cells with Power Conversion Efficiency of 7.4%显示文摘 | YongyeLiang ZhengXu JiangbinXia Szu‐TingTsai YueWu GangLi ClaireRay LupingYu | 2010 | Adv. Mater2010，，20： | 1 |
| 15 | Synthesis and in vitro activity of the penem anfibiotics显示文摘 | Mc Combie SW Gangly AK | 1998 | Med Res Rev1998，8，3： | 1 |
| 16 | Effects of valsartan on ventricular arrhythmia induced by programmed electrical stimulation in rats with myocardial infarction显示文摘 | Kun‐LiJiao Yi‐GangLi Peng‐PaiZhang Ren‐HuaChen YiYu | 2012 | Journal of Cellular and Molecular Medicine2012，，： | 1 |
| 17 | Biodiesel produc- tion with water-tolerance and microwave absorbing cata- lyst using tung oil 显示文摘 | Yuan Hong Yang Bolun Zhu Gangli | 2013 | International journal of green ener- gy2013，10，10： | 1 |
| 18 | Bio-oxidation as a viable method for SOx emission control显示文摘 | P Gangli | 1998 | The Canadian Mining and Metallurgical Bulletin1998，91，1016： | 1 |
| 19 | For the Bright Future—Bulk Heterojunction Polymer Solar Cells with Power Conversion Efficiency of 7.4%显示文摘 | YongyeLiang ZhengXu JiangbinXia Szu‐TingTsai YueWu GangLi ClaireRay LupingYu | 2010 | Mater2010，，20： | 1 |
| 20 | Gene mutation analysis and immune checkpoint therapy in head and neck squamous cell carcinoma显示文摘Immune checkpoint inhibitors(ICI),represented by blocked programmed cell death-1(PD-1),is a group of novel medicines for anti-tumor immunotherapy.It has been approved by the U.S.Food and Drug Administration(FDA)in recent years for relapsed or metastatic head and neck squamous cell carcinoma(HNSCC),and brings promising treatment prospects.However,the instability caused by tumor gene mutations significantly compromises the therapeutic effect of ICI.Therefore,the identification and analysis of HNSCC gene mutations can further guide and optimize the application of ICIs in HNSCC.In this study,we preliminarily described the clinical research progress of ICI therapy and the potential immune escape mechanism in HNSCC.An overview of complete HNSCC gene mutation results was generated from the bioinformatics study of TCGA database to further explain and analyze the relevant molecular mechanisms,which may aid in designing future personalized therapeutic strategies for HNSCC patients. | Hua Yang Yuxue Wei Gangli Liu | 2022 | Oncology and Translational Medicine2022，8，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员