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| 1 | Protective Effect of Magnesium Isoglycyrrhizinate on Ethanol-Induced Testicular Injuries in Mice显示文摘Objective:Ethanol treatment induces an increase in oxidative stress. As licorice compounds are potent antioxidants, our aim was to examine whether magnesium isoglycyrrhizinate attenuated lipid peroxidation, the major end-point of oxidative damage resulting from ethanol administration. Methods: Four groups(18 animals in each group) of male Kunming mice were used. The first group served as control and received 0.4 ml normal saline daily for 18 days orally. The second group of mice was given 56% ethanol at 16 ml/kg body weight per day for 18 days orally. The third group was given the same dose of ethanol and administrated magnesium isoglycyrrhizinate (15 mg/kg.d, i.p.) for 18 days. The fourth group was given the same dose of ethanol and administrated with magnesium isoglycyrrhizinate (45 mg/kg.d, i.p.) for 18 days. Twenty four hours after 9 days or 18 days of treatment the mice were sacrificed using 10% chloral hydrate. Sperm counts and motility in the epididymis were assessed. The lipid peroxidation and antioxidants of testicular mitochondria were also determined. The pathological changes of testicle tissue of the mice were observed by light microscopy. Results: Magnesium isoglycyrrhizinate effectively prevented the ethanol-induced seminiferous epithelium disorganization and degeneration of Sertoli cells and germ cells. Sperm counts and motility of the magnesium isoglycyrrhizinate treated groups were higher than those of the alcohol treated group, but were lower than those of the control group. The drug exhibited an ability to counteract ethanol induced oxidative challenge as it effectively reduced testicular malondialdehyde (MDA) and increased the activities of superoxide dismutase and glutathione peroxidase. Conclusion: Magnesium isoglycyrrhizinate is able to inhibit the ethanol-induced lipid peroxidation and has a protective effect against testicular oxidative injury. | Yuanqiao He Fuqing Zeng Qing Liu Wen Ju Houju Fu Hua Hao Lulu Lia Yifeng Xie | 2010 | The Journal of Biomedical Research2010，24，2： | 9 |
| 2 | Fabric changes induced by super-absorbent polymer on cementelime stabilized excavated clayey soil显示文摘This paper studies the microstructure variation induced by super-absorbent polymer(SAP)to understand the mechanism of macroscopic strength improvement of stabilized soil.The fabric changes of cement elime stabilized soil were analyzed with respect to the variation of SAP content,water content,lime content and curing time,using mercury intrusion porosimetry(MIP)tests.It can be observed that the delimitation pore diameter between inter-and intra-aggregate pores was 0.2 mm for the studied soil,determined through the intrusion/extrusion cycles.Experimental results showed that fabric in both inter-and intra-aggregate pores varied significantly with SAP content,lime content,water content and curing time.Two main changes in fabric due to SAP are identified as:(1)an increase in intra-aggregate pores(<0.2 mm)due to the closer soilecementelime cluster space at higher SAP content;and(2)a decrease in inter-aggregate pores represented by a reduction in small-pores(0.2e2 mm)due to the lower pore volume of soil mixture after water absorption by SAP,and a slight increase in large-pores(>2 mm)due to the shrinkage of SAP particle during the freezeedry process of MIP test.Accordingly,the strength gain due to SAP for cementelime stabilized soil was mainly due to a denser fabric with less interaggregate pores.The cementitious products gradually developed over time,leading to an increase in intra-aggregate pores with an increasing proportion of micro-pores(0.006e0.2 mm).Meanwhile,the inter-aggregate pores were filled by cementitious products,resulting in a decrease in total void ratio.Hence,the strength development over time is attributable to the enhancement of cementation bonding and the refinement of fabric due to the increasing cementitious compounds. | Xia Bian Lingling Zeng Xiaozhao Li Xiusong Shi Shuming Zhou Fuqing Li | 2021 | Journal of Rock Mechanics and Geotechnical Engineering2021，13，5： | 3 |
| 3 | The inhibitory effects of siRNA expression vector on CXCR4 expression in prostate carcinoma cell lines显示文摘Objective: To investigate the inhibitory effects of RNAi (RNA interference, RNAi) expression vector on CXCR4 expression in prostate carcinoma cell lines. Methods: Small interference RNA(siRNA) expression vectors for CXCR4 gene were constructed and transfected into prostate carcinoma cell lines(PC-3m and LNCaP)with liposomes. The expression of CXCR4 was detected by RT-PCR and western blot. Results: The expression of CXCR4 mRNA and protein in the PC-3m and LNCaP cells was reduced by RNAi expression vectors. The inhibitory rate of CXCR4 mRNA expression in the PC-3m cells was 87.81%±10.20%,56.10%±9.32% at the 24th hour and the 48th hour, compared with 56.93%±8.78%,49.24%±11.23% in LNCaP cells. The inhibitory rate of the expression of CXCR4 protein was 64.71%±6.68%,58.66%±11.56% respectively. Conclusion: The expression of CXCR4 gene can effectively be inhibited by RNAi expression vectors. | Yuefeng Du Yifei Xing Fuqing Zeng Peng Lu Xianyin Liu | 2006 | Journal of Nanjing Medical University2006，20，2： | 3 |
| 4 | Methyl jasmonate sensitizes human bladder cancer cells to gambogic acid‐induced apoptosis through down‐regulation of EZH 2 expression by miR ‐101显示文摘 | Yongjun Wang Wei Xiang Miao Wang Tao Huang Xingyuan Xiao Liang Wang Dan Tao Liyun Dong Fuqing Zeng Guosong Jiang | 2014 | Br J Pharmacol2014，，3： | 2 |
| 5 | Detection of Smac expression in bladder cancer and its clinical significance显示文摘Objective: To detect the expression of second mitochondria-derived activator of caspase (Smac) in bladder cancer and discuss its clinical significance. Methods: Smac was detected in 15 specimens of normal bladder epithelium and 72 specimens of bladder cancer by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry at the level of gene and protein, respectively. Results: The differences of both Smac protein and mRNA expressions between normal mucous membrane of bladder and grade Ⅰ bladder cancer had no statistical significance(P>0.05). The expressions of Smac protein and its mRNA in bladder cancer decreased gradually with the advance of bladder cancer (P<0.01 and P<0.05, respectively). In invasive bladder cancer, the expressions of Smac protein and its mRNA were higher than those in superficial bladder cancer (P<0.01). Conclusions: Normal bladder epithelium has high expression of Smac while bladder cancer has low expression of Smac. The expression of Smac is closely related to the grade and stage of bladder cancer. Detection of Smac expression helps to judge the grade and stage of bladder cancer and Smac gene might become a valid target for gene therapy of bladder cancer. | Guiyi Liao Fuqing Zeng Xianghui Yue Liang Wang Fangmin Cheng | 2006 | Journal of Nanjing Medical University2006，20，1： | 1 |
| 6 | Expression and significance of Oct4 in bladder cancer显示文摘 | Kai Xu Zhaohui Zhu Fuqing Zeng Jihua Dong | 2008 | Journal of Huazhong University of Science and Technology2008，，6： | 1 |
| 7 | Construction of Smac gene-containing and human prostate specific antigen promoter-regulated vector and its expression显示文摘Objective: To construct an eukaryotic expression vector containing Smac gene and study the expression efficiency and speci- ficity of prostate specific antigen(PSA) enhancer/promoter in a possible targeted gene therapy scheme for prostate cancer. Meth- ods: PSA enhancer (PSAE) and promoter (PSAP) sequences were amplified using PCR method. CMV and T7 promoters were deleted from pcDNA3.1-Smac and replaced by the two specific fragments to generate pPSAE-PSAP-Smac. After transfection into different cell lines, the status of cells was observed. And then, we determined the relative concentration of Smac mRNA in RT-PCR. Results: The recombinant plasmid of pPSAE-PSAP-Smac was successfully constructed. And only the prostate cancer cell line PC-3 was suppressed after transfection with pPSAE-PSAP-Smac. However, other nonprostate lines were not. Moreover, the concentration of Smac mRNA regulated by PSA promoter and enhancer was higher in comparison to the CMV promoter-driven control vectors. Conclusion: An expression vector containing the Smac gene (based on elements of the PSA gene regulatory se- quences) has been developed and shown to function in prostate cancer cell lines which provides a solid platform for launching clin- ical studies. | Yu Wu Fuqing Zeng Liang Wang Yanbo Wang Guiyi Liao | 2007 | Journal of Nanjing Medical University2007，21，3： | 1 |
| 8 | Methyl jasmonate sensitizes human bladder cancer cells to gambogic acid‐induced apoptosis through down‐regulation of EZH 2 expression by miR ‐101显示文摘 | Yongjun Wang Wei Xiang Miao Wang Tao Huang Xingyuan Xiao Liang Wang Dan Tao Liyun Dong Fuqing Zeng Guosong Jiang | | Br J Pharmacol0，，3： | 1 |
| 9 | Preparation of arsenic trioxide albumim microspheres and its release characteristics in vitro显示文摘 | Zhou Jie Zeng Fuqing Gao Xiang Xie Shusheng Wei Shuli | 2005 | Journal of Huazhong University of Science and Technology [Medical Sciences]2005，，3： | 1 |
| 10 | Construction of Smac gene-carrying and human uroplakin Ib promoter-Regulated Genetic Vector and its Expression显示文摘Objective: To construct an eukaryotic expression vector that contains Smac gene, which is regulated by human Uroplakin Ib (UpIb) promoter. Methods: For the directionality of Smac expression in the transitional cell carcinoma of bladder, internal CMV and T7 promoter sequences in eukaryotic expression vector pcDNA3.1-Smac were replaced with UpIb promoter to construct a new plasmid. The plasmid DNA was identified by gel electrophoresis after being double digested at respective sites, and then the sequence was analyzed. The expression of Smac mRNA and protein in BIU87 cell line were detected after the transfection by using the newly constructed vector. Results: The Smac gene-carrying and UpIb promoter-regulated eukaryotic expression vector pcDNA3-UpIb-promoter-Smac was successfully constructed. The expression of Smac mRNA was approximately increased by 2.1 times and the expression of Smac protein was increased in about 71% BIU87 cells. Conclusion: The new vector can be effectively expressed in bladder cancer cells and be of great significance for bladder cancer-targeted gene therapy. | Zhongxing Zhang Fuqing Zeng Guiyi Liao Xianghui Yue | 2006 | Journal of Nanjing Medical University2006，20，5： | 0 |
| 11 | Combination of CTLA4-FasL gene transfer and allogeneic bone marrow transplantation led to durable macrochimerism and donor-specific tolerance in mouse model显示文摘Mixed hemopoietic chimerism is capable of inducing donor specific tolerance, thus eliminating the chronic immunosuppressive therapy following organ trans- plantation. As yet no safe and effective tolerance protocol is available for clinical implementation. Here we describe an alternative nonmyeloablative based strategy of using a single injection of recombination adenovirus vector encoding CTLA4-FasL fusing gene and donor bone marrow cells to promote durable mixed macrochimerism (>20% on 140 d). Chimeras exhibited robust donor-specific tolerance, as evi- denced by acceptance of fully allogeneic skin grafts (the mean survival time (MST)>200 d) and rejection of third- party skin grafts in a normal manner (MST<10 d). In this model, the frequencies of helper T lymphocyte precursor (HTLp) and cytotoxic T lymphocyte precursor (CTLp) were greatly reduced on day 14 after transplantation, suggesting that CTLA4-FasL led to rapid systemic peripheral tolerance to facilitate the bone marrow engraftment, while both HTLp and CTLp remained at low level only in recipient mice with mixed chimerism on day 140 after transplantation, demon- strating that long-term skin grafts tolerance was associated with stable mixed chimerism, and central deletion of donor specific T cell may be the main mechanism for tolerance maintenance. | FENG Yougang WANG Guangming HAO Jie LI Ailing YUAN Guohong LI Chong ZENG Fuqing XlE Shusheng | 2005 | Chinese Science Bulletin2005，50，20： | 0 |
| 12 | Effect on proliferation and apoptosis of T24 cell lines via silencing DNMT1 with RNA interference显示文摘Expression of DNA methyltransferase 1(DNMT1),which plays an important role on aberrantly methylated CpG in the promoter regions of tumor sup-pressor genes(TSGs),is higher in bladder cancer cells than in normal bladder cells.Therefore,its overexpression is closely related to tumor formation.In this study,the eukaryotic vector pshRNA-DNMT1 was constructed and transfected into T24 cells.Levels of DNMT1 mRNA and protein were detected by reverse transcription-polymerase chain reaction(RT-PCR)and western blot.Relative to the blank control at the 24th,48th and 72nd hour after transfection of pshRNA-DNMT1,the inhibitory rates of DNMT1 mRNA levels in T24 cells were 28.44%,52.48%,70.91%,respectively.Those of DNMT1 proteins were 24.27%,57.79%,and 77.74%,respectively.Proliferation and apoptosis were assayed by MTT and flow cytometry with Annexin-V-FITC/PI staining.The growth inhibition rates of pshRNA-DNMT1 at the 24th,48th and 72nd hour after transfection of pshRNA-DNMT1 were(4.34¡0.76)%,(9.87¡1.54)%and(13.78¡1.93)%,respectively.There were statistically significant differ-ences between pshRNA-DNMT1 and the control blank at each time points(P,0.01);24,48 and 72 hours after T24 cells were transfected by pshRNA-DNMT1,the apoptosis rates of pshRNA-DNMT1 were(3.87¡0.81)%,(8.69¡1.23)%and(11.46¡1.24)%,respectively(P,0.01 vs blank control).Based on this case,our conclu-sion is that the recombinant plasmid pshRNA-DNMT1 can silence the expression of gene DNMT1 mRNA and protein effectively,and to some extent,it also can inhibit the proliferation of bladder cancer cell and promote the cellular apoptosis. | Shilong ZHANG Fuqing ZENG Shibo PENG Liang WANG | 2008 | Frontiers of Medicine2008，2，4： | 0 |
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