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| 1 | Diabetes inhibits corneal epithelial cell migration and tight junction formation in mice and human via increasing ROS and impairing Akt signaling显示文摘Corneal wounds usually heal quickly;but diabetic patients have more fragile corneas and experience delayed and painful healing. In the present study, we compared the healing capacity of corneal epithelial cells (CECs) between normal and diabetic conditions and the potential mechanisms. Primary murine CEC derived from wild-type and diabetic (db/db) mice, as well as primary human CEC were prepared. Human CEC were exposed to high glucose (30 mM) to mimic diabetic conditions. Cell migration and proliferation were assessed using Scratch test and MTT assays, respectively. Reactive oxygen species (ROS) production in the cells was measured using dichlorofluorescein reagent. Western blot was used to evaluate the expression levels of Akt. Transepithelial electrical resistance (TEER) and zonula occludens-1 (ZO-1) expression were used to determine tight junction integrity. We found that the diabetic CEC displayed significantly slower cell proliferation and migration compared with the normal CEC from both mice and humans. Furthermore, ROS production was markedly increased in CEC grown under diabetic conditions. Treatment with an antioxidant N-acetyl cysteine (NAC, 100 μM) significantly decreased ROS production and increased wound healing in diabetic CEC. Barrier function was significantly reduced in both diabetic mouse and human CEC, while NAC treatment mitigated these effects. We further showed that Akt signaling was impaired in diabetic CEC, which was partially improved by NAC treatment. These results show that diabetic conditions lead to delayed wound-healing capacity of CEC and impaired tight junction formation in both mice and human. Increased ROS production and inhibited Akt signaling may contribute to this outcome, implicating these as potential targets for treating corneal wounds in diabetic patients. | Qi-wei Jiang Denis Kaili Jonaye Freeman Chong-yang Lei Bing-chuan Geng Tao Tan Jian-feng He Zhi Shi Jian-jie Ma Yan-hong Luo Heather Chandler Hua Zhu | 2019 | Acta Pharmacologica Sinica2019，40，9： | 8 |
| 2 | The measurement of migration of the acetabular component of hip prostheses显示文摘 | Nunn D Freeman MA Hill PF | 1989 | J Bone Joint Surg Br1989，71，： | 1 |
| 3 | Maintenance intravenous fluid prescribing practices among paediatric residents显示文摘 | Freeman MA Ayus JC Moritz ML | 2012 | Acta Paediatrica2012，101，10： | 1 |
| 4 | Acute gangrenous cholecystitis di-agnosed on gallium scan显示文摘 | MA HY FREEMAN LM | 2014 | Clin Nucl Med2014，39，3： | 1 |
| 5 | The patellofemoral joint in total knee arthroplasty:is the design of the trochlea the critical factor显示文摘 | Kulkarni SK Freeman MA Poal-Manresa JC | | 0，，： | 1 |
| 6 | Mechanical initiation of intervertebral discdegeneration 显示文摘 | Adams MA Freeman B J Morrison HP | 2000 | Spine ( Phila Pa 1976 )2000，25，： | 1 |
| 7 | A quantitative study using a tissue-digestion method显示文摘 | Kobayashi A Freeman MA Bonfield W Number of polyethylene particles and osteolysis in total joint replacements | 1997 | J Bone Joint Surg Br1997，79，5： | 1 |
| 8 | Body mass index refer- ence curves for the UK, 1990显示文摘 | COLE TJ FREEMAN JV PREECE MA | 1995 | Arch Dis Child1995，73，1： | 1 |
| 9 | The patellofemoral joint in total knee arthroplasty:is the design of the trochlea the critical factor?显示文摘 | Kulkarni SK Freeman MA Poal-Manresa JC | 2000 | J Arthroplasty2000，15，4： | 1 |
| 10 | Instability of the foot after injuries to the lateral ligment of the ankle显示文摘 | Freeman MA | 1965 | J Bone Joint Surg Br1965，47，4： | 1 |
| 11 | The role of the lumbar multifidus in chronic low back pain: a review 显示文摘 | Freeman MD Woodham MA Woodham AW | 2010 | PM R2010，2，14： | 1 |
| 12 | Mechanical initiation of intervertebral disc degeneration显示文摘 | Adams MA Freeman BJ Morrison HP | 2000 | Spine2000，25，13： | 1 |
| 13 | Motion device:An alternative to physical restraints显示文摘 | Freeman MA | 2004 | Geriatr Nurs2004，25，3： | 1 |
| 14 | Fellow eyes of eyes with macular holes显示文摘 | Bronstein MA Trempe CL Freeman HM | 1981 | Am J Ophthalmol1981，92，6： | 1 |
| 15 | Intramolecular carbon isotopic analysis of aceticacid by direct injection of aqueous solution显示文摘 | THOM AS B FREEMAN K H ARTHUR MA | 2009 | Organic Geochemistry2009，40，2： | 1 |
| 16 | Engineering considerations in the internal fixation of fractures of the tibial shaft显示文摘 | Laurence M Freeman MA Swanson SA | 1969 | J Bone Joint Surg Br1969，51，4： | 1 |
| 17 | Body mass index ref- erence curves for the UK, 1990显示文摘 | COLE TJ FREEMAN JV PREECE MA | 1995 | Arch Dis Child1995，73，1： | 1 |
| 18 | Atrial arrhythmia after surgical closure of atrial septal defects in adults显示文摘 | Gatzoulis MA Freeman MA Siu SM | 1999 | N Engl J Med1999，340，： | 1 |
| 19 | Allelic delection analysis of the FHIT gene predicts poor survival in non-small cell lung cancer显示文摘 | Burke L Khan MA Freeman AN | 1998 | Cancer Res1998，58，12： | 1 |
| 20 | HORIZON: An open-label extension trial of ranibizumab for choroidal neovascularization secondary to age-related macu- lar dengeration 显示文摘 | Singer MA Awh CC Sadda S Freeman WR Antoszyk AN Wong P | 2012 | Ophthalmology2012，119，6： | 1 |
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