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| 1 | Induced pluripotent stem cells for modeling neurological disorders显示文摘Several diseases have been successfully modeled since the development of induced pluripotent stem cell(i PSC) technology in 2006. Since then, methods for increased reprogramming efficiency and cell culture maintenance have been optimized and many protocols for differentiating stem cell lines have been successfully developed, allowing the generation of several cellular subtypes in vitro. Gene editing technologies have also greatly advanced lately, enhancing disease-specific phenotypes by creating isogenic cell lines, allowing mutations to be corrected in affected samples or inserted in control lines. Neurological disorders have benefited the most from i PSC-disease modeling for its capability for generating disease-relevant cell types in vitro from the central nervous system, such as neurons and glial cells, otherwise only available from post-mortem samples. Patient-specific i PSC-derived neural cells can recapitulate the phenotypes of these diseases and therefore, considerably enrich our understanding of pathogenesis, disease mechanism and facilitate the development of drug screening platforms for novel therapeutic targets. Here, we review the accomplishments and the current progress in human neurological disorders by using i PSC modeling for Alzheimer's disease, Parkinson's disease, Huntington's disease, spinal muscular atrophy, amyotrophic lateral sclerosis, duchenne muscular dystrophy, schizophrenia and autism spectrum disorders, which include Timothy syndrome, Fragile X syndrome, Angelman syndrome, Prader-Willi syndrome, PhelanMc Dermid, Rett syndrome as well as Nonsyndromic Autism. | Fabiele B Russo Fernanda R Cugola Isabella R Fernandes Graciela C Pignatari Patricia C B Beltrão-Braga | 2015 | World Journal of Transplantation2015，5，4： | 4 |
| 2 | Value of carcinoembryonic antigen and cytokeratins for the detection of recurrent disease following curative resection of colorectal cancer显示文摘瞄准:与颜色在病人为周期性的疾病的察觉评估 carcinoembryonic 抗原(CEA ) 和 cytokeratins 的手术后的连续试金的功效在激进的外科以后的表面的腺癌。方法:在 1993 和 2000 之间,有颜色的 120 个病人表面的腺癌在外科的肠胃病学的部门经历了激进的外科, Sao Paulo-Escola Paulista de Medicina 的联邦大学, Sao Paulo,巴西。周期的手术后的评估被 assaying 标记在外部浆液,结肠镜检查和成像检查执行。CEA 的存在与 72 U/L 阀值用 LIA 地席 TPA-M Prolifigen 方法与 5 microg/L 阀值,和 cytokeratins 用 Delfia 方法被检测。结果:在第一手术后的年里,没有周期性的疾病的病人有 CEA 的正常层次( 1.5 +/- 0.9 microg/L )并且单音的同种细胞的织物多肽 antigen-M ( TPA-M , 64.4 +/- 47.8 U/L ),当有复发的病人有 CEA 的高水平时( 6.9 +/- 9.8 microg/L , P <
0.01 )并且 TPA-M ( 192.2 +/- 328.8 U/L , P <
0.05 )。在第二手术后的年期间,没有肿瘤复发的病人有 CEA 的正常层次( 2.0 +/- 1.8 microg/L )并且 TPA-M ( 50.8 +/- 38.4 U/L ),当有复发的病人有 CEA 的高水平时( 66.3 +/- 130.8 microg/L , P <
0.01 )并且 TPA-M ( 442.7 +/- 652.8 U/L , P <
0.05 )。吝啬的后续时间是 22.3 瞬间。在 23 种情况中有复发。没有完成激进的切除,五个手术被动。在肿瘤标记层次的上升先于复发的鉴定:在七的 CEA (30%) 和在十一个个人(48%) 的 TPA-M。结论:由 CEA 和 cytokeratins 的连续试金的集中的后续允许颜色的早察觉表面的瘤复发。 | Luís C Fernandes Su B Kim Sarhan S Saad Delcio Matos | 2006 | World Journal of Gastroenterology2006，12，24： | 3 |
| 3 | Quality of life, work productivity impairment and healthcare resources in inflammatory bowel diseases in Brazil显示文摘BACKGROUND Inflammatory bowel diseases(IBD)have been associated with a low quality of life(QoL)and a negative impact on work productivity compared to the general population.Information about disease control,patient-reported outcomes(PROs),treatment patterns and use of healthcare resources is relevant to optimizing IBD management.AIM To describe QoL and work productivity and activity impairment(WPAI),treatment patterns and use of healthcare resources among IBD patients in Brazil.METHODS A multicenter cross-sectional study included adult outpatients who were previously diagnosed with moderate to severe Crohn’s disease(CD)or ulcerative colitis(UC).At enrolment,active CD and UC were defined as having a Harvey Bradshaw Index≥8 or a CD Activity Index≥220 or calprotectin>200μg/g or previous colonoscopy results suggestive of inadequate control(per investigator criteria)and a 9-point partial Mayo score≥5,respectively.The PRO assessment included the QoL questionnaires SF-36 and EQ-5D-5L,the Inflammatory Bowel Disease Questionnaire(IBDQ),and the WPAI questionnaire.Information about healthcare resources and treatment during the previous 3 years was collected from medical records.Chi-square,Fisher’s exact and Student’s t-/Mann-Whitney U tests were used to compare PROs,treatment patterns and the use of healthcare resources by disease activity(α=0.05).RESULTS Of the 407 patients in this study(CD/UC:64.9%/35.1%,mean age 42.9/45.9 years,54.2%/56.6%female,38.3%/37.1%employed),44.7%/25.2%presented moderate-to-severe CD/UC activity,respectively,at baseline.Expressed in median values for CD/UC,respectively,the SF-36 physical component was 46.6/44.7 and the mental component was 45.2/44.2,the EQ-visual analog scale score was 80.0/70.0,and the IBDQ overall score was 164.0/165.0.Moderate to severe activity,female gender,being unemployed,a lower educational level and lower income were associated with lower QoL(P<0.05).Median work productivity impairment was 20%and 5%for CD and UC patients,respectively,and activity impairment was 30%,the latter being higher among patients with moderate to severe disease activity compared to patients with mild or no disease activity(75.0%vs 10.0%,P<0.001).For CD/UC patients,respectively,25.4%/2.8%had at least one surgery,38.3%/19.6%were hospitalized,and 70.7%/77.6%changed IBD treatment at least once during the last 3 years.The most common treatments at baseline were biologics(75.3%)and immunosuppressants(70.9%)for CD patients and 5-ASA compounds(77.5%)for UC patients.CONCLUSION Moderate to severe IBD activity,especially among CD patients,is associated with a substantial impact on QoL,work productivity impairment and an increased number of IBD surgeries and hospitalizations in Brazil. | Rogerio Serafim Parra Julio MF Chebli Heda MBS Amarante Cristina Flores Jose ML Parente Odery Ramos Milene Fernandes Jose JR Rocha Marley R Feitosa Omar Feres Antonio S Scotton Rodrigo B Nones Murilo M Lima Cyrla Zaltman Carolina D Goncalves Isabella M Guimaraes Genoile O Santana Ligia Y Sassaki Rogerio S Hossne Mauro Bafutto Roberto LK Junior Mikaell AG Faria Sender J Miszputen Tarcia NF Gomes Wilson R Catapani Anderson A Faria Stella CS Souza Rosana F Caratin Juliana T Senra Maria LA Ferrari | 2019 | World Journal of Gastroenterology2019，25，38： | 2 |
| 4 | Can baseline ML Flow test results predict leprosy reactions? An investigation in a cohort of patients enrolled in the uniform multidrug therapy clinical trial for leprosy patients in Brazil显示文摘Background:The predictive value of the serology to detection of IgM against the Mycobacterium leprae-derived phenolic glycolipid-I/PGL-I to identify leprosy patients who are at higher risk of developing reactions remains controversial.Whether baseline results of the ML Flow test can predict leprosy reactions was investigated among a cohort of patients enrolled in The Clinical Trial for Uniform Multidrug Therapy for Leprosy Patients in Brazil(U-MDT/CT-BR).Methods:This was a descriptive study focusing on the main clinical manifestations of leprosy patients enrolled in the U-MDT/CT-BR from March 2007 to February 2012 at two Brazilian leprosy reference centers.For research purposes,753 leprosy patients were categorized according to a modified Ridley-Jopling(R&J)classification and according to the development of leprosy reactions(reversal reaction/RR and erythema nodosum leprosum/ENL),and whether they had a positive or negative bacillary index/BI.Results:More than half of the patients(55.5%)reported leprosy reaction:18.3%(138/753)had a RR and 5.4%(41/753)had ENL.Leprosy reactions were more frequent in the first year following diagnosis,as seen in 27%(205/753)of patients,while 19%(142/753)developed reactions during subsequent follow-up.Similar frequencies of leprosy reactions and other clinical manifestations were observed in paucibacillary(PB)and multibacillary(MB)leprosy patients treated with U-MDT and regular MDT(R-MDT)(P=0.43 and P=0.61,respectively).Compared with PB patients,leprosy reactions were significantly more frequent in MB patients with a high BI,and more patients developed RR than ENL.However,RR and neuritis were also reported in patients with a negative BI.At baseline,the highest rate of ML Flow positivity was observed in patients with a positive BI,especially those who developed ENL,followed by patients who had neuritis and RR.Among reaction-free patients,81.9%were ML Flow positive,however,the differences were not statistically significant compared to reactional patients(P=0.45).Conclusions:MB and PB patients treated with R-MDT and U-MDT showed similar frequencies of RR and other clinical manifestations.Positive ML Flow tests were associated with MB leprosy and BI positivity.However,ML Flow test results at baseline showed limited sensitivity and specificity for predicting the development of leprosy reactions. | Emerith Mayra Hungria Regiane Morillas Oliveira Gerson Oliveira Penna Lúcio Cartaxo Aderaldo Maria Araci de Andrade Pontes Rossilene Cruz Heitor de SáGonçalves Maria Lúcia Fernandes Penna Ligia Regina Franco Sansigolo Kerr Mariane Martins de Araújo Stefani Samira Bührer-Sékula | 2016 | Infectious Diseases of Poverty2016，5，1： | 2 |
| 5 | Tumors of the jugular foramen:diagnosis and management显示文摘 | RAMINA R MANIGLIA J J FERNANDES Y B | 2005 | Neurosurgery2005，57，： | 1 |
| 6 | Beta-galactosidase from a cold-adapted bacterium: purification, characterization and application for lactose hydrolysis显示文摘 | FERNANDES S GEUEKE B DELGADO O | 2002 | Appl Microbiol Biotechnol2002，58，： | 1 |
| 7 | Characterization of Pore Systems in Seal Rocks Using Nitrogen Gas Adsorption Combined with Mercury Injection Capillary Pressure Techniques 显示文摘 | Schmitt M Fernandes C P da Cunha Neto J A B | 2013 | Marine and Petroleum Geology2013，39，1： | 1 |
| 8 | Effects of herbivory by Hyblaea puera ( Hyblaeidae : Lepidop- tera) on litter production in the mangrove on the coast of Brazi- lian Amazonia显示文摘 | FERNANDES M E B NASCIMENTO A A M CARVALHO M L | 2009 | Journal of Tropical Ecology2009，25，3： | 1 |
| 9 | Letter from the society president 显示文摘 | Fernandes P B | 1997 | Journal of Biomolecular Screening1997，2，1： | 1 |
| 10 | Thermal analysis of the defrost cycle in a domestic freezer显示文摘 | Bansal B Fothergill D Fernandes R | 2010 | International Journal of Refrigeration2010，33，3： | 1 |
| 11 | New spirorotenoids from Tephrosia eandida显示文摘 | ANDREI C C VIEIRA P C FERNANDES J B | 2002 | Zeitschrift Ftir Naturforschung C-A Journal of Bioseienees2002，57，56： | 1 |
| 12 | PLL-Less Active Power Fiher Based onOne-cycle Control for Compensating Unbalanced Loads in Three-Phase Four-Wire System显示文摘 | I-Iirve S Chatterjee K Fernandes B G | 2007 | Power Delivery IEEE Trans- actions on2007，22，4： | 1 |
| 13 | Non-genomic progesterone actions in female reproduction显示文摘 | Gellersen B Fernandes MS Bresens JJ | 2009 | Hum Reprod Update2009，15，1： | 1 |
| 14 | Photochemical processes and the environmental impact of petroleum spills 显示文摘 | Nicodem D E Fernandes M C Z Guedes C L B | 1997 | Biogeochemistry1997，39，： | 1 |
| 15 | Evaluation of the thoracolumbar injury classification system in thoracic and lumbar spinal trauma显示文摘 | JOAQUIM A F FERNANDES Y B CAVALCANTE RA | 2011 | Spine(Phila Pa 1976)2011，36，1： | 1 |
| 16 | An immunophe- notypic and molecular study of primary large B-cell lymphoma of bone 显示文摘 | Huebner-Chan D Fernandes B Yang G | 2001 | Mod Pathol2001，14，10： | 1 |
| 17 | A Rac homolog functions downstream of Rasl to control hyphal differentiation and high-temperature growth in the pathogenic fungus Cryptococcus neoformans显示文摘 | Vallim M A Nichols C B Fernandes L | 2005 | Eukaryotic Cell2005，4，6： | 1 |
| 18 | Mathematical model coupling phase transformation and temperature evolution during quenching of steels显示文摘 | Fernandes F M B Denis S Simon A | 1984 | Materials Science and Technology1984，1，10： | 1 |
| 19 | Non-genomic progesterone actions in female reproduction显示文摘 | Gellersen B Fernandes MS Brosens JJ | | 0，，01： | 1 |
| 20 | A new quasi-resonant DC-link PWM inverter using single switch for soft switching 显示文摘 | Jamal J Fernandes B G | 2002 | IEEE Transactions on Power Electronics2002，17，6： | 1 |
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