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| 1 | Complement C3a activates osteoclasts by regulating the PI3K/PDK1/SGK3 pathway in patients with multiple myeloma显示文摘Objective:Myeloma bone disease(MBD)is the most common complication of multiple myeloma(MM).Our previous study showed that the serum levels of C3/C4 in MM patients were significantly positively correlated with the severity of bone disease.However,the mechanism of C3 a/C4 a in osteoclasts MM patients remains unclear.Methods:The formation and function of osteoclasts were analyzed after adding C3 a/C4 a in vitro.RNA-seq analysis was used to screen the potential pathways affecting osteoclasts,and the results were verified by Western blot,q RT-PCR,and pathway inhibitors.Results:The osteoclast area per view induced by 1μg/m L(mean±SD:50.828±12.984%)and 10μg/m L(53.663±12.685%)of C3 a was significantly increased compared to the control group(0μg/m L)(34.635±8.916%)(P<0.001 and P<0.001,respectively).The relative m RNA expressions of genes,OSCAR/TRAP/RANKL/cathepsin K,induced by 1μg/m L(median:5.041,3.726,1.638,and 4.752,respectively)and 10μg/m L(median:5.140,3.702,2.250,and 5.172,respectively)of C3 a was significantly increased compared to the control group(median:3.137,2.004,0.573,and 2.257,respectively)(1μg/m L P=0.001,P=0.003,P<0.001,and P=0.008,respectively;10μg/m L:P<0.001,P=0.019,P<0.001,and P=0.002,respectively).The absorption areas of the osteoclast resorption pits per view induced by 1μg/m L(mean±SD:51.464±11.983%)and 10μg/m L(50.219±12.067%)of C3 a was also significantly increased(33.845±8.331%)(P<0.001 and P<0.001,respectively)compared to the control.There was no difference between the C4 a and control groups.RNA-seq analysis showed that C3 a promoted the proliferation of osteoclasts using the phosphoinositide 3-kinase(PI3 K)signaling pathway.The relative expressions of PIK3 CA/phosphoinositide dependent kinase-1(PDK1)/serum and glucocorticoid inducible protein kinases(SGK3)genes and PI3 K/PDK1/p-SGK3 protein in the C3 a group were significantly higher than in the control group.The activation role of C3 a in osteoclasts of MM patients was reduced by the SGK inhibitor(EMD638683).Conclusions:C3 a activated osteoclasts by regulating the PI3 K/PDK1/SGK3 pathways in MM patients,which was reduced using a SGK inhibitor.Overall,our results identified potential therapeutic targets and strategies for MBD patients。 | Fengjuan Jiang Hui Liu Fengping Peng Zhaoyun Liu Kai Ding Jia Song Lijuan Li Jin Chen Qing Shao Siyang Yan Kim De Veirman Karin Vanderkerken Rong Fu | 2021 | Cancer Biology & Medicine2021，18，3： | 3 |
| 2 | Molecular phylogeny of methylotrophs in a deep-sea sediment from a tropical west Pacific warm pool 显示文摘 | Wang Peng Wang Fengping Xu Meixiang | 2004 | FEMS Microbiol Ecol2004，47，： | 1 |
| 3 | Molecular Survey of Sulphate-Reducing Bacteria in the Deep-Sea Sediments of the West Pacific Warm Pool显示文摘The sulfate-reducing bacteria(SRB) community in the deep-sea sediments of the west Pacific Warm Pool(WP) was surveyed by molecular phylogenetic analyses using primers targeting the 16S rRNA gene fragments of SRB. Specific 16S rRNA gene libraries from five sediment layers(1-cm,3-cm,6-cm,10-cm and 12-cm layer) of the 12-cm core of WP-0 were constructed. The clones in the five libraries were differentiated by restriction fragment length polymorphism(RFLP) and representative clones were selected to sequence. It was found that the clones fell into four groups,which were closest related to Desulfotomaculum,Desul-facinum,Desulfomonile and Desulfanuticus. Desulfacinum-like clones were only detected in the upper layers of the sediment core,whereas Desulfomonile-like clones were only present in the deeper layers. Fluorescence in situ hybridization(FISH) was further carried out to visualize and count the SRB and bacteria in the five sediment layers. It was found that SRB constituted only a small proportion of the bacteria community(0.34%-1.95%) ,it had the highest content in the 3-cm layer(1.95%) and had a depth-related decreasing tendency along the 12-cm core. | WANG Peng XIAO Xiang ZHANG Haiyan WANG Fengping | 2008 | Journal of Ocean University of China2008，7，3： | 1 |
| 4 | Phylogenetic analysis of Archaea in the deep-sea sediments of west Pacific Warm Pool显示文摘 | Peng Wang Xiang Xiao Fengping Wang | 2005 | Extremophiles2005，，3： | 1 |
| 5 | Application of three‐dimensional reconstruction and visible simulation technique in reoperation of hepatolithiasis显示文摘 | Aowen Xie Chihua Fang Yanpeng Huang Yinfang Fan Jiahui Pan Fengping Peng | 2013 | J Gastroenterol Hepatol2013，，2： | 1 |
| 6 | Dual-wavelength single-longitudinal-mode Tm-doped fiber laser using PM-CMFBG显示文摘 | Yan Fengping Peng Wanjing Liu Shuo | 2015 | IEEE Photon Technol Lett2015，27，9： | 1 |
| 7 | Solution phase parallel synthesis of 4-aminophenyl ethers using a carboxyl-functionalized ionic liquid as support 显示文摘 | Peng Yanqing Yi Fengping Song Gonghua | 2005 | Monatshefte fur Chemie2005，136，： | 1 |
| 8 | Metal-organic frameworks based single-atom catalysts for advanced fuel cells and rechargeable batteries显示文摘The next-generation energy storage systems such as fuel cells,metal-air batteries,and alkali metal(Li,Na)-chalcogen(S,Se)batteries have received increasing attention owing to their high energy density and low cost.However,one of the main obstacles of these systems is the poor reaction kinetics in the involved chemical reactions.Therefore,it is essential to incorporate suitable and efficient catalysts into the cell.These years,single-atom catalysts(SACs)are emerging as a frontier in catalysis due to their maximum atom efficiency and unique reaction selectivity.For SACs fabrication,metal-organic frameworks(MOFs)have been confirmed as promising templates or precursors due to their high metal loadings,structural adjustability,porosity,and tailorable catalytic site.In this review,we summarize effective strategies for fabricating SACs by MOFs with corresponding advanced characterization techniques and illustrate the key role of MOFs-based SACs in these batteries by explaining their reaction mechanisms and challenges.Finally,current applications,prospects,and opportunities for MOFs-based SACs in energy storage systems are discussed. | Yifei Wu Peng Hu Fengping Xiao Xiaoting Yu Wenqi Yang Minqi Liang Ziwei Liang Aixin Zhu | 2023 | Journal of Energy Chemistry2023，，5： | 0 |
| 9 | Analysis of Transmission Characteristics of Tilted Long Period Fiber Gratings With Full Vector Complex Coupled Mode Theory显示文摘把联合模式理论基于完整的向量建筑群,详细分析在倾斜的长时期纤维栅栏的传播光谱特征上被做。新传播山峰被观察,它在每座传播山峰的长波长方面旁边位于正常长时期纤维栅栏的传播光谱。新兴的传播山峰对两个相当敏感栅栏倾斜了角度并且包围折射索引,和相应关系被讨论。而且,一个新奇察觉到特征关于倾斜的长时期纤维栅栏被调查,它与传播有关是反响的波长和山峰振幅。 | Qi LI Fengping YAN Peng LIU Wanjing PENG Guolu YIN Ting FENG | 2012 | Photonic Sensors2012，2，2： | 0 |
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