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| 1 | Core Metabolic Features and Hot Origin of Bathyarchaeota显示文摘The archaeal phylum Bathyarchaeota comprises highly diversified subgroups and is considered to be one of the most abundant microorganisms on earth. The metabolic features and evolution of this phylum still remain largely unknown. In this article, a comparative metabolic analysis of 15 newly reconstructed and 36 published metagenomic assembled genomes (MAGs) spanning 10 subgroups was performed, revealing the core metabolic features of Bathyarchaeota—namely, protein, lipid, and benzoate degradation;glycolysis;and the Wood–Ljungdahl (WL) pathway, indicating an acetyl-CoA-centralized metabolism within this phylum. Furthermore, a partial tricarboxylic acid (TCA) cycle, acetogenesis, and sulfur-related metabolic pathways were found in specific subgroups, suggesting versatile metabolic capabilities and ecological functions of different subgroups. Intriguingly, most of the MAGs from the Bathy-21 and -22 subgroups, which are placed at the phylogenetic root of all bathyarchaeotal lineages and likely represent the ancient Bathyarchaeota types, were found in hydrothermal environments and encoded reverse gyrase, suggesting a hyperthermophilic feature. This work reveals the core metabolic features of Bathyarchaeota, and indicates a hot origin of this archaeal phylum. | Xiaoyuan Feng Yinzhao Wang Rahul Zubin Fengping Wang | 2019 | Engineering2019，5，3： | 7 |
| 2 | Ultra-stable super-resolution fluorescence cryo-microscopy for correlative light and electron cryo-microscopy显示文摘Remarkable progress in correlative light and electron cryo-microscopy(cryo-CLEM) has been made in the past decade. A crucial component for cryo-CLEM is a dedicated cryo-fluorescence microscope(cryo-FM). Here, we describe an ultra-stable superresolution cryo-FM that exhibits excellent thermal and mechanical stability. The temperature fluctuations in 10 h are less than0.06 K, and the mechanical drift over 5 h is less than 200 nm in three dimensions. We have demonstrated the super-resolution imaging capability of this system(average single molecule localization accuracy of ~13.0 nm). The results suggest that our system is particularly suitable for long-term observations, such as single molecule localization microscopy(SMLM) and cryogenic super-resolution correlative light and electron microscopy(csCLEM). | Xiaojun Xu Yanhong Xue Buyun Tian Fengping Feng Lusheng Gu Weixing Li Wei Ji Tao Xu | 2018 | Science China(Life Sciences)2018，61，11： | 3 |
| 3 | Comprehensive test stand for high-intensity cyclotron development显示文摘A comprehensive test stand for high-intensity cyclotron development,a 10 MeV 430 μA cyclotron,has been successfully constructed at China Institute of Atomic Energy (CIAE) for the experimental verification of the viability of a 100 MeV high-intensity cyclotron (CYCIAE-100) being constructed at CIAE.The verification includes the overall design technology,detail design and engineering technology for the crucial parts of the machine,e.g.main magnet and beam diagnostics.It will also pave the way for future enhancement of the beam intensity.The test stand consists of a H-ion source,axial injection system,central region,main magnet and coil,RF resonator,stripping extraction,and auxiliary systems,which include the RF power amplifier,high voltage and DC power supplies,electric apparatus,beam diagnostics,control,vacuum,water cooling,pneumatic system,and safety interlock.This paper presents the work regarding the design and sub-system development of the test sand,and the results of the beam commissioning of the machine. | ZHANG TianJue LI ZhenGuo CHU ChengJie XING JianSheng GUAN FengPing ZHONG JunQing JI Bin GE Tao YIN ZhiGuo HOU ShiGang PAN GaoFeng YAO HongJuan LU YinLong WANG ZhenHui WU LongCheng LIN Jun JIA XianLu WEI SuMin WEN LiPeng WANG Feng XIA Le CAI HongRu XIE HuaiDong CHEN RongFan ZHANG Yan ZHANG SuPing LIU GengShou ZOU Jian AN ShiZhong YANG JianJun BI YuanJie YANG Fang | 2011 | Chinese Science Bulletin2011，56，3： | 3 |
| 4 | Cryogenic superresolution correlative light and electron microscopy of vitreous sections显示文摘Fluorescence microscopy and electron microscopy complement each other as the former provides labelling and localisation of specific molecules and target structures while the latter possesses excellent revolving power of fine structure in context.These two techniques can combine as correlative light and electron microscopy(CLEM)to reveal the organisation of materials within the cell.Frozen hydrated sections allow microscopic observations of cellular components in situ in a near-native state and are compatible with superresolution fluorescence microscopy and electron tomography if sufficient hardware and software support is available and a well-designed protocol is followed.The development of superresolution fluorescence microscopy greatly increases the precision of fluorescence annotation of electron tomograms.Here,we provide detailed instructions on how to perform cryogenic superresolution CLEM on vitreous sections.From fluorescence-labelled cells to high pressure freezing,cryoultramicrotomy,cryogenic single-molecule localisation microscopy,cryogenic electron tomography and image registration,electron tomograms with features of interest highlighted by superresolution fluorescence signals are expected to be obtained. | Buyun Tian Maoge Zhou Fengping Feng Xiaojun Xu Pei Wang Huiqin Luan Wei Ji Yanhong Xue Tao Xu | 2022 | Biophysics Reports2022，8，4： | 0 |
| 5 | Analysis of Transmission Characteristics of Tilted Long Period Fiber Gratings With Full Vector Complex Coupled Mode Theory显示文摘把联合模式理论基于完整的向量建筑群,详细分析在倾斜的长时期纤维栅栏的传播光谱特征上被做。新传播山峰被观察,它在每座传播山峰的长波长方面旁边位于正常长时期纤维栅栏的传播光谱。新兴的传播山峰对两个相当敏感栅栏倾斜了角度并且包围折射索引,和相应关系被讨论。而且,一个新奇察觉到特征关于倾斜的长时期纤维栅栏被调查,它与传播有关是反响的波长和山峰振幅。 | Qi LI Fengping YAN Peng LIU Wanjing PENG Guolu YIN Ting FENG | 2012 | Photonic Sensors2012，2，2： | 0 |
| 6 | Advances in the Immunotherapeutic Potential of Isocitrate Dehydrogenase Mutations in Glioma显示文摘Isocitrate dehydrogenase(IDH)is an essential metabolic enzyme in the tricarboxylic acid cycle(TAC).The high mutation frequency of the IDH gene plays a complicated role in gliomas.In addition to affecting gliomas directly,mutations in IDH can also alter their immune microenvironment and can change immune-cell function in direct and indirect ways.IDH mutations mediate immune-cell infiltration and function by modulating immune-checkpoint gene expression and chemokine secretion.In addition,IDH mutation-derived D2-hydroxyglutarate can be absorbed by surrounding immune cells,also affecting their functioning.In this review,we summarize current knowledge about the effects of IDH mutations as well as other gene mutations on the immune microenvironment of gliomas.We also describe recent preclinical and clinical data related to IDH-mutant inhibitors for the treatment of gliomas.Finally,we discuss different types of immunotherapy and the immunotherapeutic potential of IDH mutations in gliomas. | Feng Tang Zhiyong Pan Yi Wang Tian Lan Mengyue Wang Fengping Li Wei Quan Zhenyuan Liu Zefen Wang Zhiqiang Li | 2022 | Neuroscience Bulletin2022，38，9： | 0 |
| 7 | Correlation of PCSK1 with nonalcoholic fatty liver disease in a Han Chinese population:a case-control observational study显示文摘Objective: This study aimed to investigate the association between single-nucleotide polymorphisms (SNPs) ofPCSK1 (proprotein convertase subtilisin/kexin type 1) related to obesity and nonalcoholic fatty liver disease (NAFLD).Methods: In this case-control observational study, four candidate SNPs (rs6234, rs155971, rs6232, rs3811951) ofPCSK1 were genotyped in 732 NAFLD patients and 823 healthy control participants, all of whom were of ethnic Han Chinese descent. All participants came from Shanghai, China, and joined our study during 2015 to 2016. The frequencies of each allele and genotype, paired linkage disequilibrium, and haplotype were calculated on the SHEsis platform. In addition to SHEsis, five different genetic models (codominant, dominant, recessive, overdominant, and log-additive) were employed to identify the correlation between genotype frequency and NAFLD. This study was approved by the Medical Ethics Committee of Shanghai University of Traditional Chinese Medicine (approved No. 2017LCSY069).Results: In a comparison of NAFLD patients and healthy participants, none of the fourPCSK1 SNPs were significantly correlated with the occurrence of NAFLD (P>0.05), in either genotypic or allelic distribution. The recessive model of rs3811951 appeared to show a correlation (odds ratio=1.077;95% confidence interval=0.924-1.256;P=0.04), but there was no statistical significance after Bonferroni correction (Pcorr>0.0125).Conclusions: Four obesity-relatedPCSK1 SNPs (rs6234, rs155971, rs6232, rs3811951) showed no significant correlation with the development of NAFLD in a Han Chinese population. | Xiao Yang Fan Yuan Na Wu Decheng Ren Liangjie Liu Yan Bi Lei Ji Ke Han Mofan Feng Kai Su Fengping Yang Xi Wu Qing Lu Siran Yue Fengyan Jiang Ruirui Wang Guang He Baocheng Liu Xingwang Li Yi Shi | 2022 | Journal of Bio-X Research2022，5，3： | 0 |
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