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| 1 | Net energy of corn,soybean meal and rapeseed meal in growing pigs显示文摘Background: Two experiments were conducted to estimate the net energy(NE) of corn, soybean meal, expel er-pressed rapeseed meal(EP-RSM) and solvent-extracted rapeseed meal(SE-RSM) using indirect calorimetry and to validate the NE of these four ingredients using pig growth performance.Methods: In Exp.1, 24 barrows(initial BW = 36.4 ± 1.6 kg) were allotted to 1 of 4 diets which included a corn basal diet,a corn-soybean meal basal diet and two rapeseed meal diets containing 20% EP-RSM(9.5% ether extract) or SE-RSM(1.1% ether extract) substituted for corn and soybean meal. The design allowed the calculation of NE values of corn,soybean meal and rapeseed meals according to the difference method. In Exp.2, 175 growing pigs(initial BW = 36.0± 5.2 kg) were fed 1 of 5 diets for 28 d, with five pigs per pen and seven replications(pens) per treatment in order to validate the measured energy values. Diets were a corn-soybean meal diet and four diets including 10% or 20% EP-RSM and 10% or 20% SE-RSM.Results: The NE of corn, soybean meal, EP-RSM and SE-RSM were 12.46, 11.34, 11.71 and 8.83 MJ/kg DM, respectively. The NE to ME ratio of corn(78%) was similar to tabular values, however, the NE to ME ratios of soybean meal(70%) and rapeseed meal(76%) were greater than tabular values. The greater NE value in EP-RSM than in SE-RSM is consistent with its higher EE content. Increasing EP-RSM or SE-RSM did not affect the growth performance of pigs and the caloric efficiency of NE was comparable for al diets.Conclusions: The NE of EP-RSM was similar to soybean meal, and both were greater than SE-RSM. The DE, ME and NE values measured in Exp.1 are confirmed by results of Exp. 2 with comparable caloric efficiencies of DE, ME or NE for all diets. | Zhongchao Li Yakui Li Zhiqian Lv Hu Liu Jinbiao Zhao Jean Noblet Fenglai Wang Changhua Lai Defa Li | 2017 | Journal of Animal Science and Biotechnology2017，8，4： | 7 |
| 2 | Soil microorganisms alleviate the allelopathic effect of Eucalyptus grandis × E. urophylla leachates on Brassica chinensis显示文摘Soil microbes may be critical players in determining the allelopathic potential of some plants. Low levels of plant community biodiversity in Eucalyptus plantations have been attributed to the allelopathic potential of these tree species. The role of soil microbes in the allelopathic effect of leaf leachates of the hybrid tree Eucalyptus grandis×E.urophylla, was tested in Petri dish assays with Brassica chinensis as a receiver plant. Soils were collected from either a local garden(soil A) or a Eucalyptus plantation(soil B) and half of each soil was sterilized to remove microbes.These soils were then treated with E. grandis×E. urophylla leachates for 0–72 h. Seed germination of B. chinensis was significantly inhibited in soils treated with leaf leachates relative to untreated soils. The inhibitory effect of the leaf leachates was more pronounced in sterilized soils. Total phenolic content was obvious lower in nonsterile leachatetreated soils than in sterile soils. Biomass of B. chinensis was negatively correlated with the total phenolic content in soils.Our findings suggest that soil microbes can alleviate the allelopathic potential of Eucalyptus and thereby its negative impact on plant growth. | Fenglai Lu Lilang Zheng Yueyuan Chen Dianpeng Li Rensen Zeng Huashou Li | 2017 | Journal of Forestry Research2017，28，6： | 6 |
| 3 | Methodologies on estimating the energy requirements for maintenance and determining the net energy contents of feed ingredients in swine:a review of recent work显示文摘In the past two decades, a considerable amount of research has focused on the determination of the digestible(DE) and metabolizable energy(ME) contents of feed ingredients fed to swine. Compared with the DE and ME systems, the net energy(NE) system is assumed to be the most accurate estimate of the energy actually available to the animal. However, published data pertaining to the measured NE content of ingredients fed to growing pigs are limited. Therefore, the Feed Data Group at the Ministry of Agricultural Feed Industry Centre(MAFIC) located at China Agricultural University has evaluated the NE content of many ingredients using indirect calorimetry. The present review summarizes the NE research works conducted at MAFIC and compares these results with those from other research groups on methodological aspect. These research projects mainly focus on estimating the energy requirements for maintenance and its impact on the determination, prediction, and validation of the NE content of several ingredients fed to swine. The estimation of maintenance energy is affected by methodology, growth stage,and previous feeding level. The fasting heat production method and the curvilinear regression method were used in MAFIC to estimate the NE requirement for maintenance. The NE contents of different feedstuffs were determined using indirect calorimetry through standard experimental procedure in MAFIC. Previously generated NE equations can also be used to predict NE in situations where calorimeters are not available. Although popular, the caloric efficiency is not a generally accepted method to validate the energy content of individual feedstuffs. In the future,more accurate and dynamic NE prediction equations aiming at specific ingredients should be established, and more practical validation approaches need to be developed. | Zhongchao Li Hu Liu akui Li Zhiqian Lv Ling Liu Changhua Lai Junjun Wang Fenglai Wang Defa Li Shuai Zhang | 2018 | Journal of Animal Science and Biotechnology2018，9，3： | 6 |
| 4 | Pyrroloquinoline quinone regulates the redox status in vitro and in vivo of weaned pigs via the Nrf2/HO-1 pathway显示文摘Background:Oxidative stress is a main cause of piglet gut damage and diarrhea.Pyrroloquinoline quinone(PQQ),is a novel redox cofactor with antioxidant properties.However,the effect and mechanism that PQQ supplementation decreases oxidative injury in weaned pigs is not understood.Therefore,the aim of this study is to confirm the effect of PQQ on regulating redox status in weaned pigs and the mechanism for antioxidant function by porcine intestinal epithelial cell line(IPEC-J2)challenged with H_(2)O_(2).Results:Experiment 1,144 Duroc×Landrace×Yorkshire pigs(weaned at 28 d)were allocated to four groups:received a basal diet(control)and diets supplemented with 0.15%,0.30%and 0.45%PQQ,respectively.On d 28,growth performance,diarrhea incidence and redox factors were measured.Experiment 2,IPEC-J2 were treated with or without PQQ in the presence or absence of H_(2)O_(2)for indicated time points.Experiment 3,IPEC-J2 were transfected with or without Nrf2 siRNA,then treated according to Experiment 2.The cell viability,redox factors,protein of tight junctions and Nrf2 pathway were determined.In vivo,PQQ supplementation demonstrated dose-related improvements in average daily gain,and gain to feed ratio(Linear P<0.05).During d 0–28,compared to controls,0.45%PQQ supplementation for pigs decreased diarrhea incidence and MDA content in liver and jejunum,and increased concentration of SOD in liver;0.3%PQQ supplementation decreased ileal and liver MDA concentration;and 0.15%PQQ supplementation decreased ileal MDA concentration(P<0.05).In vitro,compared to cells cultured with H_(2)O_(2),pre-treatment with PQQ increased cell viability,tight junction proteins expression including ZO-1,ZO-2,Occludin and Claudin-1;and decreased ROS concentration and level of Caspase-3(P<0.05);as well as upregulated the ratio of Bcl-2 to Bax and protein expression of nuclear Nrf2,HO-1.Notably,Nrf2 knockdown by transfection with Nrf2 siRNA largely abrogated the positive effects of PQQ pretreatment on H_(2)O_(2)-induced intracellular changes.Conclusions:PQQ administration attenuated oxidative stress in weaned pigs which is associated with activation of Nrf2/HO-1 pathway. | Caiyun Huang Zijuan Fan Dandan Han Lee J.Johnston Xi Ma Fenglai Wang | 2021 | Journal of Animal Science and Biotechnology2021，12，4： | 4 |
| 5 | Effects of dietary ratio of n-6 to n-3 polyunsaturated fatty acids on immunoglobulins, cytokines, fatty acid composition, and performance of lactating sows and suckling piglets显示文摘This experiment was conducted to investigate the effects of dietary ratios of n-6:n-3 polyunsaturated fatty acids (PUFA) on the performance of lactating sows and their piglets. Thirty pregnant Landrace sows were assigned to one of three treatments from d 108 of gestation until weaning (26-29 d) and were fed diets containing different ratios of n-6:n-3 PUFA including 3:1, 9:1 and 13:1. The effects on sow and litter production traits were examined together with an assessment of sow body condition. No differences were detected among the treatments for the daily feed intake of sows or changes in sow weight and back-fat levels during lactation (P > 0.05). Litter size at d 14 and d 21 were tended to increase in 3:1 treatment compared with 9:1 and 13:1 treatments (P < 0.10). Litter weight gain (1.77 kg/d) from d 0 to d 14 was tended to increase in 9:1 groups compared with the other two treatments (P < 0.10). A significant difference was observed for the content of α -linolenic acid, total n-3 PUFA, and the ratio of n-6:n-3 PUFA in the colostrum, milk, and piglets plasma (P < 0.01). The effects of different ratios of n-6:n-3 PUFA in sow diets on colostrum, milk, and piglet plasma immunoglobulin concentrations are studied. No difference was observed among treatments in the concentrations of IgM, and IgA in colostrum (P > 0.05). A great significant difference for IgG concentration was observed among 3 group in colostrum. A great significant difference for IgA, and IgM (P < 0.01) concentrations in piglet plasma at d14 and a significant difference for IgG(P < 0.05) was observed at d14. Furthermore, at d 21 of lactation, piglet plasma IgG and IgA concentration were greater in 3:1 compared with 13:1 group (P < 0.01). In summary, the current study demonstrated that altering the ratio of n-6:n-3 PUFA in lactating sow diet had an effect on the immune component including immunoglobulin and cytokines, and it tended to increase the litter average daily gain and improve the immune status of piglets when dietary ratio of n-6:n-3 PUFA was 9:1. | Wei Yao Jie Li Jun jun Wang Weiliang Zhou Qingbo Wang Rongchang Zhu Fenglai Wang Phil Thacker | 2013 | Journal of Animal Science and Biotechnology2013，4，2： | 4 |
| 6 | 比利时水下文化遗产立法对我国《水下文物保护管理条例》修订的参考意义显示文摘《中华人民共和国水下文物保护管理条例》(以下简称“《条例》”)正在修订当中,与此同时,我国正在积极考虑加入《保护水下文化遗产公约》(以下简称“《公约》”)。为了使《条例》尽量符合《公约》的精神,在《条例》的修订过程中,《公约》的规定是不可避免的考虑因素。《公约》至今为止有60 个缔约国,而比利时是少数根据《公约》规定,重新订立相关法律的缔约国之一。虽然我国还没有加入《公约》,但是此时修订《条例》不可能不考虑《公约》的影响。了解缔约国在加入《公约》后对其本国立法作出何种调整,以及作出调整的原因,对我国目前正在进行的《条例》修订工作有着重要的参考意义。 | 林蓁 HUANG Yuxin LIN Fenglai | 2019 | 中华海洋法学评论2019，15，1： | 3 |
| 7 | Experimental studies on behavior of fully grouted reinforced-concrete masonry shear walls显示文摘An experimental study is conducted on fully grouted reinforced masonry shear walls(RMSWs) made from concrete blocks with a new configuration. Ten RMSWs are tested under reversed cyclic lateral load to investigate the influence of different reinforcements and applied axial stress values on their seismic behavior. The results show that flexural strength increases with the applied axial stress, and shear strength dominated by diagonal cracking increases with both the amount of horizontal reinforcement and applied axial stress. Yield displacement, ductility, and energy dissipation capability can be improved substantially by increasing the amount of horizontal reinforcement. The critical parameters for the walls are derived from the experiment: displacement ductility values corresponding to 15% strength degradation of the walls reach up to 2.6 and 4.5 in the shear and flexure failure modes, respectively; stiffness values of flexure- and shear-dominated walls rapidly degrade to 17% – 19% and 48% – 57% of initial stiffness at 0.50 D max(displacement at peak load). The experiment suggests that RMSWs could be assigned a higher damping ratio(~14%) for collapse prevention design and a lower damping value(~7%) for a fully operational limit state or serviceability limit state. | Zhao Yan Wang Fenglai | 2015 | Earthquake Engineering and Engineering Vibration2015，14，4： | 3 |
| 8 | Effects of dietary fiber content and different fiber-rich ingredients on endogenous loss of fat and fatty acids in growing pigs显示文摘Background: Determination of the endogenous loss of fat(ELF) is used to adjust for the estimation of true total tract digestibility(TTTD) of fat in diets and ingredients.Any factor which affected ELF may further affect the digestibility of fat,including sources and concentrations of fat and fiber in the diet.There are some reports of determining the ELF using regression methods based on different levels of fat intake,while reports on effects of dietary fiber content and different fiber-rich ingredients in pig diets on ELF are very limited.Therefore,the objective of this study was to determine the effects of dietary fiber content and different fiber-rich ingredients on endogenous losses of fat and fatty acids at the end of ileum and throughout the entire intestinal tract in growing pigs.Methods: In Exp.1,the effect of fiber content on endogenous loss of fat was determined using six growing pigs(Duroc × Landrace × Yorkshire;27.6 ± 2.4 kg),fitted with a T-cannula at the end of ileum.The experimental design was a 6 × 6 complete Latin square design with six periods of feeding and six diets.The six experimental fat-free diets were formulated to include graded levels of neutral detergent fiber(NDF)(0,40,80,120,160 and 200 g/kg) and soybean hull(SH) was the only fiber source,providing 0,75,150,225,300 and 375 g/kg,respectively.Chromic oxide was included at4 g/kg in all diets as an indigestible marker.In Exp.2,six crossbred growing barrows(27.6 ± 1.6 kg) were used and the experimental design was the same as for Exp.1.The six fat-free diets were formulated to include six common fiber-rich ingredients and the concentration of NDF was 100 g/kg.The six fiber-rich ingredients were defatted rice bran(DRB),sugar beet pulp(SBP),rice hull(RH),corn germ meal(CGM),SH and wheat bran(WB) and they were fed at represented250,270,145,250,170 and 280 g/kg in the diet,respectively.Results: In Exp.1,the endogenous loss of fatty acids profile did not change as dietary NDF increased in growing pigs.The endogenous losses of fat,C16:0,C18:0,C18:1,C18:2,total unsaturated fatty acids(UFA) and total saturated fatty acids(SFA) in growing pigs at the end of ileum and throughout the entire intestinal tract increased linearly as NDF content of diets increased.The endogenous losses of fat,as well as C16:0 and C18:0 throughout the entire intestinal tract also increased quadratically as NDF content of diets increased.The ELF increased from 0.71 to 3.14 g/kg of dry matter intake(DMI) and 0.56 to 8.21 g/kg DMI at the end of ileum and throughout the entire intestinal tract in growing pigs,respectively.The ELF occurred in the hindgut except for the growing pigs fed 0 and 4% NDF in their diets.The endogenous losses of C16:0 and UFA occurred primarily in the upper regions of the gut and the greatest endogenous losses of C18:0 occurred in the hindgut.The endogenous losses of fat,individual SFA and total SFA throughout the entire intestinal tract were much greater than that at the end of ileum.However,the endogenous losses of individual UFA and total UFA were less throughout the intestinal tract than at the end of ileum.In Exp.2,the endogenous losses of fat at the end of ileum were greater in growing pigs fed CGM or WB diets.The endogenous loss of fatty acids profile changed to a slight degree at the end of ileum that the endogenous loss of UFA(particularly C18:1 and C18:2) in growing pigs fed CGM or WB diets were greater(P < 0.01) than that for the other four diets.The greatest(P < 0.01) endogenous loss of SFA(particularly C18:0) was in growing pigs fed the RH diet.The endogenous losses of fat,C16:0,C18:0 and SFA over the entire intestinal tract were much greater in growing pigs fed CGM or WB diets,whereas the lowest values were in growing pigs fed DRB diet.The ELF at the end of ileum in growing pigs fed CGM or WB diets were 3.50 or 4.17 g/kg DMI,respectively,and the ELF over the entire intestinal tract was 7.23 or 7.38 g/kg DMI.The contribution in percentage of ELF in the upper gut was greater than that in the hindgut of growing pigs fed DRB and RH diets,while the ELF in the upper gut and hindgut were equal in growing pigs fed SBP,CGM and WB diets.On the whole,the endogenous losses of C18:1 and C18:2 throughout the entire intestinal tract in growing pigs fed the six fiber-rich ingredients diets were less than losses at the end of ileum,whereas the endogenous loss of fat,C16:0,C18:0 and SFA were greater throughout the intestinal tract than at the end of ileum.Conclusion: The profile of loss in endogenous fatty acids did not change as dietary NDF increased in growing pigs and the endogenous losses of fatty acids(C16:0,C18:0,C18:1 and C18:2) fat,UFA and SFA increased linearly as NDF content increased in the diets of pigs.The endogenous losses of fat or fatty acids at the end of ileum were greater in growing pigs fed RH,CGM or WB diets.The endogenous losses of fat,fatty acids(C16:0 and C18:0) and SFA were greater over the entire intestinal tract in pigs fed CGM or WB diet,while these values were the lowest in growing pigs fed the DRB diet.The contribution in percentage losses of fat in the upper gut were greater than in the hindgut of growing pigs fed DRB and RH diets,while the contribution of losses of fat in the upper gut and hindgut were equal in growing pigs fed SBP,CGM and WB diets.In addition,the endogenous loss of individual or total UFA was less over the entire intestinal tract of growing pigs fed fiber diets than that at the end of ileum,and the greatest endogenous losses of fat,individual or total SFA were over the entire intestinal tract.Therefore,differences in fiber content and the nature of fiber-rich ingredients in diets of pigs have different effects to the endogenous losses of fat or fatty acids.Considering the requirement of fat or fatty acids of pigs,careful attention must be paid that the endogenous losses of fat and fatty acids when fiber ingredients are used in diets of pigs. | Yifan Chen Zhenyu Wang Jian Ding Dongxu Ming Wenhui Wang Zhaoning Jiang Ling Liu Fenglai Wang | 2019 | Journal of Animal Science and Biotechnology2019，10，3： | 2 |
| 9 | Machine learning for detecting mesial temporal lobe epilepsy by structural and functional neuroimaging显示文摘Mesial temporal lobe epilepsy(mTLE),the most common type of focal epilepsy,is associated with functional and structural brain alterations.Machine learning(ML)techniques have been successfully used in discriminating mTLE from healthy controls.However,either functional or structural neuroimaging data are mostly used separately as input,and the opportunity to combine both has not been exploited yet.We conducted a multimodal ML study based on functional and structural neuroimaging measures.We enrolled 37 patients with left mTLE,37 patients with right mTLE,and 74 healthy controls and trained a support vector ML model to distinguish them by using each measure and the combinations of the measures.For each single measure,we obtained a mean accuracy of 74%and 69%for discriminating left mTLE and right mTLE from controls,respectively,and 64%when all patients were combined.We achieved an accuracy of 78%by integrating functional data and 79%by integrating structural data for left mTLE,and the highest accuracy of 84%was obtained when all functional and structural measures were combined.These findings suggest that combining multimodal measures within a single model is a promising direction for improving the classification of individual patients with mTLE. | Baiwan Zhou Dongmei An Fenglai Xiao Running Niu Wenbin Li Wei Li Xin Tong Graham J Kemp Dong Zhou Qiyong Gong Du Lei | 2020 | Frontiers of Medicine2020，14，5： | 2 |
| 10 | Effects of the Ratio of n-6∶n-3 Polyunsaturated Fatty Acids in the Maternal Diet on Immunoglobulins,Fatty Acid Composition,and Performance of Lactating Sows and Suckling Piglets under Conditions of Low Maternal Energy Intake显示文摘This experiment was conducted to investigate the effects of different ratios of n-6 ∶ n-3 polyunsaturated fatty acids (PUFA) in sow diets on the performance of lactating sows and their piglets at low digestible energy intakes. Twenty-one pregnant Landrace sows were assigned to one of three diets from day 108 of gestation until weaning (26 to 29 d) ,containing n-6∶n-3 PUFA ratios of 3 ∶1,8 ∶1 and 11 ∶1. The effects on sow and litter production traits and on sow body condition were examined. There were no differences among treatments in daily feed intakes or in changes in sow weight and back-fat levels during lactation. Litter size,litter weight at birth and weaning and litter average daily weight gain were also unaffected by treatment. As expected, large differences were observed in n-6 and n-3 fatty acids in the colostrum and plasma of sows and piglets (P < 0. 01) . The ratio of n6 ∶ n-3 PUFA the diet was positively correlated with those in colostrum,sow plasma and piglet plasma (R 2= 0. 55,0. 80 and 0. 80,respectively) . Sow plasma insulin and IGF-I levels at weaning were unaffected by the treatments. Plasma leptin (P < 0. 05) concentrations were increased in sows fed the diet with a n-6∶n-3 ratio of 8 ∶ 1. Immunoglobulin concentrations in colostrum were not altered by dietary treatment. Plasma IgG concentrations at d 14 were highest in piglets from sows fed the 8 ∶ 1 ratio of n-6∶n-3. Furthermore,this group had the highest IgA concentrations at day 21 of lactation compared with the other two groups (P < 0. 05) . In summary,our study demonstrated that at low digestible energy levels,altering the ratio of n-6 ∶ n-3 PUFA in the diets for lactating sows affected immune components and the fatty acid composition of lactating sows and their piglets. Further studies are needed to examine whether higher levels of fat supplementation than those used in the present study (1. 5%) can successfully enhance performance. | Wei Yao Hongliang Zhao Jie Li Zhijun Wang Yuhuang Han Shuren Dong Yuejiang Wang Fenglai Wang | 2011 | Journal of Animal Science and Biotechnology2011，2，3： | 2 |
| 11 | Recent advances in immunoassays and biosensors for mycotoxins detection in feedstuffs and foods显示文摘Mycotoxins are secondary metabolites produced by fungus.Many mycotoxin species are highly toxic and are frequently found in cereals and feedstuffs.So,powerful detection methods are vital and effective ways to prevent feed contamination.Traditional detection methods can no longer meet the needs of massive,real-time,simple,and fast mycotoxin monitoring.Rapid detection methods based on advanced material and sensor technology are the future trend.In this review,we highlight recent progress of mycotoxin rapid detection strategies in feedstuffs and foods,especially for simultaneous multiplex mycotoxin determination.Immunoassays,biosensors,and the prominent roles of nanomaterials are introduced.The principles of different types of recognition and signal transduction are explained,and the merits and pitfalls of these methods are compared.Furthermore,limitations and challenges of existing rapid sensing strategies and perspectives of future research are discussed. | Runxian Li Yang Wen Fenglai Wang Pingli He | 2022 | Journal of Animal Science and Biotechnology2022，13，2： | 1 |
| 12 | Studies on chemical fingerprints of Siraitia grosvenorii fruits(Luo Han Guo) by HPLC显示文摘 | Fenglai Lu Dianpeng Li Chuanming Fu | 2012 | Journal of Natural Medicines2012，66，1： | 1 |
| 13 | Binding ofpeptide substrate and inhibitors of angiotensin-converting enzyme显示文摘 | CHEUNG H S WANG Fenglai ONDETTI E | 1980 | Journal of Biological Chemistry1980，255，： | 1 |
| 14 | Binding of Pep- tide Substrates and Inhibitors of Angiotensin-converting Enzyme显示文摘 | Cheung Hongson Wang Fenglai Ondetti MA | 1980 | The Journal of Biological Chemistry1980，255，： | 1 |
| 15 | 论海南渔民的口传“更路经”显示文摘专家研究海南渔民口传的“更路经”时,常常将其与《更路簿》混为一谈。实际上,二者既有密切的联系,又有明显的区别。宋元时期,海南渔民在西沙群岛生产作业时,口传“更路经”就已经产生;而纸质版《更路簿》则晚至明代才出现。口传“更路经”里的地名有些被代代流传至今,有些被写进《更路簿》里,成为《更路簿》中地名的重要来源. | 阎根齐 HUANG Yuxin LIN Fenglai | 2019 | 中华海洋法学评论2019，15，1： | 1 |
| 16 | Deployment and Exploration of a Gas Storage Well Pattern Based on the Threshold Radius显示文摘To tackle the problem that wells that are deployed in a specific pattern based on the requirements of gas reservoir development are not suitable for gas storage,we have conducted concentrically circular injection and production simulation experiments for gas storage,discovered the existence of a threshold radius,denoted by Rt,and derived the expression for Rt.Based on the analysis and discussion results,we propose a strategy for deploying gas storage wells in specific patterns.The expression for Rt shows that it is affected by factors such as the gas storage gas production/injection time,the upper pressure limit,the lower pressure limit,the bottomhole flow pressure at the ends of injection and production,the and permeability.The analysis and discussion results show that the Rt of a gas storage facility is much smaller than the Rt for gas reservoir development.In the gas storage facilities in China,the Rt for gas production is less than the Rt for the gas injection,and Rt increases with the difference in the operating pressure and with permeability K.Based on the characteristics of Rt,we propose three suggestions for gas storage well pattern deployment:(1)calculate Rt according to the designed functions of the gas storage facility and deploy the well pattern according to Rt;(2)deploy sparser,large-wellbore patterns in high-permeability areas and denser,small-wellbore patterns in high-permeability areas;and(3)achieve the gas injection well pattern by new drilling,and the gas production well pattern through a combination of the gas injection well pattern and old wells.By assessing a gas storage facility with a perfect well pattern after a number of adjustments,we found that the Rt of the 12 wells calculated in this paper is basically close to the corresponding actual radius,which validates our method.The results of this study provide a methodological basis for well pattern deployment in new gas storage construction. | TANG Ligen ZHU Weiyao ZHU Huayin SUN Chunhui YANG Fenglai WANG Yan Li Xiaorui Li Haiming CHU Guangzhen WANG Jieming KONG Debin YUE Ming LIU Yuwei HUANG Kun | 2021 | Acta Geologica Sinica(English Edition)2021，95，2： | 1 |
| 17 | Integrative analysis of indirect calorimetry and metabolomics profiling reveals alterations in energy metabolism between fed and fasted pigs显示文摘Background: Fasting is a simple metabolic strategy that is used to estimate the maintenance energy requirement where the energy supply for basic physiological functions is provided by the mobilization of body reserves.However, the underlying metabolic components of maintenance energy expenditure are not clear. This study investigated the differences in heat production(HP), respiratory quotient(RQ) and plasma metabolites in pigs in the fed and fasted state, using the techniques of indirect calorimetry and metabolomics.Methods: Nine barrows(45.2 ± 1.7 kg BW) were fed corn-soybean based meal diets and were kept in metabolism crates for a period of 14 d. After 7 d adaptation, pigs were transferred to respiratory chambers to determine HP and RQ based on indirect calorimetry. Pigs were fed the diet at 2,400 k J ME/(kg BW0.6·d) during d 8 to 12. The last 2 d were divided into 24 h fasting and 48 h fasting treatment, respectively. Plasma samples of each pig were collected from the anterior vena cava during the last 3 d(1 d while pigs were fed and 2 d during which they were fasted).The metabolites of plasma were determined by high-resolution mass spectrometry using a metabolomics approach.Results: Indirect calorimetry analysis revealed that HP and RQ were no significant difference between 24 h fasting and 48 h fasting, which were lower than those of fed state(P < 0.01). The nitrogen concentration of urine tended to decrease with fasting(P = 0.054). Metabolomics analysis between the fed and fasted state revealed differences in15 compounds, most of which were not significantly different between 24 h fasting and 48 h fasting. Identified compounds were enriched in metabolic pathways related to linoleic acid metabolism, amino acid metabolism,sphingolipid metabolism, and pantothenate and Co A biosynthesis.Conclusion: These results suggest that the decreases in HP and RQ of growing pigs under fasting conditions were associated with the alterations of linoleic acid metabolism and amino acid metabolism. The integrative analysis also revealed that growing pigs under a 24-h fasting were more appropriate than a 48-h fasting to investigate the metabolic components of maintenance energy expenditure. | Hu Liu Yifan Chen Dongxu Ming Ji Wang Zhen Li Xi Ma Junjun Wang Jaap van Milgen Fenglai Wang | 2018 | Journal of Animal Science and Biotechnology2018，9，3： | 1 |
| 18 | Binding of peptide substrates and inhibitors of angiotensin-converting enzyme显示文摘 | CHEUNG H S WANG Fenglai ONDETTI | 1980 | Biological Chemistry1980，255，2： | 1 |
| 19 | Picard values and normal families of meromorphic functions 显示文摘 | Xu Yan Wu Fenglai Liao Liangwen | 2009 | Proc Roy Soc Edinburgh Sect A2009，139，5： | 1 |
| 20 | Emerging technologies for amino acid nutrition research in the post-genome era显示文摘 | Junjun Wang Guoyao Wu Huaijun Zhou Fenglai Wang | 2009 | Amino Acids2009，，1： | 1 |
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