|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Technology development for rare earth cleaner hydrometallurgy in China显示文摘China is the global leader in rare earth(RE)production using hydrometallurgical processes. Advantageous extraction techniques of rare earths from Baotou mixed rare earth minerals, bastnaesite, and ion-adsorption clays of rare earth deposits have been developed in China.The separation and purification technologies have also achieved rapid development. The industrial application processes for rare earth hydrometallurgy were summarized in the present paper. With the large demands and rapid development of rare earths, the issues of resources and environment are more prominent. This review gives an overview of the main processes that were developed in the past toward greener hydrometallurgy of rare earths in China. Based on the development of the rare earth industry,comprehensive utilization and cleaner production should still be focused on in the future, to support the sustainable development. | Xiao-Wei Huang Zhi-Qi Long Liang-Shi Wang Zong-Yu Feng | 2015 | Rare Metals2015，34，4： | 76 |
| 2 | TGF-β signaling in cancer metastasis显示文摘转变生长因素(TGF )- 发信号的事件是众所周知的控制多样的进程和众多的回答例如房间增长,区别, apoptosis,和移植。在癌症的 TGF- 发信号戏上下文依赖者角色:在 pre 恶意的房间, TGF- 首先作为肿瘤 suppressor 工作,当在癌症的以后的阶段发信号的 TGF- 支持侵略和转移时。最近的研究也建议了在 TGF- 发信号和另外的发信号的小径之间的串音例如河马, Wnt, EGFR/RAS,和 PI3K/AKT 小径,可以实质地贡献我们 TGF- 发信号和癌症的当前的理解。由于 TGF- 的广阔效果, TGF- 和它的下游的发信号部件的封锁提供多重治疗学的机会。因此,为为众多的疾病表明治疗的 anti-TGF- 的眼界显得明亮并且将提供珍贵信息并且想在这药上分子的设计。在这评论,我们集中于最近的卓见进在可以贡献新奇指向癌症的治疗的发展的癌症转移发信号的 TGF- 的规定。 | Feng Xie Li Ling Hans van Dam Fangfang Zhou Long Zhang | 2018 | Acta Biochimica et Biophysica Sinica2018，50，1： | 36 |
| 3 | Screening test for anti-Helicobacter pylori activity of traditional Chinese herbal medicines显示文摘AIM:To evaluate the anti-Helicobacter pylori (H.pylori) activity of 50 traditional Chinese herbal medicines in order to provide the primary evidence for their use in clinical practice.METHODS:A susceptibility test of water extract from 50 selected traditional Chinese herbal medicines for in vitro H.pylori Sydney strain 1 was performed with broth dilution method.Anti-H.pylori activity of the selected Chinese herbal medicines was evaluated according to their minimum inhibitory concentration (MIC).RESULTS:The water extract from Rhizoma Coptidis,Radix Scutellariae and Radix isatidis could significantly inhibit the H.pylori activity with their MIC less than 7.8 mg/mL,suggesting that traditional Chinese herbal medicines have anti-inflammatory and antibacterial effects and can thus be used in treatment of H.pylori infection.CONCLUSION:Rhizoma Coptidis,Radix Scutellariae and Radix isatidis are the potential sources for the synthesis of new drugs against H.pylori. | Feng Ma Ye Chen Jing Li He-Ping Qing Ji-De Wang YaLi Zhang Yang Bai Bei-Guo Long | 2010 | World Journal of Gastroenterology2010，16，44： | 34 |
| 4 | Detection of HBV DNA and its existence status in liver tissues and peripheral blood lymphocytes from chronic hepatitis B patients显示文摘INTRODUCTIONCumulativeclinicalandexperimentalevidenceindicatesthathostimmuneresponsestohepatitisBvirus(HBV)areresponsibleforl... | TANG Ren Xian, GAO Feng Guang, ZENG Ling Yu, WANG Ying Wei and WANG Yu Long | 1999 | World Journal of Gastroenterology1999，5，4： | 27 |
| 5 | ;he regulation of TGF-β/SMAD signaling by protein deubiquitination显示文摘 | Juan Zhang Xiaofei Zhang Feng Xie Zhengkui Zhang Hans van Dam Long Zhang Fangfang Zhou | 2014 | Protein & Cell2014，5，7： | 27 |
| 6 | Tectonic dynamics and correlation of major earthquake sequences of the Xiaojiang and Qujiang-Shiping fault systems, Yunnan, China显示文摘The N-S trending Xiaojiang fault zone and the NW-SE trending Qujiang-Shiping fault zone are adjacent active fault systems and seismogenic zones associated with strong and major earthquakes in Yunnan, China. To understand the interaction of the two fault systems, and its probable influence on earthquake occurrences, this paper conducts a synthetic study based on data of active tectonics, historical earthquakes, relocated small earthquakes, GPS station velocities and focal mechanism resolutions. The study makes several conclusions. (1) The active southward motion of the western side of the Xiaojiang fault zone (i.e. the side of the Sichuan-Yunnan block) has a persistent and intensive effect on the Qujiang-Shiping fault zone. The later fault zone has absorbed and transformed the southward motion of the western side of the former fault zone through dextral strike-slip/shearing as well as transverse shortening/thrusting. (2) Along the Xiaojiang fault zone, the present sinistral strike-slip/shearing rate decreases from 10 and 8 mm/a on the northern, central and central-southern segments to 4 mm/a on the southern segment. The decreased rate has been adjusted in the area along and surrounding the Qujiang-Shiping fault zone through reverse-dextral faulting and distributed shearing and shortening. (3) The tectonic-dynamic relation between the Xiaojiang fault zone and the Qujiang-Shiping fault zone is also manifested by a close correlation of earthquake occurrences on the two fault zones. From 1500 to 1850 a sequence of strong and major earthquakes occurred along the Xiaojiang fault zone and its northern neighbor, the Zemuhe fault zone, which was characterized by gradually accelerating strain release, gradually shortening intervals between M≥7 events, and major releases occurring in the mid to later stages of the sequence. As a response to this sequence, after an 88-year delay, another sequence of 383 years (from 1588 to 1970) of strong and major earthquakes occurred on the Qujiang-Shiping fault zone, and had the same features in accelerating strain release and its temporal course. (4) Since there has been no M≥7 event for 177 years on the Xiaojiang fault zone, the potential risk of a strong or major earthquake occurring on this fault zone in the future should be noticed and studied further. | WEN XueZe DU Fang LONG Feng FAN Jun ZHU Hang | 2011 | Science China Earth Sciences2011，54，10： | 22 |
| 7 | Efficient production of omega-3 fatty acid desaturase (sFat-1)-transgenic pigs by somatic cell nuclear transfer显示文摘Omega-3(ω-3) fatty acid desaturase transgenic pigs may improve carcass fatty acid composition. The use of transgenic pigs is also an excellent large animal model for studying the role of ω-3 fatty acids in the prevention and treatment of coronary heart disease and cancer. Transgenic pigs carrying synthesized fatty acid desaturase-1 gene (sFat-1) from Caenorhabditis briggsae by somatic cell nuclear transfer (SCNT) were produced for the first time in China. Porcine fetal fibroblast cells were transfected with a sFat-1 expression cassette by the liposome-mediated method. Transgenic embryos were reconstructed by nuclear transfer of positive cells into enucleated in vitro matured oocytes. A total of 1889 reconstructed embryos were transferred into 10 naturally cycling gilts. Nine early pregnancies were established, 7 of which went to term. Twenty-one piglets were born. The cloning efficiency was 1.1% (born piglets/transferred embryos). The integration of the sFat-1 gene was confirmed in 15 live cloned piglets by PCR and Southern blot except for 2 piglets. Expression of the sFat-1 gene in 12 of 13 piglets was detected with RT-PCR. The data demonstrates that an efficient system for sFat-1 transgenic cloned pigs was developed, which led to the successful production of piglets expressing the sFat-1 gene. | PAN DengKe1, ZHANG Li1, ZHOU YanRong2, FENG Chong1, LONG Chuan1, LIU Xiao1, WAN Rong3, ZHANG Jian3, LIN AiXing3, DONG EnQiu4, WANG ShuChen4, XU HouGang4 & CHEN HongXing2 1 Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China 2 Beijing Institute of Biotechnology, Beijing 100071, China 3 China Agricultural University, Beijing 100193, China 4 Yutian Breeding Swine Farms, Yutian 064104, China | 2010 | Science China(Life Sciences)2010，53，4： | 21 |
| 8 | Synthesis of novel nanomaterials and their application in efficient removal of radionuclides显示文摘With the development of nuclear energy, large amounts of radionuclides are inevitably released into the natural environment. It is necessary to eliminate radionuclides from wastewater for the protection of environment. Nanomaterials have been considered as the potential candidates for the effective and selective removal of radionuclides from aqueous solutions under complicated conditions because of their high specific surface area, large amounts of binding sites, abundant functional groups, pore-size controllable and easily surface modification. This review mainly summarized the recent studies for the synthesis, fabrication and surface modification of novel nanomaterials and their applications in the efficient elimination and solidification of radionuclides,and discussed the interaction mechanisms from batch experiments, spectroscopy analysis and theoretical calculations. The sorption capacities with other materials, advantages and disadvantages of different nanomaterials are compared, and at last the perspective of the novel nanomaterials is summarized. | Xiangxue Wang Long Chen Lin Wang Qiaohui Fan Duoqiang Pan Jiaxing Li Fangting Chi Yi Xie Shujun Yu Chengliang Xiao Feng Luo Jun Wang Xiaolin Wang Changlun Chen Wangsuo Wu Weiqun Shi Shuao Wang Xiangke Wang | 2019 | Science China Chemistry2019，62，8： | 20 |
| 9 | Efects of a bioartificial liver support system on a cetaminophen-induced acute liver failure canines显示文摘Seeinvitedcommentaryonpage286Subjectheadingsliversupportsystem;acuteliverfailure;canines;porcinehepatocytes;bioartificiallive... | XUE Yi Long 1, ZHAO Shi Feng 2, ZHANG Zuo Yun 1, WANG Yue Feng 1, LI Xin Jian 1, HUANG Xiao Qiang 3, LUO Yun 1, HUANG Ying Cai 4 and LIU Cheng Gui 1 | 1999 | World Journal of Gastroenterology1999，5，4： | 19 |
| 10 | A new approach to consensus problems in discrete-time multiagent systems with time-delays显示文摘In this paper, consensus problems in discrete-time multiagent systems with time-invariant delays are considered. In order to characterize the structures of communication topologies, the concept of 'pre-leader-follower' decomposition is introduced. Then, a necessary and sufficient condition for state consensus is established. By this method, consensus problems in networks with a single time-delay, as well as with multiple time-delays, are studied, and some necessary and sufficient conditions for solvability of consensus problems are obtained. | WANG Long XIAO Feng | 2007 | Science in China(Series F)2007，50，4： | 19 |
| 11 | Effect of dietary supplementation with protease on growth performance,nutrient digestibility, intestinal morphology, digestive enzymes and gene expression of weaned piglets显示文摘This study was conducted to investigate the effect of dietary protease supplementation on the growth performance, nutrient digestibility, intestinal morphology, digestive enzymes and gene expression in weaned piglets. A total of 300 weaned piglets(21 days of age Duroc x Large White x Landrace; initial BW = 6.27 ± 0.45 kg) were randomly divided into 5 groups. The 5 diets were: 1) positive control diet(PC), 2) negative control diet(NC), and 3) protease supplementations, which were 100, 200, and 300 mg per kg NC diet. Results indicated that final BW, ADG, ADFI, crude protein digestibility, enzyme activities of stomach pepsin, pancreatic amylase and trypsin, plasma total protein, and intestinal villus height were higher for the PC diet and the supplementations of 200 and 300 mg protease per kg NC diet than for the NC diet(P < 0.05). Supplementations of 200 and 300 mg protease per kg NC diet significantly increased the ratio of villus height to crypt depth(VH:CD) of duodenum, jejunum and ileum compared with NC diet(P < 0.05). Feed to gain ratio, diarrhea index, blood urea nitrogen, and diamine oxidase were lower for the PC diet and supplementations of 200 and 300 mg protease per kg NC diet than for the NC diet(P < 0.05). Piglets fed the PC diet had a higher peptide transporter 1(PepTl) mRNA abundance in duodenum than piglets fed the NC diet(P < 0.05), and supplementations of 100, 200 and 300 mg protease per kg NC diet increased the PepTl mRNA abundance in duodenum(P < 0.05) comparing with the NC diet. Piglets fed the PC diet had a higher b0,+AT mRNA abundance in jejunum than piglets fed the NC diet(P < 0.05), and supplementations of 200 and 300 mg protease per kg NC diet increased the b0,+AT mRNA abundance in jejunum and ileum comparing with the NC diet(P < 0.05). In summary, dietary protease supplementation increases growth performance in weaned piglets, which may contribute to the improvement of intestinal development, protein digestibility, nutrient transport efficiency, and health status of piglets when fed low digestible protein sources. | Jianjun Zuo Baoming Ling Lina Long Tiejun Li Ludovic Lahaye Chengbo Yang Dingyuan Feng | 2015 | Animal Nutrition2015，，4： | 18 |
| 12 | Effect of multiple-phase regional intra-arterial infusion chemotherapy on patients with resectable pancreatic head adenocarcinoma显示文摘背景地区性的intra动脉的注入化疗( RIAC )是更珍贵的与不能实行的胰腺的腺癌改进病人的生活的预后和质量,并且辅助 RIAC 在延长幸存并且在胰腺的癌症的激进的切除术以后减少肝转移的风险起一个重要作用,但是外科手术前或多重阶段的 RIAC 的效果(外科手术前与手术后的 RIAC 结合了)为 resectable ,胰腺的癌症没被调查。在这未来的研究,为有 resectable 的病人的多重阶段的 RIAC 的效果胰腺的头腺癌被评估,并且它与手术后的 RIAC 作比较的安全和有效性也是有胰腺的头癌症随机被分到二个组的 resectable 的 assessed.Methods 病人。在组 A (n=50 ) 的病人与与多重阶段的 RIAC 相结合的扩大 pancreaticoduodenectomy 的新治疗学的模式被对待,并且那些在组 B (n=50 ) 被对待,扩大 pancreaticoduodenectomy 在一样的时期与手术后的 RIAC 结合了。新治疗学的模式的可行性,依从和效率被 RIAC 的肿瘤尺寸,浆液肿瘤标记,临床的利益反应(CBR ) ,外科的复杂并发症,死亡和毒性评估。没有疾病的幸存时间,中部的幸存时间,肝转移的发生,在 1, 2, 3 和 5 年的幸存率也被观察。生活曲线被在组 A 的疼痛消除率和 CBR 是的 Kaplan-Meier method.Results 产生 80% 和 84% 分别地。浆液肿瘤标记显然减少了,肿瘤尺寸在组 A 在外科手术前的 RIAC 以后在 26% 病人减少了。不再外科的复杂并发症,死亡或严重全身的副作用与组 B 相比在组 A 被观察。在组 A 的肝转移的发生是 34% 它在组 B 是比 50% 低的。在组 A 的没有疾病的幸存时间和中部的幸存时间分别地是 15.5 个月和 18 个月。1- , 2- , 3 年、 5 年的幸存率分别地是 54.87% , 34.94% , 24.51% 和 12.25% 。没有在多重阶段的 RIAC 是的二 groups.Conclusions 之间的幸存时间或幸存率的重要差别在 resectable 的联合治疗有效由提高肿瘤生长和肝转移的减小的抑制的胰腺的头癌,没有病人的安全或外科的过程上的否定效果。 | JIN Chen YAO Lie LONG Jiang FU De-liang YU Xian-jun XU Jin YANG Feng NI Quan-xing | 2009 | Chinese Medical Journal2009，，3： | 17 |
| 13 | The M5.0 Suining-Tongnan (China) earthquake of 31 January 2010:A destructive earthquake occurring in sedimentary cover显示文摘Usually, only crystalline basement is strong enough to store the massive strain energy that can be released in a damaging earthquake. By contrast, sedimentary cover is weak, because of its relatively high porosity and fluids. Therefore, it generally cannot accumulate enough energy for strong earthquakes. On January 31, 2010, a M5.0 earthquake occurred near the border of Suining and Tongnan in China. It excited strong short-period Rayleigh waves Rg, indicative of its shallow focal depth. The focal depth is constrained to less than 4 km, most probably in the range of 1-3 km, by modeling amplitude dependence on the frequency and waveforms of teleseismic depth phases (pP, sP). Because the local Mesozoic sedimentary cover is about 6 km thick, this earthquake should have occurred in the sedimentary cover. Though some shallow earthquakes with magnitudes up to M4 occur in Paleozoic sediments, this earthquake is the first M5 event studied in Mesozoic sedimentary cover. This event provides a rare opportunity to study seismogenic processes of damaging earthquakes in sedimentary basins. | LUO Yan NI SiDao ZENG XiangFang XIE Jun CHEN Yong LONG Feng | 2011 | Chinese Science Bulletin2011，56，6： | 17 |
| 14 | Geometry and tectonic deformation of the seismogenic structure for the 8 August 2017 M_S 7.0 Jiuzhaigou earthquake sequence,northern Sichuan, China显示文摘To reveal the geometry of the seismogenic structure of the Aug. 8, 2017 M_S 7.0 Jiuzhaigou earthquake in northern Sichuan,data from the regional seismic network from the time of the main event to Oct. 31, 2017 were used to relocate the earthquake sequence by the tomoDD program, and the focal mechanism solutions and centroid depths of the M_L ≥ 3.5 events in the sequence were determined using the CAP waveform inversion method. Further, the segmental tectonic deformation characteristics of the seismogenic faults were analyzed preliminarily by using strain rosettes and areal strains(As). The results indicate:(1) The relocated M_S 7.0 Jiuzhaigou earthquake sequence displays a narrow ~ 38 km long NNW-SSE-trending zone between the NW-striking Tazang Fault and the nearly NSstriking Minjiang Fault, two branches of the East Kunlun Fault Zone. The spatial distribution of the sequence is narrow and deep for the southern segment, and relatively wide and shallow for the northern segment. The initial rupture depth of the mainshock is 12.5 km, the dominant depth range of the aftershock sequence is between 0 and 10 km with an average depth of 6.7 km. The mainshock epicenter is located in the middle of the aftershock region, showing a bilateral rupture behavior. The centroid depths of 32 M_L ≥ 3.5 events range from 3 to 12 km with a mean of about 7.3 km, consistent with the predominant focal depth of the whole sequence.(2) The geometric structure of the seismogenic fault on the southern section of the aftershock area(south of the mainshock) is relatively simple, with overall strike of ~150° and dip angle ~75°, but the dip angle and dip-orientation exhibit some variation along the segment. The seismogenic structure on the northern segment is more complicated; several faults, including the Minjiang Fault, may be responsible for the aftershock activities. The overall strike of this section is ~159° and dip angle is ~59°, illustrating a certain clockwise rotation and a smaller dip angle than the southern segment. The differences between the two segments demonstrate variation of the geometric structure along the seismogenic faults.(3) The focal mechanism solutions of 32 M_L ≥ 3.5 events in the earthquake sequence have obvious segmental characteristics. Strike-slip earthquakes are dominant on the southern segment, while 50% of events on the northern segment are thrusting and oblique thrusting earthquakes, revealing significant differences in the kinematic features of the seismogenic faults between the two segments.(4) The strain rosettes for the mainshock and the entire sequence of 31 M_L ≥ 3.5 aftershocks correspond to strike-slip type with NWW-SEE compressional white lobes and NNE-SSW extensional black lobes of nearly similar size. The strain rosette and As value of the entire sequence of 22 M_L ≥ 3.5 events on the southern segment are the same as those of the M_S 7.0 mainshock,indicating that the tectonic deformation here is strike-slip. However, the strain rosette of the entire sequence of 10 M_L ≥ 3.5 events on the northern segment show prominent white compressional lobes and small black extensional lobes, and the related As value is up to 0.52,indicating that the tectonic deformation of this segment is oblique thrusting with a certain strike-slip component. Differences between the two segments all reveal distinctly obvious segmental characteristics of the tectonic deformation of the seismogenic faults for the Jiuzhaigou earthquake sequence. | Feng Long GuiXi Yi SiWei Wang YuPing Qi Min Zhao | 2019 | Earth and Planetary Physics2019，3，3： | 17 |
| 15 | Leakage Prediction Method for Contacting Mechanical Seals with Parallel Faces显示文摘Since the beginning of the 20th century, many researches on the sealing characteristic of mechanical seals were carried out broadly and in depth by various methods and some leakage models were built. But due to the lack of the way to characterize the main factors of influence on the leakage, most of the early researches were based on the assumptions that the seal faces topography and the frictional conditions were invariant. In the early built models, the effect of the surface topography change of the seal face on the leakage rate was neglected. Based on the fractal theory, the contact of end faces of the rotary and stationary rings was simplified to be the contact of a rough surface and an ideal rigid smooth surface, and the contact interface’s cavity size-distribution function as well as the fractal characteristic of the cavity profile curve was discussed. By analyzing the influence of abrasion on the seal face topography and the leakage channel, the time-correlation leakage prediction model of mechanical seals based on the fractal theory was established and the method for predicting the leakage rate of mechanical seals with parallel plane was proposed. The values of the leakage rate predicted theoretically are similar to the measured values of the leakage rate in the model test and in situ test. The experimental results indicate that the leakage rate of mechanical seals is a transient value. The surface topography of the end faces of the seal rings and its change during the frictional wear of mechanical seals can be accurately characterized by the fractal parameters. Under the work conditions of changeless frictional mechanism, the fractal parameters measured or calculated based on the accelerated testing equation can be used to predict the leakage rate of mechanical seal in service. The proposed research provides the basis for determining the leakage state and predicting working life of mechanical seal. | SUN Jianjun WEI Long FENG Xiu GU Boqin | 2010 | Chinese Journal of Mechanical Engineering2010，23，1： | 16 |
| 16 | Cathepsin L upregulation-induced EMT phenotype is associated with the acquisition of cisplatin or paclitaxel resistance in A549 cells显示文摘组织蛋白酶 L (CTSL ) , lysosomal 酸半胱氨酸朊酶,被知道在肿瘤起重要作用转移和化疗抵抗。在这研究,我们调查了在人的肺癌症 cells.Methods 由 CTSL 位于 chemoresistance 的规定下面的分子的机制:人的肺癌症 A549 房间, A549/PTX (paclitaxel 抵抗) 房间和 A549/DDP (cisplatin 抵抗) 房间被测试。到 cisplatin 或 paclitaxel 的抵抗用 MTT 和殖民地形成试金被检测。肌动朊改变与 FITC-Phalloidin 被观察荧光灯的染色或 immunofluorescence。愈合创伤的试金或 Transwell 试金被用来估计迁居或侵略能力。CTSL 和上皮、间充质的标记的表示与西方的弄污和 immunofluorescence 被分析。联系 EMT 的抄写因素的表示与西方的弄污或 q-PCR 被测量。BALB/c 裸体老鼠与 A549 房间 overexpressing CTSL 皮下地被植入,并且老鼠是管理 paclitaxel (10, 15 mg/kg, ip ) 为 5 times.Results 的每 3 d:Cisplatin 或 paclitaxel 治疗(10-80 ng/mL ) 在 A549 房间导致了 CTSL 表示。CTSL 层次比在 A549 房间在 A549/PTX 和 A549/DDP 房间是高得多的。CTSL 的 Silencing 在 A549/DDP 和 A549/TAX 房间颠倒了 chemoresistance,而 CTSL 的 overexpression 稀释了 A549 房间的敏感到 cisplatin 或 paclitaxel。而且, A549/DDP 和 A549/TAX 房间经历了词法并且有增加的房间侵略和迁居能力的细胞骨架变化,由上皮的标记( E-cadherin 和 cytokeratin-18 )的减少的表示伴随了并且增加间充质的标记( N-cadherin 和 vimentin )的表示,以及 联系EMT 的抄写因素的 upregulation 蜗牛,蛞蝓, ZEB1 和 ZEB2 。CTSL 的 Silencing 在 A549/DDP 和 A549/TAX 房间颠倒了 EMT;相反, CTSL 的 overexpression 在 A549 房间导致了 EMT。在异种皮移植裸体人鼠标模型,与 A549 房间 overexpressing CTSL 植入的鼠标展出了显著地减少的敏感到 paclitaxel 处理,并且在肿瘤 tissues.Conclusion 增加了联系 EMT 的蛋白质和抄写因素的表示:Cisplatin 和 paclitaxel 抵抗在 A549 房间与 CTSL 导致 upregulation 的 EMT 被联系。因此,调停 CTSL 的 EMT 可以作为一个目标被利用对肺癌症和恶意的另外的类型提高 cisplatin 或 paclitaxel 的功效。 | Mei-ling HAN Yi-fa n ZHAO Cai-hong TAN Ya-jie XIONG Wen-juan WANG Feng WU Yao FEI Long WANG Zhong-qin LIANG | 2016 | Acta Pharmacologica Sinica2016，37，12： | 15 |
| 17 | Xiaotan Sanjie decoction attenuates tumor angiogenesis by manipulating Notch-1-regulated proliferation of gastric cancer stem-like cells显示文摘AIM: To determine the underlying mechanisms of action and influence of Xiaotan Sanjie(XTSJ) decoction on gastric cancer stem-like cells(GCSCs). METHODS: The gastric cancer cell line MKN-45 line was selected and sorted by FACS using the cancer stem cell marker CD44; the stemness of these cells was checked in our previous study. In an in vitro study, the expression of Notch-1, Hes1, Vascular endothelial growth factor(VEGF), and Ki-67 in both CD44-positive gastric cancer stem-like cells(GCSCs) and CD44-negative cells was measured by Western blot. The effect of XTSJ serum on cell viability and on the above markers was measured by MTT assay and Western blot, respectively. In an in vivo study, the ability to induce angiogenesis and maintenance of GCSCs in CD44-positive-MKN-45- and CD44-negative-engrafted mice were detected by immunohistochemical staining using markers for CD34 and CD44, respectively. The role of XTSJ decoction in regulating the expression of Notch-1, Hes1, VEGF and Ki-67 was measured by Western blot and real-time polymerase chain reaction.RESULTS: CD44+ GCSCs showed more cell proliferation and VEGF secretion than CD44-negative cells in vitro, which were accompanied by the high expression of Notch-1 and Hes1 and positively associated with tumor growth(GCSCs vs CD44-negative cells, 2.72 ± 0.25 vs 1.46 ± 0.16, P < 0.05) and microvessel density(MVD)(GCSCs vs CD44-negative cells, 8.15 ± 0.42 vs 3.83 ± 0.49, P < 0.001) in vivo. XTSJ decoction inhibited the viability of both cell types in a dose-dependent manner in vitro. Specifically, a significant difference in the medium-(82.87% ± 6.53%) and high-dose XTSJ groups(77.43% ± 7.34%) was detected at 24 h in the CD44+ GCSCs group compared with the saline group(95.42% ± 5.76%) and the low-dose XTSJ group(90.74% ± 6.57%)(P < 0.05). However, the efficacy of XTSJ decoction was reduced in the CD44- groups; significant differences were only detected in the high-dose XTSJ group at 48 h(78.57% ± 6.94%) and 72 h(72.12% ± 7.68%) when compared with the other CD44- groups(P < 0.05). Notably, these differences were highly consistent with the Notch-1, Hes1, VEGF and Ki-67 expression in these cells. Similarly, in vivo, XTSJ decoction inhibited tumor growth in a dose-dependent manner. A significant difference was observed in the medium(1.76 ± 0.15) and high-dose XTSJ(1.33 ± 0.081) groups compared with the GCSCs control group(2.72 ± 0.25) and the low-dose XTSJ group(2.51 ± 0.25)(P < 0.05). We also detected a remarkable decrease of MVD in the medium-(7.10 ± 0.60) and high-dose XTSJ(5.99 ± 0.47) groups compared with the GCSC control group(8.15 ± 0.42) and the low-dose XTSJ group(8.14 ± 0.46)(P < 0.05). Additionally, CD44 expression was decreased in these groups [medium-(4.43 ± 0.45) and high-dose XTSJ groups(3.56 ± 0.31) vs the GCSC control(5.96 ± 0.46) and low dose XTSJ groups(5.91 ± 0.38)](P < 0.05). The significant differences in Notch-1, Hes1, VEGF and Ki-67 expression highly mirrored the results of XTSJ decoction in inhibiting tumor growth, MVD and CD44 expression.CONCLUSION: Notch-1 may play an important role in regulating the proliferation of GCSCs; XTSJ decoction could attenuate tumor angiogenesis, at least partially, by inhibiting Notch-1. | Bing Yan Long Liu Ying Zhao Li-Juan Xiu Da-Zhi Sun Xuan Liu Ye Lu Jun Shi Yin-Cheng Zhang Yong-Jin Li Xiao-Wei Wang Yu-Qi Zhou Shou-Han Feng Can Lv Pin-Kang Wei Zhi-Feng Qin | 2014 | World Journal of Gastroenterology2014，20，36： | 14 |
| 18 | Effect of endotoxin on fibronectin synthesis of rat primary cultured hepatocytes显示文摘EfectofendotoxinonfibronectinsynthesisofratprimaryculturedhepatocytesJIAJinBin,HANDeWu,XURuiLing,GAOFei,ZHAOLongFeng,ZHA... | JIA JinBin, HAN DeWu, XU RuiLing, GAO Fei, ZHAO LongFeng, ZHAO YuanChang, YAN JinPin and MA XueHuiKeywords liver cirrhosis endotoxins fibronectin/analysis liver/cytology cells, cultured lipopolysaccharide | 1998 | World Journal of Gastroenterology1998，4，4： | 14 |
| 19 | Object Detection Research of SAR Image Using Improved Faster Region-Based Convolutional Neural Network显示文摘Target detection technology of synthetic aperture radar(SAR)imageis widely used in the field of military reconnaissance and surveillance.The traditional SAR image target detection methods need to be provided a lot of empirical knowledge because the characteristics of SAR images in different configurations(attitude,pitch angle,imaging parameters,etc.)will change greatly,resulting in high generalization error.Currently,deep learning method has achieved great success in the field of image processing.Research shows that deep learning can achieve a more intrinsic description of the data,while the model has a stronger ability of modeling and generalization.In order to solve the problem of insufficient data in SAR data sets,an experimental system for acquiring SAR image data in real scenes was built.Then the transfer learning method and the improved convolution neural network algorithm(PCA+Faster R-CNN)are applied to improve the target detection precision.Finally,experimental results demonstrate the significant effectiveness of the proposed method. | Long SUN Tao WU Guangcai SUN Dazheng FENG Lieshu TONG Mengdao XING | 2020 | Journal of Geodesy and Geoinformation Science2020，3，3： | 13 |
| 20 | Three dimensional velocity structure and accurate earthquake location in Changning–Gongxian area of southeast Sichuan显示文摘In order to understand the crustal structure and tectonic background of the Changning–Gongxiang area, southeastern Sichuan Province, where a series of moderate-to-strong earthquakes occurred in recent years, we utilized the seismic phase data both from a local dense array and from the regional seismic networks;we used the tomoDD program to invert for the high-resolution three-dimensional velocity structure within the depth range of 0–10 km and for accurate hypocentral locations in this area. We analyzed the seismogenic structures for the events of Xingwen M5.7 in 2018 and Gongxian M5.3 and Changning M6.0 in 2019. The results show that:(1) widespread lateral inhomogeneity exists in the velocity structure of the study area, and the location of the velocity anomaly is largely consistent with known structures. In the range of distinguishable depth, the inhomogeneity decreases with increasing depth, and the velocity structure anomalies in some areas are continuous in depth;(2) earthquakes occurred in clusters, showing the characteristics of zonal folding trends in the NW-SE and NE-SW directions;the focal depth in the area is generally shallow in both the sedimentary cap and the crystalline basement. The seismogenic structures of small earthquake clusters are different in size and occurrence in different sections, and the clusters occurred mostly in regions with high P-or S-wave velocities;(3) synthesis of a variety of data suggests that the seismogenic structures of the Xingwen M5.7 and Changning M6.0 earthquakes are associated with slip faults that trend NW-SE in, respectively, the south wing and the axis of the Changning–Shuanghe anticline, while that of the Gongxian M5.3 earthquake is associated with thrust faults that trend N-S in the Jianwu syncline region. The dynamic sources of the three earthquakes are all from the SE pushing of the Qinghai–Tibet block on the Sichuan basin;(4) the risk of future strong earthquakes in this area must be reevaluated in light of the facts(a)that in recent years, moderate-to-strong earthquake swarms have occurred frequently in southeast Sichuan;(b) that the complex structural area exhibits the easy-to-trigger characteristic, and(c) that the small-scale faults in this area are characterized by the phenomenon of stress 'lock and release'. | Feng Long ZhiWei Zhang YuPing Qi MingJian Liang Xiang Ruan WeiWei Wu GuoMao Jiang LongQuan Zhou | 2020 | Earth and Planetary Physics2020，4，2： | 13 |
您所在的机构暂未开通此部分的功能，请联系管理员