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| 1 | Isolation and characterization of potential antibiotic producing actinomycetes from water and sediments of Lake Tana,Ethiopia显示文摘Objective:To isolate,evaluate and characterize potential antibiotic producing actinomycetes from water and sediments of Lake Tana,Ethiopia.Methods:A total of 31 strains of actinomycetes were isolated and tested against Gram positive and Gram negative bacterial strains by primary screening.In the primary screening.11 promising isolates were identified and subjected to solid state and submerged state fermentation methods to produce crude extracts.The fermented biomass was extracted by organic solvent extraction method and tested against bacterial strains by disc and agar well diffusion methods.The isolates were characterized by using morphological,physiological and biochemical methods.Results:The result obtained from agar well diffusion method was belter than disc diffusion method.The crude extract showed higher inhibition zone against Gram positive bacteria than Cram negative bacteria.One-way analysis of variance confirmed most of the crude extracts were statistically significant at 95%confidence interval.The minimum inhibitor)concentration and minimum bactericidal concentration of crude extracts were 1.65 mg/mL and 3.30 mg/mL against Staphylococcus aureus,and 1.84 mg/mL and 3.80 mg/mL against Escherichia coli respectively.The growth of aerial and substrate mycelium varied in different culture media used.Most of the isolates were able to hydrolysis starch and urea:able to survive at 5%concentration of sodium chloride:optimum temperature for their growth was 30°C.Conclusions:The results of the present study revealed that freshwater actinomycetes of Luke Tana appear to have immense potential as a source ol antibacterial compounds. | Gebreselema Gebreyohannes Feleke Moges Samuel Sahil Nagappan Raja | 2013 | Asian Pacific Journal of Tropical Biomedicine2013，3，6： | 12 |
| 2 | In vitro antibacterial activity of leaf extracts of Zehneria scabra and Ricinus communis against Escherichia coli and methicillin resistance Staphylococcus aureus显示文摘Objective:To evaluate the antibacterial activities of the crude leaves extracts of Zehneria scabra(Z.scabra) and Ricinus communis(R.communis) against Escherichia coli(E.coli).Staphylococcus aureus(S.aureus) and methicillin resistance 5.aureus.Methods:The crude powdered leaves of Z.scabra and R.communis were extracted successively by organic solvents in increasing polarity[benzene,chloroform:acetone(1:1),70%alcohol and distilled water].The antibacterial susceptibility of the crude leaves extracts of were tested against standard strains of E.coli(ATCC 25922) and S.aureus(ATCC 2923) and clinical isolates of E.coli,S.aureus and methicillin resistance 5.aureus using agar well diffusion method.Results:In Z.scabra and R.communis leaf extracts,the most sensitive standard strain was S.aureus with an inhibition zone of(14.00±1.20) mm and(15.90±2.13) mm,respectively.The minimum inhibitory concentration(MIC) values of Z.scabra extracts against test organisms ranged from 1.95mg/mL for extract 3 in clinical and standard strains of 5.aureus to 250 mg/mL for extract 1 and 4in clinical and standard strains of E.coli.The MIC values of R.communis extracts against test organisms ranged from 1.95 mg/mL for extract 2 and 3 standard strains of S.aureus to 250 mg/mL for extract 1 in clinical isolate of E.coli.Most of the minimum bactericidal concentration and MIC values of plant extracts were almost similar particularly in R.communis,or minimum bactericidal concentration equal to one dilution factor less than MIC value of the extracts mainly in Z.scabra.Conclusions:The potency of plant extracts against test organisms were depend on different organic solvents used.Clinical isolate of bacterial pathogens showed less zones of diameter compared to the standard strains.Gram-positive had wide inhibition zones than Gram-negative bacteria.Further studies should be carried out to isolate the pure compounds and standardization of the methods of plant extracts for an in vitro testing. | Bereket Abew Samuel Sahile Feleke Moges | 2014 | Asian Pacific Journal of Tropical Biomedicine2014，4，10： | 3 |
| 3 | Seroprevalence of Helicobacter pylori in dyspeptic patients and its relationship with HIV infection,ABO blood groups and life style in a university hospital,Northwest Ethiopia显示文摘瞄准:在消化不良的病人之中决定 Helicobacter pylori (H pylori ) 的流行并且估计在 H pylori 感染,血型, HIV 感染和病人的生活方式之间的关系。方法:在基于医院的代表性的研究, Gondar 医院的大学的病人出席门诊病人部门被注册。Socio 人口统计的信息用问询表被收集。浆液用一个商业工具包为 anti-H pylori IgG 抗体被分析。HIV serostatus 被连接酶的免疫吸着剂试金(ELISA ) 决定。Blood grouping 被幻灯片凝集试验执行。结果:215 个消化不良的病人的一个总数在学习被包括。116 个病人(54%) 是女性,(46%) 99 是男性。Anti-H pylori IgG 抗体在重量的单位被检测一 of 184 (85.6%) 病人。流行在 50 年并且在上面的病人是显著地更高的。百分之五个病人被发现是为 HIV 的血清反应阳性的二十个点。没有重要协会为 H pylori 在性别,土佬血型,香的食谱的消费,社会经济的地位和 seropositivity 之间被发现。然而,白酒消费显著地与 H pylori 血清学被联系。结论:H pylori 感染的流行与白酒吸入和老年的历史被联系。象为 H pylori 感染的风险因素的不同饮食,酒精和社会经济的地位的效果需要进一步的学习。 | Feleke Moges Afework Kassu Getahun Mengistu Solomon Adugna Berhanu Andualem Takeshi Nishikawa Fusao Ota | 2006 | World Journal of Gastroenterology2006，12，12： | 2 |
| 4 | Isolation and screening of antibiotic producing actinomycetes from soils in Gondar town, North West Ethiopia显示文摘 | Abebe Bizuye Feleke Moges Berhanu Andualem | 2013 | Asian Pacific Journal of Tropical Disease2013，，5： | 2 |
| 5 | Emotional distress before coronary bypass grafting limits the benefits of surgery显示文摘 | Perski A Feleke E Anderson G | | 0，，： | 1 |
| 6 | Selective reaction of nitrate to nitrogen gas in a biofilm-electrode reactor显示文摘 | Z feleke K araki Y sakakaibara | 1998 | Wat Res1998，32，9： | 1 |
| 7 | Prevalence and clinical features of tuberculosis in Ethiopian diabetic patients 显示文摘 | Feleke Y Abdulkadir J Aderaye G | 1999 | East African Med J1999，76，： | 1 |
| 8 | A case presentation of bilateral simultaneous Bell' s palsy显示文摘 | Kilic R OzdekA Felek S | 2003 | Am J Otolaryngol2003，24，2： | 1 |
| 9 | Selective reduc-tion of nitrate to nitrogen gas in a biofilm-electrode re- actor显示文摘 | Feleke Z Araki K Sakakibara Y | 1998 | Water Research1998，32，9： | 1 |
| 10 | Anticancer activity of VDR- coregulator inhibitor PS 121912显示文摘 | Sidhu PS Teske K Feleke B | 2014 | Cancer Chemother Pharmacol2014，74，4： | 1 |
| 11 | Prevalence and clinical features of tuberculosis in Ethiopian diabetic patients 显示文摘 | Feleke Y Abdulkadir J Aderaye G | 1999 | East African Med J1999，76，： | 1 |
| 12 | Smoking, HIV and non-fatal tuberculosis in an urban African population显示文摘 | Ramin B Kam D Feleke B Jacob B Jha P | 2008 | Int Tuber Lung Dis2008，12，6： | 1 |
| 13 | Benefits of low dose immunoglobtdin in the treatment of refractory CAPD peritonitis and longevity of technical survival on CAPD显示文摘 | Dursun B Tuncer M Felek R | 2005 | Int Urol Nephrol2005，37，10： | 1 |
| 14 | Prediction of future uniform milk prices in Florida federal milk marketing order 6 from milk futures market显示文摘 | De Vries A Feleke S | | 0，，91： | 1 |
| 15 | Diabetic infections:clinical and bacteriological study at Tikur Anbessa Specialized University hospital,Addis Ababa,Ethiopia显示文摘 | Feleke Y Mengistu Y Enquselassie F | 2007 | Ethiop Med J2007，45，2： | 1 |
| 16 | A bio-electrochemical reactor coupled with adsorber for the removal of nitrate and inhibitory pesticide显示文摘 | Z Feleke Y Sakakibara | 2002 | Water Research2002，36，： | 1 |
| 17 | Selective reduction of nitrate to nitrogen gas in a biofilm-electrode reactor 显示文摘 | FELEKE Z ARAKI K SAKAKIBARA Y | 1998 | Water Res1998，32，9： | 1 |
| 18 | Coupled consolidation and contaminant transport model for simulating migration of contaminants through the sediment and a cap 显示文摘 | Feleke Arega Earl Hayte | 2008 | Applied Mathematical Modelling2008，32，11： | 1 |
| 19 | Diabetic infections:clinical and bacteriological study at Tikur Anbessa Specialized University Hospita,Addis Ababa,Ethiopia显示文摘 | Feleke Y Mengistu Y Enquselassie F | 2007 | Ethiop Med J2007，45，2： | 1 |
| 20 | A bio-electrochemical reactor coupled with adsorber for the removal of nitrate and inhibitory pesticide显示文摘 | Feleke Z Sakakibara Y | 2002 | Water Research2002，36，12： | 1 |
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