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| 1 | Immunocytochemical identification and localization of APUD cells in the gut of seven stomachless teleost fishes显示文摘AIM To study the cell types,localization,distribution density and morphology of APUDcells in the intestinal mucosa of stomachlessteleost fishes.METHOD By using the peroxidase-antiperoxidase complex(PAP)immunocytochemical staining technique theidentification,localization and morphology ofimmunoreactive(IR)endocrine cells seattered inthe intestinal mucosa of grass carp(Cyenopharyngodon idellus),black carp(Mylopharyngodon piceus)and common carp(Cyprinus carpio)were investigated with 20kinds of antisera prepared against mammalianpeptide hormones of APUD cells,and likewise byusing avidin-biotin-peroxidase complex(ABC)method those of silver carp(Hypophthalmichthys molitrix),bighead(Aristichthys nobilis),silver crucian carp(Carassius gibelio)and bluntnose black bream(Megalobrama amblyocephala)were alsostudied with 5 different antisera.Thereplacement of the first antiserum by phosphatebuffered saline(PBS)was employed as a control.IR endocrine cells were counted with asquare-mesh ocular micrometer from 10 fieldsselected randomly in every section of each partof the intestine specimen.The average numberof IR endocrine cells per mm2 was counted toquantify their distribution density.RESULT Gastrin(GAS)-,Gastric inhibitorypeptide(GIP)-,glucagon(GLU)-,glucagon-likeimmunoreactants(GLI)-,bovine pancreaticpolypeptide(BPP)-,leucine-enkephalin(ENK)-and substance P(SP)-IR endocrine cells werefound in the gut of grass carp,black carp andcommon carp,and somatostatin(SOM)-IRendocrine cells were only seen in common carp.GAS-,GIP-and GLU-IR endocrine cells werefound in the intestinal mucosa of silver carp,bighead,silver crucian carp and bluntnose blackbream.Most of IR endocrine cells had the higherdistribution density in the foregut and midgut,and were longer in shape.They had a long apicalcytoplasmic process extended to the gut lumenand a basal process extended to adjacent cellsor basement membrane and touched with it.Sometimes,the basal cytoplasmic processformed an enlarged synapse-like structure in thecontiguous part with basement membrane.Thisphenomenon provided new morphologicalevidence for neuroendocrine and paracrinesecretory function of these enteroendocrinecells.CONCLUTION At least 8 kinds of IR endocrinecells were found in the gut of stomachlessteleost species for the first time in China.TheseIR endocrine cells scattering in the gut mucosabelong to the APUD system.Among them,thehormones secreted by SP-,ENK-,SOM-and GLU-IR endocrine cells belong to the peptides of dualdistribution in the brain and gut.This providednew evidence for the concept of brain-gutpeptide.According to the cell types,distribution density,morphologicalcharacteristics and variety in shape of APUDcells in the gut of stomachless teleost fishes,itis deemed that the digestive tract of fishes isalso an endocrine organ of great importance andcomplexity. | Pan QS Fang ZP Zhao YX | 2000 | World Journal of Gastroenterology2000，6，1： | 25 |
| 2 | HERG K^+ channel blockade by the novel antiviral drug sophocarpine 显示文摘 | ZHAO XL QI ZP FANG C | 2008 | Biol Pharm Bull2008，31，6： | 1 |
| 3 | SirT1 mediates hyperbaric oxygen preconditioning-induced ischemic tolerance in rat brain 显示文摘 | Yan WJ Fang ZP Yang QZ | 2013 | J Cereb Blood Flow Metab2013，33，3： | 1 |
| 4 | Novel method for the synthesis of 3 - monosubstituted six - membered benzosultams 显示文摘 | Fang F Wang R Liu ZP | 2007 | Heterocy- cles2007，71，11： | 1 |
| 5 | A new method to perpare chitosan membrance as a biomedical material 显示文摘 | Hu QL Fang ZP Zhao Y | 2001 | Chinese J Poly - Mer Science2001，19，5： | 1 |
| 6 | HGFK1 inhibits bone metastasis in breast cancer through the TAK1/p38 MAPK signaling pathway显示文摘 | Yao Y Fang ZP Chen H | 2012 | Cancer Gene Ther2012，19，9： | 1 |
| 7 | SirT1 mediates hyper- baric oxygen preconditioning-induced ischemic tolerance in rat brain显示文摘 | Yan WJ Fang ZP Yang QZ | 2013 | J Cereb Blood Flow Metab2013，33，3： | 1 |
| 8 | Pharmacokinetics,pharmacodynamics,metabolism,distribution,and excretion of carfilzomib in rats显示文摘 | Yang JF Wang ZP Fang Y | 2011 | Drug Metab Dispos2011，39，10： | 1 |
| 9 | Selective activation of small motor ax-ons by quasitrapezoidal current pulse显示文摘 | Fang ZP Mortimer JT | 1991 | IEEE Trans Biomed Eng1991，38，2： | 1 |
| 10 | Note: real time three -dimen-sional topography measurement of microfluidic deviceswith pillar structures using confocal microscope 显示文摘 | Ang KT Fang ZP Tay A | 2014 | Re-view of Scientific Instruments2014，85，2： | 1 |
| 11 | Polymorphism of nylon-6 in multi- walled carbon nanotube/nylon-6 composites显示文摘 | Li J Fang ZP Tong LF | 2006 | Journal of Polymer Sci- ence2006，44，10： | 1 |
| 12 | Toughening of cyanate ester resin by carboxyl terminated nitrile rubber 显示文摘 | Feng Y Fang ZP Gu AJ | 2004 | Polymers for Advanced Technologies2004，15，10： | 1 |
| 13 | Carboxyl - terminated butadiene - acrylonitrile rubber modified cyanate ester resin 显示文摘 | Shi HH Fang ZP | 2007 | Journal of Applied Polymer Science2007，106，： | 1 |
| 14 | Synthesis of 99mTc- TRODAT-1 as dopamine transporter imaging agent 显示文摘 | CHEN ZP ZHOU X | 2000 | Chin J Pharm ( in Chinese )2000，31，6： | 1 |
| 15 | Melatonin ameliorates vascular endothelial dysfunction,inflammation,andatherosclerosis by suppressing the TLR4/NF-κB system in high-fat-fed rabbits显示文摘 | Hu ZP Fang XL Fang N | 2013 | J Pineal Res2013，55，4： | 1 |
| 16 | Setective activation of small motor axons by quasi-trapezoidal current pulses 显示文摘 | Fang ZP Mortimer IT | 1991 | IEEE Trans Biomed Eng1991，38，2： | 1 |
| 17 | A novel intumescent flame retardant:Synthesis and application in ABS copolymer显示文摘 | Ma HY Tong LF Xu ZB Fang ZP Jin YM Lu FZ | | 0，，： | 1 |
| 18 | A subjective and objective integrated approach to determine attribute weights 显示文摘 | Fang ZP Huang LH | 1999 | European Journal of Operational research1999，112，： | 1 |
| 19 | Toughening of Polystyrene with Ethylene - Propylene- Diene Terpolymer (EPDM) Compatibilized by Styrene - Butadiene -Styrene Block Copolymer (SBS) 显示文摘 | Fang ZP Guo ZH Zha LL | 2004 | Macromolecular Materials and Engineering2004，289，8： | 1 |
| 20 | Melatonin ameliorates vascuiar endo- thelial dysfunction, inflammation, and atheroselerosis by suppressing the TLR4/NF-KB system in high-fat- fed rabbits显示文摘 | Hu ZP Fang XL Fang N | 2013 | J Pineal Res2013，55，4： | 1 |
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