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| 1 | Large-field high-resolution two-photon digital scanned light-sheet microscopy显示文摘 | Weijian Zong Jia Zhao Xuanyang Chen Yuan Lin Huixia Ren Yunfeng Zhang Ming Fan Zhuan Zhou Heping Cheng Yujie Sun Liangyi Chen | 2015 | Cell Research2015，25，2： | 12 |
| 2 | Scalable volumetric imaging for ultrahigh-speed brain mapping at synaptic resolution显示文摘The speed of high-resolution optical imaging has been a rate-limiting factor for meso-scale mapping of brain structures and functional circuits,which is of fundamental importance for neuroscience research.Here,we describe a new microscopy method of Volumetric Imaging with Synchronized on-the-fly-scan and Readout(VISo R)for high-throughput,high-quality brain mapping.Combining synchronized scanning beam illumination and oblique imaging over cleared tissue sections in smooth motion,the VISo R system effectively eliminates motion blur to obtain undistorted images.By continuously imaging moving samples without stopping,the system achieves high-speed 3D image acquisition of an entire mouse brain within1.5 hours,at a resolution capable of visualizing synaptic spines.A pipeline is developed for sample preparation,imaging,3D image reconstruction and quantification.Our approach is compatible with immunofluorescence methods,enabling flexible cell-type specific brain mapping and is readily scalable for large biological samples such as primate brains.Using this system,we examined behaviorally relevant whole-brain neuronal activation in 16 c-Fos-sh EGFP mice under resting or forced swimming conditions.Our results indicate the involvement of multiple subcortical areas in stress response.Intriguingly,neuronal activation in these areas exhibits striking individual variability among different animals,suggesting the necessity of sufficient cohort size for such studies. | Hao Wang Qingyuan Zhu Lufeng Ding Yan Shen Chao-Yu Yang Fang Xu Chang Shu Yujie Guo Zhiwei Xiong Qinghong Shan Fan Jia Peng Su Qian-Ru Yang Bing Li Yuxiao Cheng Xiaobin He Xi Chen Feng Wu Jiang-Ning Zhou Fuqiang Xu Hua Han Pak-Ming Lau Guo-Qiang Bi | 2019 | National Science Review2019，6，5： | 7 |
| 3 | Genomic insights into the fast growth of paulownias and the formation of Paulownia witches' broom显示文摘Paulownias are among the fastest growing trees in the world,but they often suffer tremendous loss of wood production due to infection by Paulownia witches'broom(PaWB)phytoplasmas.In this study,we have sequenced and assembled a high-quality nuclear genome of Paulownia fortunei,a commonly cultivated paulownia species.The assembled genome of P.fortunei is 511.6 Mb in size,with 93.2%of its sequences anchored to 20 pseudo-chromosomes,and it contains 31985 protein-coding genes.Phylogenomic analyses show that the family Paulowniaceae is sister to a clade composed of Phrymaceae and Orobanchaceae.Higher photosynthetic efficiency is achieved by integrating C3 photosynthesis and the crassulacean acid metabolism pathway,which may contribute to the extremely fast growth habit of paulownia trees.Comparative transcriptome analyses reveal modules related to cambial growth and development,photosynthesis,and defense responses.Additional genome sequencing of PaWB phytoplasma,combined with functional analyses,indicates that the effector PaWB-SAP54 interacts directly with Paulownia PfSPLa,which in turn causes the degradation of PfSPLa by the ubiquitin-mediated pathway and leads to the formation of witches'broom.Taken together,these results provide significant insights into the biology of paulownias and the regulatory mechanism for the formation of PaWB. | Yabing Cao Guiling Sun Xiaoqiao Zhai Pingluo Xu Liming Ma Minjie Deng Zhenli Zhao Haibo Yang Yanpeng Dong Zhonghai Shang Yujie Lv Lijun Yan Haifang Liu Xibing Cao Bingbing Li Zhe Wang Xiaogai Zhao Haiyan Yu Fan Wang Wen Ma Jinling Huang Guoqiang Fan | 2021 | Molecular Plant2021，14，10： | 7 |
| 4 | Water softening by induced crystallization in fluidized bed显示文摘Fluidized bed and induced crystallization technology were combined to design a new type of induced crystallization fluidized bed reactor. The added particulate matter served as crystal nucleus to induce crystallization so that the insoluble material, which was in a saturated state, could precipitate on its surface. In this study, by filling the fluidized bed with quartz sand and by adjusting water p H, precipitation of calcium carbonate was induced on the surface of quartz sand, and the removal of water hardness was achieved.With a reactor influent flow of 60 L/hr, a fixed-bed height of 0.5 m, pH value of 9.5, quartz sand nuclear diameter of 0.2–0.4 mm, and a reflux ratio of 60%, the effluent concentration of calcium hardness was reduced to 60 mg/L and 86.6% removal efficiency was achieved. The resulting effluent reached the quality standard set for circulating cooling water. Majority of the material on the surface of quartz sand was calculated to be calcium carbonate based on energy spectrum analysis and moisture content was around 15.994%. With the low moisture content, dewatering treatment is no longer required and this results to cost savings on total water treatment process. | Yuefang Che Rong Fan Danfeng An Yujie Cheng Hazel Tan | 2016 | Journal of Environmental Sciences2016，28，12： | 6 |
| 5 | Kinetic investigation of in situ growth of CdMoO_4 nano-octahedra显示文摘CdMoO4 nano-octahedra were grown in situ at room temperature by reverse-microemulsion.Energy evolution from this growth process was monitored using microcalorimetry.The microcalorimetric heat flow (MCHF) curve showed a characteristic endothermic peak for the initial reaction,and double discontinuous exothermic peaks for the subsequent crystal growth.Combined with complementary characterization techniques,the evolution of morphology and size of the CdMoO4 nano-octahedra were correlated with the MCHF peaks.Calculations based on the microcalorimetric results at 298.15 K provided rate constants of 7.56×10–5 s–1 for the reaction and nucleation process and 1.59×10–4 s–1 for the crystallization process. | LI YanFen JIANG JunYing FAN GaoChao MA YuJie HUANG ZaiYin | 2011 | Chinese Science Bulletin2011，56，3： | 3 |
| 6 | Homomorphic MAC-Based Scheme against Pollution Attacks in Network Coding显示文摘Network coding can improve network throughput in large, but it is vulnerable to the data pollution attacks. In this paper, we propose an efficient homomorphic message authentication code (MAC) scheme with discrete logarithm to detect and locate the malicious nodes. We also prove the security property of the scheme theoretically. Its effectiveness is demonstrated, and overhead is analyzed through extensive experiments. | ZHANG Yujie CAI Ying LI Zhuo FAN Yanfang | 2013 | Wuhan University Journal of Natural Sciences2013，18，5： | 3 |
| 7 | Reducing energy loss via tuning energy levels of polymer acceptors for efficient all-polymer solar cells显示文摘The open-circuit voltage(Voc) of all-polymer solar cells(all-PSCs) is typically lower than 0.9 V even for the most efficient ones.Large energy loss is the main reason for limiting Voc and efficiency of all-PSCs. Herein, through materials design using electron deficient building blocks based on bithiophene imides, the lowest unoccupied molecular orbital(LUMO) energy levels of polymer acceptors can be effectively tuned, which resulted in a reduced energy loss induced by charge generation and recombination loss due to the suppressed charge-transfer(CT) state absorption. Despite a negligible driving force, all-PSC based on the polymer donor and acceptor combination with well-aligned energy levels exhibited efficient charge transfer and achieved an external quantum efficiency over 70% while maintaining a large Voc of 1.02 V, leading to a 9.21% efficiency. Through various spectroscopy approaches, this work sheds light on the mechanism of energy loss in all-PSCs, which paves an avenue to achieving efficient all-PSCs with large Voc and drives the further development of all-PSCs. | Huiliang Sun Bin Liu Jianwei Yu Xianshao Zou Guangye Zhang Yujie Zhang Wei Zhang Mengyao Su Qunping Fan Kun Yang Jianhua Chen He Yan Feng Gao Xugang Guo | 2020 | Science China Chemistry2020，63，12： | 2 |
| 8 | YTHDF2 is essential for spermatogenesis and fertility by mediating a wave of transcriptional transition in spermatogenic cells显示文摘The dynamic and reversible regulation roles of m^(6)A modification and the characterization of m^(6)A readers have provided new insights into spermatogenesis at the post-transcriptional level.YTHDF2,as an m^(6)A reader,has been reported to mediate the m^(6)A-containing transcript decay during the mouse oocyte maturation,embryonic stem cell differentiation,neural development,and zebrafish maternal-to-zygotic transition.However,the roles of YTHDF2 in mammalian spermatogenesis are uncertain.Here,we generated germ cell-specific Ythdf2 mutants(Ythdf2-vKO)at a C57BL/6J background and demonstrated that YTHDF2 is essential for mouse spermatogenesis and fertility.Ythdf2-vKO provides oligoasthenoteratozoospermia phenotype with increased apoptosis in germ cells.High-throughput RNA-seq analysis showed that a group of mRNAs is upregulated in Ythdf2-vKO mouse testis;further analysis and MeRIP-qPCR data showed that most of the upregulated genes in Ythdf2-vKO mouse testis are modified with m^(6)A and are YTHDF2 candidate binding genes.Interestingly,RNA-seq analysis combined with our previous single-cell transcriptomics data of mouse spermatogenesis pointed out the failure of a wave of transcript transition during the spermatogenesis of Ythdf2-vKO mice,which was confirmed by gene expression analysis using qPCR of diplotene spermatocytes and round spermatids obtained through fluorescence-activated cell sorting.Our study demonstrates the fundamental role of YTHDF2 during mouse spermatogenesis and provides a potential candidate for the diagnosis of male infertility with the oligoasthenoteratozoospermia syndrome. | Xinxi Zhao Zhen Lin Yong Fan Wenzhi Li Yujie Zhang Fei Li Tong Hong Hua Feng Minghan Tong Ningling Wang Yanping Kuang Qifeng Lyu | 2021 | Acta Biochimica et Biophysica Sinica2021，53，12： | 2 |
| 9 | Synthesis of selective PAK4 inhibitors for lung metastasis of lung cancer and melanoma cells显示文摘The p21 activated kinase 4(PAK4) is serine/threonine protein kinase that is critical for cancer progression.Guided by X-ray crystallography and structure-based optimization,we report a novel subseries of C-3-substituted 6-ethynyl-1 H-indole derivatives that display high potential and specificity towards group Ⅱ PAKs.Among these inhibitors,compound 55 exhibited excellent inhibitory activity and kinase selectivity,displayed superior anti-migratory and anti-invasive properties against the lung cancer cell line A549 and the melanoma cell line B16.Compound 55 exhibited potent in vivo antitumor metastatic efficacy,with over 80% and 90% inhibition of lung metastasis in A549 or B16-BL6 lung metastasis models,respectively.Further mechanistic studies demonstrated that compound 55 mitigated TGF-β1-induced epithelial-mesenchymal transition(EMT). | Peilu Song Fan Zhao Dahong Li Jiqiang Qu Miao Yao Yuan Su Hanxun Wang Miaomiao Zhou Yujie Wang Yinli Gao Feng Li Dongmei Zhao Fengjiao Zhang Yu Rao Mingyu Xia Haitao Li Jian Wang Maosheng Cheng | 2022 | Acta Pharmaceutica Sinica B2022，12，6： | 2 |
| 10 | A large-scale outdoor atmospheric simulation smog chamber for studying atmospheric photochemical processes:Characterization and preliminary application显示文摘Understanding the formation mechanisms of secondary air pollution is very important for the formulation of air pollution control countermeasures in China.Thus,a large-scale outdoor atmospheric simulation smog chamber was constructed at Chinese Research Academy of Environmental Sciences(the CRAES Chamber),which was designed for simulating the atmospheric photochemical processes under the conditions close to the real atmospheric environment.The chamber consisted of a 56-m^(3) fluorinated ethylene propylene(FEP) Teflon film reactor,an electrically-driven stainless steel alloy shield,an auxiliary system,and multiple detection instrumentations.By performing a series of characterization experiments,we obtained basic parameters of the CRAES chamber,such as the mixing ability,the background reactivity,and the wall loss rates of gaseous compounds(propene,NO,NO_(2),ozone) and aerosols(ammonium sulfate).Oxidation experiments were also performed to study the formation of ozone and secondary organic aerosol(SOA),including α-pinene ozonolysis,propene and 1,3,5-trimethylbenzene photooxidation.Temperature and seed effects on the vapor wall loss and SOA yields were obtained in this work:higher temperature and the presence of seed could reduce the vapor wall loss;SOA yield was found to depend inversely on temperature,and the presence of seed could increase SOA yield.The seed was suggested to be used in the chamber to reduce the interaction between the gas phase and chamber walls.The results above showed that the CRAES chamber was reliable and could meet the demands for investigating tropospheric chemistry. | Junling Li Hong Li Xuezhong Wang Weigang Wang Maofa Ge Hao Zhang Xin Zhang Kun Li Yan Chen Zhenhai Wu Fahe Chai Fan Meng Yujing Mu Abdelwahid Mellouki Fang Bi Yujie Zhang Lingyan Wu Yongchun Liu | 2021 | Journal of Environmental Sciences2021，33，4： | 2 |
| 11 | Methods and timing of biliary drainage for acute cholangitis: Tokyo Guidelines显示文摘 | Masato Nagino Tadahiro Takada Yoshifumi Kawarada Yuji Nimura Yuichi Yamashita Toshio Tsuyuguchi Keita Wada Toshihiko Mayumi Masahiro Yoshida Fumihiko Miura Steven M. Strasberg Henry A. Pitt Jacques Belghiti Sheung-Tat Fan Kui-Hin Liau Giulio Belli Xiao-Pi | 2007 | Journal of Hepato-Biliary-Pancreatic Surgery2007，，1： | 2 |
| 12 | A potential tunable plasma photonic crystal: applications of atmospheric patterned gas discharge显示文摘 | FAN Weili DONG Lifang YANG Yujie | 2009 | IEEE Transations on Plasma Science2009，37，6： | 1 |
| 13 | Antimicrobial therapy for acute cholecystitis: Tokyo Guidelines显示文摘 | Masahiro Yoshida Tadahiro Takada Yoshifumi Kawarada Atsushi Tanaka Yuji Nimura Harumi Gomi Masahiko Hirota Fumihiko Miura Keita Wada Toshihiko Mayumi Joseph S. Solomkin Steven Strasberg Henry A. Pitt Jacques Belghiti Eduardo de Santibanes Sheung-Tat Fan M | 2007 | Journal of Hepato-Biliary-Pancreatic Surgery2007，，1： | 1 |
| 14 | Mixed secondary chromatin structure revealed by modeling radiation-induced DNA fragment length distribution显示文摘Spatial chromatin structure plays fundamental roles in many vital biological processes including DNA replication, transcription,damage and repair. However, the current understanding of the secondary structure of chromatin formed by local nucleosomenucleosome interactions remains controversial, especially for the existence and conformation of 30 nm structure. Since chromatin structure influences the fragment length distribution(FLD) of ionizing radiation-induced DNA strand breaks, a 3D chromatin model fitting FLD patterns can help to distinguish different models of chromatin structure. Here, we developed a novel '30-C' model combining 30 nm chromatin structure models with Hi-C data, which measured the spatial contact frequency between different loci in the genome. We first reconstructed the 3D coordinates of the 25 kb bins from Hi-C heatmaps. Within the25 kb bins, lower level chromatin structures supported by recent studies were filled. Simulated FLD patterns based on the 30-C model were compared to published FLD patterns induced by heavy ion radiation to validate the models. Importantly, the 30-C model predicted that the most probable chromatin fiber structure for human interphase fibroblasts in vivo was 45% zig-zag 30 nm fibers and 55% 10 nm fibers. | Wenzong Ma Chenyang Gu Lin Ma Caoqi Fan Chao Zhang Yujie Sun Cheng Li Gen Yang | 2020 | Science China(Life Sciences)2020，63，6： | 1 |
| 15 | Research on the Recognition Method of Electric Energy Meter Lead Title based on the Fuzzy Image Processing显示文摘 | WeiCun FAN Yujie LI | 2015 | International Journal of Technology Management2015，，6： | 1 |
| 16 | Methods and timing of biliary drainage for acute cholangitis: Tokyo Guidelines显示文摘 | Masato Nagino Tadahiro Takada Yoshifumi Kawarada Yuji Nimura Yuichi Yamashita Toshio Tsuyuguchi Keita Wada Toshihiko Mayumi Masahiro Yoshida Fumihiko Miura Steven M. Strasberg Henry A. Pitt Jacques Belghiti Sheung-Tat Fan Kui-Hin Liau Giulio Belli Xiao-Pi | 2007 | Journal of Hepato - Biliary - Pancreatic Surgery2007，，1： | 1 |
| 17 | Fractionation and characterization of ?-poly-l-lysine from Streptomyces albulus CGMCC 1986显示文摘 | JIA Shiru FAN Baoqing DAI Yujie | 2010 | Food Science and Biotechnology2010，19，2： | 1 |
| 18 | Surgical treatment of patients with acute cholecystitis: Tokyo Guidelines显示文摘 | Yuichi Yamashita Tadahiro Takada Yoshifumi Kawarada Yuji Nimura Masahiko Hirota Fumihiko Miura Toshihiko Mayumi Masahiro Yoshida Steven Strasberg Henry A. Pitt Eduardo de Santibanes Jacques Belghiti Markus W. Büchler Dirk J. Gouma Sheung-Tat Fan Serafin C | 2007 | Journal of Hepato - Biliary - Pancreatic Surgery2007，，1： | 1 |
| 19 | Surgical treatment of patients with acute cholecystitis: Tokyo Guidelines显示文摘 | Yuichi Yamashita Tadahiro Takada Yoshifumi Kawarada Yuji Nimura Masahiko Hirota Fumihiko Miura Toshihiko Mayumi Masahiro Yoshida Steven Strasberg Henry A. Pitt Eduardo de Santibanes Jacques Belghiti Markus W. Büchler Dirk J. Gouma Sheung-Tat Fan Serafin C | 2007 | Journal of Hepato - Biliary - Pancreatic Surgery2007，，1： | 1 |
| 20 | Surgical treatment of patients with acute cholecystitis: Tokyo Guidelines显示文摘 | Yuichi Yamashita Tadahiro Takada Yoshifumi Kawarada Yuji Nimura Masahiko Hirota Fumihiko Miura Toshihiko Mayumi Masahiro Yoshida Steven Strasberg Henry A. Pitt Eduardo de Santibanes Jacques Belghiti Markus W. Büchler Dirk J. Gouma Sheung-Tat Fan Serafin C | 2007 | Journal of Hepato - Biliary - Pancreatic Surgery2007，，1： | 1 |
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