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| 1 | Intravenous vs intraperitoneal mesenchymal stem cells administration:What is the best route for treating experimental colitis?显示文摘AIM:To investigate the therapeutic effects of mesenchymal stem cells(MSCs)transplanted intraperitoneally andintravenously in a murine model of colitis.METHODS:MSCs were isolated from C57BL/6 mouse adipose tissue.MSC cultures were analyzed according to morphology,cellular differentiation potential,and surface molecular markers.Experimental acute colitis was induced in C57BL/6 mice by oral administration of2%dextran sulfate sodium(DSS)in drinking water ad libitum from days 0 to 7.Colitis mice were treated with1×106 MSCs via intraperitoneal or intravenous injection on days 2 and 5.The disease activity index was determined daily based on the following parameters:weight loss,stool consistency and presence of blood in the feces and anus.To compare morphological and functional differences in tissue regeneration between different MSC injection modalities,mice were euthanized on day 8,and their colons were examined for length,weight,and histopathological changes.Inflammatory responses were determined by measuring the levels of different serum cytokines using a CBA Th1/Th2/Th17 kit.Apoptotic rates were evaluated by terminal deoxynucleotidyl transferase-mediated d UDPbiotin nick end labeling assay.RESULTS:Intravenous infusion of MSCs was more effective than intraperitoneal treatment(P<0.001)in reducing the clinical and histopathologic severity of colitis,which includes weight loss,diarrhea and inflammation.An histological evaluation demonstrated decreased colonic inflammation based on reduced crypt loss and reduced infiltration of inflammatory cells.This therapeutic effect was most likely mediated by the down-regulation of pro-inflammatory cytokines[interleukin(IL)-6 and tumor necrosis factor(TNF)];and by the up-regulation of anti-inflammatory cytokines(IL-10 and IL-4).Intravenous transplantation alsoinduced high levels of IFN that lead to activation of the immunosuppressive activity of the MSCs,which did not occur with intraperitoneal transplantation(P=0.006).An increase in apoptotic T cells was observed after intravenous,but not intraperitoneal,MSC infusion,suggesting that MSCs can induce apoptosis in resistant T cells in colonic inflammation(P=0.027).CONCLUSION:Our results demonstrate that intravenous treatment is a superior method for reducing colon inflammation compared with intraperitoneal therapy. | Fabiany da Costa Goncalves Natália Schneider Fernanda Otesbelgue Pinto Fabíola Schons Meyer Fernanda Visioli Bianca Pfaffenseller Patrícia Luciana da Costa Lopez Eduardo Pandolfi Passos Elizabeth Obino Cirne-Lima Luíse Meurer Ana Helena Paz | 2014 | World Journal of Gastroenterology2014，20，48： | 4 |
| 2 | Murine model to study brain,behavior and immunity during hepatic encephalopathy显示文摘AIM:To propose an alternative model of hepatic encephalopathy(HE) in mice,resembling the human features of the disease.METHODS:Mice received two consecutive intraperitoneal injections of thioacetamide(TAA) at low dosage(300 mg/kg).Liver injury was assessed by serum transaminase levels(ALT) and liver histology(hematoxylin and eosin).Neutrophil infiltration was estimated by confocal liver intravital microscopy.Coagulopathy was evaluated using prolonged prothrombin and partial thromboplastin time.Hemodynamic parameters were measured through tail cuff.Ammonia levels were quantified in serum and brain samples.Electroencephalography(EEG) and psychomotor activity score were performed to show brain function.Brain edema was evaluated using magnetic resonance imaging.RESULTS:Mice submitted to the TAA regime developed massive liver injury,as shown by elevation of serum ALT levels and a high degree of liver necrosis.An intense hepatic neutrophil accumulation occurred in response to TAA-induced liver injury.This led to mice mortality and weight loss,which was associated with severe coagulopathy.Furthermore,TAA-treated mice presented with increased serum and cerebral levels of ammonia,in parallel with alterations in EEG spectrum and discrete brain edema,as shown by magnetic resonance imaging.In agreement with this,neuropsychomotor abnormalities ensued 36 h after TAA,fulfilling several HE features observed in humans.In this context of liver injury and neurological dysfunction,we observed lung inflammation and alterations in blood pressure and heart rate that were indicative of multiple organ dysfunction syndrome.CONCLUSION:In summary,we describe a new murine model of hepatic encephalopathy comprising multiple features of the disease in humans,which may provide new insights for treatment. | Lindisley Ferreira Gomides Pedro Elias Marques Bruno Engler Faleiros Rafaela Vaz Pereira Sylvia Stella Amaral Thais Reis Lage Gustavo Henrique Souza Resende Patricia Alves Maia Guidine Giselle Foureaux Fabíola Mara Ribeiro Fabiana Paiva Martins Marco Antonio Peliky Fontes Anderson José Ferreira Remo Castro Russo Mauro Martins Teixeira Márcio Flávio Moraes Antonio Lúcio Teixeira Gustavo Batista Menezes | 2014 | World Journal of Hepatology2014，6，4： | 2 |
| 3 | Prenatal diagnosis of left ventricular aneurysm and diverticulum 显示文摘 | Marcia FAB Silvio HB Cristiane NB | 2009 | Arq Bras Cardiol2009，93，2： | 1 |
| 4 | Ceramic Technology of the Asurini do Xingu, Brazil : An Ethnoarchaeological Study of Artifact Variability显示文摘 | Fabíola Andréa Silva | 2008 | Journal of Archaeological Method and Theory2008，，3： | 1 |
| 5 | Experimental in vivo and in vitro models of multiple sclerosis: EAE and beyond显示文摘 | Markus Kipp Baukje van der Star Daphne Y.S. Vogel Fabìola Puentes Paul van der Valk David Baker Sandra Amor | 2012 | Multiple Sclerosis and Related Disorders2012，，1： | 1 |
| 6 | Anaphylactic reaction to lychee fruit:evidence for sensitization to profilin显示文摘 | Fab J Wuthrich B Vitehs S | 1995 | Clin Exp Allergy1995，25，10： | 1 |
| 7 | Content-based, Collaborative Recommen- dation显示文摘 | Fab S Y | 2000 | Communication of the ACM2000，40，3： | 1 |
| 8 | Solid pseudopapillary tumorsof the pancreas:diagnosis and curative treatment 显示文摘 | Frago R Fab regat J Jorha R | 2006 | Rev EspEnferm Dig2006，98，11： | 1 |
| 9 | Solomon L:A comparison of the prevalence of hallux valgus in three South African population显示文摘 | Gottschalk FAB Sallis JG Beighton PH | | 0，，： | 1 |
| 10 | Solomon L:The prewlence of hallux valgus in three South African population显示文摘 | Gottschalk FAB Beighton PH | | 0，，： | 1 |
| 11 | Beighton PH:The prevalence of hallux valgus in South African males显示文摘 | Gottschalk FAB Solomon L | | 0，，： | 1 |
| 12 | Tourist experience and wetland parks: a case of zhejiang, China显示文摘 | Wang Wanfei Chen Joseph S’ Fab Lingling Lu Jiaying | 2012 | Annals of Tourism Research2012，39，4： | 1 |
| 13 | The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla显示文摘 | Jaillon O Aury J M Noel B PolicritiA Clepet C Casagrande A Choisne N Aubourg S Vitulo N Jubin C Vezzi A Legeai F Hugueney P DasilvaC Horner D Mica E JublotD PoulainJ BruyereC BillaultA Segurens B GouyvenouxM Ugarte E Cattonaro F Anthouard V Vico V Del Fab | | 0，，： | 1 |
| 14 | Use of piezocone test to predict the axial capacity of driven and jacked pile in clays显示文摘 | Almeida MSS Danziger FAB Lunne T | 1996 | Canadian Geotechnical Journal1996，42，： | 1 |
| 15 | Validation of the Century model to estimate the impact of agriculture on soil organic carbon in Southern Brazil显示文摘 | Elisandra Solange Oliveira Bortolon Jo?o Mielniczuk Carlos Gustavo Tornquist Fabíola Lopes Homero Bergamaschi | 2011 | Geoderma2011，，： | 1 |
| 16 | Synergistic antibiotic activity of volatile compounds from the essential oil of Lippia sidoides and thymol显示文摘 | Helenicy N.H. Veras Fabíola F.G. Rodrigues Aracélio V. Colares Irwin R.A. Menezes Henrique D.M. Coutinho Marco A. Botelho José G.M. Costa | 2011 | Fitoterapia2011，，3： | 1 |
| 17 | Oxidation of Roading Asphalts显示文摘 | Henington P R At rick J E Geonge FAB | 1994 | Ind Eng Chem Res1994，33，11： | 1 |
| 18 | Oxidation of roading asphalts显示文摘 | Henington PR At rick JE Geonge FAB | 1994 | Ind Eng Chem Res1994，33，11： | 1 |
| 19 | The design of noncircular gear of constant pressure angle and the realization of its wire-electrode cutting 显示文摘 | ZUO D W FAB L CHEN R F ET AL | 2006 | Key Engineering Materials2006，，： | 1 |
| 20 | Beneficial Effects Of Lactobacillus Plantarum On Glycemia And Homocysteine Levels In Postmenopausal Women With Metabolic Syndrome: A Pilot Study显示文摘 | Fabíola Málaga Barreto Andréa Name Colado Sim?o Helena Kaminami Morimoto Marcell Alysson Batisti Lozovoy Isaias Dichi Lúcia Helena da Silva Miglioranza | 2013 | Nutrition2013，，： | 1 |
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