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| 1 | Differentiation of Mesenchymal Stem Cells Into Dopaminergic Neuron-like Cells in vitro显示文摘To explore the way to induce mesenchymal stem cells (MSCs) to differentiate into dopaminergic neurons in vitro. Methods MSCs were obtained from rat bone marrow, cultured and passaged. MSCs used in this experiment had multipotency, which was indirectly proved by being induced to differentiate into chondrocytes and adipocytes. MSCs were cultured in medium containing 0.5 mmol/L IBMX for 2 days. Then the medium was replaced with induction medium, which contained GDNF, IL-1β, mesencephalic glial-cell-conditioned medium and flash-frozen mesencephalic membrane fragments. The surface markers of the differentiated neurons, such as NSE, nestin, MAP-2a, b and TH were detected by immunocytochemistry and Western blot after MSCs were cultured in induction medium for 7 days and 15 days. Results MSCs differentiated into neural progenitors and expressed nestin after MSCs were incubated with medium containing IBMX for 2 d. After the medium was replaced with induction medium containing many inducing agents, MSCs differentiated into neuron-like cells and dopaminergic neuron-like cells and expressed NSE, MAP-2a, b and TH. The percentage of NSE-positive cells, MAP-2a, b-positive cells and TH-positive cells was 30.032±2.489%, 41.580±5.101% and 34.958±5.534%, respectively after MSCs were induced in medium containing GDNF, IL-1β, mesencephalic glial-cell-conditioned medium and flash-frozen mesencephalic membrane fragments for 15 days. Conclusion MSCs can differentiate into dopaminergic neuron-like cells and are a new cell source for the treatment of neurodegeneration diseases and have a great potential for wide | L1GUO FEIYIN HONG-QIMENG LINGLING TA-NAHU-HE PENGLI CHUN-XIAZHANG SHUNYU DE-SHENGDUAN HONG-XUEFAN | 2005 | Biomedical and Environmental Sciences2005，18，1： | 31 |
| 2 | Submarine Landslide Identified in DLW3102 Core of the Northern Continental Slope, South China Sea显示文摘In this paper, we take DLW3101 core obtained at the top of the canyon(no landslide area) and DLW3102 core obtained at the bottom of the canyon(landslide area) on the northern continental slope of the South China Sea as research objects. The chronostratigraphic framework of the DLW3101 core and elemental strata of the DLW3101 core and the DLW3102 core since MIS5 are established by analyzing oxygen isotope, calcium carbonate content, and X-Ray Fluorescence(XRF) scanning elements. On the basis of the information obtained by analyzing the sedimentary structure and chemical elements in the landslide deposition, we found that the DLW3102 core shows four layers of submarine landslides, and each landslide layer is characterized by high Si, K, Ti, and Fe contents, thereby indicating terrigenous clastic sources. L1(2.15–2.44 m) occurred in MIS2, which is a slump sedimentary layer with a small sliding distance and scale. L2(15.48–16.00 m) occurred in MIS5 and is a debris flow-deposited layer with a scale and sliding distance that are greater than those of L1. L3(19.00–20.90 m) occurred in MIS5; its upper part(19.00–20.00 m) is a debris flow-deposited layer, and its lower part(20.00–20.90 m) is a sliding deposition layer. The landslide scale of L3 is large. L4(22.93–24.27 m) occurred in MIS5; its upper part(22.93–23.50 m) is a turbid sedimentary layer, and its lower part(23.50–24.27m) is a slump sedimentary layer. The landslide scale of L4 is large. | XU Yuanqin LIU Lejun ZHOU Hang HUANG Baoqi LI Ping MA Xiudong DONG Feiyin | 2018 | Journal of Ocean University of China2018，17，1： | 2 |
| 3 | Synthesis and Spectral Properties of Novel Water-soluble Near-infrared Fluorescent Indocyanines显示文摘Two fluorescent pentamethine and a squarylium indocyanines containing at least one p-carboxybenzyl group on N atoms in the heterocyclic rings were synthesized. They had good water solubility and photostability. Their maximum absorption and maximum emission were 600-700 nm in water. When it was anchored onto nanostructured TiO2 electrode, compared with in water, the squaraine showed double absorption peaks (one blue shifted and another red shifted) and absorption intensity of the red shift peak increased with the increase of the time of irradiation. The intensity of the blue one decreased simultaneously. We proposed that the presence of two electronic charge forms of squaraine anchored on the TiO2 film might be the reason. | LiQiuWANG XiaoJunPENG WeiBingZHANG FeiYIN JingNanCUI XinQinGAO | 2005 | Chinese Chemical Letters2005，16，3： | 1 |
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