| 7 | Human Wharton’s jelly mesenchymal stem cells inhibit cytokine storm in acute respiratory distress syndrome in a rat model显示文摘Objective:To evaluate the potential effect of human Wharton’s jelly mesenchymal stem cells(hWJMSCs)on acute respiratory distress syndrome in lipopolysaccharide(LPS)-induced rats.Methods:The hWJMSCs(5×10^(4)/mL,5×10^(5)/mL,5×10^(6)/mL)were administered to rats on day 1 and day 8 after being induced by LPS(5 mg/kg body weight).TNF-αlevels in the lung and IL-18 and IL-1βlevels in the serum were measured using ELISA.In addition,caspase-1 expression in lung tissues was quantified using qRT-PCR,and NF-κB and IL-6 expressions were assessed using immunohistochemistry.Results:The hWJMSCs decreased TNF-αlevels in the lung and plasma IL-18 and IL-1βlevels.Moreover,the hWJMSCs downregulated the expressions of caspase-1,IL-6,and NF-κB in lung tissues.Conclusions:The hWJMSCs can decrease inflammatory markers of acute respiratory distress syndrome in a rat model and may be further investigated for the treatment of acute respiratory distress syndrome. | Wahyu Widowati Teresa Liliana Wargasetia Fanny Rahardja Rimonta F Gunanegara Didik Priyandoko Marisca Evalina Gondokesumo Ervi Afifah Cahyaning Riski Wijayanti Rizal Rizal | 2022 | Asian Pacific Journal of Tropical Biomedicine2022，12，8： | 0 |
| 8 | Anti-inflammatory properties of oolong tea(Camellia sinensis)ethanol extract and epigallocatechin gallate in LPS-induced RAW 264.7 cells显示文摘Objective: To evaluate the anti-inflammatory activity of oolong tea ethanol extract(OTEE) and epigallocatechin gallate(EGCG) on lipopolysaccharide-induced murine macrophage cell line(RAW 264.7).Methods: A cytotoxic assay using MTS tetrazolium was conducted to find a nontoxic level of OTEE and EGCG toward RAW 264.7 cells. Interleukins(IL-6, IL-1 b), tumor necrosis factor-a(TNF-a), and cyclooxigenase-2(COX-2) levels were measured by ELISA, and nitric oxide(NO) levels measured by a nitrate/nitrite colorimetric assay to determine the inhibition activity of OTEE and EGCG.Results: Lipopolysaccharide induction increases NO, COX-2, IL-6, IL-1 b, and TNF-a levels compared with the untreated cell(negative control). The positive control,lipopolysaccharide-induced RAW 264.7 without treatments showed the highest level of all pro-inflammatory cytokines and modulators tested in this study. The positive control was used as standard to obtain OTEE and EGCG inhibition activity toward NO, COX-2,IL-6, IL-1 b, and TNF-a. OTEE had a higher inhibition activity toward NO, COX-2, IL-6,and IL-1 b than EGCG; the reverse was seen for TNF-a. However, both OTEE and EGCG suppressed production of NO, COX-2, IL-6, IL-1 b, and TNF-a.Conclusions: OTEE and EGCG have the potential for use as anti-inflammatory drugs,which is shown by their ability to reduce the production of NO, COX-2, IL-6, IL-1 b, and TNF-a in active macrophages. | Arina Novilla Dedi Somantri Djamhuri Betty Nurhayati Dwi Davidson Rihibiha Ervi Afifah Wahyu Widowati | 2017 | Asian Pacific Journal of Tropical Biomedicine2017，7，11： | 0 |
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