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| 1 | Bone marrow transdifferentiation in brain after transplantation: a retrospective study显示文摘 | Christopher R Cogle Anthony T Yachnis Eric D Laywell Dani S Zander John R Wingard Dennis A Steindler Edward W Scott | 2004 | The Lancet . 2004 (9419)2004，，9419： | 1 |
| 2 | A Phase Ⅱ Randomized Clinical Trial of Intravitreal Bevacizumab for Diabetic Macular Edema显示文摘 | Scott I U Edwards A R Beck R W | 2007 | Ophthalmology2007，114，10： | 1 |
| 3 | Brief Psychotherapies for Depression:Current Status显示文摘 | Jan Scott Edward W | 2004 | Curr Opin Psychiatry2004，17，1： | 1 |
| 4 | Rare long-term survivors of pancreatic adenocarcinoma without curative resection显示文摘Long-term outcome data in pancreatic adenocarcinoma are predominantly based on surgical series, as resection is currently considered essential for longterm survival. In contrast, five-year survival in nonresected patients has rarely been reported. In this report, we examined the incidence and natural history of ≥ 5-year survivors with non-resected pancreatic adenocarcinoma. All patients with pancreatic adenocarcinoma who received oncologic therapy alone without surgery at our institution between 1995 and 2009 were identified. Non-resected ≥ 5-year survivors represented 2%(11/544) of all non-resected patients undergoing treatment for pancreatic adenocarcinoma, and 11%(11/98) of ≥ 5-year survivors. Nine patients had localized tumor and 2 metastatic disease at initial diagnosis. Disease progression occurred in 6 patients, and the local tumor bed was the most common site of progression. Six patients suffered from significant morbidities including recurrent cholangitis, second malignancy, malnutrition and bowel perforation. A rare subset of patients with pancreatic cancer achieve longterm survival without resection. Despite prolonged survival, morbidities unrelated to the primary cancer were frequently encountered and a close follow-up is warranted in these patients. Factors such as tumor biology and host immunity may play a key role in disease progression and survival. | Stephen Y Oh Alicia Edwards Margaret T Mandelson Bruce Lin Russell Dorer W Scott Helton Richard A Kozarek Vincent J Picozzi | 2015 | World Journal of Gastroenterology2015，21，48： | 1 |
| 5 | Greenhouse gas,animal performance,and bacterial population structure responses to dietary monensin fed to dairy cows显示文摘 | Scott W H Edward J D | | 0，，： | 1 |
| 6 | Intcrannual and seasonal variation in fluxes of water and carbon dioxide from a riparian woodland ecosystem 显示文摘 | Scott R L Edwards E A Shuttleworth W J | 2004 | Agricultural and Forest Meteorology2004，122，12： | 1 |
| 7 | Medical Preparedness for a Terrorist Incident Involving Chemical and Biological Agents During the 1996 Atlanta Olympic Games显示文摘 | Trueman W Sharp Richard J Brennan Mark Keim R.Joel Williams Edward Eitzen Scott Lillibridge | 1998 | Annals of Emergency Medicine1998，，2： | 1 |
| 8 | Connective tissue growth factor differentially binds to members of the cystine knot superfamily and potentiates platelet-derived growth factor-B signaling in rabbit corneal fibroblast cells显示文摘AIM:To study the binding of connective tissue growth factor(CTGF) to cystine knot-containing ligands and how this impacts platelet-derived growth factor(PDGF)-B signaling. METHODS:The binding strengths of CTGF to cystine knot-containing growth factors including vascular en-dothelial growth factor(VEGF)-A,PDGF-B,bone morphogenetic protein(BMP)-4,and transforming growth factor(TGF)-β1 were compared using the LexA-based yeast two-hybrid system. EYG48 reporter strain that carried a wild-type LEU2 gene under the control of Lex A operators and a lac Z reporter plasmid(p80plac Z) containing eight high affinity Lex A binding sites were used in the yeast two-hybrid analysis. Interactions between CTGF and the tested growth factors were evaluated based on growth of transformed yeast cells on selective media and colorimetric detection in a liquid β-galactosidase activity assay. Dissociation constants of CTGF to VEGF-A isoform 165 or PDGF-BB homo-dimer were measured in surface plasma resonance(SPR) analysis. CTGF regulation in PDGF-B presentation to the PDGF receptor β(PDGFRβ) was also quantitatively assessed by the SPR analysis. Combinational effects of CTGF protein and PDGF-BB on activation of PDGFRβ and downstream signaling molecules ERK1/2 and AKT were assessed in rabbit corneal fibroblast cells by Western analysis. RESULTS:In the LexA-based yeast two-hybrid system,cystine knot motifs of tested growth factors were fused to the activation domain of the transcriptional factor GAL4 while CTGF was fused to the DNA binding domain of the bacterial repressor protein Lex A. Yeast cotransformants containing corresponding fusion proteins for CTGF and all four tested cystine knot motifs survived on selective medium containing galactose and raffinose but lacking histidine,tryptophan,and uracil. In liquid β-galactosidase assays,CTGF expressing cells that were co-transformed with the cystine knot of VEGF-A had the highest activity,at 29.88 ± 0.91 fold above controls(P < 0.01). Cells containing the cystine knot of BMP-4 expressed the second most activity,with a 24.77 ± 0.47 fold increase(P < 0.01). Cells that contained the cystine knot of TGF-β1 had a 3.80 ± 0.66 fold increase(P < 0.05) and the ones with the cystine knot of PDGF-B had a 2.64 ± 0.33 fold increase of β-galactosidase activity(P < 0.01). Further SPR analysis showed that the association rate between VEGF-A 165 and CTGF was faster than PDGF-BB and CTGF. The calculated dissociation constant(KD) of CTGF to VEGF165 and PDGF-BB was 1.8 and 43 nmol/L respectively. PDGFBB ligand and PDGFRβ receptor formed a stable complex with a low dissociation constant 1.4 nmol/L. Increasing the concentration of CTGF up to 263.2 nmol/L significantly the ligand/receptor binding. In addition,CTGF potentiated phosphorylation of PDGFRβ and AKT in rabbit corneal fibroblast cells stimulated by PDGFBB in tissue culture condition. In contrast,CTGF did not affect PDGF-B induced phosphorylation of ERK1/2.CONCLUSION:CTGF has a differential binding affinity to VEGF-A,PDGF-B,BMP-4,and TGF-β. Its weak association with PDGF-B may represent a novel mechanism to enhance PDGF-B signaling. | Liya Pi Pei-Yu Chung Sriniwas Sriram Masmudur M Rahman Wen-Yuan Song Edward W Scott Bryon E Petersen Gregory S Schultz | 2015 | World Journal of Biological Chemistry2015，6，4： | 0 |
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