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| 1 | 三氯生对离体鹅尾臀腺和人乳腺癌细胞脂肪酸合成酶的抑制作用显示文摘背景与目的:研究表明,人乳腺癌早期和病程中癌细胞脂肪酸合成酶(fattyacidsynthase,FAS)呈高效表达。抑制FAS可引起癌细胞凋亡。本研究采用酶促反应动力学实验观察三氯生对离体鹅尾臀腺FAS的影响,建立实验方法,研究三氯生对人乳腺癌SKBr3细胞FAS的抑制作用。方法:运用超速离心和SuperdexPG200层析技术分离纯化鹅尾臀腺FAS,用超速离心技术部分纯化人乳腺癌SKBr3细胞FAS。不同浓度三氯生与FAS相互作用不同时间后,加入底物,用分光光度法观察三氯生对FAS的抑制作用。结果:在鹅尾臀腺组,FAS被纯化为SDS-PAGE电泳单条区带(分子量250kDa),三种浓度三氯生(12.5、25.0、100.0μmol/L)与FAS在催化作用前相互作用0、5和10min,对FAS的抑制率分别为26.40%、28.30%和43.93%(12.5μmol/L);46.22%、50.28%和97.05%(25μmol/L);98.11%、97.75%和97.37%(100μmol/L)。在人乳腺癌SKBr3细胞组FAS被部分纯化,25、50、100和200μmol/L三氯生分别与FAS在催化前相互作用5min,对FAS活性的抑制率分别为20.00%、26.67%、60.00%和100.00%。结论:三氯生对离体鹅尾臀腺FAS和人乳腺癌SKBr3细胞FAS均具有抑制作用;作用强度既依赖于抑制剂浓度,又依赖于抑制剂与酶相互作用时间。 | 王映强 赖炳森 Vernon E.Anderson | 2003 | 癌症2003，22，3： | 5 |
| 2 | 腰靠支撑对驾驶人腰部负载影响的研究显示文摘为提高驾驶人的乘坐舒适性,本文中对汽车座椅腰靠进行研究。基于驾驶人肌肉骨骼生物力学模型,对坐姿驾驶人在第三腰椎部位处3种不同凸出量(0,2和4cm)的腰靠支撑条件下,仿真求解了腰部椎间关节力和肌肉负载。结果表明,4cm凸出量的腰靠支撑下,驾驶人腰部的椎间关节力平均下降8%、肌肉负载平均下降15%,而2cm凸出量的腰靠支撑却使腰部椎间关节力和肌肉负载均略有升高。本文从腰部载荷的角度解释了腰靠凸出量对改善乘坐舒适性影响的内在机理,所提出的仿真方法可实现不同腰靠支撑下腰部载荷的定量评估,为座椅腰靠的舒适性设计提供指导。 | 孟祥杰 王文军 Dennis E.Anderson Alexander G.Bruno 成波 | 2016 | 汽车工程2016，38，2： | 3 |
| 3 | The Mercantilist Index of Trade Policy*显示文摘 | James E.Anderson J. PeterNeary | 2003 | International Economic Review2003，，2： | 2 |
| 4 | E‐satisfaction and e‐loyalty: A contingency framework显示文摘 | Rolph E.Anderson Srini S.Srinivasan | 2003 | Mark2003，，2： | 2 |
| 5 | Genetic Evidence of Middle East Respiratory Syndrome Coronavirus(MERS-Cov) and Widespread Seroprevalence among Camels in Kenya显示文摘We describe the first genome isolation of Middle East respiratory syndrome coronavirus(MERS-CoV) in Kenya. This fatal zoonotic pathogen was first described in the Kingdom of Saudi Arabia in 2012. Epidemiological and molecular evidence revealed zoonotic transmission from camels to humans and between humans. Currently, MERS-CoV is classified by the WHO as having high pandemic potential requiring greater surveillance. Previous studies of MERS-CoV in Kenya mainly focused on site-specific and archived camel and human serum samples for antibodies. We conducted active nationwide cross-sectional surveillance of camels and humans in Kenya, targeting both nasal swabs and plasma samples from 1,163 camels and 486 humans collected from January 2016 to June 2018. A total of 792 camel plasma samples were positive by ELISA. Seroprevalence increased with age, and the highest prevalence was observed in adult camels(82.37%, 95%confidence interval(CI) 79.50–84.91). More female camels were significantly seropositive(74.28%, 95% CI 71.14–77.19)than male camels(P \ 0.001)(53.74%, 95% CI 48.48–58.90). Only 11 camel nasal swabs were positive for MERS-CoV by reverse transcription-quantitative PCR. Phylogenetic analysis of whole genome sequences showed that Kenyan MERSCoV clustered within sub-clade C2, which is associated with the African clade, but did not contain signature deletions of orf4 b in African viruses. None of the human plasma screened contained neutralizing antibodies against MERS-CoV. This study confirms the geographically widespread occurrence of MERS-CoV in Kenyan camels. Further one-health surveillance approaches in camels, wildlife, and human populations are needed. | Sheila Ommeh Wei Zhang Ali Zohaib Jing Chen Huajun Zhang Ben Hu Xing-Yi Ge Xing-Lou Yang Moses Masika Vincent Obanda Yun Luo Shan Li Cecilia Waruhiu Bei Li Yan Zhu Desterio Ouma Vincent Odendo Lin-Fa Wang Danielle E.Anderson Jacqueline Lichoti Erick Mungube Francis Gakuya Peng Zhou Kisa-Juma Ngeiywa Bing Yan Bernard Agwanda Zheng-Li Shi | 2018 | Virologica Sinica2018，33，6： | 1 |
| 6 | Gravity with Gravitas:A Solution to the Border Puzzle显示文摘 | James E.Anderson Van Wincoop Eric | | 0，，01： | 1 |
| 7 | A Theoretical Foundation for the Gravity Equation显示文摘 | James E.Anderson | | 0，，01： | 1 |
| 8 | Calcitonin gene‐related peptide regulates gene transcription in primary afferent neurons显示文摘 | L. E.Anderson V. S.Seybold | 2004 | Journal of Neurochemistry2004，，6： | 1 |
| 9 | 关于引力模型的拓展:对边境之谜的一个解释显示文摘引力方程是经济学在实证研究上所取得的最伟大的成功之一。它将双边贸易流量与GDP、距离和其他影响贸易壁垒的因素联系起来。该方程波广泛运用于推断如关税同盟、汇率机制、民族纽带、语言文化等体系的贸易流动效应。与常理相悖,该实证性的引力方程并不具备理论基础。由Ander Son[1979]首次提出的理论告诉我们:在控制了经济规模之后, | James E.Anderson Eric van Wincoop 王思婷 胡朝霞 | 2006 | 经济资料译丛2006，，4： | 1 |
| 10 | The Multinational Corporation's Degree of Control over Foreign Subsidiaries:An Empirical Test of a Transaction Cost Explanation显示文摘 | H.Gatignon E.Anderson | | 0，，02： | 1 |
| 11 | On the development of optical peripheral nerve interfaces显示文摘Limb loss and spinal cord injury are two debilitating conditions that continue to grow in prevalence. Prosthetic limbs and limb reanimation present two ways of providing affected individuals with means to interact in the world. These techniques are both dependent on a robust interface with the peripheral nerve. Current methods for interfacing with the peripheral nerve tend to suffer from low specificity, high latency and insufficient robustness for a chronic implant. An optical peripheral nerve interface may solve some of these problems by decreasing invasiveness and providing single axon specificity. In order to implement such an interface three elements are required:(1) a transducer capable of translating light into a neural stimulus or translating neural activity into changes in fluorescence,(2) a means for delivering said transducer and(3) a microscope for providing the stimulus light and detecting the fluorescence change. There are continued improvements in both genetically encoded calcium and voltage indicators as well as new optogenetic actuators for stimulation. Similarly, improvements in specificity of viral vectors continue to improve expression in the axons of the peripheral nerve. Our work has recently shown that it is possible to virally transduce axons of the peripheral nerve for recording from small fibers. The improvements of these components make an optical peripheral nerve interface a rapidly approaching alternative to current methods. | Hans E.Anderson Richard F.ff.Weir | 2019 | Neural Regeneration Research2019，14，3： | 1 |
| 12 | What is the Point of Equality显示文摘 | E.Anderson | | 0，，： | 1 |
| 13 | Risk factors for urinary bladder carcinoma in postmenopausal women显示文摘 | ApekshaTripathi Aaron R.Folsom Kristin E.Anderson | 2002 | Cancer2002，，： | 1 |
| 14 | Effect of cardiopulmonary bypass on glucose homeostasis after coronary artery bypass surgery显示文摘 | Russell E.Anderson Kerstin Brismar Gunilla Barr | | 0，，： | 1 |
| 15 | E‐satisfaction and e‐loyalty: A contingency framework显示文摘 | Rolph E.Anderson Srini S.Srinivasan | 2003 | Psychol Mark2003，，2： | 1 |
| 16 | Inhibition of LFA‐1/ICAM‐1 and VLA‐4/VCAM‐1 as a therapeutic approach to inflammation and autoimmune diseases显示文摘 | HelenaYusuf‐Makagiansar Meagan E.Anderson Tatyana V.Yakovleva Joseph S.Murray Teruna J.Siahaan | 2002 | Med Res Rev2002，，2： | 1 |
| 17 | Signaling satellite‐cell activation in skeletal muscle: Markers, models, stretch, and potential alternate pathways显示文摘 | Ashley C.Wozniak JimingKong ErikaBock OrestPilipowicz Judy E.Anderson | 2005 | Muscle Nerve2005，，3： | 1 |
| 18 | E‐satisfaction and e‐loyalty: A contingency framework显示文摘 | Rolph E.Anderson Srini S.Srinivasan | 2003 | Psychol Mark2003，，2： | 1 |
| 19 | 刈割频率和烧荒对天然牧草长势的影响显示文摘刈割频率和烧荒可以影响柳枝稷(Panicum virgatum L.)、大须芒草(Andropogon gerardiiVitman)和黄假高粱(Sorghastrum nutans L.Nash)的长势。1986年进行了一项田间研究,从1988~1991年的3,4和5月进行烧荒处理,同时进行3次刈割处理。每年在相同的样区进行上述处理。处理为再裂区排列,完全随机区组设计。大须芒草春季的黄化生物量分别比柳枝稷和黄假高粱高147%和122%。刈割管理对牧草长势的影响在一个生长季节之后发生,但在后续的研究中发现这种影响随之变小。黄化生物量在刈割2次到3次之间降幅较大,而在刈割1次到2次之间则较小。大须芒草产生的分蘖枝分别比柳枝稷和黄假高粱多95%和33%。对所有牧草和刈割频率而言,烧荒刺激产生的分蘖平均为32%。刈割频率增加了分蘖密度,但是,随着刈割频率的增加,通过黄化生长测定的牧草长势却降低了。这表明,随着频繁的采摘,这些牧草的分蘖是以消耗贮备能量为代价的。旺盛的春季黄化生长和较高的分蘖潜力是大须芒草在普列里草原占优势的原因之一。 | Greg J.Cuomo Bruce E.Anderson Linda J.Young 刘学录 | 1998 | 国外畜牧学(草原与牧草)1998，，4： | 1 |
| 20 | E‐satisfaction and e‐loyalty: A contingency framework显示文摘 | Rolph E.Anderson Srini S.Srinivasan | 2003 | Psychol Mark2003，，2： | 1 |
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