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| 1 | Sinomenine ester derivative inhibits glioblastoma by inducing mitochondria-dependent apoptosis and autophagy by PI3K/AKT/mTOR and AMPK/mTOR pathway显示文摘Glioblastoma multiforme(GBM)in the central nervous system is the most lethal advanced glioma and currently there is no effective treatment for it.Studies of sinomenine,an alkaloid from the Chinese medicinal plant,Sinomenium acutum,showed that it had inhibitory effects on several kinds of cancer.Here,we synthesized a sinomenine derivative,sino-wcj-33(SW33),tested it for antitumor activity on GBM and explored the underlying mechanism.SW33 significantly inhibited proliferation and colony formation of GBM and reduced migration and invasion of U87 and U251 cells.It also arrested the cell cycle at G2/M phase and induced mitochondria-dependent apoptosis.Differential gene enrichment analysis and pathway validation showed that SW33 exerted anti-GBM effects by regulating PI3 K/AKT and AMPK signaling pathways and significantly suppressed tumorigenicity with no obvious adverse effects on the body.SW33 also induced autophagy through the PI3 K/AKT/mTOR and AMPK/mTOR pathways.Thus,SW33 appears to be a promising drug for treating GBM effectively and safely. | Xiangjin Zheng Wan Li Huanli Xu Jinyi Liu Liwen Ren Yihui Yang Sha Li Jinhua Wang Tengfei Ji Guanhua Du | 2021 | Acta Pharmaceutica Sinica B2021，11，11： | 15 |
| 2 | Genistein suppresses the mitochondrial apoptotic pathway in hippocampal neurons in rats with Alzheimer's disease显示文摘Genistein is effective against amyloid-β toxicity,but the underlying mechanisms are unclear.We hypothesized that genistein may protect neurons by inhibiting the mitochondrial apoptotic pathway,and thereby play a role in the prevention of Alzheimer's disease.A rat model of Alzheimer's disease was established by intraperitoneal injection of D-galactose and intracerebral injection of amyloid-β peptide(25–35).In the genistein treatment groups,a 7-day pretreatment with genistein(10,30,90 mg/kg) was given prior to establishing Alzheimer's disease model,for 49 consecutive days.Terminal deoxyribonucleotidyl transferase-mediated d UTP nick end labeling assay demonstrated a reduction in apoptosis in the hippocampus of rats treated with genistein.Western blot analysis showed that expression levels of capase-3,Bax and cytochrome c were decreased compared with the model group.Furthermore,immunohistochemical staining revealed reductions in cytochrome c and Bax immunoreactivity in these rats.Morris water maze revealed a substantial shortening of escape latency by genistein in Alzheimer's disease rats.These findings suggest that genistein decreases neuronal loss in the hippocampus,and improves learning and memory ability.The neuroprotective effects of genistein are associated with the inhibition of the mitochondrial apoptotic pathway,as shown by its ability to reduce levels of caspase-3,Bax and cytochrome c. | Yan Wang Biao Cai Jing Sha Ting-ting Wang Run-ze Cai Chang-ju Ma Tao Han Jun Du | 2016 | Neural Regeneration Research2016，11，7： | 12 |
| 3 | Evapotranspiration and Its Energy Exchange in Alpine Meadow Ecosystem on the Qinghai-Tibetan Plateau显示文摘To understand the water and energy exchange on the Qinghai-Tibetan Plateau, we explored the characteristics of evapotranspiration (ET) and energy fluxes from 2002 to 2005 over a Kobresia meadow ecosystem using the eddy covariance method. The ratio of annual ET to precipitation (P) of meadow ecosystem was about 60%, but varied greatly with the change of season from summer to winter. The annual ET/P in meadow was lower than that in shrub, steppe and wetland ecosystems of this plateau. The incident solar radiation (R s ) received by the meadow was obviously higher than that of lowland in the same latitude; however the ratio of net radiation (R n ) to R s with average annual value of 0.44 was significantly lower than that in the same latitude. The average annual ET was about 390 mm for 2002-2005, of which more than 80% occurred in growing season from May to September. The energy consumed on the ET was about 44% of net radiation in growing season, which was lower than that of shrub, steppe and wetland on this plateau. This study demonstrates that the Kobresia meadow may prevent the excessive water loss through evapotranspiration from the ecosystem into the atmosphere in comparison to the shrub, steppe and wetland ecosystems of the Qinghai-Tibetan Plateau. | LI Jie JIANG Sha WANG Bin JIANG Wei-wei TANG Yan-hong DU Ming-yuan GU Song | 2013 | Journal of Integrative Agriculture2013，12，8： | 10 |
| 4 | Single-cell RNA sequencing reveals atlas of dairy goat testis cells显示文摘Single-cell RNA sequencing(scRNA-seq)is useful for exploring cell heterogeneity.For large animals,however,little is known regarding spermatogonial stem cell(SSC)selfrenewal regulation,especially in dairy goats.In this study,we described a high-resolution scRNA-seq atlas derived from a dairy goat.We identified six somatic cell and five spermatogenic cell subtypes.During spermatogenesis,genes with significantly changed expression were mainly enriched in the Notch,TGF-β,and Hippo signaling pathways as well as the signaling pathway involved in the regulation of stem cell pluripotency.We detected and screened specific candidate marker genes(TKTL1 and AES)for spermatogonia.Our study provides new insights into goat spermatogenesis and the development of testicular somatic cells. | Xiu-Wei Yu Tong-Tong Li Xiao-Min Du Qiao-Yan Shen Meng-Fei Zhang Yu-Dong Wei Dong-Hui Yang Wen-Jing Xu Wen-Bo Chen Chun-Ling Bai Xue-Ling Li Guang-Peng Li Na Li Sha Peng Ming-Zhi Liao Jin-Lian Hua | 2021 | Zoological Research2021，42，4： | 9 |
| 5 | A Rapid Analysis Tool for Aerodynamics/Aerothermodynamics of Hypersonic Vehicles显示文摘A rapid engineering surface panel method to analyze aerodynamics and aerothermodynamics of hypersonic vehicles is developed.To obtain the surface pressure distribution of a hypersonic vehicle,the local surface inclination method is applied to calculate the pressure coefficient for each surface panel element,of which the normal vector is corrected first by using an efficient data structure and Rey-casting algorithm,local Reynolds numbers are calculated according to the geometric streamline method,then the aerodynamic heating flux is computed by both reference enthalpy relations and Reynolds analogy method.Several typical test cases are performed and the results indicate that,the developed tool is effective in predicting the aerodynamics/aerothermodynamics for complex geometry of hypersonic vehicle in a wide range of Mach numbers with a sufficient accuracy. | Li Zhengzhou Xiao Tianhang Lü Fanxi Yu Xiongqing Zhang Zhenming Du Sha | 2017 | Transactions of Nanjing University of Aeronautics and Astronautics2017，34，4： | 7 |
| 6 | Circulating MicroRNAs as Novel Diagnostic Biomarkers for Very Early-onset(≤40 years) Coronary Artery Disease显示文摘Objective Very early-onset coronary artery disease(CAD) is a great challenge in cardiovascular medicine throughout the world,especially regarding its early diagnosis.This study explored whether circulating micro RNAs(mi RNAs) could be used as potential biomarkers for patients with very early-onset CAD.Methods We performed an initial screening of mi RNA expression using RNA isolated from 20 patients with angiographically documented very early-onset CAD and 20 age-and sex-matched normal controls.For further confirmation,we prospectively examined the mi RNAs selected from 40 patients with very early-onset CAD and 40 angiography-normal controls.Results A total of 22 overexpressed mi RNAs and 22 underexpressed mi RNAs were detected in the initial screening.RT-q PCR analysis of the mi RNAs obtained from the initial screening revealed that four mi RNAs including mi R-196-5p,mi R-3163-3p,mi R-145-3p,and mi R-190a-5p exhibited significantly decreased expression in patients compared with that in controls(P<0.05).The areas under the receiver operating characteristic curve for these mi RNAs were 0.824(95% CI,0.731-0.917;P<0.001),0.758(95% CI,0.651-0.864;P<0.001),0.753(95% CI,0.643-0.863;P<0.001),and 0.782(95% CI,0.680-0.884;P<0.001),respectively,in the validation set.Conclusion To our knowledge,this is an advanced study to report about four serum mi RNAs(mi R-196-5p,mi R-3163-3p,mi R-145-3p,and mi R-190a-5p) that could be used as novel biomarkers for the diagnosis of very early-onset CAD. | DU Ying YANG Sheng Hua LI Sha CUI Chuan Jue ZHANG Yan ZHU Cheng Gang GUO Yuan Lin WU Na Qiong GAO Ying SUN Jing DONG Qian LIU Geng LI Jian Jun | 2016 | Biomedical and Environmental Sciences2016，29，8： | 5 |
| 7 | A sperm GPI-anchored protein elicits sperm-cumulus cross-talk leading to the acrosome reaction显示文摘 | Yin, L. Chung, C. M. Huo, R. Liu, H. Zhou, C. Xu, W. Zhu, H. Zhang, J. Shi, Q. Wong, H. Y.C. Chen, J. Lu, Y. Bi, Y. Zhao, C. Du, Y. Ma, M. Cai, Y. Chen, W. Y. Fok, K. L. Tsang, L. L. Li, K. Ni, Y. Chung, Y. W. Zhou, Z. Sha, J. Chan, H. C. | 2009 | 南京医科大学学报(自然科学版)2009，29，4： | 5 |
| 8 | Systematic analyses of glutamine and glutamate metabolisms across different cancer types显示文摘Background: Glutamine and glutamate are known to play important roles in cancer biology. However, no detailed information is available in terms of their levels of involvement in various biological processes across different cancer types, whereas such knowledge could be critical for understanding the distinct characteristics of different cancer types. Our computational study aimed to examine the functional roles of glutamine and glutamate across different cancer types.Methods: We conducted a comparative analysis of gene expression data of cancer tissues versus normal control tissues of 11 cancer types to understand glutamine and glutamate metabolisms in cancer. Specifically, we developed a linear regression model to assess differential contributions by glutamine and/or glutamate to each of seven biological processes in cancer versus control tissues.Results: While our computational predictions were consistent with some of the previous observations, multiple novel predictions were made:(1) glutamine is generally not involved in purine synthesis in cancer except for breast cancer, and is similarly not involved in pyridine synthesis except for kidney cancer;(2) glutamine is generally not involved in ATP production in cancer;(3) glutamine's contribution to nucleotide synthesis is minimal if any in cancer;(4) glutamine is not involved in asparagine synthesis in cancer except for bladder and lung cancers; and(5) glutamate does not contribute to serine synthesis except for bladder cancer.Conclusions: We comprehensively predicted the roles of glutamine and glutamate metabolisms in selected metabolic pathways in cancer tissues versus control tissues, which may lead to novel approaches to therapeutic development targeted at glutamine and/or glutamate metabolism. However, our predictions need further functional validation. | Yuan Tian Wei Du Sha Cao Yue Wu Ning Dong Yan Wang Ying Xu | 2017 | Chinese Journal of Cancer2017，36，12： | 5 |
| 9 | Dmrt1 regulates the immune response by repressing the TLR4 signaling pathway in goat male germline stem cells显示文摘Double sex and mab-3-related transcription factor 1(Dmrt1),which is expressed in goat male germline stem cells(mGSCs)and Sertoli cells,is one of the most conserved transcription factors involved in sex determination.In this study,we highlighted the role of Dmrt1 in balancing the innate immune response in goat mGSCs.Dmrt1 recruited promyelocytic leukemia zinc finger(Plzf),also known as zinc finger and BTB domain-containing protein 16(Zbtb16),to repress the Toll-like receptor 4(TLR4)-dependent inflammatory signaling pathway and nuclear factor(NF)-κB.Knockdown of Dmrt1 in seminiferous tubules resulted in widespread degeneration of germ and somatic cells,while the expression of proinflammatory factors were significantly enhanced.We also demonstrated that Dmrt1 stimulated proliferation of mGSCs,but repressed apoptosis caused by the immune response.Thus,Dmrt1 is sufficient to reduce inflammation in the testes,thereby establishing the stability of spermatogenesis and the testicular microenvironment. | Yu-Dong Wei Xiao-Min Du Dong-Hui Yang Fang-Lin Ma Xiu-Wei Yu Meng-Fei Zhang Na Li Sha Peng Ming-Zhi Liao Guang-Peng Li Chun-Ling Bai Wei-Shuai Liu Jin-Lian Hua | 2021 | Zoological Research2021，42，1： | 5 |
| 10 | Efficacy and economic analysis of Ex- PRESS implantation versus trabeculectomy in uncontrolled glaucoma: a systematic review and Meta-analysis显示文摘AIM:To systematically review the current evidence based on the efficacy and cost of Ex-PRESS implantation and trabeculectomy(Trab)for uncontrolled glaucoma.·METHODS:Clinical trials were identified by electronic databases(Pub Med,EMBASE,ISI Web of science and Cochrane library),and data,such as intraocular pressure(IOP),the complete and qualified success rate,the postoperative complications and the cost,were exacted from these relevant studies.Weighted mean difference(WMD),odds ratio(OR)and 95%confidence intervals(CIs)were calculated and were pooled using a randomeffects model.·RESULTS:Eleven relevant publications and two abstracts met the inclusion criteria.The efficacy of ExPRESS was similar to that of Trab in the percentage of IOP reduction(IOPR%)at 1,2y(WMD:-2.01;95%CI:-7.92-3.90;P=0.50 and WMD:2.89;95%CI:-8.05-13.83;P=0.60,respectively).Ex-PRESS possessed a significant higher complete and qualified success rate(OR:1.59;95%CI:1.07-2.35;P=0.02 and OR:1.74;95%CI:1.06-2.86;P=0.03,respectively).Moreover,Ex-PRESS exerted a significantly lower frequency of hypotony and hyphema than Trab(OR:0.39;95%CI:0.21-0.72;P=0.003 and OR:0.27;95%CI:0.10-0.69;P=0.003,respectively).However,there was no consistent result on the cost between the two groups according to the previous three studies.·CONCLUSION:Both Trab and Ex-PRESS have equivalent efficacy in lowering IOP,yet Ex-PRESS had a lower risk of hypotony and hyphema than Trab.Nevertheless,whether the cost of Ex-PRESS was less than that of Trab should be further investigated to ensure evidence-based conclusion in the long run. | Ling Wang Fang Sha Da-Dong Guo Hong-Sheng Bi Jun-Kang Si Yu-Xiang Du Kai Tang | 2016 | International Journal of Ophthalmology(English edition)2016，9，1： | 5 |
| 11 | A User Requirement Oriented Web Service Discovery Approach Based on Logic and Threshold Petri Net显示文摘In recent years, the number of Web services has increased significantly. Web service discovery has drawn much attention with the development of Web service applications and big data analysis. Under this circumstance, traditional Web service discovery strategies cannot adequately meet high user requirements due to the efficiency and precision of service discovery is low. In order to improve the accuracy and efficiency of service discovery, a user requirement oriented Web service discovery approach based on Petri nets is proposed in this study. A data preprocessing strategy of Web service is first designed. Then, a service clustering method is proposed based on Petri nets, which can conduct service cluster head generation, service cluster composition, and service discovery. The proposed method utilizes a superior data preprocessing method. Using simulation experiments,the efficiency and precision of Web service discovery are illustrated. Finally, the application value of the approach on real Web service is discussed. | Jing Sha Yuyue Du Liang Qi | 2019 | IEEE/CAA Journal of Automatica Sinica2019，6，6： | 5 |
| 12 | Effectiveness and safety of Ginkgo biloba extract(GBE50)in the treatment of dizziness caused by cerebral arteriosclerosis:a multi-center,double-blind,randomized controlled trial显示文摘OBJECTIVE:To evaluate the effectiveness and safety of Ginkgo biloba extract(GBE50)in the treatment of dizziness caused by cerebral arteriosclerosis.METHODS:This was a multi-center,double-blind,double-dummy,positive-controlled,parallel randomized controlled clinical trial with 1∶1 allocation.We recruited 404 patients with dizziness caused by cerebral arteriosclerosis(blood stasis symptom pattern)in 10 hospitals in China.GBE50 group received GBE50 and Naoxinqing tablet(NXQ)of mimetic agent,control group received NXQ and GBE50 of mimetic agent.The main outcome was Traditional Chinese Medicine(TCM)symptom pattern score of blood stasis after 6 weeks.The secondary outcomes were changes in the dizziness handicap inventory(DHI)score,vertigo visual analogue scale(VAS)score,the university of California vertigo questionnaire(UCLA-DQ)score and single-item symptom score of TCM from baseline to 2,4 and 6 weeks.Safety indicators included the incidence of adverse events,severe adverse events and laboratory examination including blood routine,liver function,renal function,and so forth.RESULTS:The total effective rate of TCM symptom pattern score in the GBE50 group after 6 weeks of treatment was higher than that in the control group,the difference in rate was statistically significant(92.67%vs 83.07%,P=0.004).Compared with the control group,there was no difference in the incidence of adverse reactions(9.95%vs 14.85%,P=0.136).CONCLUSION:The treatment of dizziness caused by cerebral arteriosclerosis with GBE50 is effective,safe and reliable. | SHA Rina TANG Lu DU Yawei WU Shengxian SHI Huawei ZOU Hongxin ZHANG Xuran DONG Xinglu ZHOU Li | 2022 | Journal of Traditional Chinese Medicine2022，42，1： | 5 |
| 13 | Research Progress of the Antiviral Bioactivities of Natural Flavonoids显示文摘Flavonoids are now considered as an indispensable component in a variety of nutraceutical and pharmaceutical applications.Most recent researches have focused on the health aspects of flavonoids for humans.Especially,different flavonoids have been investigated for their potential antiviral activities,and several natural flavonoids exhibited significant antiviral properties both in vitro and in vivo.This review provides a survey of the literature regarding the evidence for antiviral bioactivities of natural flavonoids,highlights the cellular and molecular mechanisms of natural flavonoids on viruses,and presents the details of most reported flavonoids.Meanwhile,future perspectives on therapeutic applications of flavonoids against viral infections were discussed. | Lin Wang Junke Song Ailin Liu Bin Xiao Sha Li Zhang Wen Yang Lu Guanhua Du | 2020 | Natural Products and Bioprospecting2020，10，5： | 4 |
| 14 | Relationship of lipid and lipoprotein ratios with coronary severity in patients with new on-set coronary artery disease complicated with type 2 diabetics显示文摘BackgroundDiabetes mellitus (DM ) 与冠的动脉疾病(CAD ) 被联系前进。尽管以前的研究与 CAD 表明了类脂化合物和脂蛋白比率的协会,没有数据有关在类脂化合物和脂蛋白比率和在糖尿病患者的新发作 CAD 的严厉之间的关系当前是可得到的。因此,现在的学习的目的有新发作 CAD 的 380 个连续 T2DM 病人是在与类型 2 DM (T2DM ).MethodsA 在病人预言 CAD 的严厉调查类脂化合物和脂蛋白比率的实用性全部的在现在的学习被注册。然后,他们根据 Gensini 被分类进三个组分数(GS ) tertiles。在类脂化合物和脂蛋白比率之间的关系当前使用了, GS 与到 apoA 的 apolipoprotein B 是自然转变木头的 GS ( lnGS )的 investigated.ResultsPositive 关联--我比率( apoB/apoA-I ), non-high-density 脂蛋白胆固醇到 apoA --我比率( non-HDL-C/apoA-I ),并且低密度的脂蛋白胆固醇到 apoA --我比率( LDL-C/apoA-I )被发现( r = 0.18 , 0.13 , 0.12 ,分别地所有 P < ;0.05 ) 。Multivariate 逻辑分析为高 GS 作为最强壮的预言者显示了 apoB/apoA-I (或 = 5.67, 95% CI:1.45-23.92, P = 0.003 ) 。在操作 apoB/apoA-I 的典型曲线的接收装置下面的区域是 0.63 (95% CI:0.60-0.66, P = 0.001 ) 为预言高 GS。apoB/apoA-I 的最佳的截止值预言高 GS 与 61.2% 的敏感和 62.1%.ConclusionsLipid 和脂蛋白比率的特性是 0.72 可能为在 T2DM 病人预言新发作 CAD 的严厉是有用的,并且 apoB/apoA-I 在这张人口作为最重要的预言者出现。 | Ying DU Juan CHEN Man-Hua CHEN Sheng-Hua YANG Sha LI Yuan-Lin GUO Cheng-Gang ZHU Rui-Xia XU Qian DONG Jian-Jun LI | 2016 | Journal of Geriatric Cardiology2016，13，8： | 4 |
| 15 | Tumorigenic bacteria in colorectal cancer:mechanisms and treatments显示文摘Colorectal cancer(CRC)is the third most common and the second most fatal cancer.In recent years,more attention has been directed toward the role of gut microbiota in the initiation and development of CRC.Some bacterial species,such as Fusobacterium nucleatum,Escherichia coli,Bacteroides fragilis,Enterococcus faecalis,and Salmonella sp.have been associated with CRC,based upon sequencing studies in CRC patients and functional studies in cell culture and animal models.These bacteria can cause host DNA damage by genotoxic substances,including colibactin secreted by pks+Escherichia coli,B.fragilis toxin(BFT)produced by Bacteroides fragilis,and typhoid toxin(TT)from Salmonella.These bacteria can also indirectly promote CRC by influencing host-signaling pathways,such as E-cadherin/β-catenin,TLR4/MYD88/NF-κB,and SMO/RAS/p38 MAPK.Moreover,some of these bacteria can contribute to CRC progression by helping tumor cells to evade the immune response by suppressing immune cell function,creating a proinflammatory environment,or influencing the autophagy process.Treatments with the classical antibacterial drugs,metronidazole or erythromycin,the antibacterial active ingredients,M13@Ag(electrostatically assembled from inorganic silver nanoparticles and the protein capsid of bacteriophage M13),berberine,and zerumbone,were found to inhibit tumorigenic bacteria to different degrees.In this review,we described progress in elucidating the tumorigenic mechanisms of several CRC-associated bacteria,as well as progress in developing effective antibacterial therapies.Specific bacteria have been shown to be active in the oncogenesis and progression of CRC,and some antibacterial compounds have shown therapeutic potential in bacteria-induced CRC.These bacteria may be useful as biomarkers or therapeutic targets for CRC. | Sha Li Jinyi Liu Xiangjin Zheng Liwen Ren Yihui Yang Wan Li Weiqi Fu Jinhua Wang Guanhua Du | 2022 | Cancer Biology & Medicine2022，19，2： | 3 |
| 16 | MELK is an oncogenic kinase essential for metastasis,mitotic progression,and programmed death in lung carcinoma显示文摘Lung cancer is the fastest growth rate of morbidity and mortality in nearly a decade,and remains difficult to treat.Furthermore,the molecular mechanisms underlying its development are still unclear.In this study,bioinformatics analysis showed that MELK was highly expressed in lung cancer and negatively correlated to the survival of lung adenocarcinoma(LUAD).Immunohistochemistry analysis of LUAD patient tissues revealed there were a high level of MELK expression in LUAD.Knockdown of MELK expression inhibits the migration and invasion of LUAD cells,which may be mediated by Twist1,Slug,MMP7,and N-catenin.Overexpression of MELK promoted the growth of LUAD cells in medium,3D Matrigel,and nude mice.Inhibition of MELK by OTSSP167 arrested cycle of LUAD cells at G2/M phase via PLK1-CDC25C-CDK1 pathway,and triggered apoptosis-mediated pyroptosis.Together,these data indicate that MELK is critical for metastasis,mitotic progression,and programmed death of LUAD and may be a promising therapeutic target for LUAD. | Qin Tang Wan Li Xiangjin Zheng Liwen Ren Jinyi Liu Sha Li Jinhua Wang Guanhua Du | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 3 |
| 17 | Benzimidazoles induce concurrent apoptosis and pyroptosis of human glioblastoma cells via arresting cell cycle显示文摘Glioblastoma multiforme(GBM)is the most malignant and lethal primary brain tumor in adults accounting for about 50%of all gliomas.The only treatment available for GBM is the drug temozolomide,which unfortunately has frequent drug resistance issue.By analyzing the hub genes of GBM via weighted gene co-expression network analysis(WGCNA)of the cancer genome atlas(TCGA)dataset,and using the connectivity map(CMAP)platform for drug repurposing,we found that multiple azole compounds had potential anti-GBM activity.When their anti-GBM activity was examined,however,only three benzimidazole compounds,i.e.flubendazole,mebendazole and fenbendazole,potently and dose-dependently inhibited proliferation of U87 and U251 cells with IC50 values below 0.26μM.Benzimidazoles(0.125−0.5μM)dose-dependently suppressed DNA synthesis,cell migration and invasion,and regulated the expression of key epithelial-mesenchymal transition(EMT)markers in U87 and U251 cells.Benzimidazoles treatment also dose-dependently induced the GBM cell cycle arrest at the G2/M phase via the P53/P21/cyclin B1 pathway.Furthermore,the drugs triggered pyroptosis of GBM cells through the NF-κB/NLRP3/GSDMD pathway,and might also concurrently induced mitochondria-dependent apoptosis.In a nude mouse U87 cell xenograft model,administration of flubendazole(12.5,25,and 50 mg·kg^(−1)·d^(−1),i.p,for 3 weeks)dose-dependently suppressed the tumor growth without obvious adverse effects.Taken together,our results demonstrated that benzimidazoles might be promising candidates for the treatment of GBM. | Li-wen Ren Wan Li Xiang-jin Zheng Jin-yi Liu Yi-hui Yang Sha Li Sen Zhang Wei-qi Fu Bin Xiao Jin-hua Wang Guan-hua Du | 2022 | Acta Pharmacologica Sinica2022，43，1： | 3 |
| 18 | Protection of 3'-methoxy-puerarin against focal brain ischemia and its association with c-fos expression显示文摘The present study established a rat model of focal brain ischemia by occlusion of the middle cerebral artery covered with FeCl3, and investigated the protective effect of 3'-methoxy-puerarin. Hippocampal and cortical c-fos gene expression was determined using in situ hybridization. Results showed that 3'-methoxy-puerarin reduced neurological deficit scores, cerebral infarcted zone and water content of brain tissues, dramatically increased the activity of catalase and glutathione peroxidase in the ischemia zone of the hippocampus, increased the activity of catalase in the cortex, decreased lipid peroxide and lactic acid contents in the hippocampus and cerebral cortex, and down-regulated c-fos gene expression in brain ischemic rats. Results demonstrated that 3'-methoxy-puerarin exhibited cerebroprotective effects against focal brain ischemia, which involved c-fos gene expression. | Sha Liu Yibing Zhang Guiyou Du Yong Zhao Haifeng Cui Chunyu Cao | 2011 | Neural Regeneration Research2011，6，27： | 2 |
| 19 | Association of assisted reproductive technology, germline de novo mutations and congenital heart defects in a prospective birth cohort study显示文摘Emerging evidence suggests that children conceived through assisted reproductive technology(ART)have a higher risk of congenital heart defects(CHDs)even when there is no family history.De novo mutation(DNM)is a well-known cause of sporadic congenital diseases;however,whether ART procedures increase the number of germline DNM(gDNM)has not yet been well studied.Here,we performed whole-genome sequencing of 1137 individuals from 160 families conceived through ART and 205 families conceived spontaneously.Children conceived via ART carried 4.59 more gDNMs than children conceived spontaneously,including 332 paternal and 1.26 maternal DNMs,after correcting for parental age at conception,cigarette smoking,alcohol drinking,and exercise behaviors.Paternal DNMs in offspring conceived via ART are characterized by C>T substitutions at CpG sites,which potentially affect protein-coding genes and are significantly associated with the increased risk of CHD.In addition,the accumulation of non-coding functional mutations was independently associated with CHD and 87.9% of the mutations were originated from the father.Among ART offspring,infertility of the father was associated with elevated paternal DNMs;usage of both recombinant and urinary follicle-stimulating hormone and high-dosage human chorionic gonadotropin trigger was associated with an increase of maternal DNMs.In sum,the increased gDNMs in offspring conceived by ART were primarily originated from fathers,indicating that ART itself may not be a major reason for the accumulation of gDNMs.Our findings emphasize the importance of evaluating the germline status of the fathers in families with the use of ART. | Cheng Wang Hong Lv Xiufeng Ling Hong Li Feiyang Diao Juncheng Dai Jiangbo Du Ting Chen Qi Xi Yang Zhao Kun Zhou Bo Xu Xiumei Han Xiaoyu Liu Meijuan Peng Congcong Chen Shiyao Tao Lei Huang Cong Liu Mingyang Wen Yangqian Jiang Tao Jiang Chuncheng Lu Wei Wu Di Wu Minjian Chen Yuan Lin Xuejiang Guo Ran Huo Jiayin Liu Hongxia Ma Guangfu Jin Yankai Xia Jiahao Sha Hongbing Shen Zhibin Hu | 2021 | Cell Research2021，31，8： | 2 |
| 20 | Taking the pulse of COVID-19:a spatiotemporal perspective显示文摘The sudden outbreak of the Coronavirus disease(COVID-19)swept across the world in early 2020,triggering the lockdowns of several billion people across many countries,including China,Spain,India,the U.K.,Italy,France,Germany,Brazil,Russia,and the U.S.The transmission of the virus accelerated rapidly with the most confirmed cases in the U.S.,India,Russia,and Brazil.In response to this national and global emergency,the NSF Spatiotemporal Innovation Center brought together a taskforce of international researchers and assembled implementation strategies to rapidly respond to this crisis,for supporting research,saving lives,and protecting the health of global citizens.This perspective paper presents our collective view on the global health emergency and our effort in collecting,analyzing,and sharing relevant data on global policy and government responses,human mobility,environmental impact,socioeconomical impact;in developing research capabilities and mitigation measures with global scientists,promoting collaborative research on outbreak dynamics,and reflecting on the dynamic responses from human societies. | Chaowei Yang Dexuan Sha Qian Liu Yun Li Hai Lan Weihe Wendy Guan Tao Hu Zhenlong Li Zhiran Zhang John Hoot Thompson Zifu Wang David Wong Shiyang Ruan Manzhu Yu Douglas Richardson Luyao Zhang Ruizhi Hou You Zhoua Cheng Zhong Yifei Tian Fayez Beaini Kyla Carte Colin Flynn Wei Liu Dieter Pfoser Shuming Bao Mei Li Haoyuan Zhang Chunbo Liu Jie Jiang Shihong Du Liang Zhao Mingyue Lu Lin Li Huan Zhou Andrew Ding | 2020 | International Journal of Digital Earth2020，13，10： | 2 |
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