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| 1 | CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes显示文摘染色体编辑工具例如定期聚类短 palindromic 重复(CRISPR ) 联系了的 interspaced 系统(Cas ) 广泛地被用来包括动物接合子和人的房间在模型系统修改基因,并且为基本研究和临床的应用保持巨大的诺言。迄今为止,严肃的知识差距在人的早胚胎,并且在效率和在人的培植前胚胎使用象 CRISPR/Cas9 那样的技术的潜在的离开目标效果留在我们 DNA 修理机制的理解。在这份报告,我们使用了推进的接合子调查 CRISPR/Cas9-mediated 基因在人的房间编辑的 tripronuclear (3PN ) 。我们发现 CRISPR/Cas9 能有效地劈开内长的 -globin 基因(HBB ) 。然而,相应再结合的效率指导了 HBB 的修理(HDR ) 是低的,编辑胚胎是马赛克。离开目标劈开在由 T7E1 试金并且 whole-exome 定序揭示了的这些 3PN 接合子也是明显的。而且,内长的 delta-globin 基因(HBD ) 对 HBB 相应,与外长的施主 oligos 竞争了充当修理模板,导致倔强的变化。我们的数据也显示在这些胚胎的 HBB 地点的修理通过非转线路 HDR 小径优先地发生了。一起拿,我们的工作加亮紧迫的需要进一步改进 CRISPR/Cas9 平台的忠实和特性,为编辑的 CRSIPR/Cas9-mediated 的任何临床的应用程序的一个前提。 | Puping Liang Yanwen Xu Xiya Zhang Chenhui Ding Rui Huang Zhen Zhang Jie Lv Xiaowei Xie Yuxi Chen Yujing Li Ying Sun Yaofu Bai Zhou Songyang Wenbin Ma Canquan Zhou Junjiu Huang | 2015 | Protein & Cell2015，6，5： | 149 |
| 2 | Towards 6G wireless communication networks:vision,enabling technologies,and new paradigm shifts显示文摘The fifth generation(5G)wireless communication networks are being deployed worldwide from 2020 and more capabilities are in the process of being standardized,such as mass connectivity,ultra-reliability,and guaranteed low latency.However,5G will not meet all requirements of the future in 2030 and beyond,and sixth generation(6G)wireless communication networks are expected to provide global coverage,enhanced spectral/energy/cost efficiency,better intelligence level and security,etc.To meet these requirements,6G networks will rely on new enabling technologies,i.e.,air interface and transmission technologies and novel network architecture,such as waveform design,multiple access,channel coding schemes,multi-antenna technologies,network slicing,cell-free architecture,and cloud/fog/edge computing.Our vision on 6G is that it will have four new paradigm shifts.First,to satisfy the requirement of global coverage,6G will not be limited to terrestrial communication networks,which will need to be complemented with non-terrestrial networks such as satellite and unmanned aerial vehicle(UAV)communication networks,thus achieving a space-airground-sea integrated communication network.Second,all spectra will be fully explored to further increase data rates and connection density,including the sub-6GHz,millimeter wave(mmWave),terahertz(THz),and optical frequency bands.Third,facing the big datasets generated by the use of extremely heterogeneous networks,diverse communication scenarios,large numbers of antennas,wide bandwidths,and new service requirements,6G networks will enable a new range of smart applications with the aid of artificial intelligence(AI)and big data technologies.Fourth,network security will have to be strengthened when developing 6G networks.This article provides a comprehensive survey of recent advances and future trends in these four aspects.Clearly,6G with additional technical requirements beyond those of 5G will enable faster and further communications to the extent that the boundary between physical and cyber worlds disappears. | Xiaohu YOU Cheng-Xiang WANG Jie HUANG Xiqi GAO Zaichen ZHANG Mao WANG Yongming HUANG Chuan ZHANG Yanxiang JIANG Jiaheng WANG Min ZHU Bin SHENG Dongming WANG Zhiwen PAN Pengcheng ZHU Yang YANG Zening LIU Ping ZHANG Xiaofeng TAO Shaoqian LI Zhi CHEN Xinying MA Chih-Lin I Shuangfeng HAN Ke LI Chengkang PAN Zhimin ZHENG Lajos HANZO Xuemin(Sherman)SHEN Yingjie Jay GUO Zhiguo DING Harald HAAS Wen TONG Peiying ZHU Ganghua YANG Jun WANG Erik GLARSSON Hien Quoc NGO Wei HONG Haiming WANG Debin HOU Jixin CHEN Zhe CHEN Zhangcheng HAO Geoffrey Ye LI Rahim TAFAZOLLI Yue GAO HVincent POOR Gerhard P.FETTWEIS Ying-Chang LIANG | 2021 | Science China(Information Sciences)2021，64，1： | 122 |
| 3 | The Genome of Dendrobium officinale Illuminates the Biology of the Important Traditional Chinese Orchid Herb显示文摘 | Liang Yan Xiao Wang Hui Liu Yang Tian Jinmin Lian Ruijuan Yang ShumeiHao Xuanjun Wang Shengchao Yang Qiye Li Shuai Qi Ling Kui Moses Okpekum Xiao Ma Jiajin Zhang Zhaoli Ding Guojie Zhang Wen Wang Yang Dong Jun Sheng | 2015 | Molecular Plant2015，8，6： | 64 |
| 4 | The human long non-coding RNA-RoR is a p53 repressor in response to DNA damage显示文摘在应力之上,肿瘤 suppressor p53 的水平显著地被提高,是众所周知的。然而尽管有广泛的研究,包含的内在的机制重要为导致压力的 p53 规定内部播放器仍然不充分被理解。我们人的 lincRNA-RoR (RoR ) 是 p53 的一个强壮的否定管理者的现在的证据。不同于通过 ubiquitin-proteasome 小径引起 p53 降级的 MDM2, RoR 通过和异构的原子 ribonucleoprotein 的直接相互作用压制 p53 翻译我(hnRNP 我) 。重要地,带 hnRNP 的一个 28 底 RoR 序列我有约束力的主题为 p53 压抑必要、足够。我们进一步证明 RoR 禁止调停 p53 的房间周期拘捕和 apoptosis。最后,我们表明 p53 transcriptionally 导致 RoR 表示的一个 RoR-p53 自动调整的反馈环。一起,这些结果建议 RoR-hnRNP I-p53 轴可以组成一个另外的监视网络让房间更好对各种各样的压力作出回应。 | Ali Zhang Nanjiang Zhou Jianguo Huang Qian Liu Koji Fukuda Ding Ma Zhaohui Lu Cunxue Bai Kounosuke Watabe Yin-Yuan Mo | 2013 | Cell Research2013，23，3： | 47 |
| 5 | Key techniques for 5G wireless communications:network architecture, physical layer,and MAC layer perspectives显示文摘The fourth generation(4G) mobile communication systems are offering service worldwide steadily.Although 4G systems could be loaded with much more services and data than previous systems, there is still a dramatic gap between the people's practical requirements and what can be offered by the 4G technologies.Consequently, the research and development for the fifth generation(5G) systems have already been started. This article presents an overview of potential network architecture and highlights several promising techniques which could be employed in the future 5G systems. These techniques include non-orthogonal multiple access(NOMA),massive multiple input and multiple output(MIMO), cooperative communications and network coding, full duplex(FD), device-to-device(D2D) communications, millimeter wave communications, automated network organization, cognitive radio(CR), and green communications. The state-of-art and implementation issue of these techniques are also addressed. | MA Zheng ZHANG ZhengQuan DING ZhiGuo FAN PingZhi LI HengChao | 2015 | Science China(Information Sciences)2015，58，4： | 49 |
| 6 | Molecular subtyping and genomic profiling expand precision medicine in refractory metastatic triple-negative breast cancer:the FUTURE trial显示文摘Triple-negative breast cancer(TNBC)is a highly heterogeneous disease,and molecular subtyping may result in improved diagnostic precision and targeted therapies.Our previous study classified TNBCs into four subtypes with putative therapeutic targets.Here,we conducted the FUTURE trial(ClinicalTrials.gov identifer:NCT03805399),a phaseⅠb/Ⅱsubtyping-based and genomic biomarker-guided umbrella trial,to evaluate the efficacy of these targets.Patients with refractory metastatic TNBC were enrolled and stratifed by TNBC subtypes and genomic biomarkers,and assigned to one of these seven arms:(A)pyrotinib with capecitabine,(B)androgen receptor inhibitor with CDK4/6 inhibitor,(C)anti PD-1 with nab-paclitaxel,(D)PARP inhibitor included,(E)and(F)anti-VEGFR included,or(G)mTOR inhibitor with nab paclitaxel.The primary end point was the objective response rate(ORR).We enrolled 69 refractory metastatic TNBC patients with a median of three previous lines of therapy(range,1-8).Objective response was achieved in 20(29.0%,95%confidence interval(CI):18.7%-41.2%)of the 69 intention-to-treat(ITT)patients.Our results showed that immunotherapy(arm C),in particular,achieved the highest ORR(52.6%,95%CI:28.9%-75.6%)in the ITT population.Arm E demonstrated favorable ORR(26.1%,95%C:10.2%-48.4%in the ITT population)but with more high grade(23)adverse events.Somatic mutations of TOP2A and CD8 immunohistochemical score may have the potential to predict immunotherapy response in the immunomodulatory subtype of TNBC.In conclusion,the phaseⅠb/ⅡFUTURE trial suggested a new concept for TNBC treatment,demonstrating the clinical beneft of subtyping-based targeted therapy for refractory metastatic TNBC. | Yi-Zhou Jiang Yin Liu Yi Xiao Xin Hu Lin Jiang Wen-ia Zuo Ding Ma Jiahan Ding Xiaoyu Zhu Jianjun Zou Claire Verschraegen Daniel G.Stover Virginia Kaklamani Zhong-Hua Wang Zhi-Ming Shao | 2021 | Cell Research2021，31，2： | 37 |
| 7 | IL-1beta sensitizes rat intervertebral disc cells to Fas ligand mediated apoptosis in vitro显示文摘瞄准:在老鼠上决定 recombinant 老鼠船边交货 Ligand 的 apoptotic 效果内部脊椎的磁盘房间在有教养的老鼠与船边交货的 IL-1beta 在试管内,和表达式预先对待内部脊椎的磁盘房间。方法:房间从 Sprague-Dawley 老鼠从腰部的磁盘的内部纤维环和移行区被孤立。房间在单层被种并且在 5 个处理组划分。IL-1beta (10 ng/mL ) , FasL (5, 20 ng/mL ) 与 / 没有 IL-1beta (10 ng/mL ) ,预告的处理分别地在 Dulbeccoos 被增加有 1% 胎儿的牛的浆液的修改 Eagleos 媒介和 Hamos 氟利昂十二媒介。在 32 h 以后,房间在 V-FITC 和 propidium 碘化物与附属建筑被染色用 RT-PCR 用流动血细胞计数并且到船边交货的分析抄写评估 apoptosis。结果:与控制组相比, FasL (20 ng/mL ) , IL-1beta (10 ng/mL )+FasL (5 ng/mL ) ,和 IL-1beta (10 ng/mL )+FasL (20 ng/mL ) 导致了圆盘房间(P<0.01 ) 的重要 apoptosis。Apoptosis 被 FasL 5 ng/mL (P<0.05 ) 也导致;而, apoptosis 没被 IL-1beta (10 ng/mL )(P>0.05 ) 导致。IL-1beta (10 ng/mL ) 在圆盘房间提高了 FasL (5 ng/mL ) 和 FasL (20 ng/mL ) 的导致 apoptosis 效果。在所有组的船边交货基因抄写和在 5 个处理组的船边交货表示是近似比控制组大的 1.2-2.1-fold (分别地, P<0.05 ) 。另外,在与 IL-1beta 预告的处理组一起的 FasL 的船边交货表示比在 FasL 组(P<0.01 ) 显著地起来调整。结论:这研究的结果证明圆盘房间与增加的 apoptotic 响应 FasL 在试管内评估的 IL-1beta 预先对待并且提供了卓见在将在退化圆盘由于发炎因素被提高的圆盘房间理解 Fas/FasL 调停系统的 apoptosis。 | Li-yang CUI Shang-li LIU Yue DING Dong-sheng HUANG Ruo-fan MA Wei-guo HUANG Bao-shan HU Qiu-hui PAN | 2007 | Acta Pharmacologica Sinica2007，28，10： | 32 |
| 8 | Contemporary crustal movement of continental China obtained by global positioning system(GPS) measurements显示文摘We obtain a unified horizontal velocity field of continental China and vicinity through measurements at 81 GPS stations provided by the National Key Infrastructure Project, Crustal Movement Observation Network of China. The velocity field delineates patterns of movements and deformation of active crustal blocks in continental China under the Eurasia-fixed reference frame. The space-based geodesy also clearly shows for the first time the horizontal movementand deformation of continental China induced by indentation of the Indian plate. The data provide kinematic constraints for simulating dynamic process of continental litho-spheric deformation. | MA Zongjin, CHEN Xinlian, YE Shuhua, LAI Xian, WEI Ziqing, CHEN Junrong, NING Jinsheng, XU Huoze & DING Guoyu1. Insitute of Geology, China Seismological Bureau, Beijing 100029, China (e-mail: disgroup@publi.bta.net.cn)2. Center for Analysis and Prediction, China Seismological Bureau, Beijing 100036, China 3. Shanghai Astronomic Observatory, Chinese Academy of Sciences, Shanghai 200030, China 4. Institute of Seismology, China Seismological Bureau, Wuhan 430077, China 5. Institute of Survey and Mapping, Xi’an 710054, China 6. National Bureau of Survey and Mapping, Beijing 100830, China 7. Wuhan University, Wuhan 430077, China 8. Institute of Geodesy and Geophysics, Wuhan 430077, China 9. China Seismological Bureau, Beijing 100036, China | 2001 | Chinese Science Bulletin2001，46，18： | 34 |
| 9 | Toll-like receptor 4/nuclear factor-kappa B signaling detected in brain after early subarachnoid hemorrhage显示文摘背景发炎和免疫在 subarachnoid 出血(SAH ) 以后在早大脑损害的致病起一个重要作用。原子 factor-kappa B (NF-KB ) 为发炎和免疫调整许多基因必需品并且被像使用费的受体(TLR ) 激活。这研究试图检测在早 SAH 以后在老鼠大脑发信号的像使用费的受体 4/nuclear factor-kappa B (TLR4/NF-KB ) 的表示。老鼠被将斩首的方法和他们的大脑在血的单个注射以后在 0, 2, 4, 6, 12, 24 和 48 个小时被移开进 prechiasmatic 水塘, TLR4 的 mRNA 表示被 immunohistochemistry 并且西方的弄污被 Taqman 即时 RT-PCR,和蛋白质表示测量。肿瘤坏死 factor-alpha (TNF-) 的 NF-KB 活动和集中, interleukin-lbeta (IL-) 和 intedeukin-6 (IL-6 ) 被试金(ELISA ).Results TaqMan 即时 RT-PCR 并且西方的弄污识别了的连接酶的 immunosorbent 测量在在 mRNA 和蛋白质的 TLR4 表示的一个 biphasic 变化:一座起始的山峰(2-6 小时) 和持续举起(12-48 小时) 。染色的 Immunohistochemical 在 glial 房间和脉管的内皮细胞层主要显示出 TLR4-1ike 免疫反应的可诱导的表示。在象 NF-KB-regulated proinflammatory cytokines (TNF- , IL-1 和 IL-6 ) 的生产一样的 NF-KB 子单元 p65 的激活的一个类似的 biphasic 变化被 ELISA 检测。结论这些数据建议试验性的 SAH 导致在早大脑损害发信号的 TLR4 表示和 NF-KB 的重要起来规定。发信号的 TLR4/NF-KB 的激活可以在 SAH 以后调整煽动性的回答。 | MA Chun-xiao YIN Wei-ning CAI Bo-wen WU Jian WANG Jun-yi HE Min SUN Hong DING Jun-li YOU Chao | 2009 | Chinese Medical Journal2009，，13： | 33 |
| 10 | Correction of β-thalassemia mutant by base editor in human embryos显示文摘 | Puping Liang Chenhui Ding Hongwei Sun Xiaowei Xie Yanwen Xu Xiya Zhang Ying Sun Yuanyan Xiong Wenbin Ma Yongxiang Liu Yali Wang Jianpei Fang Dan Liu Zhou Songyang Canquan Zhou Junjiu Huang | 2017 | Protein & Cell2017，8，11： | 34 |
| 11 | Removal characteristics of microplastics by Fe-based coagulants during drinking water treatment显示文摘Microplastics have caused great concern worldwide recently due to their ubiquitous presence within the marine environment. Up to now, most attention has been paid to their sources,distributions, measurement methods, and especially their eco-toxicological effects. With microplastics being increasingly detected in freshwater, it is urgently necessary to evaluate their behaviors during coagulation and ultrafiltration(UF) processes. Herein, the removal behavior of polyethylene(PE), which is easily suspended in water and is the main component of microplastics, was investigated with commonly used Fe-based salts. Results showed that although higher removal efficiency was induced for smaller PE particles, low PE removal efficiency(below 15%) was observed using the traditional coagulation process, and was little influenced by water characteristics. In comparison to solution pH, PAM addition played a more important role in increasing the removal efficiency, especially anionic PAM at high dosage(with efficiency up to 90.9%). The main reason was ascribed to the dense floc formation and high adsorption ability because of the positively charged Fe-based flocs under neutral conditions. For ultrafiltration, although PE particles could be completely rejected,slight membrane fouling was caused owing to their large particle size. The membrane flux decreased after coagulation; however, the membrane fouling was less severe than that induced by flocs alone due to the heterogeneous nature of the cake layer caused by PE, even at high dosages of Fe-based salts. Based on the behavior exhibited during coagulation and ultrafiltration, we believe these findings will have potential application in drinking water treatment. | Baiwen Ma Wenjing Xue Yanyan Ding Chengzhi Hu Huijuan Liu Jiuhui Qu | 2019 | Journal of Environmental Sciences2019，31，4： | 29 |
| 12 | Targeted migration of mesenchymal stem cells modified with CXCR4 to acute failing liver improves liver regeneration显示文摘AIM:To improve the colonization rate of transplanted mesenchymal stem cells(MSCs)in the liver and effect of MSC transplantation for acute liver failure(ALF).METHODS:MSC was modified with the chemokine CXC receptor 4(CXCR4)gene(CXCR4-MSC)or not(Null-MSC)through lentiviral transduction.The characteristics of CXCR4-MSCs and Null-MSCs were determined by real-time quantitative polymerase chain reaction,Western blotting and flow cytometry.CXCR4-MSCs and Null-MSCs were infused intravenously 24 h after administration of CCl4 in nude mice.The distribution of the MSCs,survival rates,liver function,hepatocyte regeneration and growth factors of the recipient micewere analyzed.RESULTS:In vitro,CXCR4-MSCs showed better migration capability toward stromal cell-derived factor-1αand a protective effect against thioacetamide in hepatocytes.In vivo imaging showed that CXCR4-MSCs migrated to the liver in larger numbers than Null-MSCs1 and 5 d after ALF.Higher colonization led to a longer lifetime and better liver function.Either CXCR4-MSCs or Null-MSCs exhibited a paracrine effect through secreting hepatocyte growth factor and vascular endothelial growth factor.Immunohistochemical analysis of Ki-67showed increased cell proliferation in the damaged liver of CXCR4-MSC-treated animals.CONCLUSION:Genetically modified MSCs expressing CXCR4 showed greater colonization and conferred better functional recovery in damaged liver. | Hu-Cheng Ma Xiao-Lei Shi Hao-Zhen Ren Xian-Wen Yuan Yi-Tao Ding | 2014 | World Journal of Gastroenterology2014，20，40： | 29 |
| 13 | Proteomic analysis of human ovaries from normal and polycystic ovarian syndrome显示文摘 | Ma,X Fan,L Meng,Y Hou,Z Mao,YDL Wang,W Ding,W Liu,JY | 2007 | 南京医科大学学报(自然科学版)2007，27，11： | 20 |
| 14 | Aortic Artery and Cardiac Valve Calcification are Associated with Mortality in Chinese Hemodialysis Patients: A 3.5 Years Follow-up显示文摘 | Xiao-Nong Chen Zi-Jin Chen Xiao-Bo Ma Bei Ding Hua-Wei Ling Zhong-Wei Shi Nan Chen | 2015 | Chinese Medical Journal2015，，20： | 20 |
| 15 | The poisoning and regeneration effect of alkali metals deposed over commercial V_2O_5-WO_3/TiO_2 catalysts on SCR of NO by NH_3显示文摘In this study,commercial V2O5-WO3/TiO2catalysts were deactivated by loading with alkali metals(K and Na).These catalysts were then regenerated by washing with either deionized water or 0.5 mol/L H2SO4(through the ultrasonic-assisted method).The samples used in this research were characterized by NH3-temperature programmed desorption(TPD),and X-ray photoelectron spectroscopy(XPS).Results showed that Na2O and K2O doping can poison the V2O5-WO3/TiO2catalyst and that the poisoning effect of Na2O was stronger than that of K2O.However,the Na2O-loaded sample was easier to regenerate than the K2O-loaded sample.The surfaces of catalysts can be sulfated by washing with dilute sulfuric acid because strong acid sites adhere to the catalyst surface.SO42-could also promote catalyst activity.As indicated by the NH3-TPD findings,the deposition of Na2O and K2O could also reduce the amount of desorbed ammonia and destabilize the acid sites,especially strong chemisorption sites.XPS results revealed that catalysts were deactivated by the decrease in the concentration of chemisorbed oxygen[the Oa/(Oα+Oβ)ratio].In the Na2O-doped catalyst,much chemisorbed oxygen was lost(from 28.8%to10.6%).However,the decrease in the Oa/(Oα+Oβ)ratio was less significant in the K2O-doped catalyst(from28.8%to 23.5%).Nonetheless,the binding energies of O1s broadened with respect to both high and low energy.In particular,the binding energy of chemisorbed oxygen increased from 531.5 to 531.8 eV. | Fengyu Gao Xiaolong Tang Honghong Yi Shunzheng Zhao Tongtong Zhang Dong Li Ding Ma | 2014 | Chinese Science Bulletin2014，59，31： | 19 |
| 16 | 基于轮廓特征及灰度相似度匹配的接触网绝缘子缺陷检测(英文)显示文摘目的:在图像缺陷样本少和一致性差的情况下,实现精确可靠的接触网绝缘子缺陷检测。创新点:提山一种基于瓷片轮廓特征及灰度相似度匹配的融合算法,实现了绝缘子瓷片的轮廓提取及绝缘子各瓷片的精准分离,并构建了基于瓷片间距和灰度相似度匹配的绝缘子缺陷检测模型。方法:1.采用同一个绝缘子相邻瓷片两两比较的方法进行缺陷检测,解决图像缺陷样本少和一致性差的问题。2.分两步进行检测(Fig.2):(1)基于水平梯度特征提取绝缘子各瓷片轮廓,并对瓷片轮廓内像素进行复原;(2)计算瓷片间距和灰度相似度,并与设置的阈值进行比较以区分正常绝缘子和缺陷绝缘子。结论:1.实验表明,基于轮廓特征及灰度相似度匹配的方法能够有效区分正常绝缘子和缺陷绝缘子。2.在图片数据集中,测试达到了99.50%的高召回率和91.71%的高精确度,满足了目前较高水平的接触网绝缘子缺陷检测的要求。 | Ping TAN Xu-feng LI Jin-mei XU Ji-en MA Fei-jie WANG Jin DING You-tong FANG Yong NING | 2020 | Journal of Zhejiang University-Science A(Applied Physics & Engineering)2020，21，1： | 19 |
| 17 | Value of gamma-glutamyltranspeptidase-to-platelet ratio in diagnosis of hepatic fibrosis in patients with chronic hepatitis B显示文摘AIM To investigate the value of the gamma-glutamyltraspeptidase(GGT)-to-platelet(PLT) ratio(GPR) in the diagnosis of hepatic fibrosis in patients with chronic hepatitis B(CHB). METHODS We included 390 untreated CHB patients in this study. The GPR, aspartate aminotransferase(AST)-to-PLT ratio index(APRI), and fibrosis-4(FIB-4) of all patients were analysed to determine if these parameter were correlated with age, gender, medical history, liver function [total bilirubin(TBil), alanine aminotransferase(ALT), and AST], GGT, PLT count, or hepatic fibrosis stage. The GPR, APRI, and FIB-4, as well as the combination of the GPR and APRI or the GPR and FIB-4 were assessed in different cirrhosis stages using receiver operating characteristic(ROC) curve analysis to evaluate their value in diagnosing hepatic fibrosis in CHB patients. RESULTS The GPR, APRI, and FIB-4 were not correlated withCHB patients' age, gender, or disease duration(P > 0.05), but all of these parameters were positively correlated with serum ALT, AST, GGT, and PLT count(P < 0.01). Additionally, the GPR, APRI, and FIB-4 were positively correlated with hepatic fibrosis(P < 0.01); the areas under the ROC curve for the GPR in F1, F2, F3, and F4 stages were 0.723, 0.741, 0.826, and 0.833, respectively, which were significantly higher than the respective values for the FIB-4 and APRI(F1: 0.581, 0.612; F2: 0.706, 0.711; F3: 0.73, 0.751; and F4: 0.799, 0.778). The respective diagnostic cut-off points for each stage were 0.402, 0.448, 0.548, and 0.833, respectively. The diagnostic sensitivity and specificity were, respectively, 88.8% and 87.5% in F1, 72.7% and 89.7% in F2, 81.3% and 98.6% in F3, and 80% and 97.4% in F4 when the GPR and APRI were connected in parallel; 86.6% and 90.2%, 78.4% and 96%, 78.6% and 97.4%, and 73.2% and 97.9%, respectively, when the GPR and APRI were connected in series; 80.2% and 89%, 65% and 89%, 70.3% and 98.5%, and 78.8% and 96.8%, respectively, when the GPR and FIB-4 were connected in parallel; and 83.6% and 87.9%, 76.8% and 96.6%, 72.7% and 98%, and 74.4% and 97.7%, respectively, when the GPR and FIB-4 were connected in series.CONCLUSION The GPR, as a serum diagnostic index of liver fibrosis, is more accurate, sensitive, and easy to use than the FIB-4 and APRI, and the GPR can significantly improve the sensitivity and specificity of hepatic fibrosis diagnosis in CHB when combined with the FIB-4 or APRI. | Yan-Chao Hu Hao Liu Xiao-Yan Liu Li-Na Ma Yu-Hua Guan Xia Luo Xiang-Chun Ding | 2017 | World Journal of Gastroenterology2017，23，41： | 19 |
| 18 | Incidence of infectious complications is associated with a high mortality in patients with hepatitis B virus-related acute-on-chronic liver failure显示文摘BACKGROUND In China,hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF) is the most common liver failure characterized by serious clinical syndromes of liver decompensation with a very high mortality.Bacterial and/or fungal infections are the most common complications that are associated with high short-term mortality.Bacterial translocation from the intestine,impaired hepatic clearance,and immune paralysis of circulating immune cells are thought to contribute to infectious complications in liver failure.The control of bacterial and fungal infections is the key to improving HBV-ACLF outcomes.Active prevention,early diagnosis,and timely treatment of bacterial and fungal infections are essential for treating HBV-ACLF.AIM To investigate the frequency and role of bacterial and fungal infections in patients with HBV-ACLF.METHODS Patients with HBV-ACLF hospitalized at Taihe Hospital,Hubei University of Medicine from January 2014 to December 2017 were retrospectively enrolled.Patient-related information was retrieved from the hospital case database,including general information,blood biochemistry,complications,etc.According to the occurrence of secondary infection or not,the patients were divided into an infection group and a non-infection group.The sites,types,and incidences of bacterial and fungal infections and the influence of infections on the prognosis of HBV-ACLF were statistically analyzed.The risk factors for infections were assessed by unconditional logistic regression.RESULTS There were 174 cases of HBV-ACLF that met the enrollment criteria,of which 114 (65.52%) were diagnosed with infectious complications.Infections occurred in the abdominal cavity (87 cases),respiratory tract (51 cases),urinary tract (18 cases),and biliary tract (10 cases).Patients with infectious complications had a significantly higher 28-d mortality (70.18%,80/114) than those without (40.00%,24/60)(70.18% vs 40.00%,P < 0.05).And patients with infectious complications had a much higher incidence of non-infectious complications (54.39%,62/114)(54.39% vs 15.00%,P < 0.05),leading to an extremely high 28-d mortality of 88.71%(55/62)(P < 0.05).The grade of liver failure,period of hospital stay ≥ 30 d,age ≥ 45 years,and percentage of neutrophils > 70% were identified as risk factors for infectious complications.CONCLUSION The high incidence of infectious complications in patients with HBV-ACLF is associated with severity and deterioration of the disease and may contribute to the extremely high mortality of these patients. | Chen Wang De-Qiang Ma Sen Luo Chuan-Min Wang De-Ping Ding You-You Tian Kang-Jian Ao Yin-Hua Zhang Yue Chen Zhong-Ji Meng | 2019 | World Journal of Clinical Cases2019，7，16： | 18 |
| 19 | A general end-to-end diagnosis framework for manufacturing systems显示文摘The manufacturing sector is envisioned to be heavily influenced by artificial-intelligence-based technologies with the extraordinary increases in computational power and data volumes. A central challenge in the manufacturing sector lies in the requirement of a general framework to ensure satisfied diagnosis and monitoring performances in different manufacturing applications. Here, we propose a general data-driven,end-to-end framework for the monitoring of manufacturing systems. This framework, derived from deep-learning techniques, evaluates fused sensory measurements to detect and even predict faults and wearing conditions. This work exploits the predictive power of deep learning to automatically extract hidden degradation features from noisy, time-course data. We have experimented the proposed framework on 10 representative data sets drawn from a wide variety of manufacturing applications. Results reveal that the framework performs well in examined benchmark applications and can be applied in diverse contexts,indicating its potential use as a critical cornerstone in smart manufacturing. | Ye Yuan Guijun Ma Cheng Cheng Beitong Zhou Huan Zhao Hai-Tao Zhang Han Ding | 2020 | National Science Review2020，7，2： | 18 |
| 20 | Comprehensive metabolomics expands precision medicine for triple-negative breast cancer显示文摘Metabolic reprogramming is a hallmark of cancer.However,systematic characterizations of metabolites in triple-negative breast cancer(TNBC)are still lacking.Our study profiled the polar metabolome and lipidome in 330 TNBC samples and 149 paired normal breast tissues to construct a large metabolomic atlas of TNBC.Combining with previously established transcriptomic and genomic data of the same cohort,we conducted a comprehensive analysis linking TNBC metabolome to genomics.Our study classified TNBCs into three distinct metabolomic subgroups:C1,characterized by the enrichment of ceramides and fatty acids;C2,featured with the upregulation of metabolites related to oxidation reaction and glycosyl transfer;and C3,having the lowest level of metabolic dysregulation.Based on this newly developed metabolomic dataset,we refined previous TNBC transcriptomic subtypes and identified some crucial subtype-specific metabolites as potential therapeutic targets.The transcriptomic luminal androgen receptor(LAR)subtype overlapped with metabolomic C1 subtype.Experiments on patient-derived organoid and xenograft models indicate that targeting sphingosine-1-phosphate(S1P),an intermediate of the ceramide pathway,is a promising therapy for LAR tumors.Moreover,the transcriptomic basal-like immune-suppressed(BLIS)subtype contained two prognostic metabolomic subgroups(C2 and C3),which could be distinguished through machine-learning methods.We show that N-acetyl-aspartyl-glutamate is a crucial tumor-promoting metabolite and potential therapeutic target for high-risk BLIS tumors.Together,our study reveals the clinical significance of TNBC metabolomics,which can not only optimize the transcriptomic subtyping system,but also suggest novel therapeutic targets.This metabolomic dataset can serve as a useful public resource to promote precision treatment of TNBC. | Yi Xiao Ding Ma Yun-Song Yang Fan Yang Jia-Han Ding Yue Gong Lin Jiang Li-Ping Ge Song-Yang Wu Qiang Yu Qing Zhang François Bertucci Qiuzhuang Sun Xin Hu Da-Qiang Li Zhi-Ming Shao Yi-Zhou Jiang | 2022 | Cell Research2022，32，5： | 18 |
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