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| 1 | Quantitative analysis of hepatoma-specific α-fetoprotein(HS-AFP) by a new mini-column affinity chromatography and its clinical value in diagnosis of hepatocellular carcinoma显示文摘Objective:To establish a convenient and economic method to determine hepatoma-specific α-fetoprotein(HS-AFP) for diagnosis of hepatocellular carcinoma(HCC). Methods:HS-AFP from serum of HCC patients was separated by a mini-column Lens culinaris agglutinin(LCA)-affinity chromatography. The levels of serum total AFP and separated HS-AFP were detected by radioimmunoassay(RIA). Results:Circulating AFP was separated into three peaks(AFP-1,AFP-2,and AFP-3) by LCA-affinity chromatography. During the elution course,the AFP-1 and AFP-2 could be eluted with TE buffer. HS-AFP(AFP-3) from sera of HCC patients was eluted clearly on the LCA-sepharose gel mini-column with a solution containing α-methyl-D-mannoside. It was a part of total AFP and only found in sera of HCC patients. A ratio of more than 15% for HS-AFP to total AFP in serum was considered as a specific marker for HCC diagnosis with higher sensitivity(92.7%) and specificity(88.2%). Conclusion:The new assay for circulating HS-AFP analysis is more sensitive,repeatable,and convenient. Its clini-cal application would be useful to early diagnosis of HCC. | Wei Wu Dengfu Yao Liwei Qiu Xiaoxiao Gu Xinhua Wu | 2008 | The Chinese-German Journal of Clinical Oncology2008，7，3： | 4 |
| 2 | Preparation and characterization of porous titanium using space-holder technique显示文摘基于钛的多孔的材料能在结构的应用被使用并且医药因为他们的优秀机械性质,在提高的温度,好腐蚀抵抗和奇妙的简历相容性植入。然而,大多数过去常生产多孔的金属罐头的方法仅仅给有限的孔和控制不了的毛孔形态学。在现在的学习,用空间持有者技术的粉末冶金学的一个最新发达的方法被用来与可控制的孔制作多孔的钛。词法特征和产品的机械性质充分被调查。结果证明孔在范围 55%75% ,并且与 ∼0.72 的平均球状,吝啬的毛孔尺寸是 600 μm。高原压力在 10 MPa 和 35 MPa 之间变化。是由 Gibson-Ashby 模型,预言了高原压力与增加减少孔。 | NIU Wenjuan BAI Chenguang QIU Guibao WANG Qiang WEN Liangying CHEN Dengfu DONG Lingyan | 2009 | Rare Metals2009，28，4： | 3 |
| 3 | Abnormal expression of vascular endothelial growth factor (VEGF) and its clinical features in tissues of human lung cancer显示文摘Objective: Angiogennesis, the formation of new blood vessels from the existing vascular bed, is essential step for growth and invasion of primary tumor. Vascular endothelial growth factor (VEGF) is known to play crucial role in tumor angiogenesis. In the present study, we investigate the expression of VEGF and VEGF-mRNA in the angiogennesis, metastasis and prognosis of lung cancer. Methods: The VEGF cellular distributions and expression in 38 specimens of patients with lung cancer were investigated with immunohistochemistry stain technology. The total RNAs in 38 tissues of lung cancer was measured, then the levels of VEGF-mRNA expression were analyzed by a reverse-transcription polymerase chain reaction (RT-PCR) assay. The levels of VEGF in sera of patients with lung cancer, benign lung diseases and healthy controls were detected through Enzyme linked immunosorbent assay (ELISA) method. Results: The VEGF positive stain was 76% in 38 cases of lung cancer specimens. The 89% rate of VEGF stain was found for clinical stage Ⅲ cases and 92% for stage Ⅳ lung cancers. The significantly higher expression of VEGF was evidenced in patients with lymph node metastasis (84%), distant metastasis (90%), and lung cancers with lower histological differentiation (89%), respectively. The expression level of total RNA was significantly higher in patients with lung cancers than that in their paracancerous or distant lung tissues. The VEGF expressions were tightly correlated with total RNA concentration of lung carcinoma (P<0.01). The predominant expressions of VEGF121 and VEGF165 gene fragments were found in lung cancer specimens by RT-PCR analysis. No significant difference of serum VEGF levels was detected between cases with lung cancer and patients with benign diseases. However, the VEGF level of cases with benign diseases was decreased significantly after patients with anti-inflammation medication. Conclusion: The present data suggested that the tumor tissue VEGF expression and VEGF-mRNA analysis in patients with lung cancer be a useful indicator for angiogenesis and metastasis of lung cancer. | Xinhua Wu Dengfu Yao Gongshen Shi Liwei Qiu Wei Wu Songshi Ni Xueguang Zhang | 2005 | Journal of Nanjing Medical University2005，19，4： | 3 |
| 4 | Quantitative effects of phase transition on solute partition coefficient,inclusion precipitation, and microsegregation for high-sulfur steel solidification显示文摘Segregation and inclusion precipitation are the common behaviours of steel solidification, which are resulted from the redistribution and diffusion of the solute elements at the solid-liquid interface. The effect of the phase transition of high-sulfur free-cutting steel is quantified in the present work for the solute partition coefficient(ki), inclusion precipitation, and microsegregation by establishing a coupling model of microsegregation and inclusion precipitation, wherein the quantified dependencies of ki in terms of temperature, phase and carbon(C) content were applied. Results showed that the solidification temperature range and phase transition of high-sulfur steel that under different solidification paths and C contents were quite different, leading to differences in ki and eventually in microsegregation. kC,kP, and kS were mainly affected by phase composition and kSi was primarily by temperature, while kMn depended on both phase composition and temperature during solidification. Increasing the C content within the interval 0.07-0.48 wt%, the ‘proportion of the δ phase maintained temperature region during solidification’(Pδ), kave Pand kave S(kiave, the average value of the ki across the whole stages of solidification)decreased monotonically, whereas kave Cincreased linearly. The peritectic reaction impacted on the phase composition and ki, leading to the change in microsegregation. Such effect of the peritectic reaction was more significant at the last stage of solidification. When the Pδ was between 75% and 100%(corresponding to 0.07-0.16 wt% C), the solidification path resulted in a greater effect on the microsegregation of solutes C, P, and S because of the peritectic reaction. The microsegregation of solutes Mn and S were comprehensively influenced by kMn, kS and Mn S precipitation as well. The studies would help reveal the solute redistribution at the solid-liquid interface, and improve the segregation of high-sulfur steel by controlling the solidification and precipitation in practice. | Lintao Gui Mujun Long Shixin Wu Zhihua Dong Dengfu Chen Yunwei Huang Huamei Duan Levente Vitos | 2019 | Journal of Materials Science & Technology2019，35，10： | 3 |
| 5 | Circulating specific biomarkers in diagnosis of hepatocellular carcinoma and its metastasis monitoring显示文摘 | Li Wang Min Yao Zhizhen Dong Yun Zhang Dengfu Yao | 2014 | Tumor Biology2014，，1： | 2 |
| 6 | Preparation of low cost glass–ceramics from molten blast furnace slag显示文摘 | Yan Zhao Dengfu Chen Yanyan Bi Mujun Long | 2011 | Ceramics International2011，，3： | 2 |
| 7 | Encouraging microRNA-based Therapeutic Strategies for Hepatocellular Carcinoma显示文摘 | Min Yao Li Wang Liwei Qiu Qi Qian Dengfu Yao | 2015 | Anti-Cancer Agents in Medicinal Chemistry2015，，4： | 2 |
| 8 | Friction and wear properties of mag-netron sputtered DLC/SiC films on magnesium alloy显示文摘 | Xu Xiaojing Xia Dengfu | 2009 | Mate-rials Science Forum2009，61，0611： | 1 |
| 9 | Abnormal expression of VEGF and its gene transcription status as diagnostic indicators in patients with non-small cell lung cancer显示文摘Objective Angiogenesis is known to be essential for the survival,growth,invasion,and metastasis of lung cancer cells. Vascular endothelial growth factor(VEGF) is an important factor regulating angiogenesis of non-small cell lung cancer(NSCLC); however,its pathologic features and significance are unclear. In this study,the tissue VEGF expression levels and its gene transcriptional status,as well as circulating VEGF levels,were investigated in patients with lung disease. Methods VEGF protein and m RNA expression levels in 38 lung tissue samples were analyzed by immunohistochemistry and reverse transcription-polymerase chain reaction(RT-PCR),respectively. Circulating VEGF levels were detected quantitatively by an enzyme linked immuno-sorbent assay. Results The level of VEGF expression was significantly higher in lung cancer tissue than in the corresponding paracancerous or non-cancerous tissues. The average level of VEGF-positive staining was 76% in tissue samples from NSCLC patients; the levels were 89% in tissue samples from stage III patients and 92% in stage IV patients. High VEGF expression was also evident in cases with lymph node metastasis(84%),distant metastasis(90%),and lower differentiation degree(89%). VEGF m RNA in cancerous tissues was represented predominantly by the VEGF121 and VEGF165 isoforms. Circulating VEGF levels were significantly higher in NSCLC patients [(840 ± 324) pg/m L] than in patients with benign lung diseases [(308 ± 96) pg/m L] or in healthy individuals serving as controls [(252 ± 108) pg/m L]. Conclusion The over-expression of lung VEGF and its gene transcription status should be useful molecular indicators for NSCLC diagnosis. | Yun Shi Yang Shi Xuli Yang Jianrong Chen Qi Qian Dengfu Yao Guangzhou Wu | 2015 | Oncology and Translational Medicine2015，1，5： | 1 |
| 10 | The calculation of section of A1203-CaO-SiO2 system and its verifieation显示文摘 | DONG Lingyan CHEN Dengfu BAI Chenguang | 2004 | Journal of Chongqing University: Natural Science Edition2004，27，1： | 1 |
| 11 | Orifice Flow Charateristics 显示文摘 | CHEOLWAN Kim DENGFU CHIM-Yuan PERNG ZHANG Dengfu | 2003 | Ford Technical Journal2003，6，3： | 1 |
| 12 | Clinicopathological features of hypoxia-inducible factor-1α and vascular endothelial growth factor expression in patients with lung cancer显示文摘Objective The aim of the study was to investigate the clinicopathological characteristics of hypoxiainducible factor-1α(HIF-1α) and vascular endothelial growth factor(VEGF) expression in patients with lung cancer.Methods Cancerous and noncancerous tissues were collected post-operation from 115 patients with lung cancers by the self-control method. Total RNA was extracted from the lung tissues. The status of tissue HIF-1α expression and intercellular distribution was observed by immunochemistry using a tissue microarray. The expression levels of circulating HIF-1α and VEGF were detected by enzyme-linked immunosorbent assay(ELISA).Results The expression of serum HIF-1α [(138.3 ± 28.8) μg/L] in the group of patients with lung cancer was significantly higher(P < 0.01) than that in the group of patients with pneumonia [(58.8 ± 14.5) μg/L] and the control group of patients ((24.1 ± 3.3) μg/L)There was a strong positive correlation of serum HIF-1α levels(r = 0.937, P < 0.01) with serum VEGF levels. The specific concentration of total RNA [(1.52 ± 1.14) μg/mg wet lung tissues] in the cancerous tissues was significantly higher(t = 8.494, P < 0.001) than that in the noncancerous tissues ((0.58 ± 0.33) μg/mg)The clinicopathological features of HIF-1α expression in lung cancer tissues revealed a significant relationship between positive HIF-1α expression and patient sex(χ~2 = 4.494, P = 0.034), tumor size(χ~2 = 4.679, P = 0.031), differentiation degree(χ~2= 8.846, P = 0.012), and presence of lymphatic node metastasis(χ~2= 6.604, P = 0.037).Conclusion Abnormal HIF-1α expression in lung cancer is closely related with nucleic acid metabolism and angiogenesis, and it may be helpful in the diagnosis and identification of lung cancer. | Xuli Yang Li Wang Wenli Sai Yin Cai Juanjuan Gu Xin Chen Dengfu Yao | 2016 | Oncology and Translational Medicine2016，2，6： | 1 |
| 13 | A new method for calculating the activity of stable compound from binary phase diagram显示文摘A new method to calculate the activity of a stable compound in a binary phase diagram was presented and dis- cussed. According to the formula for calculating activity from the binary phase diagram, the equilibrium constant can be calculated through the mass action principle after the activities of two pure components were computed respectively. Based on that, the activity of a stable compound can be easily obtained at last. The activity of the stable compound InSb is calcu- lated in the In-Sb binary system by using this method. The result is well consistent with another calculation value. | CHEN Dengfu DONG Lingyan BAI Chenguang LIU Qingcai WANG Chuanjun | 2006 | Rare Metals2006，25，5： | 1 |
| 14 | Proparation of low cost glass-ceramics from molten blast furnace slag显示文摘 | Zhao Yan Chen Dengfu Bi Yanyan et a/ | 2012 | Ceramics Inter- national2012，38，3： | 1 |
| 15 | Experimental study of gamma glutamyltranspeptidase isoforms in early diagnosis of hepatoma显示文摘 | Chen Shuzhou Yao Dengfu Huang Jiefei | 1996 | Clin J liver1996，1，3： | 1 |
| 16 | Compen- sation control model of superheat and cooling water temper- ature for secondary cooling of continuous casting显示文摘 | Zhang Jian Chen Dengfu Wang Shuigen | 2011 | Steel Research Int2011，82，3： | 1 |
| 17 | 循环血AFP-mRNA与HS-GGT检测对肝癌的诊断与监测价值(英文)显示文摘Objective: Early finding of hepatocellular carcinoma(HCC) and monitoring of its metastasis are of the utmost importance. The objectives of this study were to investigate the values of circulating hepatoma-specific gamma-glutamyl transferase(HS-GGT) fraction and α-fetoprotein mRNA(AFP-mRNA) from peripheral blood mononuclear cells(PBMCs) in diagnosis or monitoring metastasis of HCC. Methods: Total RNA was extracted from hepatomas or PBMCs of patients, synthesized to AFP-cDNA through random primers and reverse transcriptase, amplified by using a nested PCR assay, and confirmed by sequencing. Simultaneity, the HS-GGT activities were quantitatively determined in sera of patients. The comprehensive assessments of two markers were investigated in HCC patients. Results: The amplified fragments of AFP-mRNA were identical to original designed ones(159 bp) and confirmed by sequencing. The lowest sensitivity was 2 ng/L of total RNA. The incidences of AFP-mRNA and HS-GGT were 60.4%(113 of 187) and 84.5%(158 of 187) in the HCC group, and their significantly higher(P < 0.01) than that in any of other groups. The high frequencies of HS-GGT and AFP-mRNA were 78.2% and 56.4% in the HCC patients with AFP < 50 ng/mL. All positive of AFP-mRNA was found in HCC patients with extrahepatic metastasis. No significant correlation was found between tumor size and AFP-mRNA or HS-GGT. The total incidence of combined AFP-mRNA and HS-GGT detection was 93.6 % for HCC diagnosis. Conclusion: HS-GGT and AFP-mRNA are specific markers for HCC diagnosis or differentiation, especially the former for early finding and the later for monitoring metastasis of HCC. | Li Wang Min Yao Yao Yao Liuhong Pan Shaolin Lu Dengfu Yao | 2014 | The Chinese-German Journal of Clinical Oncology2014，13，10： | 0 |
| 18 | 肝癌缺氧诱导因子-lα异常表达与靶向干预的临床价值研究(英文)显示文摘Objective:The aim of this study was to analyze the expression features of hypoxia inducible factor-1α (HIF-1α) in hepatocellular carcinoma (HCC) and effects of HIF-1α silencing on HepG2 cells.Methods:HIF-1α expression was analyzed in the self-control HCC specimens by immunohistochemistry.After HepG2 cells with miRNA transfection,the expression of HIF-1α was determined at mRNA or protein level by real-time polymerase chain reaction (PCR) or Western blotting.Vascular endothelial growth factor (VEGF) and angiopoietin-2 (ANG-2) were determined by ELISA.Alterations of cell cycles and apoptosis of HepG2 cells were measured using a flow cytometer.Results:Positive HIF-1α was brown and granule-like in the cytoplasm or nucleus.Significant difference was found between HCC (80%) and its surrounding tissues (100%,χ2=22.35,P < 0.001) and HIF-1α expression related to tumor size.At 72 h after miRNA transfection,the expression of HIF-1α in HepG2 cells was down-regulated by 87% at mRNA or 65% at protein level,with VEGF and ANG-2 decreased to 54% and 36%,respectively.After RNA interference combined with anti-cancer drug,the apoptotic rate of HepG2 cells was increasing from 22.46% ± 0.61% to 36.99% ± 0.88%,with up-regulation of G1 phase (65.68% ± 0.91%) and down-regulation of S phase (19.47 ± 1.34 %).Conclusion:Abnormal expression of HIF-1α is associated with development of HCC,and HIF-1α gene silencing can effectively inhibit HepG2 cell proliferation. | Shanshan Li Dengfu Yao Zhizhen Dong Yajie Qian Dandan Yu Ninghua Yao Jie Chen Xiaodi Yan Chenglin Qin | 2012 | The Chinese-German Journal of Clinical Oncology2012，11，3： | 0 |
| 19 | Influences of fisheries management measures on biological characteristics of threadfin bream(Nemipterus virgatus)in the Beibu Gulf,South China Sea显示文摘Long-term variations in population structure,growth,mortality,length at median sexual maturity,and exploitation rate of threadfin bream(Nemipterus virgatus)are reported based on bottom trawl survey data collected during 1960-2012 in the Beibu Gulf,South China Sea.Laboratory-based analyses were conducted on 16791 individuals collected quarterly in eight different sampling years.Average body length,estimated asymptotic length,and percentage of large individuals have decreased significantly with the growth of marine catch and fishing power,indicating individual miniaturization of this fish species.Estimated exploitation rates indicate that the N.virgatus stock in the Beibu Gulf was moderately exploited in 1960 and 1962 and overexploited after 1992.This stock was taking a good turn in status in 2012,with the lowest exploitation rate since 1992 and ceased downward trend in length indexes.These results suggest that management measures to reduce fishing pressure may have a positive influence on the biological characteristics of this commercial fish species.Biological characteristics of most commercial fish species have phenotypic plasticity and might change over years in response to fisheries management.Therefore,attentions should be paid on variations in fish biological characteristics,when evaluating the effectiveness of current measures to control the total catch for all fisheries. | Kui Zhang Ping Geng Jiajun Li Youwei Xu Muhsan Ali Kalhoro Mingshuai Sun Dengfu Shi Zuozhi Chen | 2022 | Acta Oceanologica Sinica2022，41，3： | 0 |
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