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| 1 | Expression of p53,Bax and Bcl-2 proteins in hepatocytes in non-alcoholic fatty liver disease显示文摘AIM: To analyze the protein expression essential for apoptosis in liver steatosis. METHODS: The expression of proapoptotic proteins p53, Bax, and antiapoptotic Bcl-2 in hepatocytes with steatosis (SH) and without steatosis (NSH) was evaluated in 84 patients at various stages of non-alcoholic fatty liver disease (NAFLD). RESULTS: Immunohistochemical staining of liver tissue showed the activation of p53 protein in SH and NSH with increased liver steatosis, diminished Bcl-2 and slightly decreased Bax protein. Positive correlation was found between the stage of liver steatosis with p53 expression in SH (r = 0.54, P < 0.01) and NSH (r = 0.49, P < 0.01). The antiapoptotic protein Bcl-2 was diminished together with the advancement of liver steatosis, especially in non-steatosed hepatocytes (r =0.43, P < 001). CONCLUSION: Apoptosis is one of the most important mechanisms leading to hepatocyte elimination in NAFLD. The intensification of inflammation in NAFLD induces proapoptotic protein p53 with the inhibition of antiapoptotic Bcl-2. | Anatol Panasiuk Janusz Dzieciol Bozena Panasiuk Danuta Prokopowicz | 2006 | World Journal of Gastroenterology2006，12，38： | 46 |
| 2 | Plasma matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 as biomarkers of ulcerative colitis activity显示文摘AIM: Overexpression of mucosal metalloproteinases (MMP)has been demonstrated recently in inflammatory boweldisease. Their activity can be counterbalanced by the tissueinhibitor of metalloproteinases (TIMP). The aim of this studywas to evaluate the effect of ulcerative colitis (UC) on MMP-1 and TTMP-1 plasma concentrations, as two possiblebiomarkers of the disease activity.METHODS: MMP-1 and TIMP-1 plasma concentrations weremeasured with an enzyme immunoassay in 16 patients withendoscopically confirmed active UC.RESULTS: Plasma concentrations of both MMP-11 (13.7±0.2ng/ml) and TIMP-L (799±140 ng/ml) were significantlyelevated in UC patients in comparison to healthy controls(11.9±0.9 ng/ml and 220±7 ng/ml respectively). There wasno correlation between TIMP-1 and MMP-1 concentrations(r=-0.02). TIMP-1 levels revealed significant positivecorrelations with scored endoscopic degree of mucosai injury,disease activity index and clinical activity index values aswell as C-reactive protein concentration. There was nocorrelation between MMP-1 and laboratory, clinical orendoscopic indices of the disease activity.CONCLUSION: These results confirm the role of both MMP-1 and TIMP-1 in the pathogenesis of ulcerative colitis.However only TIMP-1 can be useful as a biomarker of thedisease activity, demonstrating association with clinical andendoscopic pictures. | Alicja Wiercinska-Drapalo Jerzy Jaroszewicz Robert Flisiak Danuta Prokopowicz | 2003 | World Journal of Gastroenterology2003，9，12： | 21 |
| 3 | Diet and nutritional factors in inflammatory bowel diseases显示文摘Inflammatory bowel disease(IBD) development is affected by complex interactions between environmental factors, changes in intestinal flora, various predisposing genetic properties and changes in the immune system. Dietary factors seem to play an underestimated role in the etiopathogenesis and course of the disease. However, research about food and IBD is conflicting. An excessive consumption of sugar, animal fat and linoleic acid is considered a risk factor for IBD development, whereas a high fiber diet and citrus fruit consumption may play a protective role. Also, appropriate nutrition in particular periods of the disease may facilitate achieving or prolonging remissions and most of all, improve the quality of life for patients. During disease exacerbation, a low fiber diet is recommended for most patients. In the remission time, an excessive consumption of alcohol and sulfur products may have a negative effect on the disease course. Attempts are also made at employing diets composed in detail in order to supplement IBD therapy. A diet with a modified carbohydrate composition, a semi-vegetarian diet and a diet low in fermentable oligosaccharides, disaccharides, monosaccharides and polyols are under investigation. Due to chronic inflammation as well as side effects of chronically used medications, patients with IBD are also at increased risk of nutritional factor deficiencies, including iron, calcium, vitamin D, vitamin B12, folic acid, zinc, magnesium and vitamin A. It should also be remembered that there is no single common diet suitable for all IBD patients; each of them is unique and dietary recommendations must be individually developed for each patient, depending on the course of the disease, past surgical procedures and type of pharmacotherapy. | Danuta Owczarek Tomasz Rodacki Renata Domagała-Rodacka Dorota Cibor Tomasz Mach | 2016 | World Journal of Gastroenterology2016，22，3： | 19 |
| 4 | Plasma interleukin-18 reflects severity of ulcerative colitis显示文摘AIM: The aim of this study was to evaluate the association between ulcerative colitis activity and plasma or mucosal concentrations of interleukin (IL)-18.METHODS:Il-18 concentrations were measured in plasma and mucosal samples from 15 patients with active ulcerative colitis (UC).RESULTS: The mean plasma concentration of IL-18 measured in all patients (422±88 pg/mL) doubled the mean value in healthy controls (206±32 pg/mL); however,the difference was not statistically significant. Plasma IL-18 levels revealed a significant positive correlation with scored endoscopic degree of mucosal injury, disease activity index, clinical activity index and C-reactive protein concentration. The mean concentration of plasma IL-18 was significantly higher in patients with severe ulcerative colitis (535±115 pg/mL) than in patients with mild ulcerative colitis (195±41 pg/mL), and in healthy controls.Although the mucosal mean IL-18 concentration in severe ulcerative colitis (2 523±618 pg/mg protein) doubled values observed in mild one (1 347±308 pg/mg protein), there was no statistically significant difference.CONCLUSION: Plasma IL-18 can be considered as a surrogate marker helpful in evaluation of ulcerative colitis activity. | Alicja Wiercinska-Drapalo Flobert Flisiak Jerzy Jaroszewicz Danuta Prokopowicz | 2005 | World Journal of Gastroenterology2005，11，4： | 16 |
| 5 | Inflammatory bowel disease:Epidemiology,pathology and risk factors for hypercoagulability显示文摘Hypercoagulability observed in patients with inflammatory bowel diseases(IBD)may lead to thromboembolic events(TE),which affect the venous and arterial systems alike and are an important factor in patients’morbidity and mortality.The risk of TE in IBD patients has been demonstrated to be approximately threefold higher as compared to the general population.The pathogenesis of thrombosis in IBD patients is multifactorial and not fully explained.The most commonly listed factors include genetic and immune abnormalities,disequilibrium between procoagulant and anticoagulant factors,although recently,the role of endothelial damage as an IBD-triggering factor is underlined.Several studies report that the levels of some coagulation enzymes,including fibrinogen,factorsⅤ,Ⅶ,Ⅷ,active factorⅪ,tissue factor,prothrombin fragment 1+2and the thrombin-antithrombin complex,are altered in IBD patients.It has been demonstrated that there is a significant decrease of tissue plasminogen activator level,a marked increase of plasminogen activator inhibitor type 1 and thrombin-activable fibrinolysis inhibitor,a significantly lower level of antithrombinⅢand tissue factor pathway inhibitor.IBD patients have been also observed to produce an increased amount of various anticoagulant antibodies.Hyperhomocysteinemia,which is a potential risk factor for TE was also observed in some IBD patients.Further studies are necessary to assess the role of coagulation abnormalities in IBD etiology and to determine indications for thromboprophylactic treatment in patients at high risk of developing TE. | Danuta Owczarek Dorota Cibor Mikolaj K Glowacki Tomasz Rodacki Tomasz Mach | 2014 | World Journal of Gastroenterology2014，20，1： | 16 |
| 6 | High concentration of antimitochondrial antibodies predicts progressive primary biliary cirrhosis显示文摘AIM: To evaluate the serum concentration of antimitochondrial antibodies (AMAs) as a prognostic indicator of progressive primary biliary cirrhosis (pPBC).METHODS: Serum concentrations of AMA subtypes (anti-M2, anti-M4, and anti-M9), biochemical indices of liver function and Mayo risk factor (MRF) were determined in 30 women with diagnosed primary biliary cirrhosis (PBC) selected among 348 females with elevated alkaline phosphatase but without signs of hepatic decompensation.They were followed up for 5 years for possible development of hepatic decompensation.RESULTS: Anti-M2 concentration was significantly correlated with bilirubin and albumin levels as well as MRF, whereas anti-M4 was significantly correlated with albumin level, prothrombin time and MRF. During the 5-year follow-up, progressive PBC (pPBC) was diagnosed in 3 among 23 patients available for evaluation. These 3patients were positive for both anti-M2 and anti-M4.Anti-M2 serum concentration exceeded 1 300 RU/mL in patients with pPBC and only in 1 among 20 non-progressive PBC persons (5%). Anti-M4 serum concentration exceeded 400 RU/mL in 2 of the progressive patients and none in the non-progressive group. In contrast, anti-M9 serum concentration was below 100 RU/mL in all patients with pPBC, and higher than 100 RU/mL in 11 women (55%)among the non-progressive group.CONCLUSION: Females with elevated alkaline phosphatase and high anti-M2 and anti-M4 concentrations are at a high risk for developing pPBC. Quantitative AMA detection should be considered as a method for early diagnosis of pPBC. | Robert Flisiak Maria Pelszynska Danuta Prokopowicz Magdalena Rogalska Urszula Grygoruk | 2005 | World Journal of Gastroenterology2005，11，36： | 16 |
| 7 | Blood platelet and monocyte activations and relation to stages of liver cirrhosis显示文摘AIM: Blood platelets (plt) and monocytes are the cells that play a crucial role in the pathogenesis of liver damage and liver cirrhosis (LC). In this paper, the analysis of mutual relationship between platelets and monocytes activation in LC was conducted.METHODS: Immunofluorescent flow cytometry was usedto measure the percentage of activated platelet populations(CD62P, CD63), the percentage of plt-monocyte aggregates (pma) (CD41/CD45), and activated monocytes (CD11b, CD14, CD16) in the blood of 20 volunteers and 40 patientswith LC. Platelet activation markers: sP-selectin, platelet factor 4 (PF4), beta-thromboglobulin (βTG) and monocyte chemotactic peptide-1 (MCP-1) were measured and compared in different stages of LC.RESULTS: Platelet activation with the increase in bothβTG serum concentration and elevation of plt population(CD62P and CD63 as well as MIF CD62P and CD63) is elevated as LC develops and thrombocytopenia rises. There is a positive correlation between medial intensityof fluorescence (MIF) CD62P and MIF CD63 in LC. We did not show any relationship between monocyte activation and pma level. SP-selectin concentration correlates positively with plt count and pma, and negatively with stage of plt activation and MIF CD62P and MIF CD63. There was no correlation between MCP-1 concentration andpit, monocyte activation as well as pma level in LC. CD16 monocytes and MIF CD16 populations are significantlyhigher in the end stage of LC. A positive correlation occurs between the value of CD11b monocyte population andMIF CD14 and MIF CD16 on monocytes in LC.CONCLUSION: Platelet and monocyte activation plays an important role in LC. Platelet activation stage does not influence monocyte activation and production of plt aggregates with monocytes in LC. With LC development, thrombocytopenia may be the result of plt consumption in platelet-monocyte aggregates. | Anatol Panasiuk Janusz Zak Edwina Kasprzycka Katarzyna Janicka Danuta Prokopowicz | 2005 | World Journal of Gastroenterology2005，11，18： | 11 |
| 8 | Endothelial dysfunction in inflammatory bowel diseases:Pathogenesis,assessment and implications显示文摘Endothelial dysfunction is considered one of the etiological factors of inflammatory bowel disease(IBD). An inflammatory process leads to functional and structural changes in the vascular endothelium. An increase of leukocyte adhesiveness and leukocyte diapedesis, as well as an increased vascular smooth muscle tone and procoagulant activity is observed. Structural changes of the vascular endothelium comprise as well capillary and venule remodeling and proliferation of endothelial cells. Hypoxia in the inflammatory area stimulates angiogenesis by upregulation of vascular endothelial growth factor, fibroblast growth factor and tumor necrosis factor-α. Inflammatory mediators also alter the lymphatic vessel function and impair lymph flow, exacerbating tissue edema and accumulation of dead cells and bacteria. The endothelial dysfunction might be diagnosed by the use of two main methods: physical and biochemical. Physical methods are based on the assessment of large arteries vasodilatation in response to an increased flow and receptors stimulation. Flowmediated vasodilatation(FMD) is the method that is the most widely used; however, it is less sensitive in detecting early changes of the endothelium function. Most of the studies demonstrated a decrease of FMD in IBD patients but no changes in the carotic intima-media thickness. Biochemical methods of detecting the endothelial dysfunction are based on the assessment of the synthesis of compounds produced both by the normal and damaged endothelium. The endothelial dysfunction is considered an initial step in the pathogenesis of atherosclerosis in the general population. In IBD patients, the risk of cardiovascular diseases is controversial. Large, prospective studies are needed to establish the role of particular medications or dietary elements in the endothelial dysfunction as well to determine the real risk of cardiovascular diseases. | Dorota Cibor Renata Domagala-Rodacka Tomasz Rodacki Artur Jurczyszyn Tomasz Mach Danuta Owczarek | 2016 | World Journal of Gastroenterology2016，22，3： | 10 |
| 9 | Global methylation status of sperm DNA in carriers of chromosome structural aberrations显示文摘男不孕可能清楚地在人的精子与异常 DNA methylation 模式被联系。在氧化应力和精子染色体的全球 methylation 地位之间的一个协会也被建议了。现在的学习的目的是决定全球精子 DNA methylation 地位是否在染色体的搬运人的精子被影响结构的错误。在 5-methylcytosine 之间的关系(m 5 C ) 在精子和染色质正直地位的层次被评估。学习病人包括了染色体的男搬运人有繁殖失败的结构的错误(n = 24 ) ,并且控制包括了 normozoospermic 精子志愿者(n = 23 ) 。全球 m 5 C 水平用薄层的层析(TLC ) 和 immunofluorescence 被测量(如果) 技术。精子染色质正直用染色的苯胺蓝色(AB ) 和 TUNEL 试金被估计。吝啬的 m 5 C 层次在调查染色体之间是类似的结构的错误搬运人(P) 和控制(K) 。然而,精子染色质完整测试比在控制在 chromosomal 重新整理搬运人揭示了显著地更高的值(P <0.05 ) 。尽管在精子染色质正直地位和精子 DNA 破碎和 m 5在两个都分析的组( P 对 K )并列的 C 水平以一种清楚地相反的方式被代表,低染色质正直可能与在染色体的搬运人观察的精子 DNA 的高 hypomethylation 地位被联系结构的错误。 | Marta Olszewska Miroslawa Z Barciszewska Monika Fraczek Nataliya Huleyuk Vyacheslav B chernykh Danuta Zastavna Jan Barciszewski Maciej Kurpisz | 2017 | Asian Journal of Andrology2017，19，1： | 8 |
| 10 | Effect of lamivudine treatment on plasma levels of transforming growth factor β_1,tissue inhibitor of metalloproteinases-1 and metalloproteinase-1 in patients with chronic hepatitis B显示文摘AIM: Transforming growth factor (TGF)-β1, metalloproteinase (MMP)-1 and its tissue inhibitor (TIMP)-I are considered predictive biomarkers of chronic hepatitis activity and fibrosis.The aim of this study was to evaluate the effect of lamivudine treatment on the plasma levels of these peptides in patients with chronic hepatitis B.METHODS: TGF-β1, MMP-1 and TIMP-1 plasma concentrations were measured with an enzyme immunoassay in 40 patients treated with lamivudine for 48 wk. Elimination of HBV-DNA and HBV antigens was evaluated 24 wk after treatment completion.RESULTS: Baseline TGF-β1(29.6±2.2 ng/mL) and TIMP-1(1 578±93 ng/mL) significantly exceeded normal values(18.3±1.6 ng/mL and 1 102±67 ng/mL respectively). Lamivudine treatment resulted in a significant decrease of TGF-β1 and TIMP-1 during treatment with an increase after 24 wk of treatment. Pretreatment MMP-1 levels (6.7±0.7 ng/mL) were significantly lower than normal values (11.9±0.9 ng/mL) and increased during treatment and follow-up. A significant correlation was noted between TGF-β1 or TIMP-1 and aminotransferases as well as fibrosis scored in liver biopsy specimens. There were no statistically significant differences of TGF-β1, TIMP-1 and MMP-1 between four groups at baseline, 24 and 48 wk of treatment. TGF-β1 and TIMP-1 levels increased significantly in non-responders and normalized in responders at wk 72. MMP-1 also normalized in responders and decreased to values significantly lower than normal in non-responders.CONCLUSION: These findings support the role of TGF-β1,TIMP-1 and MMP-1 in the pathogenesis of chronic hepatitis B.Because of their association with hepatic injury and antiviral treatment efficacy, determination of these peptides may be useful in disease management. | Robert Flisiak Haza Al-Kadasi Jerzy Jaroszewicz Danuta Prokopowicz Iwona Flisiak | 2004 | World Journal of Gastroenterology2004，10，18： | 7 |
| 11 | Serum concentration of sFas and sFasL in healthy HBsAg carriers,chronic viral hepatitis B and C patients显示文摘AIM: To estimate the amount of apoptosis among healthy HBsAg carriers, patients with chronic HBV infection treated with lamivudine and patients with chronic HCV infedJon treated with interferon alpha and ribavirin. Activity of apoptosis was evaluated by serum sFas/sFasL concentration measurement.Moreover dependence between apoptosis and HBV-DNA or HCV-RNA levels was studied.METHODS: Eighty-six persons were included into study: 34 healthy HBsAg carriers, 33 patients with chronic HBV infection and 19 patients with chronic HCV infection. Serum levels of sFas/sFasL were measured by ELISA assay. HBV-DNA and HCV-RNA were measured by RT-PCR assay. Levels of sFas/sFasL were determined before and 2 and 12 wk after therapy in patients with chronic hepatitis B and C infection.HBV-DNA or HCV-RNA was detected before treatment and 6 mo after treatment.RFSULTS: Twenty-four (71%) healthy HBsAg carriers showed HBV-DNA over 10^5/mL, which was comparable to the patients with chronic hepatitis B. Independently from HBV-DNA levels,the concentration of sFas among healthy HBsAg carriers was comparable to healthy persons. Among patients with chronic hepatitis B and C, the concentration of sFas was significantly higher in comparison to healthy HBsAg carriers and healthy persons. In chronic hepatitis B patients the concentration of sFas was decreased during lamivudine treatment. Among chronic hepatitis C patients the concentration of sFas was increased during IFN alpha and ribavirin treatment, sFasL was not detected in control group. Furthermore sFasL oo:urred more frequently in chronic hepatitis C patients in comparison to chronic hepatitis B patients.CONCLUSION: There are no correlations between apoptosis and HBV-DNA levels. However ther is an association between apoptosis and activity of inflammation in patients with chronic HBV infection. Apoptosis can be increased in patients with chronic hepatitis C by effective treatment which may be a result of apoptosis stimulation by IFN-α. | Tadeusz Wojciech Lapinski Oksana Kowalczuk Danuta Prokopowicz Lech Chyczewski | 2004 | World Journal of Gastroenterology2004，10，24： | 7 |
| 12 | Effect of pegylated interferon alpha 2b plus ribavirin treatment on plasma transforming growth factor-β1,metalloproteinase-1,and tissue metalloproteinase inhibitor-1 in patients with chronic hepatitis C显示文摘AIM: To evaluate the effect of antiviral treatment on plasma levels of transforming growth factor-β1 (TGF-β1),metalloproteinase 1 (MMP-1), and tissue inhibitor of metalloproteinase-1 (TIMP-1) in patients with chronic hepatitis C.METHODS: TGF-β1, MMP-1, and TIMP-1 plasma concentrations were measured by an enzyme immunoassay in 28 patients, during 48 wk of treatment with pegylated interferon-alpha 2b (PEG-IFN-α2b) plus ribavirin (RBV)and after 24 wk of follow-up. Patients were divided into two groups: responders (R) and non-responders (NR)related to achieved sustained virologic response. Normal values were evaluated in plasma samples of 13 healthy volunteers.RESULTS: Baseline plasma concentrations of TGF-β1and TIMP-1 (30.9±3.7 and 1506±61 ng/mL respectively)measured in all subjects significantly exceeded the normal values (TGF-β1:18.3±1.6 ng/mL and TIMP-1:1102±67 ng/mL). In contrast, pretreatment MMP-1mean level (6.5±0.9 ng/mL) was significantly lower than normal values (11.9±0.9 ng/mL). Response to the treatment was observed in 12 patients (43%). TGF-β1mean concentration measured during the treatment phase decreased to the control level in both groups.However at wk 72, values of NR patients increased and became significantly higher than in R group.TIMP-1 concentrations in R group decreased during the treatment to the level similar to normal. In NR group,TIMP-1 remained significantly elevated during treatment and follow-up phase and significant difference between both groups was demonstrated at wk 48 and 72. MMP-1levels were significantly decreased in both groups at baseline. Treatment caused rise of its concentration only in the R group, whereas values in NR group remained on the level similar to baseline. Statistically significant difference between groups was noted at wk 48 and 72.CONCLUSION: These findings support the usefulness of TGF-β1, TIMP-1, and MMP-1 in the management of chronic hepatitis C. Elevated TIMP-1 and low MMP-1plasma concentrations during antiviral therapy may indicate medication failure. | Robert Flisiak Jerzy Jaroszewicz Tadeusz W Lapiski Iwona Flisiak Danuta Prokopowiczi | 2005 | World Journal of Gastroenterology2005，11，43： | 7 |
| 13 | Phagocytic and oxidative burst activity of neutrophils in the end stage of liver cirrhosis显示文摘AIM: To evaluate the phagocytic activity and neutrophil oxidative burst in liver cirrhosis.METHODS: In 45 patients with advanced postalcoholic liver cirrhosis (aged 45±14 years) and in 25 healthy volunteers (aged 38±5 years), the percentage of phagocytizing cells after in vitro incubation with E. coli (Phagotest Kit), phagocytic activity (mean intensity of fluorescence, MIF) and the percentage of neutrophil oxidative burst (Bursttest Kit), and the level of free oxygen radical production (MIF of Rodamine 123)were analyzed by flow cytometry. The levels of soluble sICAM-1, sVCAM-1, sP-selectin, sE-selectin, sL-selectin,and TNF-α were determined in blood serum.RESULTS: The percentage of E. coli phagocytizing neutrophils in liver cirrhosis patients was comparable to that in healthy subjects. MIF of neutrophil - ingested E. coli was higher in patients with liver cirrhosis. The oxidative burst in E. coli phagocytizing neutrophils generated less amount of active oxygen compounds in liver cirrhosis patients (MIF of R123:24.7±7.1 and 29.7±6.6 in healthy,P<0.01). Phorbol myristate acetate (PMA) - stimulated neutrophilsproduced less reactive oxidants in liver cirrhosis patients than in healthy subjects (MIF of R123: 42.7±14.6 vs 50.2±13.3, P<0.01). A negative correlation was observed between oxidative burst MIF of PMA-stimulated neutrophils and ALT and AST levels (r -0.35, P<0.05;r-0.4, P<0.03). sVCAM-1, sICAM-1, sE-selectin concentrations correlated negatively with the oxygen free radical production (MIF of R123) in neutrophils after PMA stimulation in liver cirrhosis patients (r-0.45, P<0.05;r-0.41, P<0.05; r-0.39, P<0.05, respectively).CONCLUSION: Neutrophil metabolic activity diminishes together with the intensification of liver failure. The metabolic potential of phagocytizing neutrophils is significantly lower in liver cirrhosis patients, which can be one of the causes of immune mechanism damage. The evaluation of oxygen metabolism of E. coli-stimulated neutrophils reveals that the amount of released oxygen metabolites is smaller in liver cirrhosis patients than in healthy subjects. | Anatol Panasiuk Jolanta Wysocka Elzbieta Maciorkowska Bozena Panasiuk Danuta Prokopowicz Janusz Zak Karol Radomski | 2005 | World Journal of Gastroenterology2005，11，48： | 4 |
| 14 | IL28B polymorphism as a predictor of antiviral response in chronic hepatitis C显示文摘AIM:To evaluate the effect of single nucleotide polymorphisms of interleukin(IL)-28B,rs12979860 on progression and treatment response in chronic hepatitis C.METHODS:Patients(n = 64;37 men,27 women;mean age,44 ± 12 years) with chronic hepatitis C,genotype 1,received treatment with peg-interferon plus ribavirin.Genotyping of rs12979860 was performed on peripheral blood DNA.Histopathological assessment of necroinflammatory grade and fibrosis stage were scored using the METAVIR system on a liver biopsy sample before treatment.Serum viral load,aminotransferase activity,and insulin level were measured.Insulin resistance index,body mass index,waist/hip ratio,percentage of body fat and fibrosis progression rate were calculated.Applied dose of interferon and ribavirin,platelet and neutrophil count and hemoglobin level were measured.RESULTS:A sustained virological response(SVR) was significantly associated with IL28B polymorphism(CC vs TT allele:odds ratio(OR),25;CC vs CT allele:OR,5.4),inflammation activity(G < 1 vs G > 1:OR,3.9),fibrosis(F < 1 vs F > 1:OR,5.9),platelet count(> 200 × 10 9 /L vs < 200 × 10 9 /L:OR,4.7;OR in patients with genotype CT:12.8),fatty liver(absence vs presence of steatosis:OR,4.8),insulin resistance index(< 2.5 vs > 2.5:OR,3.9),and baseline HCV viral load(< 10 6 IU/mL vs > 10 6 IU/mL:OR,3.0).There was no association with age,sex,aminotransferases activity,body mass index,waist/hip ratio,or percentage body fat.There was borderline significance(P = 0.064) of increased fibrosis in patients with the TT allele,and no differences in the insulin resistance index between groups of patients with CC,CT and TT alleles(P = 0.12).Spearman's rank correlation coefficient between insulin resistance and stage of fibrosis and body mass index was r = 0.618 and r = 0.605,respectively(P < 0.001).Significant differences were found in the insulin resistance index(P = 0.01) between patients with and without steatosis.Patients with the CT allele and absence of a SVR had a higher incidence of requiring threshold dose reduction of interferon(P = 0.07).CONCLUSION:IL28B variation is the strongest host factor not related to insulin resistance that determines outcome of antiviral therapy.Baseline platelet count predicts the outcome of antiviral therapy in CT allele patients. | Andrzej Ciela Monika Bociaga-Jasik Iwona Sobczyk-Krupiarz Mikolaj K Glowacki Danuta Owczarek Dorota Cibor Marek Sanak Tomasz Mach | 2012 | World Journal of Gastroenterology2012，18，35： | 4 |
| 15 | Epidemiological characteristics of inflammatory bowel disease in North-Eastern Poland显示文摘AIM: To provide the clinical and epidemiological data of inflammatory bowel disease (IBD) patients of NorthEastern Poland.METHODS: A total of 248 IBD patients diagnosed and hospitalized in the Department of Infectious Diseases in Bialystok between 1990 and 2003 were included in the study. We analyzed age, sex, education, characteristics of job, type of the environment, discontinuation of employment due to IBD, colitis extent, need of surgical treatment, and coexistence of other diseases.RESULTS: Two hundred and thirty-three IBD patients (94%) were diagnosed as ulcerative colitis (UC), and only 15 (6%) were diagnosed as Crohn's disease (CD). Patients with CD were significantly younger at the time of diagnosis and male predominance was observed. The mean age of the patients at the time UC diagnosis was 44.9±1.1 years. Histogram of the age of patients showed the characteristic biphasic distribution with two peaks between 20 and 40 years and between 60 and 70 years. The predominant form of UC was left sided colitis, which affected almost 80% of the studied population. The most extensive form - pancolitis was present in 34 patients (15%). Only 6% of UC patients required surgery, whereas 36% of CD patients underwent surgery (P<0.005). Among coexisting disorders, cholelithiasis was the most prevalent and demonstrated in 35 patients (14%), pulmonary disorders were diagnosed in 2%, and psoriasis in 1.4%.Since 1998, the number of admitted IBD patients has slightly increased.CONCLUSION: Occurrence of UC in Poland is much higher than that of CD. The majority of UC cases are diagnosed in young people (20-40 years) with the predominance of male patients. The most common clinical form of UC is left sided colitis. | Alicja Wiercinska-Drapalo Jerzy Jaroszewicz Robert Flisiak Danuta Prokopowicz | 2005 | World Journal of Gastroenterology2005，11，17： | 3 |
| 16 | Levels and activities of von Willebrand factor and metalloproteinase with thrombospondin type-1 motif, number 13 in inflammatory bowel diseases显示文摘AIM To evaluate the levels of von Willebrand factor(VWF) and metalloproteinase with thrombospondin type-1 motif, number 13(ADAMTS13) in inflammatory bowel disease(IBD) and correlate them with the disease activity.METHODS Consecutive patients with IBD aged 18 years or older were enrolled in the study. Forty-seven patients with ulcerative colitis(UC), 38 with Crohn's disease(CD), and 50 healthy controls were included. The white blood cell count, haematocrit, platelet count, fibrinogen, partial activated thromboplastin time, C-reactive protein, albumin, VWF antigen level(VWF:Ag), VWF ristocetin cofactor activity(VWF:RCo), VWF collagen-binding activity(VWF:CB), and ADAMTS13 antigen level(ADAMTS13:Ag) and activity(ADAMTS13act) were measured. The following ratios were assessed: V W F : R C o/V W F : A g, V W F : C B/V W F : A g, V W F : A g/ADAMTS13 act, and ADAMTS13act/ADAMTS13:Ag. RESULTS Compared to controls, the odds ratio(OR) of an elevated VWF: Ag > 150% was 8.7(95%CI: 2.7-28.1) in the UC group and 16.2(95%CI: 4.8-54.0) in the CD group. VWF:CB was lower in UC patients, and active CD was associated with a higher VWF: RCo(+38%). The ORs of VWF:CB/VWF:Ag < 0.7(a marker of acquired von Willebrand syndrome) in the UC and CD groups were 11.9(95%CI: 4.4-32.4) and 13.3(95%CI: 4.6-38.1), respectively. Active UC was associated with lower ADAMTS13:Ag(-23%) and ADAMTS13act(-20%) compared to UC in remission. Patients with active CD had a 15% lower ADAMTS13 act than controls. The activity of UC, but not that of CD, was inversely correlated with ADAMTS13:Ag(r =-0.76) and ADAMTS13act(r =-0.81). CONCLUSION Complex VWF-ADAMTS13-mediated mechanisms disturb haemostasis in IBD. A reduced WVF:CB is a risk factor for bleeding, while a lower ADAMTS13 level combined with an elevated VWF:Ag could predispose one to thrombosis. | Dorota Cibor Danuta Owczarek Saulius Butenas Kinga Salapa Tomasz Mach Anetta Undas | 2017 | World Journal of Gastroenterology2017，23，26： | 3 |
| 17 | Towards standardizing retinal optical coherence tomography angiography:a review显示文摘The visualization and assessment of retinal microvasculature are important in the study,diagnosis,monitoring,and guidance of treatment of ocular and systemic diseases.With the introduction of optical coherence tomography angiography(OCTA),it has become possible to visualize the retinal microvasculature volumetrically and without a contrast agent.Many lab-based and commercial clinical instruments,imaging protocols and data analysis methods and metrics,have been applied,often inconsistently,resulting in a confusing picture that represents a major barrier to progress in applying OCTA to reduce the burden of disease.Open data and software sharing,and cross-comparison and pooling of data from different studies are rare.These inabilities have impeded building the large databases of annotated OCTA images of healthy and diseased retinas that are necessary to study and define characteristics of specific conditions.This paper addresses the steps needed to standardize OCTA imaging of the human retina to address these limitations.Through review of the OCTA literature,we identify issues and inconsistencies and propose minimum standards for imaging protocols,data analysis methods,metrics,reporting of findings,and clinical practice and,where this is not possible,we identify areas that require further investigation.We hope that this paper will encourage the unification of imaging protocols in OCTA,promote transparency in the process of data collection,analysis,and reporting,and facilitate increasing the impact of OCTA on retinal healthcare delivery and life science investigations. | Danuta M.Sampson Adam M.Dubis Fred K.Chen Robert J.Zawadzki David D.Sampson | 2022 | Light(Science & Applications)2022，11，4： | 3 |
| 18 | 严重肢体缺血患者血浆中促血管生成潜力与血浆MMP-9、TIMP-1和TIMP-2水平升高的相关性研究(英文)显示文摘本研究通过比较间歇性跛行(IC)和严重肢体缺血(CLI)患者的血液参数,选择合适的临床和生化特指标,以评估促血管生成的潜力和抑制血管生成的作用.结果表明,通过刺激下肢动脉疾病(LEAD)病人血浆中的血管生成,内皮生长因子A(VEGF-A)浓度会显著增加,同时依赖于循环受体sVEGFR-1和sVEGFR-2的抑制也会显著减少.与IC病人相比,CLI病人具有较高的VEGF-A、金属蛋白酶9(MMP-9)、金属蛋白酶组织抑制因子1(TIMP-1)和TIMP-2浓度. | Radoslaw Wieczór Anna Maria Wieczór Arleta Kulwas Grzegorz Pulkowski Jacek Budzyński Danuta Rosc | 2019 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2019，20，8： | 3 |
| 19 | 血糖控制良好的2型糖尿病患者血清VEGF-A及其受体VEGFR1和VEGFR2含量检测(英文)显示文摘研究目的:探讨血糖控制良好的2型糖尿病患者血管内皮生长因子-A(VEGF-A)及其受体VEGFR1和VEGFR2含量及其临床意义。研究方法:本文以31例血糖得到良好控制且没有明显大血管或微血管病变的2型糖尿病患者作为研究对象(实验组),30位健康志愿者为对照组,同期检测患者及健康志愿者的空腹血糖、血脂、糖化血红蛋白水平,及血清VEGF-A、VEGFR1和VEGFR2含量,并加以比较和统计学分析。重要结论:研究结果表明,血糖控制良好的2型糖尿病患者血清中的VEGF-A及其受体VEGFR1和VEGFR2的含量和健康志愿者基本一致,两者无统计学意义,这可能显示血糖水平的合理控制能延缓血管并发症的产生。同时,2型糖尿病患者的血清VEGFR2含量和高密度脂蛋白胆固醇水平之间存在负相关,而血清VEGF-A、VEGFR2含量和甘油三脂水平之间存在正相关,这也表明糖尿病患者的血脂紊乱可能参与了血管生成的调节。 | Barbara RUSZKOWSKA-CIASTEK Alina SOKUP Maciej W. SOCHA Zofia RUPRECHT Lidia HALAS Barbara GóRALCZYK Krzysztof GóRALCZYK Grazyna GADOMSKA Danuta ROSC | 2014 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2014，15，6： | 2 |
| 20 | 天然恒牙列的动态咬合接触形式显示文摘目的:在既往研究中.从未停止过关于修复和正畸治疗中进行咬合重建时.应该建立何种形式的咬合接触的讨论。对天然恒牙列的动态咬合的流行病学调查将有助于走出这种持续讨论的困境。本研究的目的就是要通过调查研究.总结年青群体和老年群体天然牙列的特征性动态咬合接触形式,并且分析动态咬合接触形式与性别和年龄之间的相关性。材料和方法:本研究共调查了834人。所有的调查对象都具有天然牙列.并且没有修复体存在。调查对象的年龄范围是20--63岁。在调查对象进行牙诱导的侧方和前伸运动过程中,检查天然牙的动态咬合接触形式。结果:结果发现存在以下的动态咬合接触形式:双侧和单侧的尖牙保护[牙合],组牙功能[牙合].平衡[牙合]以及“其他[牙合]型”。男性和女性之间不同类型的动态咬合接触形式的出现概率相同。但是.一些咬合接触形式的出现率与年龄有相关性。随着年龄的增加.双侧和单侧尖牙保护耠的出现率出现下降趋势,而组牙功能[牙合]的出现率上升。平衡[牙合]以及“其他[牙合]型”的出现率很低.并且没有呈现与年龄相关的变化趋势。结论:双侧尖牙保护[牙合]和组牙功能[牙合]是年青患者的特征性咬合接触形式.而老年患者中组牙功能[牙合]的接触形式更加普遍。当年青患者接受修复和正畸手段的咬合重建时.尖牙保护[牙合]是一种最适合的咬合接触形式,而对于老年患者来说.组牙功能[牙合]则更加适合。需要进一步的研究来探讨[牙合]型和牙周炎、磨牙症或者颞下颌关节紊乱病之间的关系。 | Halina Panek Teresa Matthews-Brzozowska Danuta Nowakowska Beata Panek Grzegorz Bielicki Slawomir Makacewicz Malgorzata Mank iewicz 李健(译) 姜婷(审) | 2009 | 中国口腔医学继续教育杂志2009，12，6： | 2 |
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