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| 1 | Cryopreservation for delayed circulating tumor cell isolation is a valid strategy for prognostic association of circulating tumor cells in gastroesophageal cancer显示文摘AIM To demonstrate the feasibility of cryopreservation of peripheral blood mononuclear cells(PBMCs) for prognostic circulating tumor cell(CTC) detection in gastroesophageal cancer.METHODS Using 7.5 m L blood samples collected in EDTA tubes from patients with gastroesopheagal adenocarcinoma, CTCs were isolated by epithelial cell adhesion molecule based immunomagnetic capture using the Iso Flux platform. Paired specimens taken during the same blood draw(n = 15) were used to compare number of CTCs isolated from fresh and cryopreserved PBMCs. Blood samples were processed within 24 h to recover the PBMC fraction, with PBMCs used for fresh analysis immediately processed for CTC isolation. Cryopreservation of PBMCs lasted from 2 wk to 25.2 mo(median 14.6 mo). CTCs isolated from pre-treatment cryopreserved PBMCs(n = 43) were examined for associations with clinicopathological variables and survival outcomes.RESULTS While there was a significant trend to a decrease in CTC numbers associated with cryopreserved specimens(mean number of CTCs 34.4 vs 51.5, P = 0.04), this was predominately in samples with a total CTC count of > 50, with low CTC count samples less affected(P = 0.06). There was no significant association between the duration of cryopreservation and number of CTCs. In cryopreserved PBMCs from patient samples prior to treatment, a high CTC count(> 17) was associated with poorer overall survival(OS)(n = 43, HR = 4.4, 95%CI: 1.7-11.7, P = 0.0013). In multivariate analysis, after controlling for sex, age, stage, ECOG performance status, and primary tumor location, a high CTC count remained significantly associated with a poorer OS(HR = 3.7, 95%CI: 1.2-12.4, P = 0.03). CONCLUSION PBMC cryopreservation for delayed CTC isolation is a valid strategy to assist with sample collection, transporting and processing. | Daniel Brungs David Lynch Alison WS Luk Elahe Minaei Marie Ranson Morteza Aghmesheh Kara L Vine Martin Carolan Mouhannad Jaber Paul de Souza Therese M Becker | 2018 | World Journal of Gastroenterology2018，24，7： | 5 |
| 2 | Resting heart rate pattern during follow-up and mortality in hypertensive patients显示文摘 | Paul L Hastie CE Li WS | 2010 | Hypertension2010，55，2： | 1 |
| 3 | Agenesis of the corpus callosum: genetic, developmental and functional aspects of connectivity 显示文摘 | Paul LK Brown WS Adolphs R | 2007 | Nat Rev Neurosci2007，8，4： | 1 |
| 4 | Regulation of the CgPdrl transcription factor from the pathogen Candida glabrata 显示文摘 | Paul S Schmidt JA Moye - Rowley WS | 2011 | Eukaryot Cell2011，10，2： | 1 |
| 5 | Outbreak of Japanese encephalitis on the island of Saipan, 1990显示文摘 | Paul WS Moore PS Karabatsos N | 1993 | J Infect Dis1993，167，5： | 1 |
| 6 | Resting heart rate pattern during follow-up and mortality in hypertensive patients 显示文摘 | Paul L Hastie CE Li WS | 2010 | Hypertension2010，55，2： | 1 |
| 7 | Novel polymeric microspheres containing norcantharidin for chemoembolization显示文摘 | Liu X Heng WS Paul | 2006 | J Control Release2006，116，1： | 1 |
| 8 | Agenesis of the corpus callosum: genetic, developmental and functional aspects of connectivity显示文摘 | Paul LK Brown WS Adolphs R | 2007 | Nat Rev Neurosci2007，8，4： | 1 |
| 9 | Agenesis of the corpus callosum:genetic,developmental and functional aspects of connectivity显示文摘 | Paul LK Brown WS Adolphs R | 2007 | Nat Rev Neurosci2007，8，4： | 1 |
| 10 | Resting heart rate pattern dur- ing follow up and mortality in HT显示文摘 | Paul L Hastie CE Li WS | 2010 | Hypertension2010，55，3： | 1 |
| 11 | Cervical spinal motion during intubation: efficacy ofstabilization maneuvers in the setting of complete segmental instability显示文摘 | Peter JL Darin WS Paul DS | 2001 | Journal ofNeurosurgery Spine2001，94，2： | 1 |
| 12 | Risk factors for residual and recurrent uveal melanoma after trans-scleral local resection显示文摘 | DamatoBE Paul J Foulds WS | 1996 | Br J Ophthalomol1996，80，： | 1 |
| 13 | Risk factors for metastatic uvealmelanoma after trans-scleral local resection显示文摘 | Damato BE Paul J Foulds WS | 1996 | Br J Iphthalmal1996，80，： | 1 |
| 14 | Coating of Multiparticulates for Sustained Release显示文摘 | Elaine SK Chan L W Paul WS | 2005 | American Journal of Drug Delivery2005，3，1： | 1 |
| 15 | Activity of the investigational fluoroquinolone finafloxacin against ciprofloxacin-sensitive and resistant acinetobacter baumannii isolates显示文摘 | Paul GH Hilmar WS Wisplinghoff | 2010 | Antimicrob Agents Chemother2010，54，4： | 1 |
| 16 | Agenesis of the corpus callosum: genetic developmental and functional aspects of connectivity显示文摘 | Paul LK Brown WS Adolphs R | 2007 | Nat Rev Neurosci2007，8，： | 1 |
| 17 | Identification of genomic binding sites for Candida glabrata Pdrl transcription factor in wild-type and rho0 cells显示文摘 | Paul S Bair TB Moye-Rowley WS | 2014 | Antimicrob Agents Chemother2014，58，11： | 1 |
| 18 | Predictive factors of visual outcome after local resection of choroidal melamoma 显示文摘 | Damato BE Paul J Foulds WS | 1993 | Br J Ophthalmol1993，77，10： | 1 |
| 19 | Surfactant protein A prevents silica-mediated toxicity to rat alveolar macrophages 显示文摘 | Robert WS Paul Wisniowski Diane LK | 2000 | Am J Physiol Lung Cell Mol Physiol2000，278，： | 1 |
| 20 | Regulation of the Cg- Pdrl transcription factor from the pathogen Candida glabrata显示文摘 | Paul S Schmidt JA Moye-Rowley WS | 2011 | Eukaryot Cell2011，10，2： | 1 |
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