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| 1 | Investigation of cholecystokinin receptors in the human lower esophageal sphincter显示文摘AIM:To compare the binding of cholecystokinin(CCK)-8to CCK receptors in sling and clasp fibers of the human lower esophageal sphincter.METHODS:Esophageal sling and clasp fibers were isolated from eight esophagectomy specimens,resected for squamous cell carcinoma in the upper two thirds of the esophagus,which had been maintained in oxygenated Kreb’s solution.Western blot was used to measure CCK-A and CCK-B receptor subtypes in the two muscles.A radioligand binding assay was used to determine the binding parameters of 3H-CCK-8S to the CCK receptor subtypes.The specificity of binding was determined by the addition of proglumide,which blocks the binding of CCK to both receptor subtypes.RESULTS:There was no significant difference between the sling and clasp fibers of the human lower esophageal sphincter in the amount of CCK-A[integratedoptical density(IOD)value:22.65±0.642 vs 22.328±1.042,P=0.806]or CCK-B receptor protein(IOD value:13.20±0.423 vs 12.45±0.294,P=0.224)as measured by Western blot.The maximum binding of radio-labeled CCK-8S was higher in the sling fibers than in the clasp fibers(595.75±3.231 cpm vs 500.000±10.087 cpm,P<0.001)and dissociation constant was lower(Kd:1.437±0.024 nmol/L vs 1.671±0.024nmol/L,P<0.001).The IC50 of the receptor specific antagonists were lower for the CCK-A receptors than for the CCK-B(P<0.01).CONCLUSION:CCK binding modulates the contractile function of the lower esophageal sphincter through differential binding to the CCK-A receptor on the sling and clasp fibers. | Jun-Feng Liu Jian Zhang Xin-Bo Liu Paul A Drew | 2014 | World Journal of Gastroenterology2014，20，21： | 3 |
| 2 | Titanium-based composites produced by powder metallurgy 显示文摘 | Wanjara P Yue S Drew R A L | 1997 | Key Eng Mater1997，，： | 2 |
| 3 | Methylation of TIMP3 in esophageal squamous cell carcinoma显示文摘AIM: To measure the frequency of DNA methylation of the tissue inhibitor of metalloproteinase 3 (TIMP3) promoter and relate this to any change of gene expression in esophageal squamous cell carcinoma in patients from a region of high incidence in China.METHODS: Cancer cell lines were treated with or without the demethylating reagent 5-aza-2’-deoxycytidine. Methylation of the TIMP3 promoter was assessed in three regions by melt curve analysis and its expression was assessed by real-time RT-PCR. Tumors and proximal resection margins were obtained from 64 patients with esophageal squamous cell carcinoma from a region of high incidence in China. Methylation was assessed by melt curve analysis and expression by immunohistochemistry.RESULTS: Methylation in one of the three promoter regions assessed correlated with gene silencing in esophageal cell lines. A degree of methylation of TIMP3 was found in only four esophageal squamous cell carcinomas, and partial loss of TIMP3 protein expression in just one.CONCLUSION: Methylation and loss of expression of TIMP3 occurs infrequently in esophageal squamous cell carcinoma in a region of high incidence in China. | Eric Smith Neville J De Young Zi-Qiang Tian Maria Caruso Andrew R Ruszkiewicz Jun-Feng Liu Glyn G Jamieson Paul A Drew | 2008 | World Journal of Gastroenterology2008，14，2： | 2 |
| 4 | The interaction of reaction-bonded silicon carbide and Inconel 600 with a nickel-based brazing alloy显示文摘 | Mcdermid J R Pugh M D Drew R A L | 1989 | Metallurgical Transactions A1989，20，： | 1 |
| 5 | Electrical impedance scanning:a new imaging technique for evaluating microcalcification in the breast显示文摘 | Kneeshaw PJ Drew PJ Hubbard A | 2002 | Breast Cancer Res2002，4，1： | 1 |
| 6 | Monitoring and remote failure detection of grid-connected PV systems based on satellite ob- servations显示文摘 | DREWS A KEIZER A C BEYER l-I G | 2007 | Solar Energy2007，81，： | 1 |
| 7 | Main concepts of the ' European approach' to karst-groundwater-vulnerability assess- ment and mapping 显示文摘 | Daly D Dassargues A Drew D | 2002 | Hydrogeology Journal2002，10，： | 1 |
| 8 | Membrane fouling in membrane bioreactors-- Characterisation,contradictions, cause and cures 显示文摘 | Drews A | 2010 | J Membr Sci2010，363，12： | 1 |
| 9 | Longitudinal analysis of the impact of service changes on customer attitudes显示文摘 | Bolton R N Drew J H A | | 0，，： | 1 |
| 10 | Membrane fouling in membrane bioreactors-Characterisation,contradictions,cause and cures显示文摘 | Drews A | | 0，，1： | 1 |
| 11 | An in vitro method, based on chewing, to Predict resistant starch content in foods allows parallel determination of potentially available starch and dietary fiber 显示文摘 | Akerberg A K E Liljeberg H G M Granfeldt Y E Drews A W Bjorck I M E | 1998 | Journal of Nutrition1998，128，3： | 1 |
| 12 | Multiple paternity in a natural population of a wild tobacco fly, Bactrocera cacuminata (Diptera: Tephritidae), assessed by microsatellite DNA markers 显示文摘 | Song S D Drew R A Hughes J M | 2007 | Molecular Ecology2007，16，11： | 1 |
| 13 | Facilitation of oviposition behaviour of Bactrocera tryoini flies显示文摘 | PROKOPY R J REMING M C DREW R A I | 1999 | Journal of Insect Behavior1999，12，： | 1 |
| 14 | Tatanium based composites produced by powder metallurgy显示文摘 | Wanjara P Yue S Drew R A L | 1997 | Key Eng Mater1997，，： | 1 |
| 15 | Savoy: Absorption of methionine and 2-hydroxy-4-methylthiobutoanic acid in conventional and germ-free chickens显示文摘 | Drew M D Van Kessel A G Maer z D D | 2003 | Poultry Science2003，82，7： | 1 |
| 16 | Metallothionein 3 expression is frequently down-regulated in oesophageal squamous cell carcinoma by DNA methylation 显示文摘 | Smith E Drew P A Tian Z Q | 2005 | Mol Cancer2005，4，： | 1 |
| 17 | Carbon diox- ide capture using ionic liquid 1-butyl-3-methylimidazolium acetate 显示文摘 | Shiflett M B Drew D W Cantini R A | 2010 | Energy & Fuels2010，24，10： | 1 |
| 18 | Dentate gyms neurogenesis and depression显示文摘 | Sahay A Drew M R Hen R | 2007 | Prog Brain Res2007，163，： | 1 |
| 19 | Improvement of the mechanical properties in a nanocrystalline NiAl intermetallic alloy with Fe,Ga and Mo additions显示文摘 | ALBITER A SALAZAR M BEDOLLA E DREW R A L PEREZ R | | 0，，1： | 1 |
| 20 | Characterization of alumina-supported Pt and Pt-Pd NO oxidation catalysts with advanced electron microscopy显示文摘 | Ezekoye O K Drews A R Jen H-W | 2011 | J Catal2011，280，： | 1 |
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