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| 1 | Stress changes on major faults caused by M_w7.9 Wenchuan earthquake,May 12,2008显示文摘On May 12,2008,a magnitude 7.9 earthquake ruptured the Longmenshan fault system in Sichuan Province,China,collapsing buildings and killing tens of thousands people.As predicted,aftershocks may last for at least one year,and moreover,large aftershocks are likely to occur.Therefore,it is critical to outline the areas with potential aftershocks before reconstruction and resettling people as to avoid future disasters.It is demonstrated that the redistribution of stress induced by an earthquake should trigger successive seismic activity.Based on static stress triggering theory,we calculated the coseismic stress changes on major faults induced by the Wenchuan earthquake,with elastic dislocation the-ory and the multilayered crustal model.We also discuss the stress distribution and its significance for future seismic activity under the impact of the Wenchuan earthquake.It is shown that coulomb failure stress(CFS) increases obviously on the Daofu-Kangding segment of the Xianshuihe Fault,the Maqu and Nanping segment of the Eastern Kunlun Fault,the Qingchuan Fault,southern segment of the Min-jiang Fault,Pengxian-Guanxian Fault,Jiangyou-Guangyuan Fault,and Jiangyou-Guanxian Fault.The increased stress raises the probability of earthquake occurrence on these faults.Since these areas are highly populated,earthquake monitoring and early disaster alarm system are needed.CFS increases with a magnitude of 0.03―0.06 MPa on the Qingchuan Fault,which is close to the northern end of the rapture of Wenchuan earthquake.The occurrence of some strong aftershocks,including three events with magnitude higher than 5.0,indicates that the seismic activities have been triggered by the main shock.Aftershocks seem to migrate northwards.Since the CFS change on the Lueyang-Mianxian Fault located on the NEE of the Qingchuan Fault is rather small(±0.01 MPa),the migration of aftershocks might be terminated in the area near Hanzhong City.The CFS change on the western Qinling Fault is around 10 Pa,and the impact of static triggering can be neglected.The increment of CFS on the Pengxian-Guanxian Fault and Beichuan-Yingxiu Fault southwest to the main rupture is 0.005―0.015 MPa,which would facilitate earthquake triggering in these areas.Very few aftershocks in these areas indicate that the accumulated stress has not been released sufficiently.High seismic risk is predicated in these areas due to coseismic CFS loading.The Wenchuan earthquake released the accumulated CFS on the Fubianhe Fault,the Huya Fault,the Ha'nan-Qingshanwan Fault,and the Diebu-Bailongjiang Fault.The decrement of CFS changes on the Longquanshan Fault east to Chengdu City is about 0.002 MPa.The seismic activity will be depressed by decrement of CFS on these faults. | SHAN Bin XIONG Xiong ZHENG Yong DIAO FaQi | 2009 | Science China Earth Sciences2009，52，5： | 47 |
| 2 | Effects of penehyclidine hydrochloride on rat intestinal barrier function during cardiopulmonary bypass显示文摘AIM:To test the ability of penehyclidine hydrochloride (PHC) to attenuate intestinal injury in a rat cardiopulmonary bypass (CPB) model.METHODS:Male Sprague-Dawley rats were randomly divided into six groups (eight each):sham-operated control;sham-operated low-dose PHC control (0.6 mg/kg);sham-operated high-dose PHC control (2.0 mg/kg);CPB vehicle control;CPB low-dose PHC (0.6 mg/kg);and CPB high-dose PHC (2.0 mg/kg).Blood samples were collected from the femoral artery 2 h after CPB for determination of plasma diamine oxidase (DAO),D-lactate and endotoxin levels.Spleen,liver,mesenteric lymph nodes and lung were removed for biochemical analyses.Intestinal tissue ultrastructure was examined by electron microscopy.RESULTS:In the sham-operated groups,high-and low-dose-PHC had no significant impact on the levels of DAO,D-lactate and endotoxin,or the incidence of intestinal bacterial translocation (BT).Serum levels of DAO,D-lactate,endotoxin and the incidence of intestinal BT were significantly increased in the surgical groups,compared with the sham-operated groups (0.543 ± 0.061,5.697 ± 0.272,14.75 ± 2.46,and 0/40 vs 1.038 ± 0.252,9.377 ± 0.769,60.37 ± 5.63,and 30/40,respectively,all P < 0.05).PHC alleviated the biochemical and histopathological changes in a dosedependent manner.Serum levels of DAO,D-lactate,and endotoxin and the incidence of intestinal BT in the high-dose PHC group were significantly lower than in the low-dose PHC group (0.637 ± 0.064,6.972 ± 0.349,29.64 ± 5.49,and 14/40 vs 0.998 ± 0.062,7.835 ± 0.330,38.56 ± 4.28,and 6/40,respectively,all P < 0.05).CONCLUSION:PHC protects the structure and function of the intestinal mucosa from injury after CPB in rats. | Ying-Jie Sun Hui-Juan Cao Qiang Jin Yu-Gang Diao Tie-Zheng Zhang | 2011 | World Journal of Gastroenterology2011，17，16： | 35 |
| 3 | Production and genetic improvement of minor cereals in China显示文摘China is a leading country in the production of several minor cereals such as foxtail millet,Job's tears,naked oat,and naked barley.Sorghum and proso millet have also contributed greatly to Chinese agriculture.Foxtail millet,sorghum,barley,and proso millet were widely grown as major crops 60 years ago,and the reduction in their cultivation area reflects historical changes in Chinese agriculture over the past decades.Systematic germplasm collections from the 1950 s to the 1990 s gathered more than 66,690 accessions of these minor cereals,and for some of them,the Chinese germplasm collections are the largest in the world;for example,the 27,700 accessions of foxtail millet.Germplasm evaluations of each cereal species have focused mainly on drought tolerance,nutritional quality,and resistance to their main diseases.Comparisons among lines and selection of those with desirable traits were the main breeding methods for minor cereals in the 1950 s and 1960 s,but these methods were replaced by crossbreeding in the 1970 s.Newly developed cultivars have greatly changed the production situation,and many super cultivars have become milestones in crop breeding history.In this review,we describe the distribution and ecoregions,origin and domestication,and landmark varieties of several minor cereals in China.Nearly all of the minor cereals are drought-tolerant and fertilizer-efficient.The requirements for environmentally friendly crops and a more diverse food supply for humans and animals provide new opportunities to cultivate minor cereals in the drier and warmer environmental conditions that are predicted in the future. | Xianmin Diao | 2017 | The Crop Journal2017，5，2： | 38 |
| 4 | Prognostic value of systemic immune.inflammation index in patients with gastric cancer显示文摘Background:Inflammation-based indexes have been used to predict survival and recurrence in cancer patients.Systemic immune-inflammation index(Sll) was reported to be associated with prognosis in some malignant tumors.In the present study,we aimed to explore the association between Sll and the prognosis of patients with gastric cancer.Methods:We retrospectively analyzed data from 444 gastric cancer patients who underwent gastrectomy at the First Affiliated Hospital of Sun Yat-sen University between January 1994 and December 2005.Preoperative Sll was calculated.The Chi square test or Fisher's exact test was used to determine the relationship between preoperative Sll and clinicopathologic characteristics.Overall survival(OS) rates were estimated using the Kaplan-Meier method,and the effect of Sll on OS was analyzed using the Cox proportional hazards model.Receiver operating characteristic(ROC)curves were used to compare the predictive ability of Sll,NLR,and PLR.Results:Sll equal to or higher than 660 was significantly associated with old age,large tumor size,unfavorable Borrmann classification,advanced tumor invasion,lymph node metastasis,distant metastasis,advanced TNM stage,and high carcino-embryonic antigen level,high neutrophil-lymphocyte ratio,and high platelet-lymphocyte ratio(all P<0.05).High Sll was significantly associated with unfavorable prognosis(P<0.001) and Sll was an independent predictor for OS(P=0.015).Subgroups analysis further showed significant associations between high Sll and short OS in stage Ⅰ,Ⅱ,Ⅲ subgroups(all P<0.05).Sll was superior to NLR and PLR for predicting OS in patients with gastric cancer.Conclusion:Preoperative Sll level is an independent prognostic factor for OS in patients with gastric cancer. | Kang Wang Feiyu Diao Zhijun Ye Xinhua Zhang Ertao Zhai Hui Ren Tong Li Hui Wu Yulong He Shirong Cai Jianhui Chen | 2017 | Chinese Journal of Cancer2017，36，9： | 30 |
| 5 | Effects of traditional Chinese medicine on intestinal mucosal permeability in early phase of severe acute pancreatitis显示文摘背景繁体中文药广泛地被使用了因为在中国的许多年与严重尖锐胰腺炎(树液)在病人在肠的 mucosal 渗透和结果上调查了 Dachengqi 煎的效果对待尖锐 pancreatitis.We .Methods 与要求了承认到外科的特别护理单位的持续树液一起的四十个病人被注册有希望地在学习。所有在承认上随机这些病人被划分成 Dachengqi 煎组(n=20 ) 和控制组(n=20 ) 。肠的渗透被测量二个新陈代谢地惰性的标记, lactulose (L) 和甘露糖醇(M) 的吸收估计,它口头上地被管理。内毒素的浆液集中(lipopolysaccharide, LPS ) 并且到在尿(L/M ) 的甘露糖醇的 lactulose 的比率在控制组,与那些相比在所有 patients.Results 被检测尿 L/M 比率在承认(P=0.001 ) 以后在第 7 天在 Dachengqi 煎组显著地减少了。另外, LPS 的浆液集中在承认以后在第 5 和第 7 天被减少(P=0.006, P=0.008,分别地) 。多重机关机能障碍症候群( MODS )和胰腺的感染的发生与在控制组的那些相比在 Dachengqi 煎组是显著地更低的( P=0.038 , P=0.025 ,分别地) .Conclusion Dachengqi 煎可以支持肠的 mucosal 渗透和减少的恢复在与树液一起的病人的 MODS 和胰腺的感染的发生。 | CHEN Hong LI Fei JIA Jian-guo DIAO Yong-peng LI Zong-xin SUN Jia-bang | 2010 | Chinese Medical Journal2010，，12： | 25 |
| 6 | Effect of seawater on incident plane P and SV waves at ocean bottom and engineering characteristics of offshore ground motion records off the coast of southern California, USA显示文摘The effect of seawater on vertical ground motions is studied via a theoretical method and then actual offshore ground motion records are analyzed using a statistical method. A theoretical analysis of the effect of seawater on incident plane P and SV waves at ocean bottom indicate that on one hand, the affected frequency range of vertical ground motions is prominent due to P wave resonance in the water layer if the impedance ratio between the seawater and the underlying medium is large, but it is greatly suppressed if the impedance ratio is small; on the other hand, for the ocean bottom interface model selected herein, vertical ground motions consisting of mostly P waves are more easily affected by seawater than those dominated by SV waves. The statistical analysis of engineering parameters of offshore ground motion records indicate that:(1) Under the infl uence of softer surface soil at the seafl oor, both horizontal and vertical spectral accelerations of offshore motions are exaggerated at long period components, which leads to the peak spectral values moving to a longer period.(2) The spectral ratios(V/H) of offshore ground motions are much smaller than onshore ground motions near the P wave resonant frequencies in the water layer; and as the period becomes larger, the effect of seawater becomes smaller, which leads to a similar V/H at intermediate periods(near 2 s). These results are consistent with the conclusions of Boore and Smith(1999), but the V/H of offshore motion may be smaller than the onshore ground motions at longer periods(more than 5 s). | Diao Hongqi Hu Jinjun Xie Lili | 2014 | Earthquake Engineering and Engineering Vibration2014，13，2： | 24 |
| 7 | Charlson comorbidity index helps predict the risk of mortality for patients with type 2 diabetic nephropathy显示文摘研究目的:探讨Charlson合并症指数(CCI)对2型糖尿病肾病死亡率的预测作用。研究方法:建立2型糖尿病肾病研究队列,根据CCI评分将患者合并症的严重程度分为三度:轻度(CCI1–2分)、中度(CCI 3–4分)、重度(CCI≥5分)。将影响死亡率的因素及按CCI分层后的组间差异指标进行Logistic回归分析及方差分析(ANOVA)。Kaplan-Meier生存曲线分析CCI指数对生存时间及死亡率的影响。重要结论:533例2型糖尿病肾病患者纳入研究。所有患者的CCI评分均大于1,44.7%(238/533)的患者死亡。患者的死亡率随CCI评分增加而增加,CCI 1–2分患者的死亡率为21.0%(50/238),CCI 3–4分患者的死亡率为56.7%(135/238),CCI≥5分患者的死亡率为22.3%(53/238)。Logistic回归分析显示CCI评分、血红蛋白和血浆白蛋白水平是患者死亡率的预测因子(P<0.05)。方差分析结果显示,与CCI评分相对较低的患者相比,CCI评分越高的患者其血红蛋白水平较低,而血清肌酐较高,死亡率也更高。Kaplan-Meier生存曲线显示CCI评分越高的患者生存时间越短。CCI评分是一种简单易行且实用的评估疾病合并症的方法,可用于预测2型糖尿病肾病患者的死亡率。关注2型糖尿病肾病患者的合并症将有利于早期、有效治疗及改善预后。 | You-qun HUANG Rong GOU Yong-shu DIAO Qing-hua YIN Wen-xing FAN Ya-ping LIANG Yi CHEN Min WU Li ZANG Ling LI Jing ZANG Lu CHENG Ping FU Fang LIU | 2014 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2014，15，1： | 23 |
| 8 | Effects of dietary yeast β-glucan on nutrient digestibility and serum profiles in pre-ruminant Holstein calves显示文摘This study aimed to investigate the effects of dietary supplementation of yeast β-glucan on the nutrient digestibility and serum profiles in pre-ruminant Holstein calves. Forty-two neonatal Holstein calves((39.6±4.2) kg) were randomly allotted to six groups, and each was offered one of the following diets: a basal diet(control) or the basal diet supplemented with 25, 50, 75, 100 or 200 mg of yeast β-glucan kg–1 feed(dry matter basis). The basal diet consisted of a milk replacer and a starter feed. The trial lasted for 56 d. Two digestibility trials were conducted from d 14 to 20 and from d 42 to 48. Blood samples were collected on d 0, 14, 28 and 42 for serum profile analyses. On d 56, three calves from each group were slaughtered, and intestinal samples were collected to assess the villous height, crypt depth and mucosal thickness. Although feed intake was not affected by dietary treatment(P>0.05), the average daily gain(ADG) and gain-to-feed ratios were higher(P<0.05) for the calves fed 75 mg of yeast β-glucan kg–1 feed than those in the other groups. The supplementation of yeast β-glucan at 75 mg kg–1 feed increased the apparent digestibility of dry matter(DM), crude protein(CP), ether extract(EE), and phosphorus(P)(P<0.05) and the ratio of intestinal villous height to crypt depth(V/C)(P<0.05) when compared with the control group. No effects of yeast β-glucan on the serum concentrations of total protein(TP), albumin(ALB), serum urea nitrogen(SUN) and glucose(GLU) were observed(P>0.05). Compared with the control group, supplementation of yeast β-glucan decreased(P<0.05) the serum concentrations of triglycerides(TG) and total cholesterol(TC). The serum concentration of immunoglobulin G(Ig G) and immunoglobulin M(Ig M) increased quadratically(P<0.05), whereas the serum concentration of immunoglobulin A(Ig A) was unaffected by dietary treatments(P>0.05). The supplementation of yeast β-glucan stimulated the enzymatic activity of alkaline phosphatase(ALP)(P<0.05) compared with the control group. The lysozyme(LYZ) concentration increased quadratically(P<0.05) with increasing yeast β-glucan levels. The results suggested that dietary supplementation of yeast β-glucan at 75 mg kg–1 feed improved nutrient digestibility, enhanced immunity by increasing the immunoglobulin concentration and stimulating ALP, and exerted no adverse effects on metabolism in pre-ruminant calves. | MA Tao TU Yan ZHANG Nai-feng GUO Jiang-peng DENG Kai-dong ZHOU Yi YUN Qiang DIAO Qi-yu | 2015 | Journal of Integrative Agriculture2015，14，4： | 23 |
| 9 | An Insect Imaging System to Automate Rice Light-Trap Pest Identification显示文摘Identification and counting of rice light-trap pests are important to monitor rice pest population dynamics and make pest forecast. Identification and counting of rice light-trap pests manually is time-consuming, and leads to fatigue and an increase in the error rate. A rice light-trap insect imaging system is developed to automate rice pest identification. This system can capture the top and bottom images of each insect by two cameras to obtain more image features. A method is proposed for removing the background by color difference of two images with pests and non-pests. 156 features including color, shape and texture features of each pest are extracted into an support vector machine (SVM) classifier with radial basis kernel function. The seven-fold cross-validation is used to improve the accurate rate of pest identification. Four species of Lepidoptera rice pests are tested and achieved 97.5% average accurate rate. | YAO Qing LV Jun LIU Qing-jie DIAO Guang-qiang YANG Bao-jun CHEN Hong-ming TANGJian | 2012 | Journal of Integrative Agriculture2012，11，6： | 21 |
| 10 | Output feedback control of hypersonic vehicles based on neural network and high gain observer显示文摘In this paper,the output feedback control problem for a genetic hypersonic vehicle is considered under the restriction that only the vehicle's velocity and altitude are measurable. High gain observers (HGO) are utilized to provide estimation signals for unmeasurable derivatives of the vehicle's velocity and altitude. Neural network based feedforward function is designed to compensate for model uncertainties. The proposed control design require less knowledge of the hypersonic vehicle's dynamic model. A comprehensive stability analysis of the closed loop system under the output feedback control is carried to prove that the proposed control law yields semiglobal uniformly ultimately bounded tracking while keeping all the closed loop signals bounded. Numerical simulation results are presented to validate the proposed control design. | LI XinDong KIAN Bin DIAO Chen YU YanPing YANG KaiYan ZHANG Yao | 2011 | Science China(Information Sciences)2011，54，3： | 20 |
| 11 | ANALYSIS OF SEEPAGE FLOW IN A CONFINED AQUIFER WITH A STANDING COLUMN WELL显示文摘The standing column well for ground source heat pump systems is a promising technology with high efficiency and environmental benefit, where groundwater is drawn from the bottom of a well and then re-injected to its top after transferring heat with heat pumps. Heat transfer analysis of great significance and aquifer involves complex problems. Determining the groundwater seepage flow is a precondition to solve the energy equation describing the heat transfer of the system. Only when piezometric head is obtained, the seepage velocity can be determined according to Darcy’s law. In this article the groundwater seepage flow in an axial symmetrical geometry was studied under the assumption that gross groundwater flow is neglected. An analytical solution of the groundwater seepage flow for a confined aquifer was acquired by using the integral transform method, which may provide a foundation for heat transfer analysis of the standing column well system. | LI Min DIAO Nai-ren FANG Zhao-hong | 2007 | Journal of Hydrodynamics2007，19，1： | 19 |
| 12 | Automated Counting of Rice Planthoppers in Paddy Fields Based on Image Processing显示文摘A quantitative survey of rice planthoppers in paddy fields is important to assess the population density and make forecasting decisions. Manual rice planthopper survey methods in paddy fields are time-consuming, fatiguing and tedious. This paper describes a handheld device for easily capturing planthopper images on rice stems and an automatic method for counting rice planthoppers based on image processing. The handheld device consists of a digital camera with WiFi, a smartphone and an extrendable pole. The surveyor can use the smartphone to control the camera, which is fixed on the front of the pole by WiFi, and to photograph planthoppers on rice stems. For the counting of planthoppers on rice stems, we adopt three layers of detection that involve the following:(a) the first layer of detection is an AdaBoost classifier based on Haar features;(b) the second layer of detection is a support vector machine(SVM) classifier based on histogram of oriented gradient(HOG) features;(c) the third layer of detection is the threshold judgment of the three features. We use this method to detect and count whiteback planthoppers(Sogatella furcifera) on rice plant images and achieve an 85.2% detection rate and a 9.6% false detection rate. The method is easy, rapid and accurate for the assessment of the population density of rice planthoppers in paddy fields. | YAO Qing XIAN Ding-xiang LIU Qing-jie YANG Bao-jun DIAO Guang-qiang TANG Jian | 2014 | Journal of Integrative Agriculture2014，13，8： | 18 |
| 13 | 5-Hydroxymethylcytosine signatures in cell-free DNA provide information about tumor types and stages显示文摘5-Hydroxymethylcytosine (5hmC ) 是被连接了到基因规定和癌症致病的重要哺乳动物的 DNA epigenetic 修正。这里,我们用定序途径的一枝敏感化学基于标签的低输入的猎枪在传播没有房间的 DNA (cfDNA ) 探索了 5hmC 的诊断潜力。我们从七种不同癌症类型的 49 个病人定序没有房间的 5hmC 并且发现了能被用来与高精确性预言癌症类型和阶段的不同特征。明确地,我们发现肺癌症在 cfDNA 导致 5hmC 的进步全球损失,而 hepatocellular 癌和胰腺的癌症在没有房间的 hydroxymethylome 导致疾病特定的变化。我们的 proof-of-principle 结果建议没有房间的 5hmC 签名可以潜在地被使用不仅识别癌症类型而且在一些癌症追踪肿瘤舞台。 | Chun-Xiao Song Senlin Yin Li Ma Amanda Wheeler Yu Chen Yan Zhang Bin Liu Junjie Xiong Weihan Zhang Jiankun Hu Zongguang Zhou Biao Dong Zhiqi Tian Stefanie S Jeffrey Mei-Sze Chua Samuel So Weimin Li Yuquan Wei Jiajie Diao Dan Xie Stephen R Quake | 2017 | Cell Research2017，27，10： | 17 |
| 14 | Inhibition of angiogenesis and HCT-116 xenograft tumor growth in mice by kallistatin显示文摘AIM: To investigate the inhibitory effect of kallistatin (KAL) on angiogenesis and HCT-116 xenograft tumor growth. METHODS: Heterotopic tumors were induced by subcutaneous injection of 2 × 106 HCT-11 cells in mice. Seven days later, 2 × 1011 rAAV-GFP or rAAV-KAL was injected intratumorally (n = 5 for each group). The mice were sacrificed at d 28, by which time the tumors in the rAAV-GFP group had grown to beyond 5% of the total body weight. Tumor growth was measured by calipers in two dimensions. Tumor angiogenesis was determined with tumor microvessel density (MVD) by immunohistology. Tumor cell proliferation was assessed by Ki-67 staining. RESULTS: Intratumor injection of rAAV-KAL inhibited tumor growth in the treatment group by 78% (171 ± 52 mm3) at d 21 after virus infection compared to the control group (776 ± 241 mm3). Microvessel density was significantly inhibited in tumor tissues treated with rAAV-KAL. rAAV-KAL also decreased the proportion of proliferating cells (Ki-67 positive cells) in tumors compared with the control group.CONCLUSION: rAAV-mediated expression of KAL inhibits the growth of colon cancer by reducing angiogenesis and proliferation of tumor cells, and may provide a promising anti-angiogenesis-based approach to the treatment of metastatic colorectal cancer. | Yong Diao Jian Ma Wei-Dong Xiao Jia Luo Xin-Yan Li Kin-Wah Chu Peter WC Fung Nagy Habib Farzin Farzaneh Rui-An Xu | 2007 | World Journal of Gastroenterology2007，13，34： | 15 |
| 15 | A simple taurocholate-induced model of severe acute pancreatitis in rats显示文摘AIM: To investigate gut barrier damage and intestinal bacteria translocation in severe acute pancreatitis (SAP), a simple rat model of SAP was induced and studied.METHODS: Pancreatitis was induced by uniformly distributed injection of 3.8% Na taurocholate (1 mL/kg) beneath the pancreatic capsule. Rats in the control group were injected with normal saline in the identical location. RESULTS: Serum amylase, plasma endotoxin, intestinal permeability, and pancreatitis pathology scores were all markedly higher in the pancreatitis group than in the control group (P < 0.01). The bacterial infection rate was signif icantly higher in the SAP group than in the control group (P < 0.01), observed in parallel by both bacterial culture and real-time polymerase chain reaction. Acute damage of the pancreas was observed histologically in SAP rats, showing interstitial edema, leukocyte infiltration, acinar cell necrosis and hemorrhage. The microstructure of the intestinal mucosa of SAP ratsappeared to be destroyed with loose, shortened microvilli and rupture of the intercellular junction, as shown by electron microscopy. CONCLUSION: Significant gut barrier damage and intestinal bacterial translocation were def initely observed with few potential study confounders in this SAP rat model, suggesting that it may be an appropriate animal model for study of gut barrier damage and bacterial translocation in SAP. | Zhong-Hui Liu Jun-Sheng Peng Chu-Jun Li Zu-Li Yang Jun Xiang Hu Song Xiao-Bing Wu Jun-Rong Chen De-Chang Diao | 2009 | World Journal of Gastroenterology2009，15，45： | 15 |
| 16 | Nerve Growth Factor Promotes Angiogenesis and Skeletal Muscle Fiber Remodeling in a Murine Model of Hindlimb Ischemia显示文摘 | Yong-Peng Diao Feng-Kui Cui Sheng Yan Zuo-Guan Chen Li-Shan Lian Li-Long Guo Yong-Jun Li | 2016 | Chinese Medical Journal2016，，3： | 15 |
| 17 | Genetic variation of yellow pigment and its components in foxtail millet(Setaria italica(L.) P.Beauv.) from different eco-regions in China显示文摘Kernel color is an important trait for assessing the commercial and nutritional quality of foxtail millet.Yellow pigment content(YPC)and carotenoid components(lutein and zeaxanthin)of 270 foxtail millet accessions,including 50 landraces and 220improved cultivars,from four different eco-regions in China were surveyed using spectrophotometry and high performance liquid chromatography methods.Results indicated that YPC had rich variance,ranging from 1.91 to 28.54 mg kg^(–1),with an average value of 17.80 mg kg^(–1).The average YPC of improved cultivars(18.31 mg kg^(–1))was significantly higher than that of landraces(15.51 mg kg^(–1)).The YPC in cultivars from the Loess Plateau spring sowing region(LPSSR)was the highest(20.59 mg kg^(–1)),followed by the North China summer sowing region(NCSSR,18.25 mg kg^(–1)),the northeast spring sowing region(NSSR,17.25 mg kg^(–1)),and the Inner Mongolia Plateau spring sowing region(IMPSSR,13.92 mg kg^(–1)).The variation coefficients of YPC in cultivars from NSSR,LPSSR,and IMPSSR were higher than that from NCSSR.A similar carotenoid profile was also obtained for 270 foxtail millet cultivars.Lutein and zeaxanthin accounted for approximately 55–65%of YPC in accessions.The lutein content was higher than zeaxanthin content in all cultivars.The ratio of lutein to zeaxanthin ranged from 1.51 to 6.06 with an average of 3.34.YPC was positively correlated with lutein(r=0.935,P<0.01),zeaxanthin(r=0.808,P<0.01),and growth duration(r=0.488,P<0.01),whereas it was negatively correlated with grain protein(r=-0.332,P<0.01)and 1 000-kernel weight(r=-0.153,P<0.05).Our study is useful for screening and selecting cultivars with high levels of yellow pigment and for enhancing phytochemical concentrations in breeding programs. | YANG Yan-bing JIA Guan-qing DENG Li-gang QIN Ling CHEN Er-ying CONG Xin-jun ZOU Ren-feng WANG Hai-lian ZHANG Hua-wen LIU Bin GUAN Yan-an DIAO Xian-min YIN Yan-ping | 2017 | Journal of Integrative Agriculture2017，16，11： | 15 |
| 18 | Melittin induces human gastric cancer cell apoptosis via activation of mitochondrial pathway显示文摘AIM: To investigate the apoptotic effects of melittin on SGC-7901 cells via activation of the mitochondrial signaling pathway in vitro.METHODS: SGC-7901 cells were stimulated by melittin, and its effect on proliferation and apoptosis of was investigated by methyl thiazolyl tetrazolium assay, morphologic structure with transmission electron microscopy, annexin-V/propidium iodide double-staining assay, measuring mitochondrial membrane potential(MMP) levels, and analyzing reactive oxygen species(ROS) concentrations were analyzed by flow cytometry. Cytochrome C(Cyt C), apoptosis-inducing factor(AIF), endonuclease G(Endo G), second mitochondria-derived activator of caspases(Smac)/direct IAP binding protein with low isoelectric point(Diablo), and FAS were analyzed by western blot. The expression of caspase-3 and caspase-8 was measured using activity assay kits.RESULTS: Melittin was incubated at 1.0, 2.0, 4.0, or 6.0 μg/m L for 1, 2, 4, 6, or 8 h and showed a timeand concentration-dependent inhibition of SGC-7901 cell growth. Melittin induced SGC-7901 cell apoptosis, which was confirmed by typical morphological changes. Treatment with 4 μg/m L melittin induced early apoptosis of SGC-7901 cells, and the early apoptosis rates were 39.97% ± 3.19%, 59.27% ± 3.94%, and 71.50% ± 2.87% vs 32.63% ± 2.75% for 1, 2, and 4 h vs 0 h(n = 3, P < 0.05); the ROS levels were 616.53% ± 79.78%, 974.81% ± 102.40%, and 1330.94% ± 93.09% vs 603.74% ± 71.99%(n = 3, P < 0.05); the MMP values were 2.07 ± 0.05, 1.78 ± 0.29, and 1.16 ± 0.25 vs 2.55 ± 0.42(n = 3, P < 0.05); caspase-3 activity was significantly higher compared to the control(5492.3 ± 321.1, 6562.0 ± 381.3, and 8695.7 ± 449.1 vs 2330.0 ± 121.9), but the caspase activity of the non-tumor cell line L-O2 was not different from that of the control. With the addition of the caspase-3 inhibitor(Ac-DEVD-CHO), caspase-3 activity was significantly decreased compared to the control group(1067.0 ± 132.5 U/g vs 8695.7 ± 449.1 U/g). The expression of the Cyt C, Endo G, and AIF proteins in SGC-7901 cells was significantly higher than those in the control(P < 0.05), while the expression of the Smac/Diablo protein was significantly lower than the control group after melittin exposure(P < 0.01). Ac-DEVD-CHO did not, however, have any effect on the expression of caspase-8 and FAS in the SGC-7901 cells.CONCLUSION: Melittin can induce apoptosis of human gastric cancer(GC) cells through the mitochondria pathways, and it may be a potent agent in the treatment of human GC. | Gui-Mei Kong Wen-Hua Tao Ya-Li Diao Peng-Hua Fang Ji-Jun Wang Ping Bo Feng Qian | 2016 | World Journal of Gastroenterology2016，22，11： | 15 |
| 19 | Influence of Ren and Du meridian electro-acupuncture on neural stem cell proliferation and extracellular signal-regulated kinase pathway in a rat model of focal cerebral ischemia injury显示文摘BACKGROUND:Studies have shown that electro-acupuncture at the Ren meridian could improve proliferation of subventricular zone neural stem cells in cerebral-ischemic rats. However,there are few reports on the influence of electro-acupuncture at the Du meridian on neural stem cell proliferation. OBJECTIVE:To observe the influence of electro-acupuncture at Ren and Du meridians on neural stem cell proliferation in the subventricular zone and altered signal transduction in cerebral ischemia rats. DESIGN,TIME AND SETTING:A randomized,controlled,animal experiment was performed at the Laboratory of Human Anatomy,Medical College of Sun Yat-sen University from May 2006 to February 2008. MATERIALS:Mouse anti-rat bromodeoxyuridine (BrdU) monoclonal antibody was provided by Sigma,USA; mouse anti-rat nestin monoclonal antibody and extracellular signal-regulated protein kinase (ERK) specific inhibitor PD98059 were provided by Calbiochem,Germany; acupuncture needle was provided by Suzhou Acupuncture Supplies,China. METHODS:A total of 126 rats were randomly assigned to four groups:model (n = 36),Du meridian (n = 36),Ren/Du meridian (n = 36),and Ren/Du meridian + PD98059 (n = 18). Rats in the Ren /Du meridian + PD98059 group were observed on days 7 (n = 6) and 14 (n = 12) after cerebral ischemia injury. Rats in the model,Du meridian,and Ren/Du meridian groups were observed on days 7,14,and 28 after cerebral ischemia injury,with 12 rats per group at each time point. Thread occlusion was used to establish middle cerebral artery occlusion models. Electro-acupuncture was performed at Renzhong (DU 26) and Baihui (DU 20) acupoints in the Du meridian group,as well as Chengjiang (RN 24),Guanyuan (RN 4),Renzhong,and Baihui acupoints in the Ren/Du meridian and Ren/Du meridian + PD98059 groups 2 days after model establishment. In addition,electro-acupuncture stimulation with disperse-dense waves was performed,with 30 Hz disperse wave,100 Hz dense wave,and 5 V intensity for 20 minutes. Rats in the Ren/Du meridian + PD98059 group were treated with 0.2 μg PD98059 injection into the subventricular zone,2 μL per rat. Rats in the model group were not treated with electro-acupuncture. MAIN OUTCOME MEASURES:BrdU/nestin immunofluorescent staining was used to detect proliferating neural stem cells in the subventricular zone of cerebral ischemia rats; Western blot was used to determine phosphorylated ERK1 and 2 (pERK1/2) expression in the subventricular zone. RESULTS:On days 14 and 28 after cerebral ischemia,there were significantly more BrdU-positive and BrdU/nestin-positive cells in the Ren /Du meridian group compared with the Du meridian group (P < 0.05). PD98059 decreased the number of BrdU-positive and BrdU/nestin-positive cells induced by electro-acupuncture at the Ren and Du meridians (P < 0.05). On days 7,14,and 28 after treatment,pERK1/2 expression was significantly greater in the Du meridian and Ren/Du meridian groups compared with the model group (P < 0.05). The promoting effect of electro-acupuncture at Ren and Du meridians on ERK1/2 phosphorylation was superior to electro-acupuncture at the Du meridian alone on day 14 after model induction (P < 0.05). However,PD98059 completely abolished the promoting effect of electro-acupuncture at Ren/Du meridians on pERK1/2 expression (P < 0.05). CONCLUSION:Electro-acupuncture at Ren and Du meridians increased proliferation of subventricular zone neural stem cells,which was related to activation of the ERK pathway in a rat model of cerebral ischemia injury. | Wenshu Luo Haibo Yu Zhuoxin Yang Min Pi Lihong Diao Xiaodan Rao | 2010 | Neural Regeneration Research2010，5，6： | 14 |
| 20 | Protection of the liver against CCl_4-induced injury by intramuscular electrotransfer of a kallistatin-encoding plasmid显示文摘AIM:To investigate the effect of transgenic expression of kallistatin(Kal) on carbon tetrachloride(CCl 4)induced liver injury by intramuscular(im) electrotransfer of a Kal-encoding plasmid formulated with poly-Lglutamate(PLG).METHODS:The pKal plasmid encoding Kal gene was formulated with PLG and electrotransferred into mice skeletal muscle before the administration of CCl 4.The expression level of Kal was measured.The serum biomarker levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),malonyldialdehyde(MDA),and tumor necrosis factor(TNF)-α were monitored.The extent of CCl 4-induced liver injury was analyzed histopathologically.RESULTS:The transgene of Kal was sufficiently expressed after an im injection of plasmid formulated with PLG followed by electroporation.In the Kal gene-transferred mice,protection against CCl 4-induced liver injury was reflected by significantly decreased serum ALT,AST,MDA and TNF-α levels compared to those in control mice(P < 0.01 to 0.05 in a dose-dependent manner).Histological observations also revealed that hepatocyte necrosis,hemorrhage,vacuolar change and hydropic degeneration were apparent in mice after CCl 4 administration.In contrast,the damage was markedly attenuated in the Kal gene-transferred mice.The expression of hepatic fibrogenesis marker transforming growth factor-β1 was also reduced in the pKal transferred mice.CONCLUSION:Intramuscular electrotransfer of plasmid pKal which was formulated with PLG significantly alleviated the CCl 4-induced oxidative stress and inflammatory response,and reduced the liver damage in a mouse model. | Yong Diao Xiao-Feng Zhao Jun-Sheng Lin Qi-Zhao Wang Rui-An Xu | 2011 | World Journal of Gastroenterology2011，17，1： | 14 |
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