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| 1 | Effect of artificially accelerated aging on the vigor of Metasequoia glyptostroboides seeds显示文摘Metasequoia glyptostroboides is an endangered relict plant native to China that has been widely introduced into many countries and areas around the world. However,its seeds germinate at a very low percentage. Consequently,population regeneration by seed is low under natural conditions, which probably contributes to the endangered status of this plant species. The present study aimed to describe the aging mechanism of M. glyptostroboides seeds. Our objective was to elucidate causes of the low germination rate in an effort to enhance potential for conservation of the species. We used germination tests,relative electrical conductivity and malondialdehyde content determination, ultrastructural observation of embryo cells, and analysis of superoxide dismutase, catalase,ascorbate peroxidase, and dehydroascorbate reductase activities during accelerated aging treatment. We found that M. glyptostroboides seeds have a low level of vigorand poor ability to maintain vigor, which is probably associated with the inefficiency of its enzymatic antioxidative system. | Huan Liu Yanfang Zhu Xia Liu Yu Jiang Shiming Deng Xunru Ai Zhijun Deng | 2020 | Journal of Forestry Research2020，31，3： | 5 |
| 2 | De novo assembly of a Tibetan genome and identification of novel structural variants associated with high-altitude adaptation显示文摘Structural variants(SVs) may play important roles in human adaptation to extreme environments such as high altitude but have been under-investigated. Here, combining long-read sequencing with multiple scaffolding techniques, we assembled a high-quality Tibetan genome(ZF1), with a contig N50 length of 24.57 mega-base pairs(Mb) and a scaffold N50 length of 58.80 Mb. The ZF1 assembly filled 80 remaining N-gaps(0.25 Mb in total length) in the reference human genome(GRCh38). Markedly, we detected17 900 SVs, among which the ZF1-specific SVs are enriched in GTPase activity that is required for activation of the hypoxic pathway. Further population analysis uncovered a 163-bp intronic deletion in the MKL1 gene showing large divergence between highland Tibetans and lowland Han Chinese. This deletion is significantly associated with lower systolic pulmonary arterial pressure, one of the key adaptive physiological traits in Tibetans. Moreover, with the use of the high-quality de novo assembly, we observed a much higher rate of genome-wide archaic hominid(Altai Neanderthal and Denisovan) shared non-reference sequences in ZF1(1.32%–1.53%) compared to other East Asian genomes(0.70%–0.98%),reflecting a unique genomic composition of Tibetans. One such archaic hominid shared sequence-a662-bp intronic insertion in the SCUBE2 gene-is enriched and associated with better lung function(the FEV1/FVC ratio) in Tibetans. Collectively, we generated the first high-resolution Tibetan reference genome, and the identified SVs may serve as valuable resources for future evolutionary and medical studies. | Ouzhuluobu Yaoxi He Haiyi Lou Chaoying Cui Lian Deng Yang Gao Wangshan Zheng Yongbo Guo Xiaoji Wang Zhilin Ning Jun Li Bin Li Caijuan Bai Baimakangzhuo Gonggalanzi Dejiquzong Bianba Duojizhuoma Shiming Liu Tianyi Wu Shuhua Xu Xuebin Qi Bing Su | 2020 | National Science Review2020，7，2： | 4 |
| 3 | Molecular cloning and characterization of antimicrobial peptides from skin of Hylarana guentheri显示文摘把抗菌剂肽(安培) 编码为 Hylarana guentheri 的皮肤的 cDNAs 被识别,也就是 temporin (五肽,称为的 temporin-GHa-GHd 和 temporin-GUa ) , brevinin-1 (一肽, brevinin-1GUb ) ,并且 brevinin-2 (八肽, brevinin-2GHd-2GHj,和 brevinin-2GHb ) 。14 肽中的十一个有新奇主要结构。综合 temporin GHa-GHd 有用途广泛的抗菌剂对克积极的细菌(葡萄球菌 aureus 和杆菌 subtilis ) 的活动,克否定的细菌(Escherichia coli, Vibrio alginolyticus,和 Pseudomonas aeruginosa ) ,以及真菌(Candida albicans ) 。在这些测试紧张之中, S。aureus 对有禁止的集中(MIC ) 在 6.8 和 12.9 M 之间珍视的最小的 temporin-GHa-GHd 最敏感。他们也对 Methicillin 抵抗的 S 展出了抗菌剂活动。有从 12.7 ~ 51.7 M 的 MIC 的 aureus。有趣地,第二等的结构预言证明在 temporin-GHb 没有螺旋,它说明那个螺旋没为 temporin-GHb 的抗菌剂活动被要求。NaCl (在 0.15-2 M 的最后的集中) 减少 temporin-GHa-GHd 的抗菌剂活动稍微,当时人的浆液和 S。aureus V8 朊酶没在抗菌剂活动有效果。扫描 E 的电子显微镜学图象。coli 和 S。aureus 证明微生物引起的房间的表面在有在 37 畲獧的 temporin-GHa-GHd 的处理的 1 h 以后更加不平、忏悔吗? | Zhu Dong Wenjie Luo Hengren Zhong Manchuriga Wang Yanting Song Shiming Deng Yingxia Zhang | 2017 | Acta Biochimica et Biophysica Sinica2017，49，5： | 3 |
| 4 | A simula- tion study on the operating performance of a solar-air source heat pump water heater显示文摘 | Xu Guoying Zhang Xiaosong Deng Shiming | 2006 | Applied Thermal En- gineering2006，26，： | 1 |
| 5 | A study on the performance of a geothermal heat exchanger under coupled heat conduction and groundwater advection显示文摘 | Rui Fan Yiqiang Jiang Yang Yao Deng Shiming Zuiliang Ma | 2007 | Energy2007，，11： | 1 |
| 6 | A Study on the Performance of the Airside Heat Exchanger Under Frosting in an Air Soure Heat Pump Water/Chiller Unit 显示文摘 | Yang Yao Yiqiang Jiang Shiming Deng | 2004 | International Journal of Heat and Mass Transfer2004，47，1718： | 1 |
| 7 | A study of the re- verse cycle defrosting performance on a multi-circuit out- door coil unit in an air source heat pump - Part I:Ex- periments 显示文摘 | Qu Minglu Xia Liang Deng Shiming | 2012 | Applied Energy2012，91，1： | 1 |
| 8 | A study on the operational stability of a refrigeration system having a variable speed compressor 显示文摘 | Yiming Chen Shiming Deng Xiangguo Xu | 2008 | International Journal of Refrigeration2008，，31： | 1 |
| 9 | Theoretical analysis of low- temperature hot souree driven two-stage LiBr/H20 absorption refrigeration system 显示文摘 | Ma Wenbo Deng Shiming | 1996 | International Journal of Refrigeration1996，19，2： | 1 |
| 10 | A Simulation Study on A Solar Heat Pump Heating System with Seasonal Latent Heat Storage 显示文摘 | Qi Qi Shiming Deng Yiqiang Jiang | 2008 | Solar Energy2008，8，： | 1 |
| 11 | A study on the performance of the airside heat exchanger under frosting in an air source heat pump water heater/chiller unit显示文摘 | Yang Yao Yiqiang Jiang Shiming Deng | 2004 | International Journal of Heat and Mass Transfer2004，47，： | 1 |
| 12 | A simulation study on the operating performance of a solar-air source heat pump water heater显示文摘 | Xu Guoying Zhang Xiaosong Deng Shiming | 2006 | Applied Thermal Engineering2006，26，： | 1 |
| 13 | The outdoor air ventilation rate in high-rise residences employing room air conditioners显示文摘 | Zhongping Lin Shiming Deng | 2003 | Building and Environment2003，38，： | 1 |
| 14 | A simulation study on the operating performance of a solar-alr source heat pump water heater显示文摘 | Xu Guoying Zhang Xiaosong Deng Shiming | 2006 | Applied Thermal Engineering2006，21，2： | 1 |
| 15 | Comparative Nutritional Analysis on Fish Meal and Meat and Bone Meal of Harmless Treatment of Dead Pig Carcass显示文摘In order to explore the resource utilization of the harmless treatment product(pork meat and bone meal,abbreviated PM)of pigs died of non-communicable diseases,the general nutritional components and amino acid composition of PM and fish meal were determined and compared.The results showed that the contents of moisture,crude protein,crude fat and ash in PM and fish meal were 3.25%and 8.92%,66.65%and 66.67%,13.52%and 8.23%,18.25%and 21.50%,respectively.The contents of essential amino acids(EAA)in PM and fish meal were 19.94%and 22.35%,respectively.For PM and fish meal,the first limiting amino acid was Met(methionine)+Cys(cysteine),and the second limiting amino acid was Lys(lysine);their essential amino acid indexes(EAAI)were 66.60 and 77.04,respectively;and their delicious amino acid(DAA)contents were 26.89%and 23.15%,respectively.In summary,the meat and bone meal of pigs died of non-communicable diseases has the characteristics of high protein and low ash contents,and has certain development and utilization potential as a recycled waste resource,especially in aquatic feed to replace fish meal. | Xiaofei CHENG Jin XIANG Shiming DENG Xing TIAN Xiping YUAN Shaoming LI Rui SONG Li LIU | 2020 | Agricultural Biotechnology2020，9，6： | 1 |
| 16 | A study on the operational stability of a refrigeration system having a variable speed compressor 显示文摘 | Chen Yiming Deng Shiming Xu Xiangguo | 2008 | International Journal of Refrigeration2008，31，： | 1 |
| 17 | Energy use and management in hotels in Hong Kong显示文摘 | Deng Shiming John Burnett | 2002 | International Journal of Hospitality Management2002，，4： | 1 |
| 18 | Expression of EphrinB2 and EphB4 in glioma tissues correlated to the progression of glioma and the prognosis of glioblastoma patients显示文摘 | Yanyang Tu Shiming He Jianfang Fu Gang Li Ruxiang Xu Hongliu Lu Jianping Deng | 2012 | Clinical and Translational Oncology2012，，3： | 1 |
| 19 | A study on the performance on the airside heat exchanger under frosting in air-source heat pump water heater/chiller unit 显示文摘 | Yao Yang Jiang Yiqiang Deng Shiming | 2004 | International Journal of Heat and Mass Transfer2004，47，1718： | 1 |
| 20 | A simulation study on a solar heat pump heating system with sea- sonal latent heat storage 显示文摘 | Qi Qi Deng Shiming Jiang Yiqiang | 2008 | Solar Energy2008，82，： | 1 |
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