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| 1 | Molecular mechanisms of liver ischemia reperfusion injury:Insights from transgenic knockout models显示文摘Ischemia reperfusion injury is a major obstacle in liver resection and liver transplantation surgery.Understanding the mechanisms of liver ischemia reperfusion injury(IRI) and developing strategies to counteract this injury will therefore reduce acute complications in hepatic resection and transplantation,as well as expanding the potential pool of usable donor grafts.The initial liver injury is initiated by reactive oxygen species which cause direct cellular injury and also activate a cascade of molecular mediators leading to microvascular changes,increased apoptosis and acute inflammatory changes with increased hepatocyte necrosis.Some adaptive pathways are activated during reperfusion that reduce the reperfusion injury.IRI involves a complex interplay between neutrophils,natural killer T-cells cells,CD4+ T cell subtypes,cytokines,nitric oxide synthases,haem oxygenase-1,survival kinases such as the signal transducer and activator of transcription,Phosphatidylinositol 3-kinases/Akt and nuclear factor κβ pathways.Transgenic animals,particularly genetic knockout models,have become a powerful tool at elucidating mechanisms of liver ischaemia reperfusion injury and are complementary to pharmacological studies.Targeted disruption of the protein at the genetic level is more specific and maintained than pharmacological inhibitors or stimulants of the same protein.This article reviews the evidence from knockout models of liver IRI about the cellular and molecular mechanisms underlying liver IRI. | Gourab Datta Barry J Fuller Brian R Davidson | 2013 | World Journal of Gastroenterology2013，19，11： | 50 |
| 2 | Bt水稻对主要非靶标害虫和蜘蛛优势种田间种群动态的影响显示文摘研究了2个转cry1Ab+cry1Ac杂合基因籼稻品系TT9-3和T19-4对主要非靶标害虫飞虱、叶蝉以及捕食性蜘蛛优势种田间种群动态的影响。结果表明,Bt稻与亲本对照(IR72)田间白背飞虱和黑尾叶蝉成虫的虫口密度在整个水稻生育期间无显著差异,而若虫密度有一定的不同。TT9-3稻田中白背飞虱和黑尾叶蝉若虫密度分别于移栽后早中期和中后期显著高于对照;TT9-4与对照间无显著差异。Bt水稻与亲本对照间锥腹肖蛸、四斑锯螯蛛、食虫沟瘤蛛、拟环纹豹蛛及拟水狼蛛种群数量动态趋势相似,数量上大多无显著差异。综合分析认为,Bt水稻对稻田优势蜘蛛基本上无明显的负影响。 | 刘志诚 叶恭银 胡萃 Swapan Kumar Datta | 2002 | 植物保护学报2002，29，2： | 46 |
| 3 | Bt水稻对飞虱和叶蝉及其卵寄生蜂扩散规律的影响显示文摘研究了Bt水稻两个纯合品系TT9-3和TT9-4在田间对飞虱、叶蝉及其卵寄生蜂扩散规律的影响。结果表明:在Bt稻区与对照(IR72)区间相互扩散的飞虱有白背飞虱(Sogatellafurcifea)、褐飞虱(Nilaparvatalugens);叶蝉有黑尾叶蝉(Nephotetixcincticeps)、白翅叶蝉(Erythroneurasubrufa)和电光叶蝉(Deltpcephalusdorsalis);其中以白背飞虱和黑尾叶蝉为主。扩散数量叶蝉多于飞虱;扩散方向以Bt稻区向对照区扩散为主,其中TT9-3与IR72、TT9-4与IR72间白背飞虱与黑尾叶蝉的扩散量差异均达显著水平(P<0.05)。在Bt稻区与对照区间相互扩散的飞虱和叶蝉的卵寄生蜂有稻虱缨小蜂(Anagrusspp.)和叶蝉柄翅小蜂(Lynaenonlongicrus),数量上后者多于前者,扩散方向也以Bt稻区向对照区扩散为主。 | 陈茂 叶恭银 胡萃 Tu J Datta S K | 2003 | 浙江大学学报(农业与生命科学版)2003，29，1： | 30 |
| 4 | 以基因枪介导获转ps1-barnase基因的工程雄性不育水稻植株显示文摘以ps1-barnase(brn)为目的基因,pHcintG(PG)为选择/标记基因进行共转化,以PDS-1000-氦气基因枪介导,将brn及PG基因转化到水稻台北309及秋光的核DNA中,得到了转ps1-barnase基因的工程雄性不育植株。以悬浮细胞作为基因枪轰击的靶材料,转化植株再生频率较初级愈伤组织的为高。转brn基因植株的其他主要性状与供体亲本无显著差异,但却表现不育。其不育的程度在不同的植株之间表现不同。在转brn基因植株中观察到全不育(占全部brn阳性植株的40.6%)、高不育(占15.6%)及半不育的个体(占43.7%)。全不育的转基因植株自交完全不能结实(结实率为零),除个别植株外,花粉完全不被I-KI染色;而人工授以正常的花粉则可以获得杂交种子。而brn基因的阴性植株及未进行转化的对照植株则完全可育,表明转基因植株之雄性不育乃brn基因所致。结果表明,brn基因在水稻中是完全可以正常表达的,其表达的时期推测在花粉母细胞减数分裂前至花粉形成之间的整个时期。 | 凌定厚 陶利珍 马镇荣 S P Zhang S K Datta | 1998 | Acta Genetica Sinica1998，25，5： | 23 |
| 5 | Effects of Silicon-Based Fertilizer on Growth, Yield and Nutrient Uptake of Rice in Tropical Zone of Vietnam显示文摘Application of silicon(Si) could greatly boost rice yield and mitigate abiotic stress,especially drought.A field experiment was conducted during 2015 at the research farm of Hong Duc University,Thanh Hoa City,Vietnam,to evaluate the effects of five different combined doses of standard fertilizer practice and Si fertilizer on growth,yield and yield components,as well as nutrient uptake of rice.The treatments consisted of the recommended dose of fertilizer(RDF,110 kg/hm^2 N + 90 kg/hm^2 P_2O_5 + 80 kg/hm^2 K_2O) as the control,RDF + 100 kg/hm^2 SiO_2,RDF + 200 kg/hm^2 SiO_2,RDF + 300 kg/hm^2 SiO_2 and RDF + 400 kg/hm^2 SiO_2.The results showed that the growth,grain and straw yields as well as yield components(number of grains per panicle,seed-setting rate and 1000-grain weight) were significantly affected by Si application.The highest grain yield of 3 705 kg/hm^2 was obtained with the highest level of Si fertilizer in combination with RDF(RDF + 400 kg/hm^2 SiO_2),however,it was statistically at par with the yields obtained with RDF + 300 kg/hm^2 SiO_2(3 664 kg/hm^2) and RDF + 200 kg/hm^2 SiO_2(3 621 kg/hm^2).The optimum dose of Si fertilizer with maximized grain yield(3 716 kg/hm^2) was 329 kg/hm^2 SiO_2.The nutrient(Si,N,P and K) uptakes of rice were also significantly enhanced by Si application.Si application at the level of 329 kg/hm^2 along with RDF would help in the sustainable production of rice in the tropical zone of Vietnam. | Tran Xuan CUONG Hayat ULLAH Avishek DATTA Tran Cong HANH | 2017 | Rice science2017，24，5： | 16 |
| 6 | 战略决策过程:批判性回顾与未来研究展望显示文摘本文在对以往文献进行回顾的基础上,构建了一个战略决策过程的整合框架。该框架将决策过程特征的前置因素(环境因素、组织因素和决策专有因素)及其过程结果和经济结果整合在一起。依据该框架,本文对重要的实证研究文献进行了综述,指出了主要的研究模式以及研究成果中的矛盾之处。在综述的基础上,文章还探讨了本研究对于理论构建、研究方法和管理实践的启示,并指出了未来研究的一些方向。 | Nandini Rajagopalan Abdul M. A. Rasheed Deepak K. Datta 苗莉(译) | 2012 | 管理世界2012，28，1： | 15 |
| 7 | Osteoporotic fracture and parathyroid hormone显示文摘Osteoporosis and age-related bone loss is associated with changes in bone remodeling characterized by decreased bone formation relative to bone resorption,resulting in bone fragility and increased risk of fractures.Stimulating the function of bone-forming osteoblasts,is the preferred pharmacological intervention for osteoporosis.Recombinant parathyroid hormone(PTH),PTH(1-34),is an anabolic agent with proven benefits to bone strength and has been characterized as a potential therapy for skeletal repair.In spite of PTH’s clinical use,safety is a major consideration for long-term treatment.Studies have demonstrated that intermittent PTH treatment enhances and accelerates the skeletal repair process via a number of mechanisms.Recent research into the molecular mechanism of PTH action on bone tissue has led to the development of PTH analogs to control osteoporotic fractures.This review summarizes a number of advances made in the field of PTH and bone fracture to combat these injuries in humans and in animal models.The ultimate goal of providing an alternative to PTH,currently the sole anabolic therapy in clinical use,to promote bone formation and improve bone strength in the aging population is yet to be achieved. | Nabanita S Datta | 2011 | World Journal of Orthopedics2011，2，8： | 14 |
| 8 | Salt Stress in Arabidopsis: Lipid Transfer Protein AZI1 and Its Control by Mitogen-Activated Protein Kinase MPK3显示文摘应付环境挑战的一个植物能力大部分依靠通过 mitogen-activatedprotein kinase (MAPK ) 的传播串联的信号。在 Arabidopsis thaliana, MPK3 强烈特别地与众多的不能生活、关於生命的压力回答被联系。MPK3 substratesis 的鉴定向在植物改进压力抵抗的一个里程碑。这里,我们描绘 AZI1,类脂化合物转移蛋白质(LTP )-relatedhybrid 充满脯氨酸的蛋白质(HyPRP ) , MPK3 的一个新奇目标。AZI1 是由在 vitro 的 MPK3 的 phosphorylated。作为记录 byco-immunoprecipitation 和双分子的荧光互补实验, AZI1 与 MPK3 交往在 planta 形成 proteincomplexes。而且, azi1 的空异种是过分敏感的腌应力,当 AZI1-overexpressing 线是时显著地更容忍。在基因背景部分减轻的 mpk3 的 AZI1 overexpression 这变异,却功能的 MPK3 的盐过分敏感的显型出现为授与 AZI1 的坚韧性的最大程度要求的 tobe。尤其是,这坚韧性不在损坏 normaldevelopment 的情况下来。Immunoblot 和 RTPCR 数据指向 AZI1 abundance.Key 词的 MPK3 同样积极的管理者的一个角色: | Andrea Pitzschke Sneha Datta Helene Persak | 2014 | Molecular Plant2014，7，4： | 13 |
| 9 | Hepatitis viruses and non-Hodgkin's lymphoma: A review显示文摘Non-Hodgkin's lymphoma(NHL) is among the haematological malignancies with high prevalence worldwide, causing estimated 355 900 new cases and 191 400 deaths in 2008. High prevalence of NHL is documented in economically more developed areas while low prevalence is observed in less developed areas of the globe. A wide array of environmental factors have been reported to be either directly involved or in modifying the risk of NHL development. In addition to these factors, a number of infectious agents, chiefly viruses have also been implicated in the development of NHL. This article reviews the available literature to discuss the role of hepatitis viruses in NHL development, possible mechanisms of lymphomagenesis and also identify the areas in which further research is required to better understand this disease. A brief discussion on the clinical aspects such as classification, staging, treatment approaches have also been included in this article. | Sibnarayan Datta Soumya Chatterjee Rudragoud S Policegoudra Hemant K Gogoi Lokendra Singh | 2012 | World Journal of Virology2012，1，6： | 11 |
| 10 | Loss of disabled-2 expression is an early event in esophageal squamous tumorigenesis显示文摘AIM: Disabled-2 (DAB2) is a candidate tumor-suppressor gene identified in ovarian cancer that negatively influences mitogenic signal transduction of growth factors and blocks ras activity. In a recent study, we observed down-regulation of DAB2 transcripts in ESCCs using cDNA microarrays. In the present study, we aimed to determine the clinical significance of loss of DAB2 protein in esophageal tumorigenesis, hypothesizing that DAB2 promoter hypermethylation-mediated gene silencing may account for loss of the protein. METHODS: DAB2 expression was analyzed by immunohistochemistry in 50 primary esophageal squamous cell carcinomas (ESCCs), 30 distinct hyperplasia, 15 dysplasia and 10 non-malignant esophageal tissues. To determine whether promoter hypermethylation contributes to loss of DAB2 expression in ESCCs, methylation status of DAB2 promoter was analyzed in DAB2 immuno-negative tumors using methylation-specifi c PCR. RESULTS: Loss of DAB2 protein was observed in 5/30 (17%) hyperplasia, 10/15 (67%) dysplasia and 34/50 (68%) ESCCs. Significant loss of DAB2 protein was observed from esophageal normal mucosa to hyperplasia, dysplasia and invasive cancer (Ptrend < 0.001). Promoter hypermethylation of DAB2 was observed in 2 of 10 (20%) DAB2 immuno-negative ESCCs. CONCLUSION: Loss of DAB2 protein expression occurs in early pre-neoplastic stages of development of esophageal cancer and is sustained down the tumorigenic pathway. Infrequent DAB2 promoter methylation in ESCCs suggests that epigenetic genesilencing is only one of the mechanisms causing loss of DAB2 expression in ESCCs. | Kumar Anupam Chatopadhyay Tusharkant Siddhartha Datta Gupta Ralhan Ranju | 2006 | World Journal of Gastroenterology2006，12，37： | 11 |
| 11 | Bt籼稻对褐飞虱取食、产卵行为的影响显示文摘比较了褐飞虱在2个Bt籼稻纯合品系,即TT9-3、TT9-4,及亲本对照IR72上的取食和产卵行为。结果表明,在选择性条件下,褐飞虱于稻株上的着虫比率在Bt籼稻与对照间无显著差异,而稻株中部和整个叶鞘的取食刺探痕数、产卵选择比率和产卵量则均以Bt籼稻显著为低。在非选择性条件下,稻株叶鞘上的取食刺探痕数在Bt籼稻与对照间无显著差异,而排泄的蜜露量则多以Bt籼稻尤其是TT9-4显著为少。可见,供试的2个Bt籼稻品系不利于褐飞虱的取食或产卵,表现有抗褐飞虱的能力。 | 陈茂 叶恭银 胡萃 Datta S.K | 2003 | 植物保护学报2003，30，4： | 9 |
| 12 | Significant increase in HBV, HCV, HIV and syphilis infections among blood donors in West Bengal, Eastern India 2004-2005: Exploratory screening reveals high frequency of occult HBV infection显示文摘AIM: To evaluate the prevalence of markers of hepatitis B virus (HBV) and hepatitis C virus (HCV) and human immunodeficiency virus (HIV) among blood donors in Kolkata, Eastern India for two consecutive years and to conduct a pilot study to explore the presence of HBV DNA among hepatitis B surface antigen (HBsAg) negative but anti-HBc positive blood donors. METHODS: Seroprevalence of HBsAg, anti-HCV and anti-HIV was studied among 113 051 and 106 695 voluntary blood donors screened in 2004 and 2005, respectively. Moreover, a pilot study on 1027 HBsAg negative donors was carried out for evaluating the presence of HBV DNA by PCR on HBsAg negative/anti- HBc positive donors. RESULTS: A statistically significant increase in the prevalence of HBV (1448 vs 1768, P < 0.001), HIV (262 vs 374, P < 0.001), HCV (314 vs 372, P = 0.003) and syphilis (772 vs 853, P = 0.001) infections was noted among blood donors of Kolkata West Bengal in 2005 as compared to 2004. Moreover, the exploratory study on 1027 HBsAg negative donors revealed that 188 (18.3%)of them were anti-HBc positive out of which 21% were positive for HBV DNA. CONCLUSION: The findings of this study underscore the significantly increasing endemicity of hepatitis viruses, syphilis and HIV among the voluntary blood donors of our community. The pilot study indicates a high rate of prevalence of HBV DNA among HBsAg negative/anti-HBc positive donors and thus emphasizes the need for a more sensitive and stringent screening algorithm for blood donations. | Prasun Bhattacharya Partha Kumar Chandra Sibnarayan Datta Arup Banerjee Subhashish Chakraborty Krishnan Rajendran Subir Kumar Basu Sujit Kumar Bhattacharya Runu Chakravarty | 2007 | World Journal of Gastroenterology2007，13，27： | 9 |
| 13 | Extraordinary evanescent field confinement waveguide sensor for mid-infrared trace gas spectroscopy显示文摘Nanophotonic waveguides are at the core of a great variety of optical sensors.These structures confine light along defined paths on photonic chips and provide light-matter interaction via an evanescent field.However,waveguides still lag behind free-space optics for sensitivity-critical applications such as trace gas detection.Short optical pathlengths,low interaction strengths,and spurious etalon fringes in spectral transmission are among the main reasons why on-chip gas sensing is still in its infancy.In this work,we report on a mid-infrared integrated waveguide sensor that successfully addresses these drawbacks.This sensor operates with a 107%evanescent field confinement factor in air,which not only matches but also outperforms free-space beams in terms of the per-length optical interaction.Furthermore,negligible facet reflections result in a flat spectral background and record-low absorbance noise that can finally compete with free-space spectroscopy.The sensor performance was validated at 2.566μm,which showed a 7 ppm detection limit for acetylene with only a 2 cm long waveguide. | Marek Vlk Anurup Datta Sebastián Alberti Henock Demessie Yallew Vinita Mittal Ganapathy Senthil Murugan Jána Jagerská | 2021 | Light(Science & Applications)2021，10，2： | 9 |
| 14 | To be or not to be:The host genetic factor and beyond in Helicobacter pylori mediated gastro-duodenal diseases显示文摘Helicobacter pylori(H.pylori)have long been associated with a spectrum of disease outcomes in the gastroduodenal system.Heterogeneity in bacterial virulence factors or strains is not enough to explain the divergent disease phenotypes manifested by the infection.This review focuses on host genetic factors that are involved during infection and eventually are thought to influence the disease phenotype.We have summarizedthe different host genes that have been investigated for association studies in H.pylori mediated duodenal ulcer or gastric cancer.We discuss that as the bacteria co-evolved with the host;these host gene also show much variation across different ethnic population.We illustrate the allelic distribution of interleukin-1B,across different population which is one of the most popular candidate gene studied with respect to H.pylori infections.Further,we highlight that several polymorphisms in the pathway gene can by itself or collectively affect the acid secretion pathway axis(gastrin:somatostatin)thereby resulting in a spectrum of disease | Dipanjana Datta De Susanta Roychoudhury | 2015 | World Journal of Gastroenterology2015，21，10： | 8 |
| 15 | RNAi-Mediated Silencing of ITPK Gene Reduces Phytic Acid Content,Alters Transcripts of Phytic Acid Biosynthetic Genes,and Modulates Mineral Distribution in Rice Seeds显示文摘Phytic acid is the principal storage form of phosphorus in plant seeds and an essential signalling molecule in several regulatory processes of plant development.However,it is known as an anti-nutrient compound owing to its potent chelating property.Thus,reducing the phytic acid content in crops is desirable.Studies involving regulation of MIPS and IPK1 genes to generate low phytate rice have been reported earlier.However,the functional significance of OsITPK and the effect of its down-regulation on phytic acid content and the associated pleiotropic effects on rice have not yet been investigated.In this study,tissue specific RNA interference(RNAi)-mediated down-regulation of a major ITPK homolog(OsITP5/6K-1)resulted in 46.2%decrease in phytic acid content of T2 transgenic seeds with a subsequent 3-fold enhancement in the inorganic phosphorus content.Silencing of OsITP5/6K-1 altered the transcript levels of essential phytic acid pathway genes,without significantly affecting the transcript levels of other OsITPK homologs.Furthermore,the mapping of elements through X-ray microfluorescence analysis revealed significant changes in the spatial distribution pattern and translocation of elements in low phytate seeds.Additionally,low phytate polished seeds exhibited 1.3-fold and 1.6-fold enhancement in iron and zinc content in the grain endosperm,respectively.Silencing of OsITP5/6K-1 also altered the amino acid and myo-inositol content of the transgenic seeds.Our results successfully established that RNAi-mediated silencing of OsITP5/6K-1 gene significantly reduced the phytate levels in seeds without hampering the germination potential of seeds and plant growth.The present study provided an insight into the mechanism of phytic acid biosynthesis pathway. | Aritra KARMAKAR Sananda BHATTACHARYA Shinjini SENGUPTA Nusrat ALI Sailendra Nath SARKAR Karabi DATTA Swapan K.DATTA | 2020 | Rice science2020，27，4： | 7 |
| 16 | Non-alcoholic fatty liver disease may not be a severe disease at presentation among Asian Indians显示文摘瞄准:与非含酒精的脂肝疾病(NAFLD ) 评估病人的临床、生物化学的侧面并且在表示估计他们的组织学的严厉。方法:介绍给所有印度的肝诊所的连续病人与提起的 transaminases 医学科学(AIIMS ) 开创到乘正常的上面的限制的至少 1.5,并且组织学地证实的非酒精的脂肝疾病被包括。或谁有重要白酒吸入或另外的肝疾病的积极标记的病人正在服知道生产脂肝的药被排除。这个组的临床、生物化学、组织学的侧面被学习。结果:有 NAFLD 的 51 个病人形成了学习人口。他们的中部的年龄和 BMI 是 34 (17-58 ) 年并且 26.7 (21.3-32.5 ) kg/m (2 ) 分别地并且(90.1%) 46 是男性。病人的多数有温和发炎,也分级 1 [32 (63%)] 或等级 2 [16 (31%)] 并且仅仅 3 (6%) 病人们有严重(等级 3 ) 发炎。23 (45%) , 19 (37%) , 8 (16%) 并且 1 (2%) 病人有 0, 1, 2 和 3 纤维变性分别地在索引活体检视和没有上有的舞台肝硬化。在 univariate 分析上,甘油三酸酯铺平超过 150 mg %( 或 = 7.1;95% CI:1.6-31.5, P = 0.002 ) 并且著名计算机生产厂商 / 中高音 ratio>1 (或 = 14.3;95% CI:1.4-678.5, P = 0.008 ) 与发炎的高等级被联系,任何一个都没与先进纤维变性被联系。在上多变量逻辑回归分析, hypertriglyceridemia >150 mg% 是独立地与发炎的高等级的存在联系的唯一的因素(或 = 1.6;95% CI:1.3-22.7, P = 0.02 ) ,当任何一个都没与先进纤维变性被联系时。甘油三酸酯层次与煽动性的等级断然相关(r = 0.412;P = 0.003 ) 。结论:在诺思印第安人病人的 NAFLD 是年轻超重男性,其中大多数是抵抗的胰岛素的疾病,他们趋于在表示有温和组织学的疾病。 | Kaushal Madan Yogesh Batra S Datta Gupta Bal Chander K D Anand Rajan M S Tewatia S K Panda S K Acharya | 2006 | World Journal of Gastroenterology2006，12，21： | 7 |
| 17 | Light signaling and UV-B-mediated plant growth regulation^FA显示文摘Light plays an important role in plants' growth and development throughout their life cycle. Plants alter their morphological features in response to light cues of varying intensity and quality. Dedicated photoreceptors help plants to perceive light signals of different wave-lengths. Activated photoreceptors stimulate the down-stream signaling cascades that lead to extensive gene expression changes responsible for physiological and developmental responses. Proteins such as ELONGATED HYPOCOTYL5 (HY5) and CONSTITUTIVELY PHOTO-MORPHOGENIC 1 (COP1) act as important factors which modulate light-regulated gene expression, especially during seedling development. These factors function as central regulatory intermediates not only in red, far-red, and blue light pathways but also in the UV-B signaling pathway. UV-B radiation makes up only a minor fraction of sunlight, yet it imparts many positive and negative effects on plant growth. Studies on UV-B perception, signaling, and response in plants has considerably surged in recent times. Plants have developed different strat-egies to use UV-B as a developmental cue as well as to withstand high doses of UV-B radiation. Plants' re-sponses to UV-B are an integration of its cross-talks with both environmental factors and phytohormones. This review outlines the current developments in light sig-naling with a major focus on UV-B-mediated plant growth regulation. | Arpita Yadav Deeksha Singh Maneesh Lingwan Premachandran Yadukrishnan Shyam Kumar Masakapalli Sourav Datta | 2020 | Journal of Integrative Plant Biology2020，62，9： | 6 |
| 18 | Recent advances in molecular diagnostics of hepatitis Bvirus显示文摘Hepatitis B virus(HBV)is one of the important global health problems today.Infection with HBV can lead to a variety of clinical manifestations including severe hepatic complications like liver cirrhosis and hepatocellular carcinoma.Presently,routine HBV screening and diagnosis is primarily based on the immuno-detection of HBV surface antigen(HBsAg).However,identification of HBV DNA positive cases,who do not have detectable HBsAg has greatly encouraged the use of nucleic acid amplification based assays,that are highly sensitive,specific and are to some extent tolerant to sequence variation.In the last few years,the field of HBV molecular diagnostics has evolved rapidly with advancements in the molecular biology tools,such as polymerase chain reaction(PCR)and real-time PCR.Recently,apart of PCR based amplification methods,a number of isothermal amplification assays,such as loop mediated isothermal amplification,transcription mediated amplification,ligase chain reaction,and rolling circle amplification have been utilized for HBV diag-nosis.These assays also offer options for real time detection and integration into biosensing devices.In this manuscript,we review the molecular technologies that are presently available for HBV diagnostics,with special emphasis on isothermal amplification based technologies.We have also included the recent trends in the development of biosensors and use of next generation sequencing technologies for HBV. | Sibnarayan Datta Soumya Chatterjee Vijay Veer Molecular Virology Laboratory | 2014 | World Journal of Gastroenterology2014，20，40： | 6 |
| 19 | Molecular Biology of the Hepatitis B Virus for Clinicians显示文摘 | Sibnarayan Datta Soumya Chatterjee Vijay Veer Runu Chakravarty | 2012 | Journal of Clinical and Experimental Hepatology2012，，4： | 5 |
| 20 | Next-generation sequencing in clinical virology: Discovery of new viruses显示文摘Viruses are a cause of significant health problem world-wide, especially in the developing nations. Due to different anthropological activities, human populations are exposed to different viral pathogens, many of which emerge as outbreaks. In such situations, discovery of novel viruses is utmost important for deciding prevention and treatment strategies. Since last century, a number of different virus discovery methods, based on cell culture inoculation, sequence-independent PCR have been used for identification of a variety of viruses. However, the recent emergence and commercial availability of nextgeneration sequencers(NGS) has entirely changed the field of virus discovery. These massively parallel sequencing platforms can sequence a mixture of genetic materials from a very heterogeneous mix, with high sensitivity. Moreover, these platforms work in a sequenceindependent manner, making them ideal tools for virus discovery. However, for their application in clinics, sample preparation or enrichment is necessary to detect low abundance virus populations. A number of techniques have also been developed for enrichment or viral nucleic acids. In this manuscript, we review the evolution of sequencing; NGS technologies available today as well as widely used virus enrichment technologies. We also discuss the challenges associated with their applications in the clinical virus discovery. | Sibnarayan Datta Raghvendra Budhauliya Bidisha Das Soumya Chatterjee Vanlalhmuaka Vijay Veer | 2015 | World Journal of Virology2015，4，3： | 5 |
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