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| 1 | Current perspectives on in vitro maturation and its effects on oocyte genetic and epigenetic profiles显示文摘In vitro maturation(IVM), the maturation in culture of immature oocytes, has been used in clinic for more than 20 years.Although IVM has the specific advantages of low cost and minor side effects over controlled ovarian stimulation, the prevalence of IVM is less than 1% of routine in vitro fertilization and embryo transfer techniques in many reproductive centers. In this review, we searched the MEDLINE database for all full texts and/or abstract articles published in English with content related to oocyte IVM mainly between 2000 and 2016. Many different aspects of the IVM method may influence oocyte potential,including priming, gonadotrophin, growth factors, and culture times. The culture conditions of IVM result in alterations in the oocyte or cumulus cell transcriptome that are not observed under in vivo culture conditions. Additionally, epigenetic modifications, such as DNA methylation or acetylation, are also different between in vitro and in vivo cultured oocytes. In sum,current IVM technique is still not popular and requires more systematic and intensive research to improve its effects and applications. This review will help point our problems, supply evidence or clues for future improving IVM technique, thus assist patients for fertility treatment or preservation as an additional option. | Cuiling Lu Yaoyao Zhang Xiaoying Zheng Xueling Song Rui Yang Jie Yan Huailiang Feng Jie Qiao | 2018 | Science China(Life Sciences)2018，61，6： | 10 |
| 2 | Structural basis for the neutralization of hepatitis E virus by a cross-genotype antibody显示文摘肝炎 E 病毒(HEV ) ,一个非包的、积极感觉的、搁浅单人赛的 RNA 病毒,是伤寒肝炎的一个主要原因。分类进家庭 Hepeviridae, HEV 包括四遗传型(遗传型 1-4 ) ,它属于单个 serotype。我们描述 monoclonal 抗体(mAb ) ,有 ~ 2.53-3.45 nM 的 8G12,同等地认出 HEV 的所有四遗传型,有约束力的亲密关系。mAb 8G12 有一保护,抵销能力,它能显著地在主人房间堵住病毒感染。动物与 1, 3 和 4 证实了跨遗传型的抵销的遗传型学习 8G12 和它肝炎 E 疾病的有效预防的能力。有丰富的遗传型的 HEV E2s 领域(病毒衣壳的最耸出的区域) 的 8G12 的复杂水晶结构 1 和 4 在 4.0 和 2.3 Å 被决定;分辨率分别地。这些结构表明 8G12 在 E2s dimerization 区域通过 epitopes 认出两遗传型。有像病毒的粒子的基于结构的 mutagenesis 和房间模型试金识别了为 8G12 中立化是必要的几保存残余(Glu549, Lys554 和 Gly591 ) 。而且, 8G12 的 epitope 作为涉及病毒主人相互作用的关键 epitope 被识别。这些调查结果将帮助为 HEV 感染的最丰富的形式的预防开发普通策略。 | Ying Gu Xuhua Tang Xiao Zhang Cuiling Song Minghua Zheng Kaihang Wang Jun Zhang Mun-Hon Ng Choy-Leong Hew Shaowei Li Ningshao Xia J. Sivaraman | 2015 | Cell Research2015，25，5： | 3 |
| 3 | Screening of abiotic stress-responsive cotton genes using a cotton full-length cDNA overexpressing Arabidopsis library显示文摘Cotton(Gossypium hirsutum L.)is a major crop and the main source of natural fiber worldwide.Because various abiotic and biotic stresses strongly influence cotton fiber yield and quality,improved stress resistance of this crop plant is urgently needed.In this study,we used Gateway technology to construct a normalized full-length cDNA overexpressing(FOX)library from upland cotton cultivar ZM12 under various stress conditions.The library was transformed into Arabidopsis to produce a cotton-FOX-Arabidopsis library.Screening of this library yielded 6,830 transgenic Arabidopsis lines,of which 757 were selected for sequencing to ultimately obtain 659 cotton ESTs.GO and KEGG analyses mapped most of the cotton ESTs to plant biological process,cellular compo-nent,and molecular function categories.Next,156potential stress-responsive cotton genes were identified from the cotton-FOX-Arabidopsis library under drought,salt,ABA,and other stress conditions.Four stress-related genes identified from the library,designated as GhCAS,GhAPX,GhSDH,and GhPOD,were cloned from cotton complementary DNA,and their expression patterns under stress were analyzed.Phenotypic experiments in-dicated that overexpression of these cotton genes in Arabidopsis affected the response to abiotic stress.The method developed in this study lays a foundation for high-throughput cloning and rapid identification of cotton functional genes. | Shengting Li Hao Chen Zhi Hou Yu Li Cuiling Yang Daojie Wang Chun-Peng Song | 2020 | Journal of Integrative Plant Biology2020，62，7： | 0 |
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