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| 1 | Platelet aggregation but not activation and degranulation during the acute post-ischemic reperfusion phase in livers with no underlying disease显示文摘Background:Platelets and P-selectin(CD62P)play an unequivocal role in the pathology of hepatic ischemia/reperfusion(I/R)injury.Inhibition or knock-out of P-selectin or immunodepletion of platelets results in amelioration of post-ischemic inflammation,reduced hepatocellular damage,and improved survival.However,P-selectin expression on platelets and endothelial cells,which concurs with platelet activation,has never been clearly demonstrated in I/R-subjected livers.Aims:To determine whether platelets become activated and degranulate in the acute phase of liver I/R and whether the platelets interact with neutrophils.Methods:Hepatic I/R was induced in male C57BL/6J mice(N=12)using 37.5-min ischemia time.Platelets,endothelial cells,and neutrophils were fluorescently labeled by systemic administration of non-blocking antibodies.Cell kinetics were monitored by intravital spinning disk confocal microscopy during 90 min of reperfusion.Image analysis and quantification was performed with dedicated software.Results:Platelets adhered to sinusoids more extensively in post-ischemic livers compared to livers not subjected to I/R and formed aggregates,which occurred directly after ischemia.Platelets and endothelial cells did not express P-selectin in post-ischemic livers.There was no interaction between platelets and neutrophils.Conclusions:Platelets aggregate but do not become activated and do not degranulate in post-ischemic livers.There is no platelet-neutrophil interplay during the early reperfusion phase in a moderate model of hepatic I/R injury.The mechanisms underlying the biological effects of platelets and P-selectin in this setting warrant further investigation.Relevance for patients:I/R in surgical liver patients may compromise outcome due to post-ischemic oxidative stress and sterile inflammation.Both processes are mediated in part by platelets.Understanding platelet function during I/R is key to developing effective interventions for I/R injury and improving clinical outcomes. | Rowan F.van Golen Katarzyna M.Stevens Pina Colarusso Hartmut Jaeschke Michal Heger | 2015 | Journal of Clinical & Translational Research2015，1，2： | 2 |
| 2 | Rapid detection of influenza A and B viruses in clinical specimens by LightCycler real-time RT-PCR显示文摘 | Smith A B Mock V Melear R Colarusso P Willis D E | 2003 | J Clin Virol2003，28，： | 1 |
| 3 | Molecular and isotopic records of C4 grassland expansion in the Late Miocene显示文摘 | Freeman K H Colarusso L A | | 0，，09： | 1 |
| 4 | Altered -tubulin isotype expression in paclitaxel resistant human prostate carcinoma cells显示文摘 | Ranganathan S Benetatos C A Colarusso P J | 1998 | Br J Cancer1998，77，4： | 1 |
| 5 | Raytheon delivers first track illuminator laser for airborne laser显示文摘 | Colarusso L M | 2002 | Inside Missile Defense2002，8，24： | 1 |
| 6 | Altered-tubulin isotype expression in paclitaxel re- sistant human prostate carcinoma cells显示文摘 | Ranganathan S Benetatos CA Colarusso PJ | 2008 | Br J Cancer2008，77，4： | 1 |
| 7 | Intraluminal crawling of neutrophils to emigration sites: a molecularly distinct process from adhesion in the recruitment cascade 显示文摘 | Phillipson M Heit B Colarusso P | 2006 | J Exp Med2006，203，12： | 1 |
| 8 | PI3K accelerates, but is not required for, nentrophil chemotaxis to fMLP 显示文摘 | Heir B Liu L Colarusso P | 2008 | J Cell Sci2008，121，： | 1 |
| 9 | Alpha-ketoacids are potent slow binding inhibitors of the hepatitis C virus NS3 protease显示文摘 | Narjes F Brunetti M Colarusso S | 2000 | Biochemistry2000，39，7： | 1 |
| 10 | Intraluminal crawling of neutrophils to emigration sites: a molecularly distinct process from adhesion in the recruitment cascade显示文摘 | Phillipson M Heit B Colarusso P | 2006 | Journal of Experimental Medicine2006，203，12： | 1 |
| 11 | Middle to Late Paleozoic Atmospheric CO2 Levels from Soil Carbonate and Organic Matter显示文摘 | Mora C I Driese S G Colarusso L | 1996 | Science1996，271，： | 1 |
| 12 | Current management of differentiated thyroid carcinoma显示文摘 | Lupoli G A Fonderico F Colarusso S | 2005 | Med Sei Monit2005，11，12： | 1 |
| 13 | Molecular and isotopic records of C4 grassland expansion in the late Miocene 显示文摘 | Freeman K H Colarusso L A | 2001 | Geochimica et Cosmochimica Acta2001，65，： | 1 |
| 14 | The role of capsid- endothelial interactions in the innate immune response to adcnovirus vectors 显示文摘 | Iju Q Zaiss AK Colarusso P | 2003 | Hum Gene Ther2003，14，7： | 1 |
| 15 | Evolu- tion, synthesis and sar of tripeptide α-ketoacid inhibitors of the hepatitis C virus NS3/NS4 a serine protease显示文摘 | COLARUSSO S GERLACH B KOCH U | 2002 | Bioor- ganie and medicinal chemistry letters2002，12，： | 1 |
| 16 | Observed prevalence of congenital heart defects from a surveillance study in China 显示文摘 | Zhang Y Riehle - Colarusso T Correa A | 2011 | J Ul- trasound Med2011，30，7： | 1 |
| 17 | SAR and pharmacokinetic studies on phenethylamide inhibitors of the hepatitis C virus NS3/4A serine protease 显示文摘 | Malancona S Colarusso S Ontoria JM | 2004 | Bioorg Med Chem Lett2004，14，17： | 1 |
| 18 | Sonoehemical oxidation phenol and three of its intermediate products in aqueous media: Catechol, hydroquinone and benzo- quinone, kinetic and mechanistic aspects显示文摘 | Serpone N R Terzan P Colarusso O Minerco C Pelizzeti E | 1992 | Res Chem Interme- diates1992，18，23： | 1 |
| 19 | Molecular and isotopic records of C4 grassland expansion in the late Miocene显示文摘 | Freeman K H Colarusso L A | 2001 | Geochimica et Cosmochimica Acta2001，65，9： | 1 |
| 20 | Effect of Weight Loss by Gastric Bypass Surgery Versus Hypocaloric Diet on Glucose and Incretin Levels in Patients with Type 2 Diabetes显示文摘 | Blandine Laferrère Julio Teixeira James McGinty Hao Tran Joseph R. Egger Antonia Colarusso Betty Kovack Baani Bawa Ninan Koshy Hongchan Lee Kimberly Yapp Blanca Olivan | 2008 | The Journal of Clinical Endocrinology & Metabolism2008，，7： | 1 |
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