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| 1 | Protective action of glutamine in rats with severe acute liver failure显示文摘BACKGROUND Severe acute liver failure(SALF) is a rare, but high-mortality, rapidly evolving syndrome that leads to hepatocyte degeneration with impaired liver function.Thioacetamide(TAA) is a known xenobiotic, which promotes the increase of the formation of reactive oxygen species. Erythroid 2-related factor 2(Nrf2) activates the antioxidant protection of cells. Studies have evidenced the involvement of inflammatory mediators in conditions of oxidative stress.AIM To evaluate the antioxidant effects of glutamine on Nrf2 activation and NFκBmediated inflammation in rats with TAA-induced IHAG.METHODS Male Wistar rats(n = 28) were divided into four groups: control,control+glutamine, TAA, and TAA + glutamine. Two TAA doses(400 mg/kg)were administered intraperitoneally, 8 h apart. Glutamine(25 mg/kg) was administered at 30 min, 24 h, and 36 h. At 48 h, blood was collected for liver integrity analysis [aspartate aminotransferase(AST), alanine aminotransferase(ALT), and alkaline phosphatase(ALP)]. The liver was harvested for histology and assessment of oxidative stress [thiobarbituric acid-reactive substances(TBARS), catalase(CAT), glutathione peroxidase(GPx), glutathione S-transferase(GST), glutathione(GSH), Nrf2, Kelch-like ECH-associated protein 1(Keap1),NADPH quinone oxidoreductase1(NQO1), superoxide dismutase(SOD)] and inflammatory process.RESULTS TAA caused disruption of the hepatic parenchyma, with inflammatoryinfiltration, massive necrosis, and ballooning degeneration. Glutamine mitigated this tissue damage, with visible regeneration of hepatic parenchyma; decreased TBARS(P < 0.001), GSH(P < 0.01), IL-1β, IL6, and TNFα levels(P <0.01) in hepatic tissue; and decreased blood levels of AST, ALT, and ALP(P <0.05). In addition, CAT, GPx, and GST activities were restored in the glutamine group(P<0.01, P <0.01, and P <0.001, respectively vs TAA alone). Glutamine increased expression of Nrf2(P < 0.05), NQO1, and SOD(P < 0.01), as well as levels of IL-10(P <0.001), while decreasing expression of Keap1, TLR4, NFκB(P < 0.001), COX-2 and iNOS,(P < 0.01), and reducing NO_2 and NO_3 levels(P < 0.05).CONCLUSION In the TAA experimental model of IHAG, glutamine activated the Nrf2 pathway,thus promoting antioxidant protection, and blunted the NFκB-mediated pathway, reducing inflammation. | Elizangela G Schemitt Renata M Hartmann Josieli R Colares Francielli Licks Jéferson O Salvi Cláudio A Marroni Norma P Marroni | 2019 | World Journal of Hepatology2019，11，3： | 5 |
| 2 | Content levels of various fruit metabolites in the 'Conference' pear response to branch ben- ding显示文摘 | COLARIC M STAMPAR F HUDINA M | 2007 | Scientia Horticulturae2007，113，3： | 1 |
| 3 | Behavior of natural fiber in stone matrix asphalt mixtures using two design methods显示文摘 | VALE D COLARES A CASAGRANDE M et a| | 2014 | Journal of Materials in Civil Engineering2014，26，3： | 1 |
| 4 | Traditional walnut liqueur – cocktail of phenolics 显示文摘 | Stampar F Solar A Hudina M Veberic R Colaric M | 2006 | Food Chem2006，95，4： | 1 |
| 5 | Stability of hypersurfaces with constant r-mean curvature显示文摘 | Barbosa J L M Colares A G | 1997 | Annals of Global Analysis and Geometry1997，15，： | 1 |
| 6 | Pleiotropic effects of statins on the treatment of chronic periodontitis – a systematic review显示文摘 | Ilanna Mara Gomes Estanislau Icrólio Ribeiro Colares Terceiro Mario Roberto Pontes Lisboa Patrícia de Barros Teles Rosimary de Sousa Carvalho Ricardo Souza Martins Maria M?nica Studart Mendes Moreira | 2015 | Br J Clin Pharmacol2015，，： | 1 |
| 7 | Bending affects pheno-lic content of William pear leaves显示文摘 | COLARIC M STAMPAR F HUDINA M | 2007 | Plant Soil Science2007，57，2： | 1 |
| 8 | Seasonal variations of se- lected flavonoids, phenolic acids and quinones in annual shoots of com- mon walnut (Juglans regia L)显示文摘 | SOLAR A COLARIC M USENIK V | 2006 | Plant Sci2006，170，3： | 1 |
| 9 | Leaf epidermal characteristics of Lotus subgenus Acmlspon (Fabaceae: Loteae) and a numerical taxonomic evaluation 显示文摘 | Stenglein S A Arambarrl A M Colares M N | 2003 | Canadian Journal of Botany2003，81，： | 1 |
| 10 | Rigidity Results for Submanifolds with Parallel Mean Curvature Vector in the de Sitter Space显示文摘 | Brasil A Chaves R M B Colares A G | 2006 | Glasgow Mathematical Journal2006，48，1： | 1 |
| 11 | Evaluation of peach and nectarine fruit quality and correlations between sensory and chemical attributes显示文摘 | Colaric M Veberic R Stampar F | 2005 | J Sci Food Agric2005，85，15： | 1 |
| 12 | Behavior of natural fiber in stone matrix asphalt mixtures using two design methods 显示文摘 | Vale D Colares A Casagrande M | 2014 | Journal of Materials in Civil Engi- neering2014，26，3： | 1 |
| 13 | Melatonin prevents oxidative stress,inflammatory activity,and DNA damage in cirrhotic rats显示文摘BACKGROUND Cirrhosis is an important health problem characterized by a significant change in liver parenchyma.In animals,this can be reproduced by an experimental model of bile duct ligation(BDL).Melatonin(MLT)is a physiological hormone synthesized from serotonin that has been studied for its beneficial properties,including its antioxidant potential.AIM To evaluate MLT’s effects on oxidative stress,the inflammatory process,and DNA damage in an experimental model of secondary biliary cirrhosis.METHODS Male Wistar rats were divided into 4 groups:Control(CO),CO+MLT,BDL,and BDL+MLT.MLT was administered(20 mg/kg)daily beginning on day 15 after biliary obstruction.On day 29 the animals were killed.Blood samples,liver tissue,and bone marrow were collected for further analysis.RESULTS BDL caused changes in biochemical and histological parameters and markers of inflammatory process.Thiobarbituric acid(0.46±0.01)reactive substance levels,superoxide dismutase activity(2.30±0.07)and nitric oxide levels(2.48±0.36)were significantly lower(P<0.001)n the groups that received MLT.DNA damage was also lower(P<0.001)in MLT-treated groups(171.6±32.9)than the BDL-only group(295.5±34.8).Tissue damage and the expression of nuclear factor kappa B,interleukin-1β,Nrf2,NQO1 and Hsp70 were significantly lower in animals treated with MLT(P<0.001).CONCLUSION When administered to rats with BDL-induced secondary biliary cirrhosis,MLT effectively restored the evaluated parameters. | Josieli R Colares Renata M Hartmann Elizângela G Schemitt Sandielly R B Fonseca Marilda S Brasil Jaqueline N Picada Alexandre S Dias Aline F Bueno Cláudio A Marroni Norma P Marroni | 2022 | World Journal of Gastroenterology2022，28，3： | 1 |
| 14 | Evaluation of peach and nectarine fruit quality and correlations between sensory and chemical attributes 显示文摘 | COLARIC M VEBERIC R STAMPAR F | 2005 | Journal of the Science of Food and Agriculture2005，85，： | 1 |
| 15 | Traditional walnut liqueur-cocktail of phenolics显示文摘 | STAMPAR F SOLAR A HUDINA M VEBERIC R COLARIC M | 2006 | Food Chemistry2006，95，： | 1 |
| 16 | Phenolic acids, syringaldehyde and juglone in fruits of different cultivars of juglans regia L显示文摘 | Colaric M Veberic R | 2005 | Agric Food Chem2005，53，： | 1 |
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